scholarly journals Nitrogenous Excretion by Embryos of the Viviparous Snake Thamnophis S. Sirtalis (L.)

1956 ◽  
Vol 33 (2) ◽  
pp. 384-393 ◽  
Author(s):  
HUGH CLARK ◽  
BETTY FLORIO SISKEN
Keyword(s):  
Amino Acids ◽  
Uric Acid ◽  
Dry Weight ◽  
Garter Snake ◽  
Principal Source ◽  
Wet Weight

1. The garter snake embryo excretes an estimated 2.52 mg. nitrogen, of which 1.4 mg. is recoverable from the embryonic confines. The recovered excreta consist of 16.3% uric acid, 23.4% ammonia and 60.3% urea. 2. The placenta is believed, therefore, to transmit to the mother 1.11 mg. nitrogen per embryo, and it is estimated that it transmits to the embryo approximately 45 mg. of protein as amino-acids. 3. Evidence is presented which suggests that protein may be a principal source of energy, particularly early in development. 4. Growth in terms of wet weight, dry weight, and protein is described.

Growth of fetal guinea pig: physical and chemical characteristics

1985 ◽  
Vol 248 (1) ◽  
pp. E132-E139
Author(s):  
J. W. Sparks ◽  
J. R. Girard ◽  
S. Callikan ◽  
F. C. Battaglia
Keyword(s):  
Amino Acids ◽  
Guinea Pig ◽  
Gestational Age ◽  
Dry Weight ◽  
Carbon And Nitrogen ◽  
Wet Weight ◽  
Near Term ◽  
Total Body ◽  
Physical And Chemical ◽  
Nitrogen Contents

The growth of the fetal guinea pig was studied in 32 fetuses in 12 litters, ranging from 39 days gestation to full term (67 days). The wet weight of the fetus was well approximated by an exponential function of gestational age (GA) in days [fetal weight (g) = 0.993 e(0.068 X GA), r = 0.94]. Dry weight increased more rapidly than wet weight [dry weight (g) = 0.039 e(0.102 X GA); r = 0.97], resulting in an increase in percent dry weight from approximately 10% at 40 days gestation to 30% at term. Fat content increased even more rapidly than dry weight [body fat (g) = 0.00123 e(0.136 X GA), r = 0.97], accounting for 33% of dry weight and 11.7% of wet weight at term. Using bomb calorimetric projections of caloric value of 9.3 kcal X g fat-1 X day-1 and 4.6 kcal X g nonfat dry wt-1 X day-1, we estimate that growth of the fetal guinea pig requires 220 kcal X kg fetal wt-1 X day-1 near term. Carbon and nitrogen contents of the fetus increased at different rates, reflecting the changes in fat and nonfat tissues. Amino acids contributed 80% of total body nitrogen and 41% of total body carbon near term. Cysteine concentrations increased and lysine concentrations decreased with gestational age; the concentrations of the other measured amino acids did not change with gestational age. These studies represent the first systematic study of the chemical growth of the fetus in a nonhuman species.

scholarly journals DEVELOPMENT OF THE METANEPHRIC KIDNEY

1968 ◽  
Vol 37 (3) ◽  
pp. 703-715 ◽  
Author(s):  
G. C. Priestley ◽  
R. A. Malt
Keyword(s):  
Amino Acids ◽  
Kidney Development ◽  
Dry Weight ◽  
Synthetic Activity ◽  
Sharp Change ◽  
Dna Concentration ◽  
Adult Kidney ◽  
Metanephric Kidney ◽  
Wet Weight

