scholarly journals Aspergillus giganteus Wehm. mut. alba Zurz.

2015 ◽  
Vol 32 (4) ◽  
pp. 715-718 ◽  
Author(s):  
A. Zurzycka
Keyword(s):  
Aspergillus Giganteus

scholarly journals Purification and Characterization of a Unique Pectin Lyase from Aspergillus giganteus Able to Release Unsaturated Monogalacturonate during Pectin Degradation

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Danielle Biscaro Pedrolli ◽  
Eleonora Cano Carmona
Keyword(s):  
Divalent Cations ◽  
Orange Peel ◽  
Pectin Lyase ◽  
Soluble Product ◽  
Aspergillus Giganteus ◽  
Pectic Substances ◽  
Orange Peel Waste ◽  
Pectin Lyases ◽  
Vegetable Oil Extraction

A pectin lyase, named PLIII, was purified to homogeneity from the culture filtrate of Aspergillus giganteus grown in submerged culture containing orange peel waste as carbon source. PLIII was able to digest apple pectin and citrus pectins with different degrees of methyl esterification. Interestingly, the PLIII activity was stimulated in the presence of some divalent cations including Pb2+ and was not significantly affected by Hg2+. Like other pectin lyases, PLIII is stimulated by but is not dependent on Ca2+. The main soluble product released during the degradation of pectic substances promoted by the PLIII is compatible with an unsaturated monogalacturonate. PLIII is a unique enzyme able to release unsaturated monogalacturonate as the only soluble product during the degradation of pectic substances; therefore, PLIII was classified as an exo-pectin lyase. To our knowledge, this is the first characterization of an exo-pectin lyase. The PLIII described in this work is potentially useful for ethanol production from pectin-rich biomass, besides other common applications for alkaline pectinases like preparation of textile fibers, coffee and tea fermentation, vegetable oil extraction, and the treatment of pulp in papermaking.

scholarly journals Highly Oxygenated Constituents from a Marine Alga-Derived Fungus Aspergillus giganteus NTU967

Marine Drugs ◽  
2020 ◽  
Vol 18 (6) ◽  
pp. 303
Author(s):  
Jih-Jung Chen ◽  
Shih-Wei Wang ◽  
Yin-Ru Chiang ◽  
Ka-Lai Pang ◽  
Yueh-Hsiung Kuo ◽  
...  
Keyword(s):  
Marine Alga ◽  
Ethyl Acetate Extract ◽  
Tube Formation ◽  
Disc Diffusion ◽  
Anticancer Activities ◽  
Preliminary Screening ◽  
Aspergillus Giganteus ◽  
Fermented Broth ◽  
Ic50 Values ◽  
Antimicrobial Assay

Agar-based disc diffusion antimicrobial assay has shown that the ethyl acetate extract of the fermented broth of Aspergillus giganteus NTU967 isolated from Ulva lactuca exhibited significant antimicrobial activity in our preliminary screening of bioactive fungal strains. Therefore, column chromatography of the active principles from liquid- and solid–state fermented products of the fungal strain was carried out, and which had led to isolation of eleven compounds. Their structures were determined by spectral analysis to be seven new highly oxygenated polyketides, namely aspergilsmins A–G (1–7), along with previously reported patulin, deoxytryptoquivaline, tryptoquivaline and quinadoline B. Among these, aspergilsmin C (3) and patulin displayed promising anticancer activities against human hepatocellular carcinoma SK-Hep-1 cells and prostate cancer PC-3 cells with IC50 values between 2.7–7.3 μM. Furthermore, aspergilsmin C (3) and patulin exhibited significant anti-angiogenic functions by impeding cell growth and tube formation of human endothelial progenitor cells without any cytotoxicity.

scholarly journals The Antifungal Protein AFP from Aspergillus giganteus Inhibits Chitin Synthesis in Sensitive Fungi

2007 ◽  
Vol 73 (7) ◽  
pp. 2128-2134 ◽  
Author(s):  
Silke Hagen ◽  
Florentine Marx ◽  
Arthur F. Ram ◽  
Vera Meyer
Keyword(s):  
Cell Wall ◽  
Wall Stress ◽  
Chitin Synthase ◽  
Antifungal Protein ◽  
Cell Integrity ◽  
Class Iii ◽  
Chitin Synthesis ◽  
Aspergillus Giganteus ◽  
Cell Wall Stress ◽  
Sytox Green

