scholarly journals Histochemical demonstration of some hydrolytic enzymes in the spherosomes of plant cells

2015 ◽  
Vol 34 (3) ◽  
pp. 573-588 ◽  
Author(s):  
A. Wałek-Czenecka
Keyword(s):  
Hydrolytic Enzymes ◽  
Plant Cells ◽  
Histochemical Demonstration

Enzyme der Vakuolen aus Wurzelzellen von Maiskeimlingen. Ein Beitrag zur funktionellen Bedeutung der Vakuole bei der intrazellulären Verdauung

1966 ◽  
Vol 21 (9) ◽  
pp. 871-878 ◽  
Author(s):  
Ph. Matile
Keyword(s):  
Cytochrome C ◽  
Alanine Aminotransferase ◽  
Hydrolytic Enzymes ◽  
Plant Cells ◽  
Mitochondrial Enzymes ◽  
Centrifugal Field ◽  
Higher Plant ◽  
Cytochrome C Reductase ◽  
Intracellular Digestion

The isolation of vacuoles from rootlets of corn seedlings based on the slicing and chopping of the plasmolized tissue is described. The isolated vacuoles have densities higher than 1,029 g cm-3; in the centrifugal field they rapidly move centripetally if the extracts are brought to a density of 1,185 g cm-3 by the addition of 30% of “Urografine”. Thus a simple separation of isolated vacuoles from the extracts could be achieved.The following hydrolytic enzymes have been localized in isolated vacuoles: protease, RNase, DNase, phosphatase and two different unspecific esterases. Furthermore two transaminases, aspartate and alanine-aminotransferase and two oxyreductases cytochrome-c-reductase and diaphorase are present in preparations of isolated vacuoles. The absence of mitochondrial enzymes and of enzymes known to be localized in the groundplasm indicated the purity of the preparations of vacuoles.It is concluded that the vacuoles of higher plant cells represent organelles in which the processes of intracellular digestion take place.

Histochemical Demonstration of Some Hydrolytic Enzymes in Atheromatous Aortas

Nature ◽  
1960 ◽  
Vol 188 (4751) ◽  
pp. 677-678 ◽  
Author(s):  
E. LEVONEN ◽  
J. RAEKALLIO ◽  
U. UOTILA
Keyword(s):  
Hydrolytic Enzymes ◽  
Histochemical Demonstration

Olivary hypertrophy: Histochemical demonstration of hydrolytic enzymes

Neurology ◽  
1980 ◽  
Vol 30 (5) ◽  
pp. 471-471 ◽  
Author(s):  
A. H. Koeppen ◽  
K. D. Barron ◽  
M. P. Dentinger
Keyword(s):  
Hydrolytic Enzymes ◽  
Histochemical Demonstration ◽  
Olivary Hypertrophy

scholarly journals Hydrolytic Enzymes in the Central Vacuole of Plant Cells

1979 ◽  
Vol 63 (6) ◽  
pp. 1123-1132 ◽  
Author(s):  
Thomas Boller ◽  
Hans Kende
Keyword(s):  
Hydrolytic Enzymes ◽  
Plant Cells ◽  
Central Vacuole

The diagnosis of metabolic storage disease in skin biopsies (a light-, electronmicroscopic, and analytical study)

1978 ◽  
Vol 36 (2) ◽  
pp. 648-649
Author(s):  
W. Jurecka ◽  
W. Gebhart ◽  
H. Lassmann
Keyword(s):  
Storage Disease ◽  
Hunter Syndrome ◽  
Histochemical Demonstration ◽  
Sweat Glands ◽  
Type I ◽  
Storage Material ◽  
Scheie Syndrome ◽  
Storage Products ◽  
Skin Biopsies ◽  
Type V

Diagnosis of metabolic storage disease can be established by the determination of enzymes or storage material in blood, urine, or several tissues or by clinical parameters. Identification of the accumulated storage products is possible by biochemical analysis of isolated material, by histochemical demonstration in sections, or by ultrastructural demonstration of typical inclusion bodies. In order to determine the significance of such inclusions in human skin biopsies several types of metabolic storage disease were investigated. The following results were obtained.In MPS type I (Pfaundler-Hurler-Syndrome), type II (Hunter-Syndrome), and type V (Ullrich-Scheie-Syndrome) mainly “empty” vacuoles were found in skin fibroblasts, in Schwann cells, keratinocytes and macrophages (Dorfmann and Matalon 1972). In addition, prominent vacuolisation was found in eccrine sweat glands. The storage material could be preserved in part by fixation with cetylpyridiniumchloride and was also present within fibroblasts grown in tissue culture.

SEM study of the morphological effects of kinetin and zeatin on the growth and development of the apical meristem in wheat

1995 ◽  
Vol 53 ◽  
pp. 978-979
Author(s):  
G. M. Hutchins ◽  
J. S. Gardner
Keyword(s):  
Growth And Development ◽  
Furfuryl Alcohol ◽  
Apical Meristem ◽  
Plant Cells ◽  
Triticum Aestivum L ◽  
Morphological Development ◽  
Naturally Occurring ◽  
Chinese Spring Wheat ◽  
Sem Study ◽  
Moist Environment

Cytokinins are plant hormones that play a large and incompletely understood role in the life-cycle of plants. The goal of this study was to determine what roles cytokinins play in the morphological development of wheat. To achieve any real success in altering the development and growth of wheat, the cytokinins must be applied directly to the apical meristem, or spike of the plant. It is in this region that the plant cells are actively undergoing mitosis. Kinetin and Zeatin were the two cytokinins chosen for this experiment. Kinetin is an artificial hormone that was originally extracted from old or heated DNA. Kinetin is easily made from the reaction of adenine and furfuryl alcohol. Zeatin is a naturally occurring hormone found in corn, wheat, and many other plants.Chinese Spring Wheat (Triticum aestivum L.) was used for this experiment. Prior to planting, the seeds were germinated in a moist environment for 72 hours.

Mitochondrial Oxidation of p-N, N-Dimethylamino-β-Phenethylamine (DAPA)

1975 ◽  
Vol 33 ◽  
pp. 458-459
Author(s):  
W. Allen Shannon ◽  
Hannah L. Wasserkrug ◽  
andArnold M. Seligman
Keyword(s):  
Guinea Pig ◽  
Oxidase Activity ◽  
Amine Oxidase ◽  
Histochemical Demonstration ◽  
Guinea Pig Heart ◽  
Pig Kidney ◽  
Cytoplasmic Vacuoles ◽  
Liver And Kidney ◽  
Mitochondrial Oxidation ◽  
Amine Oxidase Activity

The synthesis of a new substrate, p-N,N-dimethylamino-β-phenethylamine (DAPA)3 (Fig. 1) (1,2), and the testing of it as a possible substrate for tissue amine oxidase activity have resulted in the ultracytochemical localization of enzyme oxidase activity referred to as DAPA oxidase (DAPAO). DAPA was designed with the goal of providing an amine that would yield on oxidation a stronger reducing aldehyde than does tryptamine in the histochemical demonstration of monoamine oxidase (MAO) with tetrazolium salts.Ultracytochemical preparations of guinea pig heart, liver and kidney and rat heart and liver were studied. Guinea pig kidney, known to exhibit high levels of MAO, appeared the most reactive of the tissues studied. DAPAO reaction product appears primarily in mitochondrial outer compartments and cristae (Figs. 2-4). Reaction product is also localized in endoplasmic reticulum, cytoplasmic vacuoles and nuclear envelopes (Figs. 2 and 3) and in the sarcoplasmic reticulum of heart.

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