scholarly journals Introductory studies of bacterial effects on the course of mitosis in adventitious roots of Allium cepa L.

2015 ◽  
Vol 41 (2) ◽  
pp. 205-216
Author(s):  
J. Cebrat ◽  
K. Czerwińska ◽  
K. Bohdanowicz-Murek
Keyword(s):  
Allium Cepa ◽  
Chromosome Aberrations ◽  
Adventitious Roots ◽  
Water Culture ◽  
Meristematic Cells ◽  
Allium Cepa L ◽  
Spontaneous Chromosome

The level of spontaneous chromosome aberrations and other phenomena concomitant with mitoses in the meristematic cells of <i>Allium cepa</i> L. adventitious roots grown in water, depends to a large extent on the intensity of bacteria multiplication. From the water culture two strains of bacteria, which were most numerous, were isolated - <i>Agrobacterium</i> and <i>Flavobacterium</i>. A supernatant from bacteria grown on Davis medium induced chromosome sticking together, c-mitoses and the formation of polyploid nuclei in the roots of onion.

scholarly journals On the effects of secretions of saprophytic bacteria on the course of mitosis in adventitious roots of Allium cepa L.

2015 ◽  
Vol 42 (1) ◽  
pp. 171-179
Author(s):  
Z. Kobierzyńska
Keyword(s):  
Allium Cepa ◽  
Chromosome Aberrations ◽  
Adventitious Roots ◽  
Bacterial Strains ◽  
Liquid Cultures ◽  
Allium Cepa L

The influence of 21 strains of saprophytic bacteria isolated from onion cultures on the course of mitosis and on the level of chromosome aberrations in adventitious roots of <i>Allium cepa</i> L. was studied. Liquid cultures of all bacterial strains caused no changes in divisions of the cells. However, ten of the strains were responsible for disturbances in this process. The nature of these disturbances depended to a large extent on the kind of medium in which the bacteria were grown.

scholarly journals Antiproliferative effect of the hydroalcoholic extract of Hymenaea stigonocarpa Mart. ex Hayne (Fabaceae, Caesalpinioideae) on the meristematic cells of Allium cepa L. roots

Biotemas ◽  
2014 ◽  
Vol 28 (1) ◽  
pp. 45 ◽  
Author(s):  
Layana Menezes da Silva ◽  
Ana Paula Peron ◽  
Francisco Ronielson da Silva Carvalho ◽  
Livia Martins ◽  
Iana Felício Bantim Calou ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Antiproliferative Effect ◽  
Hydroalcoholic Extract ◽  
Meristematic Cells ◽  
Allium Cepa L

scholarly journals Influence of 1-p-D-arabinofuranosylcytosine on mitotic activity of apical meristem of onion (Allium cepa L.) roots

2015 ◽  
Vol 47 (1–2) ◽  
pp. 173-187 ◽  
Author(s):  
M. Kuraś ◽  
A. Malinowska
Keyword(s):  
Mitotic Activity ◽  
Chromosome Aberration ◽  
Allium Cepa ◽  
Cytosine Arabinoside ◽  
Adventitious Roots ◽  
Apical Meristem ◽  
Allium Cepa L ◽  
Cells With Micronuclei

The influence of increasing cytosine arabinoside (ara-C) concentration (50, 100, 300 and 500 μg/ml) on the mitotic activity of the apical meristem of onion adventitious roots was investigated during 24-h incubation in ara-C and postincubation in water. Incubation in ara-C inhibits reversibly mitosis, the degree of inhibition being dependent on the concentration used. 50 μg/ml ara-C causes only a slight and transitional mitotic depression, while 100—500 μg/ml reduces the per cent of mitoses in various degrees after 12-h incubation whereas after 24 h all concentrations (100—500) reduce mitosis to the same level of about 2 per cent (80% inhibition). During postincubation in water there occurs after treatment with 100—500 μg/ml ara-C, a wave of highly synchronized mitoses with a peak after 12 h. The highest frequency of synchronized mitoses (60°/o) appeared in roots treated with 300 μg/ml ara-C. The main wave is preceded by a small one with a peak after 2-4 h of postincubation. This seems to be the consequence of phases S and G<sub>2</sub> inhibition by ara-C. It was found that under the influence of ara-C the per cent of mitoses with chromosome aberration and of cells with micronuclei increased only slightly.

