scholarly journals Occurrence of entomopathogenic fungi in arable soil and meadow soil

2014 ◽  
Vol 27 (2) ◽  
pp. 197-203 ◽  
Author(s):  
Ryszard Miętkowski ◽  
Cezary Tkaczyk ◽  
Ludwik Zasada
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Arable Soil ◽  
Meadow Soil ◽  
Paecilomyces Farinosus ◽  
Arable Field ◽  
Laspeyresia Pomonella

The samples of soil for experiment were taken from arable field and meadow. Larvae of <i>Laspeyresia pomonella</i> and <i>Plodia interpuactella</i> were used as an "insect bait" for isolation of entomopathogenic fungi from the soil. <i>Beauveria bassiana, Metarhizium anisopliae, Paecilomyces farinosus</i> and <i>P. fumosoroseus</i> were isolated from both kinds of soil.

scholarly journals Entomopathogenic fungi isolated in the vicinity of Szczecin

2014 ◽  
Vol 33 (1) ◽  
pp. 123-130 ◽  
Author(s):  
Ryszard Miętkiewicz ◽  
Magdalena Dzięgielewska ◽  
Krystyna Janowicz
Keyword(s):  
Forest Soil ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Dominant Species ◽  
Beech Forest ◽  
Meadow Soil ◽  
Paecilomyces Farinosus ◽  
Opposite Tendency

The spectrum and occurrence of entomopathogenic fungi in litter beech forest. meadow soil and arable soil were evaluated using an "insect bait method". Soil and litter samples taken in autumn and spring from each stand were baited with <i>G. mellonella</i> larvae at 22°C. Six species of entomopathogenic fungi infected these larvae. In the litter <i>Beauveria bassiana</i> was the dominant species. Besides <i>B. bassiana</i>, 23% of <i>G. mellonella</i> larvae were infected by <i>Paecilomyces farinosus</i> in autumn. In the forest soil the dominant entomopathogenic fungus was not found. In this stand the number of infected larvae was the lowest. <i>Metarhizium anisopliae</i> was the dominant species in the meadow soil in both sampling periods. In the soil taken from a rye field<i> P. fumosoroseus</i> and <i>B. bassiana</i> infected <i>G. mellonella</i> larvae almost to the same degree. It was established that <i>B. bassiana</i> and <i>M. anisopliae</i> infected more <i>G. mellonella</i> larvae in spring than in autumn. In the case of <i>P. farinosus</i> and <i>P. fumosoroseus</i> an opposite tendency was observed.

scholarly journals Occurrence of entomopathogenic fungi in arable soil

2014 ◽  
Vol 28 (1) ◽  
pp. 77-82 ◽  
Author(s):  
Ryszard Miętkiewski ◽  
Zofia Miętkiewska
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Galleria Mellonella ◽  
Arable Soil ◽  
Paecilomyces Fumosoroseus ◽  
Arable Field ◽  
Ephestia Kühniella

Samples of soil were taken from arable field and from balk. Larvae of <i>Galleria mellonella</i> and <i>Ephestia kühniella</i> were used as an "insect bait" for isolation of entomopathogenic fungi from soil. <i>Metarhizium anisopliae</i> and <i>Paecilomyces fumosoroseus</i> were isolated from both kind of soil. but <i>Beauveria bassiana</i> was present only in soil taken from balk.

scholarly journals A response of entomopathogenic fungi to botanical fungicides and synthetic fungicides in vitro

2012 ◽  
Vol 58 (1) ◽  
pp. 101-111
Author(s):  
Anna Sapieha-Waszkiewicz ◽  
Barbara Marjańska-Cichoń ◽  
Ryszard Miętkiewski ◽  
Mieczysław Żurek
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Galleria Mellonella ◽  
Morphological Changes ◽  
Fungal Growth ◽  
Apple Orchard ◽  
Paecilomyces Fumosoroseus ◽  
Arable Field