The metanephric kidney was studied in fetal and older mice beginning at 16 days after mating of the parents. Polyribosomes from fetal kidneys labeled in vitro with 14C-labeled amino acids had 10–20 times more acid-precipitable radioactivity associated with them than polysomes from adult kidneys similarly labeled. Between 3 and 6 days after birth the rate incorporation of labeled amino acids by polyribosomes from neonatal kidneys declined sharply to only twice the value found for adult kidneys. There was no change in the shape of the polyribosome profile with increasing age, but before birth few, if any, ribosomes were bound to membranes compared with 20% 2 days after birth and between 20 and 30% in the adult. Total protein represented less than 10% of the wet weight in the fetal kidney but increased to 17% of the wet weight in the adult kidney. There was a steady decline in the concentration of RNA and DNA with respect to dry weight throughout kidney development. DNA concentration declined more rapidly than RNA concentration, so that the milligram to milligram ratio of RNA to DNA increased. In males the RNA/DNA ratio was stable at 1.3 at 40 days after birth; but in females the decline in DNA concentration was more protracted, and at 200 days after birth the RNA/DNA ratio was only 0.99. Thus, total nucleic acids show only gradual changes in concentration throughout development of the kidney, but a sharp change in the synthetic activity of the ribosomes and in their binding to membranes occurs in kidneys soon after birth.

BIOASSAY OF PROLACTIN

1969 ◽  
Vol 60 (1) ◽  
pp. 91-100 ◽  
Author(s):  
Charles S. Nicoll
Keyword(s):  
Epithelial Cells ◽  
Lipid Content ◽  
Dry Weight ◽  
Fat Content ◽  
Assay Sensitivity ◽  
Mucosal Epithelium ◽  
Wet Weight ◽  
Total Dry Weight

ABSTRACT The response of the pigeon crop-sac to systemically acting prolactin (injected subcutaneously) was evaluated by measuring the wet weight of the responsive lateral lobes of the organ and by determining the dry weight of a 4 cm diameter disc of mucosal epithelium taken from one hemicrop. Of several different injection schedules tested, administration of prolactin in four daily injections was found to yield optimal responses. When compared with a graded series of prolactin doses, measurement of the mucosal dry weight proved to be a better method of response quantification than determination of the crop-sac wet weight with respect to both assay sensitivity and precision. The submucosal tissue of the crop-sac was estimated to constitute about 64 % of the total dry weight of the unstimulated organ and it was found to be relatively unresponsive to prolactin stimulation in comparison with the mucosa. The lipid content of the mucosal epithelium was determined using unstimulated crop-sacs or tissues which showed varying degrees of prolactin-induced proliferation. The fat content of the mucosal epithelial cells increased only slightly more rapidly than the dry weight or the defatted dry weight of the mucosa. Suggestions are made for the further improvement of the systemic crop-sac assay for prolactin.

Effect of rhizobacteria strains on the induction of resistance in barley genotypes against Cochliobolus sativus

2020 ◽  
Vol 13 (2) ◽  
pp. 83-92 ◽  
Author(s):  
A. Adam
Keyword(s):  
Plant Growth ◽  
Pseudomonas Putida ◽  
Growth Parameters ◽  
Disease Incidence ◽  
Dry Weight ◽  
Cochliobolus Sativus ◽  
Resistance Level ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Barley Genotypes

SummaryEnhancement of the resistance level in plants by rhizobacteria has been proven in several pathosystems. This study investigated the ability of four rhizobacteria strains (Pseudomonas putida BTP1 and Bacillus subtilis Bs2500, Bs2504 and Bs2508) to promote the growth in three barley genotypes and protect them against Cochliobolus sativus. Our results demonstrated that all tested rhizobacteria strains had a protective effect on barley genotypes Arabi Abiad, Banteng and WI2291. However, P. putida BTP1 and B. subtilis Bs2508 strains were the most effective as they reduced disease incidence by 53 and 38% (mean effect), respectively. On the other hand, there were significant differences among the rhizobacteria-treated genotypes on plant growth parameters, such as wet weight, dry weight, plant height and number of leaves. Pseudomonas putida BTP1 strain was the most effective as it significantly increased plant growth by 15-32%. In addition, the susceptible genotypes Arabi Abiad and WI2291 were the most responsive to rhizobacteria. This means that these genotypes have a high potential for increase of their resistance against the pathogen and enhancement of plant growth after the application of rhizobacteria. Consequently, barley seed treatment with the tested rhizobacteria could be considered as an effective biocontrol method against C. sativus.

