scholarly journals Gasometric method of volume determination of intercellular spaces in plant tissues

2015 ◽  
Vol 33 (2) ◽  
pp. 247-262 ◽  
Author(s):  
J. Czerski
Keyword(s):  
Plant Tissues ◽  
Intercellular Spaces ◽  
Volume Determination

Phosphino-Triazole Ligands for Palladium-Catalysed Cross-Coupling

2018 ◽  
Author(s):  
Yiming Zhao ◽  
Huy van Nguyen ◽  
Louise Male ◽  
Philip Craven ◽  
Benjamin R. Buckley ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Cross Coupling ◽  
Product Formation ◽  
Coupling Reactions ◽  
Volume Determination ◽  
Online Tool ◽  
Triazole Derivatives ◽  
Cross Coupling Reactions ◽  
Modelling Approaches

<div>Twelve 1,5-disubtituted and fourteen 5-substituted 1,2,3-triazole derivatives bearing diaryl or dialkyl phosphines at the 5-position were synthesised and used as ligands for palladium-catalysed Suzuki-Miyaura cross-coupling reactions. Bulky substrates were tested, and lead-like product formation was demonstrated. The online tool SambVca 2.0 was used to assess steric parameters of ligands and preliminary buried volume determination using XRD obtained data in a small number of cases proved to be informative. Two modelling approaches were compared for the determination of</div><div>the buried volume of ligands where XRD data was not available. An approach with imposed steric restrictions was found to be superior in leading to buried volume determinations that closely correlate with observed reaction conversions. The online tool LLAMA was used to determine lead-likeness of potential Suzuki-Miyaura cross-coupling products, from which ten of the most lead-like were successfully synthesised. Thus, confirming these readily accessible triazole-containing phosphines as highly suitable ligands for reaction screening and optimisation in drug discovery campaigns.</div>

scholarly journals An HPLC-automated Derivatization for Glutathione and Related Thiols Analysis in Brassica rapa L.

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1157
Author(s):  
Francesco Nacca ◽  
Concetta Cozzolino ◽  
Petronia Carillo ◽  
Pasqualina Woodrow ◽  
Amodio Fuggi ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
High Performance ◽  
Internal Standard ◽  
Plant Tissues ◽  
Cellular Functions ◽  
Sulphur Compounds ◽  
Stress Protection ◽  
Automated Method ◽  
Reduced Forms

The high content of glucosinolates and glutathione makes the Brassicaceae an important healthy food. Thiols and especially glutathione and γ-Glu-Cys-Gly tripeptide are involved in many fundamental cellular functions such as oxidative stress protection. Although several methods for sulphur compounds analysis in biological samples are actually used, the determination of glutathione and other sulphur derivatives in plant tissues is rather problematic due to their extreme susceptibility to oxidation, which can lead to their overestimation. The aim of this work was the improvement and validation of an automated method for determination of reduced and oxidised glutathione, cysteine and γ-glutamylcysteine in plant tissues. The method consists of a fully automated pre-column derivatization of thiols based on monobromobimane reagent, a high-performance liquid chromatography derivatives separation, and a fluorimetric detection and quantification. The method was successfully applied for determination of the oxidized and reduced forms of Cys, γ-GC and GSH content in leaves, petioles, inflorescences and roots of Brassica rapa L. subsp. Sylvestris. At harvest, in freshly cut plants, the average contents of GSH/2GSSG were 840/45, 345/70 and 150/70 nmol g−1 FW for the florets, leaf blades and stems, respectively; those of Cys/2Cys were 80/12, 29/12 and 24/6 nmol g-1 FW; while those of γ-GC/γ-GCCG-γ were 8.0/4.0, and 6.0/3.0, 3.0/2.0 nmol g−1 FW, respectively. Such amounts were lower in low-sulphur-grown plants at harvest. The very low coefficient of variation between repeated tests (maximum 1.6%), the high recovery of internal standard (>96%) and the linear correlation coefficient of the calibration (R2 > 0.99) support the efficiency of this method that allowed analysing about 50 samples/die in a totally automated manner with no operator intervention. Our results show that the reported method integrations can significantly improve thiols detection via HPLC.

scholarly journals Effects of Macronutrients in the Physiological Quality of Soybean Seeds

2018 ◽  
Vol 10 (5) ◽  
pp. 312
Author(s):  
Geliandro Anhaia Rigo ◽  
Luis Osmar Braga Schuch ◽  
Willian Silva Barros ◽  
Rodrigo Lamaison de Vargas ◽  
Vinícius Jardel Szareski ◽  
...  
Keyword(s):  
Graduate Program ◽  
Plant Tissues ◽  
Soybean Seeds ◽  
Rio Grande Do Sul ◽  
Genetic Characteristics ◽  
Physiological Quality ◽  
High Concentrations ◽  
Nitrogen Phosphorus