The growth colonies' morphology and sporulation of entomopathogenic fungi was estimated on Sabouraud medium containing botanical fungicides (Bioczos liquid and Biosept 33 SL), and synthetic ones (Teldor 500 SC and Sumilex 500 SC). Entomopathogenic fungi (<i>Beauveria bassiana</i> (Balls.) Vuill., <i>Metarhizium anisopliae</i> (Metsch.) Sorok, and <i>Paecilomyces fumosoroseus</i> (Wize) Brown et Smith.), were isolated from soil by means of <i>Galleria mellonella</i> larvae as baits. Isolates Bb I, Ma I, Pf I derivered from herbicide fallow from apple orchard and isolates Bb II, Ma II and Pf II from arable field adjacent to orchard. Fungicides were added to Sabouraud medium at the following concentrations: A-recommended dose, B-10-times lower than the recommended, C-100-times lower than the recommended. The growth of colonies their morphological changes and sporulations were estimated after 5 and 20 days. The same parametrs were observed when fungi were transfered from the medium containing fungicides on the medium with out fungicides. From botanical fungicides Bioczos liquid inhibited fungal growth more than Biosept 33 SL. Both of fungicides were most toxic to fungi at concentration A. Both of <i>P. fumosoroseus</i> isolates were the most sensitive to Bioczos liquid and <i>M. anisopliae</i> to Biosept 33 SL. All concentrations of synthetic fungicides (Sumilex 500 SC, Teldor 500 SC) restricted growth of <i>B. bassiana</i> and <i>M. anisopliae</i> isolates more from arable soil than from herbicide fallow but opposite reaction was found with respect to <i>P. fumosoroseus</i>.

scholarly journals On the virulence of the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Ascomycota: Hypocreales), against the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Muhammad Shehzad ◽  
Muhammad Tariq ◽  
Tariq Mukhtar ◽  
Asim Gulzar
Keyword(s):  
Sex Ratio ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Insect Pest ◽  
Diamondback Moth ◽  
Economic Damage ◽  
Offspring Sex Ratio ◽  
Development Of Resistance

Abstract Background The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a noxious pest of cruciferous crops all over the world causing serious economic damage. Management of insect pest generally depends on chemical control; however, due to development of resistance against all types of insecticides, alternative approaches especially utilization of a microbial agent is inevitable. Results Potential of 2 entomopathogenic fungi (EPF), viz., Beauveria bassiana and Metarhizium anisopliae, was evaluated against 2nd and 3rd larval instars of P. xylostella by adopting leaf dip and direct spraying methods under laboratory conditions. Significant mortality rate was achieved by each fungus under adopted methodologies. However, B. bassiana was found to be more effective in both conditions than M. anisopliae. Highest mean corrected mortality (77.80%) was recorded, when spores of B. bassiana were sprayed on the 2nd instar larvae (LC50=1.78×104/ml) after the 6th day of treatment. Similarly, incase of M. anisopliae LC50 for the 2nd instar at the same methodology was 2.78×104/ml with a mortality percentage of 70.0%. Offspring sex ratio was non-significantly related to treatment concentration and methodology, except for the control. Conclusion Beauveria bassiana and M. anisopliae had potential to suppress P. xylostella infestations when applied appropriately. Present findings suggested that B. bassiana and M. anisopliae when sprayed on immatures of host insect had more effect as compared to leaf dip procedure. Furthermore, no significant effect of concentrations was observed on sex ratio.

scholarly journals Evaluating the virulence of Metarhizium anisopliae (Deuteromycotina: Hyphomycetes) and Beauveria bassiana (Ascomycota: Hypocreales) isolates to Arabian rhinoceros beetle, Oryctes agamemnon arabicus

2015 ◽  
Vol 47 (3) ◽  
pp. 117 ◽  
Author(s):  
M.W. Khudhair ◽  
M.Z. Khalaf ◽  
H.F. Alrubeai ◽  
A.K. Shbar ◽  
B.S. Hamad ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Pupal Stage ◽  
Control Treatment ◽  
Rhinoceros Beetle ◽  
Oryctes Agamemnon ◽  
Lethal Time ◽  
Short Time