Respon Akar Bibit Aren Genjah (Arenga pinnata) Di Pembibitan Pada Pemberian Dosis dan Interval Pupuk Organik Cair Nasa

2018 ◽  
Vol 7 (1) ◽  
pp. 28
Author(s):  
Yetti Elidar
Keyword(s):  
Organic Fertilizer ◽  
Dry Weight ◽  
Treatment Combination ◽  
Root Dry Weight ◽  
Randomized Design ◽  
Dose Study ◽  
Palm Sugar ◽  
Wet Weight ◽  
Completely Randomized Design ◽  
Leaf Dry Weight

Research on the response of roots of palm sugar palm seeds (Arenga pinnata) in nurseries at doses and intervals of Nasa liquid organic fertilizer. Aims to determine the dosage, interval and combination of dosages and fertilization intervals with Nasa liquid organic fertilizer which can provide the best dry weight of the roots in the nursery. The research design used was a Completely Randomized Design (CRD) with 3x3 factorial experiments and each treatment was repeated 8 (eight) times, consisting of: the first factor was the treatment of POC Nasa dose in a concentration of 3 cc POC Nasa per liter of water (D) consists of 3 levels, namely: d1 = 300 ml POC Nasa, d2 = 400 ml POC Nasa, d3 = 500 ml POC Nasa, while the second factor is the treatment of POC Nasa Interval (I) consisting of 3 levels, namely: i1 = 2 once a week, i2 = once every 3 weeks, i3 = once every 4 weeks. The results of the POC Nasa dose study had a significant effect on leaf wet weight, leaf dry weight, root wet weight and root dry weight. The best dose at this level are: d2 (400 ml of Nasa liquid organic fertilizer), the interval of liquid organic fertilizer Nasa has a significant effect on leaf wet weight, leaf dry weight, root wet weight and root dry weight. The best dose at this level is: i1 (once every 2 weeks). The treatment combination has no significant effect on all parameters. 

PHYTOTOXICITY OF WASTEWATER CONTAINING CADMIUM (Cd) USING OLEANDER

2020 ◽  
Vol 51 (4) ◽  
pp. 1231-1238
Author(s):  
Zeki & Ridha
Keyword(s):  
Translocation Factor ◽  
Preliminary Test ◽  
Dry Weight ◽  
Exposure Dose ◽  
Test Duration ◽  
Plant Analysis ◽  
Wet Weight ◽  
Simulated Wastewater ◽  
Almost All ◽  
Plant Shoots

This study was aimed to investigate the ability of N.oleander to remove Cadmium (Cd) from wastewater. A prolonged toxicity test was performed in a single exposure and run for 65 days with various concentrations of Cd. Plants were grown in sand medium and irrigated with simulated wastewater contaminated with Cd, using different concentrations (0, 10, 25, 50, 75 mg/L), which were chosen based on previous preliminary test. The results of physical observation of the plants didn’t show any withering symptoms. The Cd concentration in plants increased, while in water decreased. The results of plant analysis showed that Cd concentration in plant shoots (stems and leaves) was higher than that in roots for almost all exposure doses along the test duration. The concentration of Cd in water decreased significantly from the first week of the test and become (0 mg/l) on day-35 for 10 and 25 mg/l exposure doses, while exceeded the permissible limits for 50 and 75 mg/l exposure doses and were 0.14 and 0.91 mg/l, respectively. Wet weight and dry weight of Oleander decreased with increasing Cd concentration level except for 10 mg/l exposure dose where the plant wet weight and dry weight increased at the end of the test. Bioaccumulation factor (BAF) and Translocation Factor (TF) was found to be greater than 1, indicating that Oleander is a successful hyperaccumulator for Cd.