The aim of this work was to correlate macronutrient content of soybean seeds to physiological quality of different cultivars. The work was developed in the Federal University of Pelotas, in the facilities of the Seed Science and Technology Graduate Program. The experimental design was randomized blocks in arranged in four replicates. The following soybean cultivars were used: BMX Apolo RR (12 lots), BMX Ativa RR (13 lots), BMX Energia RR (26 lots), BMX Força RR (24 lots), BMX Impacto RR (35 lots), BMX Magna RR (16 lots), BMX Turbo RR (44 lots), BMX Potência RR (82 lots) and NA 5909 RR (28 lots), with seeds produced in northwest Rio Grande do Sul. The determination of nutritional contents found in plant tissues of soybean seeds, were measured: Nitrogen (N), Phosphorus (P), Potassium (K), Magnesium (Mg), Calcium (Ca), Sulfur (S). The macronutrient contents of the seeds vary according to genetic characteristics of the cultivars, with higher oscillations of nitrogen, phosphorus, magnesium and sulfur contents present in soybean seeds. Potassium and calcium are defined as the most stable nutrients for the cultivars and seed lots analyzed. High concentrations of nitrogen, phosphorus and calcium are determinants for the physiological quality of soybean seeds.

The systemic nature of the sunflower disease caused by Diaporthe helianthi

1991 ◽  
Vol 69 (7) ◽  
pp. 1552-1556 ◽  
Author(s):  
M. Muntanola-Cvetković ◽  
Jelena Vukojević ◽  
M. Mihaljčević
Keyword(s):  
Plant Diseases ◽  
Leaf Axil ◽  
Plant Tissues ◽  
Conducting System ◽  
Intercellular Spaces ◽  
Histological Studies ◽  
Leaf Petiole ◽  
Diaporthe Helianthi ◽  
Xylem Elements ◽  
Systemic Nature

The systemic nature of the disease of sunflower plants caused by Diaporthe helianthi, the leaf–petiole–stem route of the host invasion by the fungus, and the plant tissues that were successively affected were demonstrated through histological studies. After penetration into the host, the infection hyphae invade the intercellular spaces and terminal veinlets of the lamina and spread toward larger branches of the conducting system, the midrib, and the petiole. Xylem elements are invaded but are affected less by the fungus attack than the phloem and the parenchyma tissues, which disintegrate completely. Hyphae spread through the leaf axil to the tissues of the stem cortex, where pycnidia of the Phomopsis anamorph are initiated from internal masses of mycelium. Key words: systemic plant diseases, sunflower diseases, Diaporthe helianthi, Phomopsis helianthi.

scholarly journals A New Empirical Approach to Calculating Flood Frequency in Ungauged Catchments: A Case Study of the Upper Vistula Basin, Poland

Water ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 601 ◽  
Author(s):  
Dariusz Młyński ◽  
Andrzej Wałęga ◽  
Tomasz Stachura ◽  
Grzegorz Kaczor
Keyword(s):  
Empirical Model ◽  
Catchment Area ◽  
Flood Frequency ◽  
Ungauged Catchments ◽  
Volume Determination ◽  
Statistical Verification ◽  
Observation Series ◽  
Data Estimation

The aim of the work was to develop a new empirical model for calculating the peak annual flows of a given frequency of occurrence (QT) in the ungauged catchments of the upper Vistula basin in Poland. The approach to the regionalization of the catchment and the selection of the optimal form of the empirical model are indicated as a novelty of the proposed research. The research was carried out on the basis of observation series of peak annual flows (Qmax) for 41 catchments. The analysis was performed in the following steps: statistical verification of data; estimation of Qmax flows using kernel density estimation; determination of physiographic and meteorological characteristics affecting the Qmax flow volume; determination of the value of dimensionless quantiles for QT flow calculation in the upper Vistula basin; verification of the determined correlation for the calculation of QT flows in the upper Vistula basin. Based on the research we conducted, we found that the following factors have the greatest impact on the formation of flood flows in the upper Vistula basin: the size of catchment area; the height difference in the catchment area; the density of the river network; the soil imperviousness index; and the volume of normal annual precipitation. The verification procedure that we performed made it possible to conclude that the developed empirical model functions correctly.

Determination of indole-3-acetic acid in plant tissues by capillary electrophoresis

1997 ◽  
Vol 779 (1-2) ◽  
pp. 342-346 ◽  
Author(s):  
M. Brüns ◽  
A. Bazzanella ◽  
H. Lochmann ◽  
K. Bächmann ◽  
C. Ullrich-Eberius
Keyword(s):  
Acetic Acid ◽  
Capillary Electrophoresis ◽  
Plant Tissues ◽  
Indole 3 Acetic Acid
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