Virulence of entomopathogenic fungi <em>Metarhizium anisopliae</em> and <em>Beauveria bassiana</em> were tested against Arabian Rhinoceros Beetle, <em>Oryctes agamemnon arabicus</em> larvae. Four concentrations (1×10<sup>5</sup>, 1×10<sup>7</sup>, 1×10<sup>9</sup> and 1×10<sup>11</sup> conidia/mL<sup>–1</sup>) of two locally isolated entomopathogenic fungi spore suspensions were used in this study via larval direct spraying. Results revealed that both isolates can cause high mortality rate reaching 100% after 29 days. However, <em>Beauveria bassiana</em> scored higher mortality rate in short time especially at the concentration of 1×10<sup>11</sup> conidia/ mL<sup>–1</sup> with lethal time (LT)<sub>50</sub> 12.75 and LT<sub>90</sub> 20.00; while, <em>Metarhizium anisopliae</em> caused the higher percentage of malformed adults. Moreover, both isolates affected insect’s life cycle particularly in the pupal stage which was reduced remarkably by almost 50% in comparison with the control treatment.

scholarly journals Effect of Biofertilizers and Neem Oil on the Entomopathogenic Fungi Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok

2001 ◽  
Vol 44 (4) ◽  
pp. 419-423 ◽  
Author(s):  
Edson Hirose ◽  
Pedro M. O. J. Neves ◽  
João A. C. Zequi ◽  
Luís H. Martins ◽  
Cristiane H. Peralta ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Azadirachta Indica ◽  
Organic Agriculture ◽  
Metarhizium Anisopliae ◽  
Neem Oil ◽  
Colony Diameter ◽  
Negative Effect ◽  
Fungitoxic Effect

The in vitro fungitoxic effect of three biofertilizers, E.M.-4, Multibion <FONT FACE=Symbol>Ô</FONT> and Supermagro used in organic agriculture and the neem oil (Azadirachta indica A. Juss) on the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana was studied. These products were mixed in a medium where the two fungi were inoculated, and germination, vegetative growth and conidiogenesis were assessed. The biofertilizers Supermagro and E.M.- 4 showed to be less toxic for the two fungi whereas Multibion<FONT FACE=Symbol>Ô</FONT> caused major inhibition on M. anisopliae, with reductions in germination (-37.74%), colony diameter (-30.26%) and conidiogenesis (-42.62%). Neem oil promoted a larger negative effect on B. bassiana, inhibiting germination (-45.27%), colony diameter (-36.62%) and conidiogenesis (-84.93%).

scholarly journals Effectiveness of Selected Entomopathogenic Fungi in Packed Rice Grain at Room Temperature Against Corcyra Cephalonica Stainton

2017 ◽  
Vol 23 (3) ◽  
pp. 183 ◽  
Author(s):  
Hendrawan Samodra ◽  
Yusof Bin Ibrahim
Keyword(s):  
Beauveria Bassiana ◽  
High Mortality ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Room Temperature ◽  
Larval Mortality ◽  
Corcyra Cephalonica ◽  
Rice Grain ◽  
Rice Moth ◽  
Tapioca Flour

Eight isolates of entomopathogenic fungi were evaluated as dried conidia against the rice moth,  Corcyra cephalonica. In bioassays two isolates of Beauveria bassiana (BbGc and BbPs) and one isolate of Metarhizium anisopliae (MaPs) consistently gave high mortality to C. cephalonica larvae. Formulations in either kaolin, talc or tapioca flour (20 % w/w a.i.) thoroughly mixed with long grain rice in plastic cups (8 cm diameter by 5 cm) gave complete larval mortality by the 12th day of treatment. However, in general those formulated in kaolin and talc were more efficacious and faster to kill compared to those formulated in tapioca flour or the unformulated control. Even at the lowest rate of 0.05 g BbGc in kaolin provided 100% mortality 7 days after introduction compared with other dust formulations. Isolate BbGc in kaolin and talc administered at 0.4 g a.i. in 200 g rice packed in plastic kept at room temperature provided protection against the rice moth up to 4 months of storage. Larval mortality in excess of 90% was obtained 15 days after introduction. Formulations of MaPs was effective only within the first month of storage beyond which infectivity rapidly declined.

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