scholarly journals Influence of extract from shoots of Taxus baccata var. elegantissima on ultrastructure and tubulin cytoskeleton of meristematic cells of Allium cepa L. roots

2014 ◽  
Vol 71 (3) ◽  
pp. 211-221 ◽  
Author(s):  
Agnieszka Majewska ◽  
Mirosława Furmanowa ◽  
Kazimierz Głowniak ◽  
Joanna Guzewska ◽  
Alicja Zobel ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Chromatin Condensation ◽  
Preprophase Band ◽  
Taxus Baccata ◽  
Cell Nuclei ◽  
Root Tips ◽  
Meristematic Cells ◽  
Allium Cepa L ◽  
Interphase Cells ◽  
Average Size

We investigated the influence of extract from <em>Taxus baccata</em> var. Elegantissima (TbE) shoots in 1:8 dilution, containing paclitaxel in concentration of 81,6 µg/g fresh mass on ultrastructure and tubulin cytoskeleton of meristematic cells of <em>Allium cepa</em> L. root tips. Incubation time 3, 6, 12 and 24 h was followed with postincubation in water for 12 and 24 h. During shorter incubation (till 12 h) the surface of the cell nuclei decreased and chromatin became condensed (in comparison to control) but after 24 h the average surface increased and chromatin condensation decreased. In the course of incubation the average size of plastids and vacuoles increased. Moreover, after treatment mitochondria and plastids showed degradation of ultrastructure, which was reversed after 12 h of postincubation. Immunocytochemical assays demonstrated that in the course of incubation in the ThE extract, the tubulin cytoskeleton became partially disorganised. In most interphase cells, cortical microtubules (MTs) lost their oval transverse orientation. The preprophase band (PPB) position in the cell was often asymmetrical. The MTs array of the karyokinetic spindle and phragmoplast was also disturbed. These alterations were completely reversed during postincubation.

Comparison of the Effects of He:Neon and Nd:YAG Laser Radiation on Mitotic Activity of Meristematic Cells of Allium cepa L. Bulbs

1990 ◽  
Vol 8 (6) ◽  
pp. 35-38 ◽  
Author(s):  
M. Fiol de Cuneo ◽  
S. Rubiales de Barioglio ◽  
G. Stutz ◽  
H. Juri ◽  
J. L. Lacuara
Keyword(s):  
Laser Radiation ◽  
Mitotic Activity ◽  
Allium Cepa ◽  
Nd:Yag Laser ◽  
Meristematic Cells ◽  
Allium Cepa L

Effects of anthocyanin-rich extract from red cabbage leaves on meristematic cells of Allium cepa L. roots treated with heavy metals

2007 ◽  
Vol 68 (3) ◽  
pp. 343-350 ◽  
Author(s):  
Sława Glińska ◽  
Magdalena Bartczak ◽  
Sylwia Oleksiak ◽  
Aneta Wolska ◽  
Barbara Gabara ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Allium Cepa ◽  
Red Cabbage ◽  
Meristematic Cells ◽  
Allium Cepa L

scholarly journals ON THE NATURE OF SISTER-CHROMATID EXCHANGES IN 5-BROMODEOXYURIDINE-SUBSTITUTED CHROMOSOMES

Genetics ◽  
1979 ◽  
Vol 92 (4) ◽  
pp. 1251-1264
Author(s):  
J B Schvartzman ◽  
F Cortés ◽  
A González-Fernández ◽  
C Gutiérrez ◽  
J F LÓpez-Sáez
Keyword(s):  
Allium Cepa ◽  
Sister Chromatid ◽  
Sister Chromatid Exchanges ◽  
Meristematic Cells ◽  
The Third ◽  
Allium Cepa L ◽  
Chromatid Exchanges

ABSTRACT Sister-chromatid exchanges (SCE) were studied in Allium cepa L. meristematic cells at the second and third divisions after BrdUrd-substitution during just the first or during the second and third cycles, respectively. The observed SCE nonreciprocal/reciprocal ratios detected at the third division in both experiments, as well as comparison of the lowest SCE frequency observed per cycle and expressed per picogram of DNA with data from different species expressed accordingly, strongly suggest that most of the exchanges detected in BrdUrd-substituted chromosomes are BrdUrd-dependent events. Hypotheses suggesting some different mechanisms are discussed to explain the formation of these BrdUrd-dependent SCEs.

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