scholarly journals A taxonomic study of o strain of Trichosporon beigelii (Küch. et Rabenh.) Vuillemin from the soil

2014 ◽  
Vol 18 (2) ◽  
pp. 289-296
Author(s):  
Zofaia Maciejowska-Pokacka

The morphology and physiology of a strain of <i>Trichosporon</i> beigelii was studied. Several kinds of spores and conidial stalks were observed. The observations indicate morphological characteristics should be used as positive and negative criteria in Identification of species of <i>Trichosporon</i> and in distinguishing it from related genera.

Plant Disease ◽  
1999 ◽  
Vol 83 (1) ◽  
pp. 62-65 ◽  
Author(s):  
A. De Cal ◽  
P. Melgarejo

The effect of long-wave UV/dark period on mycelial growth of 46 isolates of Monilinia sp. collected in Spain and 16 isolates collected from other parts of the world was investigated. Typical isolates of M. laxa, M. fructigena, and M. fructicola were grown in the dark and identified by morphological characteristics. Long-wave UV/dark conditions reduced the growth rates of M. laxa, M. fructigena, and M. fructicola on potato dextrose agar. All isolates of M. fructigena grew more slowly than those of M. fructicola. Typical and atypical isolates of M. fructigena and M. fructicola were placed in their respective species based on long-wave UV/dark growth rate data. M. laxa isolates were readily distinguished by the short distance from their conidium to the first germ tube branch. The involvement of different photoreceptors in photoresponses by M. fructicola and M. fructigena is discussed. Differences in mycelial growth under long-wave UV may be a useful tool to identify Monilinia spp.


2019 ◽  
Vol 180 (2) ◽  
pp. 95-101 ◽  
Author(s):  
T. Kh. Kumachova ◽  
O. O. Beloshapkina ◽  
A. S. Voronkov ◽  
A. S. Ryabchenko

Background. Resistance to the effects of plant biotic stressors is determined by a set of factors. Among them, the leading protective role is often assigned to the physiological and biochemical characteristics of the surface tissues. However, one cannot ignore the specificity of the microstructural organization of the plant surface, since the nature of interactions in phytopathogenic organisms is more complex than the chemical impact. Meanwhile, the information accumulated to date about the structure of the surface tissues of the vegetative and reproductive organs of plants, and the interface of mycobiota, is fragmentary.Objective. Mature leaves and fruits taken from representatives of the subfamily Maloideae Werber (Malus domestica Borkh., Pyrus communis L., Cydonia oblonga Mill. and Mespilus germanica L.) were selected for the study.Materials and methods. Samples for the research were taken from the middle part of the crown of model trees in 3 replications. In recent years, scanning electron microscopy (SEM) with cryofixation is considered the most promising technique and is used to analyze the surface of biological organisms and identification of species. It is particularly informative in the case of organisms with complex surface micromorphology and for studying the biodiversity of pathogens. However, in this work we combined the methods of light, electron (SEM, TEM) and confocal microscopy. The samples were also studied using phytopathological and histochemical techniques. Condensed polyphenols were detected using K2Cr2O7 and FeCl3 as well as 4-(Dimethylamino)cinnamaldehyde (DMACA, Sigma-Aldrich).Results. On the basis of the obtained phytopathological materials and published data an overview of fungal diseases afflicting leaves and fruits of M. domestica, P. communis, C. oblonga and M. germanica was made. It has been established that a common feature of the Maloideae fruits is the accumulation of condensed polyphenols, which play an important protective role against biotic stressors, in the cells of the pericarp’s outer tissues. Anatomical and morphological characteristics of passive immunity, or horizontal resistance to fungal pathogens, include the specific nature of waxy and cuticular deposits, features of the formation of cuticular folds and peristomatic rings in the stomata area and microstrands at the base of trichomes, thickness of the cuticle and cork tissue, and the development of lenticels on fruits.Conclusion. The studied model plants suffer from a sufficiently wide range of diseases with different etiologies; among them, the most widespread and harmful are mycoses. In view of this, their resistance to fungal pathogens correlates with the specificity of the leaf and fruit surface microstructure and the content of phenolic substances (polyphenols) in the cells of the pericarp’s surface tissues.


2016 ◽  
Vol 21 (2) ◽  
pp. 443-446 ◽  
Author(s):  
Juan Pablo Zanotto Arpellino ◽  
Romina Elizabeth Principe ◽  
Ana Maria Oberto ◽  
Cristina Mabel Gualdoni

