scholarly journals Sensitivity of Fomes annosus Fr. Cooke and Schizophyllum commune Fr. to air pollution with sulphur dioxide

2015 ◽  
Vol 42 (3) ◽  
pp. 347-360 ◽  
Author(s):  
Andrzej Grzywacz
Keyword(s):  
Amino Acids ◽  
Air Pollution ◽  
Nitrogen Content ◽  
Sulphur Dioxide ◽  
Physiological Activity ◽  
Schizophyllum Commune ◽  
Dry Weight ◽  
Mycelium Growth ◽  
Mycelial Mass

The sensivity of mycelium to sulphur dioxide polluting the air depends on its physiological activity. As measure of activity growth was adopted, in the sense of the function of dry weight and diametre of the colony, intensity of respiration, amino acids and nitrogen content in dry mycelial mass. The influence of SO<sub>2</sub> on mycelium growth in dependence on its age and pH of substrate was also investigated.

Amino Acid Composition of Representatives of Eight Bacterial Genera with Reference to Aquatic Productivity

1963 ◽  
Vol 20 (3) ◽  
pp. 729-734 ◽  
Author(s):  
James E. Stewart ◽  
P. L. Hoogland ◽  
H. C. Freeman ◽  
A. E. J. Waddell
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Nitrogen Content ◽  
Acid Composition ◽  
Dry Weight ◽  
Chemical Methods ◽  
Aquatic Productivity

Representatives of eight bacterial genera were grown under identical conditions and the harvested, washed cells were analyzed quantitatively for eighteen amino acids using chemical methods. All of the bacteria contained the eighteen amino acids and, although quantitative differences were noted, variation in composition was not great. Cell nitrogen content averaged 11.91% of the dry weight. Some of the nutritional implications of the data are discussed.

scholarly journals Incorporation of alternative amino acids into cyanophycin by different cyanophycin synthetases heterologously expressed in Corynebacterium glutamicum

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ramona Wördemann ◽  
Lars Wiefel ◽  
Volker F. Wendisch ◽  
Alexander Steinbüchel
Keyword(s):  
Amino Acids ◽  
Dry Weight ◽  
Growth Conditions ◽  
Amino Acid Biosynthesis ◽  
Water Soluble ◽  
Lysine Content ◽  
Polyaspartic Acid ◽  
Acid Biosynthesis ◽  
Cyanophycin Synthetase ◽  
Potential Precursor

AbstractCyanophycin (multi-l-arginyl-poly-l-aspartic acid; also known as cyanophycin grana peptide [CGP]) is a biopolymer that could be used in various fields, for example, as a potential precursor for the synthesis of polyaspartic acid or for the production of CGP-derived dipeptides. To extend the applications of this polymer, it is therefore of interest to synthesize CGP with different compositions. A recent re-evaluation of the CGP synthesis in C. glutamicum has shown that C. glutamicum is a potentially interesting microorganism for CGP synthesis with a high content of alternative amino acids. This study shows that the amount of alternative amino acids can be increased by using mutants of C. glutamicum with altered amino acid biosynthesis. With the DM1729 mutant, the lysine content in the polymer could be increased up to 33.5 mol%. Furthermore, an ornithine content of up to 12.6 mol% was achieved with ORN2(Pgdh4). How much water-soluble or insoluble CGP is synthesized is strongly related to the used cyanophycin synthetase. CphADh synthesizes soluble CGP exclusively. However, soluble CGP could also be isolated from cells expressing CphA6308Δ1 or CphA6308Δ1_C595S in addition to insoluble CGP in all examined strains. The point mutation in CphA6308Δ1_C595S partially resulted in a higher lysine content. In addition, the CGP content could be increased to 36% of the cell dry weight under optimizing growth conditions in C. glutamicum ATCC13032. All known alternative major amino acids for CGP synthesis (lysine, ornithine, citrulline, and glutamic acid) could be incorporated into CGP in C. glutamicum.

scholarly journals Application of Bio-Friendly Formulations of Chitinase-Producing Streptomyces cellulosae Actino 48 for Controlling Peanut Soil-Borne Diseases Caused by Sclerotium rolfsii