Chemical Fingerprint Analysis and Simultaneous Determination of Nucleosides and Amino Acids in Kang Fu Xin Liquid by High Performance Liquid Chromatography with Diode Array Detector

2020 ◽  
Vol 16 (7) ◽  
pp. 831-843
Author(s):  
Yuwen Wang ◽  
Shuping Li ◽  
Liuhong Zhang ◽  
Shenglan Qi ◽  
Huida Guan ◽  
...  
Keyword(s):  
Amino Acids ◽  
Quality Control ◽  
Uric Acid ◽  
Control Method ◽  
Principal Component ◽  
Gradient Elution ◽  
Hplc Method ◽  
Array Detector ◽  
Fingerprint Analysis ◽  
Wavelength Switching

Background and Objective: Kang Fu Xin liquid (KFX) is an official preparation made from the ethanol extract product from P. Americana. The present quality control method cannot control the quality of the preparation well. The aim of the present study is to establish a convenient HPLC method for multicomponents determination combined with fingerprint analysis for quality control of KFX. Methods: An HPLC-DAD method with gradient elution and detective wavelength switching program was developed to establish HPLC fingerprints of KFX, and 38 batches of KFX were compared and evaluated by similarity analysis (SA), hierarchical clustering analysis (HCA), and principal component analysis (PCA). Meanwhile, six nucleosides and three amino acids, including uracil, hypoxanthine, uric acid, adenosine, xanthine, inosine, tyrosine, phenylalanine and tryptophan in KFX were determined based on the HPLC fingerprints. Results: An HPLC method assisted with gradient elution and wavelength switching program was established and validated for multicomponents determination combined with fingerprint analysis of KFX. The results demonstrated that the similarity values of the KFX samples were more than 0.845. PCA indicated that peaks 4 (hypoxanthine), 7 (xanthine), 9 (tyrosine), 11, 13 and 17 might be the characteristic contributed components. The nine constituents in KFX, uracil, hypoxanthine, uric acid, adenosine, xanthine, inosine, tyrosine, phenylalanine and tryptophan, showed good regression (R2 > 0.9997) within test ranges and the recoveries of the method for all analytes were in the range from 96.74 to 104.24%. The limits of detections and quantifications for nine constituents in DAD were less than 0.22 and 0.43 μg•mL-1, respectively. Conclusion: The qualitative analysis of chemical fingerprints and the quantitative analysis of multiple indicators provide a powerful and rational way to control the KFX quality for pharmaceutical companies.

scholarly journals Incorporation of alternative amino acids into cyanophycin by different cyanophycin synthetases heterologously expressed in Corynebacterium glutamicum

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ramona Wördemann ◽  
Lars Wiefel ◽  
Volker F. Wendisch ◽  
Alexander Steinbüchel
Keyword(s):  
Amino Acids ◽  
Dry Weight ◽  
Growth Conditions ◽  
Amino Acid Biosynthesis ◽  
Water Soluble ◽  
Lysine Content ◽  
Polyaspartic Acid ◽  
Acid Biosynthesis ◽  
Cyanophycin Synthetase ◽  
Potential Precursor

AbstractCyanophycin (multi-l-arginyl-poly-l-aspartic acid; also known as cyanophycin grana peptide [CGP]) is a biopolymer that could be used in various fields, for example, as a potential precursor for the synthesis of polyaspartic acid or for the production of CGP-derived dipeptides. To extend the applications of this polymer, it is therefore of interest to synthesize CGP with different compositions. A recent re-evaluation of the CGP synthesis in C. glutamicum has shown that C. glutamicum is a potentially interesting microorganism for CGP synthesis with a high content of alternative amino acids. This study shows that the amount of alternative amino acids can be increased by using mutants of C. glutamicum with altered amino acid biosynthesis. With the DM1729 mutant, the lysine content in the polymer could be increased up to 33.5 mol%. Furthermore, an ornithine content of up to 12.6 mol% was achieved with ORN2(Pgdh4). How much water-soluble or insoluble CGP is synthesized is strongly related to the used cyanophycin synthetase. CphADh synthesizes soluble CGP exclusively. However, soluble CGP could also be isolated from cells expressing CphA6308Δ1 or CphA6308Δ1_C595S in addition to insoluble CGP in all examined strains. The point mutation in CphA6308Δ1_C595S partially resulted in a higher lysine content. In addition, the CGP content could be increased to 36% of the cell dry weight under optimizing growth conditions in C. glutamicum ATCC13032. All known alternative major amino acids for CGP synthesis (lysine, ornithine, citrulline, and glutamic acid) could be incorporated into CGP in C. glutamicum.