ABSTRACT The antifungal protein AFP from Aspergillus giganteus is highly effective in restricting the growth of major human- and plant-pathogenic filamentous fungi. However, a fundamental prerequisite for the use of AFP as an antifungal drug is a complete understanding of its mode of action. In this study, we performed several analyses focusing on the assumption that the chitin biosynthesis of sensitive fungi is targeted by AFP. Here we show that the N-terminal domain of AFP (amino acids 1 to 33) is sufficient for efficient binding of AFP to chitin but is not adequate for inhibition of the growth of sensitive fungi. AFP susceptibility tests and SYTOX Green uptake experiments with class III and class V chitin synthase mutants of Fusarium oxysporum and Aspergillus oryzae showed that deletions made the fungi less sensitive to AFP and its membrane permeabilization effect. In situ chitin synthase activity assays revealed that chitin synthesis is specifically inhibited by AFP in sensitive fungi, indicating that AFP causes cell wall stress and disturbs cell integrity. Further evidence that there was AFP-induced cell wall stress was obtained by using an Aspergillus niger reporter strain in which the cell wall integrity pathway was strongly induced by AFP.

Characterization of the gene encoding α-sarcin, a ribosome-inactivating protein secreted by Aspergillus giganteus

Gene ◽  
1993 ◽  
Vol 124 (2) ◽  
pp. 239-244 ◽  
Author(s):  
S. Wnendt ◽  
H. Felske-Zech ◽  
P.-P.C. Henze ◽  
N. Ulbrich ◽  
U. Stahl
Keyword(s):  
Ribosome Inactivating Protein ◽  
Gene Encoding ◽  
Aspergillus Giganteus

Dissemination of fungi by migratory birds: survival and recovery of fungi from birds

1970 ◽  
Vol 48 (5) ◽  
pp. 907-910 ◽  
Author(s):  
Gloria M. Warner ◽  
D. W. French
Keyword(s):  
Plant Pathogens ◽  
Migratory Birds ◽  
Puccinia Coronata ◽  
Aspergillus Giganteus

Spores of Cladosporium resinae and Aspergillus giganteus applied to feathers of 149 birds of 31 species were recovered in a viable condition from 18 of these birds 3–45 days later. Fungi in 39 genera, including some plant pathogens, were isolated from the feathers of 248 birds collected in Minnesota, Texas, and Mexico. Puccinia coronata and Helminthosporium avenae were transmitted by grackles from infected oat plants in the field to 22% and 52%, respectively, of the greenhouse seedlings which were exposed to the birds.

scholarly journals Activity of the Antifungal Protein from Aspergillus giganteus Against Botrytis cinerea

2003 ◽  
Vol 93 (11) ◽  
pp. 1344-1353 ◽  
Author(s):  
Ana Beatriz Moreno ◽  
Álvaro Martínez del Pozo ◽  
Marisé Borja ◽  
Blanca San Segundo
Keyword(s):  
Botrytis Cinerea ◽  
Ornamental Plants ◽  
Crop Protection ◽  
Gray Mold ◽  
Antifungal Protein ◽  
Low Molecular Weight ◽  
Aspergillus Giganteus ◽  
Cecropin A ◽  
Weight Protein ◽  
Low Molecular Weight Protein

Botrytis blight (gray mold), caused by Botrytis cinerea, is one of the most widely distributed diseases of ornamental plants. In geranium plants, gray mold is responsible for important losses in production. The mold Aspergillus giganteus is known to produce and secrete a basic low-molecular-weight protein, the antifungal protein (AFP). Here, the antifungal properties of the Aspergillus AFP against various B. cinerea isolates obtained from naturally infected geranium plants were investigated. AFP strongly inhibited mycelial growth as well as conidial germination of B. cinerea. Microscopic observations of fungal cultures treated with AFP revealed reduced hyphal elongation and swollen hyphal tips. Washout experiments in which B. cinerea was incubated with AFP for different periods of time and then washed away revealed a fungicidal activity of AFP. Application of AFP on geranium plants protected leaves against Botrytis infection. Cecropin A also was active against this pathogen. An additive effect against the fungus was observed when AFP was combined with cecropin A. These results are discussed in relation to the potential of the afp gene to enhance crop protection against B. cinerea diseases.

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