2021 ◽  
Vol 7 (3) ◽  
pp. 167
Author(s):  
Gaber Abo-Zaid ◽  
Ahmed Abdelkhalek ◽  
Saleh Matar ◽  
Mai Darwish ◽  
Muhammad Abdel-Gayed
Keyword(s):  
Open Field ◽  
Biocontrol Agent ◽  
Sclerotium Rolfsii ◽  
Dry Weight ◽  
Culture Broth ◽  
Field Conditions ◽  
Damping Off ◽  
Mycelium Growth ◽  
Particle Size Analyzer ◽  
Cell Pellet

Of ten actinobacterial isolates, Streptomyces cellulosae Actino 48 exhibited the strongest suppression of Sclerotium rolfsii mycelium growth and the highest chitinase enzyme production (49.2 U L−1 min−1). The interaction between Actino 48 and S. rolfsii was studied by scanning electron microscope (SEM), which revealed many abnormalities, malformations, and injuries of the hypha, with large loss of S. rolfsii mycelia density and mass. Three talc-based formulations with culture broth, cell-free supernatant, and cell pellet suspension of chitinase-producing Actino 48 were characterized using SEM, Fourier transform infrared spectroscopy (FTIR), and a particle size analyzer. All formulations were evaluated as biocontrol agents for reducing damping-off, root rot, and pods rot diseases of peanut caused by S. rolfsii under greenhouse and open-field conditions. The talc-based culture broth formulation was the most effective soil treatment, which decreased the percentage of peanut diseases under greenhouse and open-field conditions during two successive seasons. The culture broth formulation showed the highest increase in the dry weight of peanut shoots, root systems, and yielded pods. The transcriptional levels of three defense-related genes (PR-1, PR-3, and POD) were elevated in the culture broth formulation treatment compared with other formulations. Subsequently, the bio-friendly talc-based culture broth formulation of chitinase-producing Actino 48 could potentially be used as a biocontrol agent for controlling peanut soil-borne diseases caused by S. rolfsii.

Air Quality Profile in an Enclosed Car Park

2014 ◽  
Vol 567 ◽  
pp. 3-7 ◽  
Author(s):  
Nurul Izma Mohammed ◽  
Nurfadhilah Othman ◽  
Khairul Bariyah Baharuddin
Keyword(s):  
Air Pollution ◽  
Air Quality ◽  
Health Effects ◽  
Sulphur Dioxide ◽  
Motor Vehicle ◽  
Ambient Air ◽  
Ambient Air Pollution ◽  
Kuala Lumpur ◽  
Short Supply ◽  
Car Park

Complaints on poor air quality in an enclosed car park have been raised up among the public, which might cause serious health effects to the drivers, passengers, and labours who are working at the premises. Improper design of mechanical ventilation systems in a car park would result in a poor indoor environment. The exhaust emission of motor vehicle contains a variety of potentially harmful substances encompassing carbon monoxide, nitrogen oxides, sulphur dioxide, hydrocarbons, and fine particulates. In Kuala Lumpur, there is a great demand but a short supply of lands and building spaces. Thus, a large multi-storey underground car parks is a common solution for both, the government and developers. Although the health effects of the motor vehicle emissions and ambient air pollution are already known, but due to the nature of enclosed multi-storey car parks, these health risks are predicted to be intensified. Thus, it is crucial to investigate and evaluate the status of the air pollution in the enclosed car parks with emphasis on sulphur dioxide (SO2) and nitrogen dioxides (NO2). Samples were collected in one of the famous shopping malls in Kuala Lumpur using a GrayWolf Advanced Sense Direct Sense; Toxic Gas Test Meters from 8 am until 5 pm on weekdays and weekends. The results demonstrate that the concentrations of SO2 and NO2 on weekends is higher than weekdays. Besides, the concentrations for both weekdays and weekends have exceeded the standard limit set by the Malaysian Ambient Air Quality Guideline (MAAQG).

scholarly journals Rhizobacteria inoculation in maize associated with nitrogen and zinc fertilization at sowing

2021 ◽  
Vol 25 (2) ◽  
pp. 96-100
Author(s):  
Andréia de L. Moreno ◽  
Jorge F. Kusdra ◽  
Angelita A. C. Picazevicz
Keyword(s):  
Bacillus Subtilis ◽  
Nitrogen Content ◽  
Plant Height ◽  
Nitrogen Fertilization ◽  
Azospirillum Brasilense ◽  
Dry Weight ◽  
Stem Diameter ◽  
Wide Range ◽  
Maize Plants ◽  
Total Dry Weight