Dehydration of seabird prey during transport to the colony: effects on wet weight energy densities

1987 ◽  
Vol 65 (11) ◽  
pp. 2822-2824 ◽  
Author(s):  
W. A. Montevecchi ◽  
J. F. Piatt
Keyword(s):  
Water Content ◽  
Dry Weight ◽  
Data Sets ◽  
Fresh Weight ◽  
Present Evidence ◽  
Wet Weight ◽  
Cautious Interpretation ◽  
Existing Data

We present evidence to indicate that dehydration of prey transported by seabirds from capture sites at sea to chicks at colonies inflates estimates of wet weight energy densities. These findings and a comparison of wet and dry weight energy densities reported in the literature emphasize the importance of (i) accurate measurement of the fresh weight and water content of prey, (ii) use of dry weight energy densities in comparisons among species, seasons, and regions, and (iii) cautious interpretation and extrapolation of existing data sets.

scholarly journals THE MOLECULAR WEIGHT OF RHODOPSIN AND THE NATURE OF THE RHODOPSIN-DIGITONIN COMPLEX

1954 ◽  
Vol 37 (3) ◽  
pp. 381-399 ◽  
Author(s):  
Ruth Hubbard
Keyword(s):  
Molecular Weight ◽  
Single Molecule ◽  
Extinction Coefficient ◽  
Dry Weight ◽  
Weight Basis ◽  
Sedimentation Behavior ◽  
A Value ◽  
Wet Weight ◽  
Single Boundary ◽  
Stoichiometric Complex

The sedimentation behavior of aqueous solutions of digitonin and of cattle rhodopsin in digitonin has been examined in the ultracentrifuge. In confirmation of earlier work, digitonin was found to sediment as a micelle (D-1) with an s20 of about 6.35 Svedberg units, and containing at least 60 molecules. The rhodopsin solutions sediment as a stoichiometric complex of rhodopsin with digitonin (RD-1) with an s20 of about 9.77 Svedberg units. The s20 of the RD-1 micelle is constant between pH 6.3 and 9.6, and in the presence of excess digitonin. RD-1 travels as a single boundary also in the electrophoresis apparatus at pH 8.5, and on filter paper at pH 8.0. The molecular weight of the RD-1 micelle lies between 260,000 and 290,000. Of this, only about 40,000 gm. are due to rhodopsin; the rest is digitonin (180 to 200 moles). Comparison of the relative concentrations of RD-1 and retinene in solutions of rhodopsin-digitonin shows that RD-1 contains only one retinene equivalent. It can therefore contain only one molecule of rhodopsin with a molecular weight of about 40,000. Cattle rhodopsin therefore contains only one chromophore consisting of a single molecule of retinene. It is likely that frog rhodopsin has a similar molecular weight and also contains only one chromophore per molecule. The molar extinction coefficient of rhodopsin is therefore identical with the extinction coefficient per mole of retinene (40,600 cm.2 per mole) and the E(1 per cent, 1 cm., 500 mµ) has a value of about 10. Rhodopsin constitutes about 14 per cent of the dry weight, and 3.7 per cent of the wet weight of cattle outer limbs. This corresponds to about 4.2 x 106 molecules of rhodopsin per outer limb. The rhodopsin content of frog outer limbs is considerably higher: about 35 per cent of the dry weight, and 10 per cent of the wet weight, corresponding to about 2.1 x 109 molecules per outer limb. Thus the frog outer limb contains about five hundred times as much rhodopsin as the cattle outer limb. But the relative volumes of these structures are such that the ratio of concentrations is only about 2.5 to 1 on a weight basis. Rhodopsin accounts for at least one-fifth of the total protein of the cattle outer limb; for the frog, this value must be higher. The extinction (K500) along its axis is about 0.037 cm.2 for the cattle outer limb, and about 0.50 cm.2 for the frog outer limb.

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