<p>La taxonomía de Chironomidae se apoya fuertemente en la integración de las características morfológicas de todas las fases del ciclo de vida, las cuales se pueden obtener mediante la cría en laboratorio. Este estudio desarrolló un método de cría en laboratorio de larvas de Chironomidae de ambientes lóticos. El método consta de una circulación constante de agua y distintos ítems alimenticios. Esta metodología ha permitido obtener asociaciones de los estados de larva, pupa y adulto para la identificación de especies presentes en una región de Argentina en la que el estado de conocimiento de la familia Chironomidae es incipiente.</p><p><strong>Laboratory Rearing Methodology for Chironomidae (Diptera) of Lotic Environments</strong></p><p>The taxonomy of Chironomidae is strongly supported by the integration of morphological characteristics of all stages of the life cycle, which can be obtained through laboratory rearing. This study was developed in laboratory to test rearing method for lotic Chironomidae larvae. The method included constant water circulation and different food items. This methodology allowed obtaining associations of larva, pupa and adult stages for the identification of species from an Argentinean region in which the knowledge of Chironomidae family is incipient.</p>


2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Roberta Lima Caldeira ◽  
Tatiana Maria Teodoro ◽  
Liana Konovaloff Jannotti-Passos ◽  
Pollanah M. Lira-Moreira ◽  
Christiane De Oliveira Goveia ◽  
...  

The identification of snails of the genusBiomphalariacan be done using morphological characteristics which depends on the size of the snails and skill and knowledge of researcher. These methods sometimes are not adequate for identification of species. The PCR-RFLP, using the ITS region of the rDNA, has been used to identify Brazilian species of the genusBiomphalaria. Nevertheless, there is a lack of information about snails from other Latin American countries. In addition, some snails may be infected bySchistosoma mansoniand when submitted to PCR-RFLP they show molecular profiles different from those previously standardized for the other mollusc species. In this work the molecular profiles of 15 species and the subspecies were established by PCR-RFLP of ITS-rDNA with the enzymeDdeI. Moreover, the molecular profiles of host species,B. glabrata,B. straminea,B. tenagophila, andB. prona, infected byS. mansoniwere also established. The molluscs were dissected to permit morphological identification. These results contribute to a correct identification of snails of the genusBiomphalariaand detection of these snails infected byS. mansoni.


2007 ◽  
Vol 57 (7) ◽  
pp. 1640-1646 ◽  
Author(s):  
Marc René Carlsohn ◽  
Ingrid Groth ◽  
Geok Yuan Annie Tan ◽  
Barbara Schütze ◽  
Hans-Peter Saluz ◽  
...  

Three actinomycetes isolated from the surfaces of rocks in a medieval slate mine were examined in a polyphasic taxonomic study. Chemotaxonomic and morphological characteristics of the isolates were typical of strains of the genus Amycolatopsis. The isolates had identical 16S rRNA gene sequences and formed a distinct phyletic line towards the periphery of the Amycolatopsis mediterranei clade, being most closely related to Amycolatopsis rifamycinica. The organisms shared a wide range of genotypic and phenotypic markers that distinguished them from their closest phylogenetic neighbours. On the basis of these results, a novel species, Amycolatopsis saalfeldensis sp. nov., is proposed. The type strain is HKI 0457T (=DSM 44993T=NRRL B-24474T).


1963 ◽  
Vol 9 (4) ◽  
pp. 499-507 ◽  
Author(s):  
Franklin P. Koontz ◽  
John E. Faber

An investigation of 110 cultures of Gram-negative bacteria which failed to produce acid in conventional carbohydrate media was undertaken to determine their taxonomic position.Thirty-seven strains had peritrichous flagella, 30 were monotrichous, 8 were lophotrichous, 3 were either monotrichous or lophotrichous, and 32 strains were non-flagellated.Flagella stains and reaction in Hugh and Leifson"s O/F medium were used to divide the strains into general groups.Sixty-five strains were inert in O/F medium. Forty of these resembled the general description for the genus Alcaligenes. Seventeen strains resembled Alcaligenes biochemically, but were either monotrichously or lophotrichously flagellated. Several strains resembled Vibrio percolans.Nine strains resembled Achromobacter biochemically by oxidatively utilizing pentoses only.Twenty-three strains resembled Pseudomonas aeruginosa. All produced acid oxidatively in the O/F medium.Thirteen strains produced acid fermentatively in O/F medium.Serological studies indicated antigenic heterogeneity. Several strains shared flagellar and somatic antigens, while others were related by either somatic or flagellar components. Seventy-eight strains did not react with any somatic antisera and 54 strains did not react with any flagellar antisera. There was little correlation between antigenicity and biochemical or morphological characteristics.


1991 ◽  
Vol 61 (1) ◽  
pp. 3-15 ◽  
Author(s):  
Janet W. Reid

Collections from the Brazilian Planalto and Amazon regions contained several species of the cyclopoid copepod genus Tropocyclops. The morphology of T. schubarti is discussed; new records of this species extend its known distribution west-ward to the Brazilian Amazon and central highlands. Morphological characteristics of some populations of the T. prasinus-group are most similar to T. prasinus mexicanus and T. prasinus s. str. Tropocyclops federensis n. sp. and T. nananae n. sp. are described from the Distrito Federal. A key and chart are provided for the identification of species of Tropocyclops recorded from the Americas.