ABSTRACT Rhizobacteria have a wide range of plant growth-promoting mechanisms of action, making them an alternative and/or complementary biological input for chemical fertilizers. In this respect, the present study aimed to assess growth and nitrogen accumulation in maize plants as a function of Azospirillum brasilense, Bacillus subtilis, zinc and nitrogen application at sowing. The experiment with the ‘AL Bandeirante’ maize variety was conducted in a greenhouse, using a completely randomized design. The treatments were arranged in a 2 x 2 x 2 x 2 factorial scheme, with six repetitions, considering the presence and absence of Azospirillum brasilense (5 g kg-1), Bacillus subtilis (5 mL kg-1) and zinc (20 g kg-1) in the seeds and addition or not of nitrogen (30 kg ha-1) to the soil. The variables assessed were plant height, stem diameter, leaf, stem, shoot, root and total dry weight, and shoot nitrogen content. Nitrogen fertilization in the absence of zinc increased shoot and total dry weight as well as shoot nitrogen content. Zinc applied to the seeds improved the total and stem dry weight of maize plants in the absence of Bacillus subtilis. Inoculation with Azospirillum brasilense and Bacillus subtilis increased the stem diameter and shoot nitrogen content of maize plants when nitrogen fertilization was not performed at sowing. There was no isolated or interaction effect between factors for plant height and root dry weight.

scholarly journals AMINO ACID COMPOSITION OF LEAVES OF THREE SPECIES OF ARTEMISIA L. GROWING IN THE ELTON REGION

2021 ◽  
pp. 219-225
Author(s):  
Галина Табаленкова
Keyword(s):  
Amino Acids ◽  
Czech Republic ◽  
Amino Acid ◽  
Dry Weight ◽  
Biologically Active ◽  
Raw Material ◽  
Buffer System ◽  
Amino Acid Analyzer ◽  
Growing Conditions ◽  
High Level

The results of studies of the qualitative composition and quantitative content of amino acids (AAs) in the leaves of three plant species of the genus Artemisia, widespread in the Elton region, were presented. Protein AAs were determined on an AAA T-339 amino acid analyzer (Czech Republic) after hydrolysis of a sample in 6N HCl at 105 °C for 24 h, free AAs – on an AAA-400 amino acid analyzer (Czech Republic) in a lithium buffer system. The protein AAs amount varied from 66 mg / g in A. lerchiana to 113 mg / g dry weight in A. santonica. 17 AAs were found in composition of these species, aspartic and glutamic acids were dominant. The content of free AAs varied from 4.4 mg / g in A. santonica to 8.3 mg / g dry weight in A. pauciflora. 14 AAs have been identified, among them proline was the predominant free AA. The share of proline was 75-81% of the total free AAs. Among the minor components, 3-4 compounds with a content above 2% dominated. The free AAs contain 3 non-proteinogenic ones (ornithine, citruline, and γ-aminobutyric acid). A. lerchiana and A. pauciflora species were similar in protein and free amino acids, probably due to the same growing conditions. A high level of free proline, together with a complex of biologically active substances in Artemisia species, which grow abundantly in the Elton region, allow to consider the possibility of their use as a medicinal raw material.

scholarly journals Nitrogenous Excretion by Embryos of the Viviparous Snake Thamnophis S. Sirtalis (L.)

1956 ◽  
Vol 33 (2) ◽  
pp. 384-393 ◽  
Author(s):  
HUGH CLARK ◽  
BETTY FLORIO SISKEN
Keyword(s):  
Amino Acids ◽  
Uric Acid ◽  
Dry Weight ◽  
Garter Snake ◽  
Principal Source ◽  
Wet Weight

1. The garter snake embryo excretes an estimated 2.52 mg. nitrogen, of which 1.4 mg. is recoverable from the embryonic confines. The recovered excreta consist of 16.3% uric acid, 23.4% ammonia and 60.3% urea. 2. The placenta is believed, therefore, to transmit to the mother 1.11 mg. nitrogen per embryo, and it is estimated that it transmits to the embryo approximately 45 mg. of protein as amino-acids. 3. Evidence is presented which suggests that protein may be a principal source of energy, particularly early in development. 4. Growth in terms of wet weight, dry weight, and protein is described.

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