2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Zahoor Ahmad Itoo ◽  
Zafar A. Reshi ◽  
Qussin Basharat ◽  
Sheikh Tahir Majeed ◽  
Khurshid Iqbal Andrabi

The coniferous forests of Kashmir Himalayas provide a sustainable habitat for wide varieties of ectomycorrhizal fungi. The identification and characterization of many of these fungi however largely involves morphological descriptions of sporocarps alone, thus sometimes raising questions about the authenticity of these studies. The present study was carried out to identify and characterize ectomycorrhizal fungi from the coniferous forests of Kashmir Himalaya using both morphological and molecular methods. Herein we report on the identification and characterization of three potential ectomycorrhizal Cortinarius fungal species, namely, Cortinarius flexipes (Pers. Ex Fr.), Cortinarius fulvoconicus M. M. Moser, and Cortinarius infractus (Pers.) Fr., from Kashmir Himalaya, India, on the basis of their morphological and molecular characterization. Morphological characteristics of all species were measured and compared with standard taxonomic literature. ITS-rDNA (the fungal molecular marker) was used for molecular analysis. The target region of rDNA (ITS1 5.8s ITS2) of these species was amplified using universal fungal primers (ITS1 and ITS4). The sequencing of amplified products and their subsequent blast analysis confirmed the identification of species by comparing the sequences of these species with respective species sequences present in GenBank. Phylogenetic analysis also confirmed the identification of species.


Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
Felipe Fajardo Villela Antolin Barberena ◽  
Tainan da Silva Sousa ◽  
Nádia Roque

Abstract Área de Proteção Ambiental das Lagoas e Dunas do Abaeté (APA Abaeté) is an urban fragment of restinga in an environmentally protected area in the municipality of Salvador, Bahia, Brazil. The area is impacted by irregular disposal of solid waste, clandestine removal of sand from dunes, suppression and fragmentation of original vegetation cover and urban growth of neighborhoods. A recent floristic study revealed new records of orchid species for this conservation unit but highlighted strong threats to the orchid flora. In this context, a taxonomic study of Orchidaceae in APA Abaeté was undertaken with the aim of facilitating the recognition of species in the field and supporting the establishment of more effective local conservation actions for orchids, which should include the manual dispersion of seeds and the relocation of individuals. Orchidaceae is the sixth most represented family in APA Abaeté, with 15 genera and 19 species, including Encyclia dichroma and Koellensteinia florida, endemic species of the Atlantic Forest. The most relevant morphological characteristics for the identification of species in the area are: growth type; climbing habit; caulome thickness; shape and consistency of leaf blades; inflorescence type and position; and color and resupination of flowers.


2020 ◽  
Vol 9 (4) ◽  
pp. 157
Author(s):  
NFN SISWANTO ◽  
E. A. WIKARDI ◽  
E. KARMAWATI

<p>Dalam beberapa tahun terakhir tanaman jambu mete di NTB diserang oleh wercng pucuk. yang semula dikenal sebagai Lawana sp. atau L. Candida. Penamaan tersebut tidak tepat karena tidak didukung oleh penelitian taksonomi yang memadai. Penelitian ini bertujuan untuk mengidentifikasi serangga wereng pucuk tersebut berdasarkan ciri morfologi serta mcmpelajari beberapa aspek biologinya. Identiikasi dilakukan di Balai Penelitian Tanaman Rempah dan Obat (Balittro), Bogor dan Pusat Penelitian dan Pengembangan Biologi LIPI, Cibinong dari September - Nopember 2002, sedang pengamatan lapang di lakukan di daerah Lombok, NTB pada tahun 2001-2002. Dai identifikasi terhadap serangga contoh yang diambil dai beberapa lokasi di Lombok, NTB dapat disimpulkan bahwa wereng pucuk jambu mete tersebut adalah Sanurus indecora Jacobi. Beberapa cii utama serangga ini antara lain pada bentuk dan venasi sayap depan (legmen), jumlah spina (duri) lateral pada tibia kaki belakang, bentuk carina pada frons (muka bagian atas) dan bentuk aedeagus (genitalia jantan). Hasil pengamatan di lapangan menunjukkan bahwa daerah sebaran serangga tersebut terdapat di daerah Lombok dan Sumbawa, mempunyai banyak inang antara lain mangga, jeruk, krotalaria, jarak, rambutan, bougcnville dan nangka, siklus hidup 3 -4 minggu, seekor imago mampu meletakkan telur 80 butir atau lebih serta mempunyai beberapa musuh alami antara lain parasitoid telur, Aphanomerus sp. dan jamur entomopatogen, Synnematium sp. dan atau Hirsulella citriformis.</p><p>Kata kunci: Wereng pucuk, jambu mete, Sanurus indecora</p><p> </p><p><strong>ABSTRACT</strong></p><p><strong>Identiication of cashew shoot hopper, S. indecora and its biological aspects</strong></p><p>In the last few years, cashew plantations in West Nusa Tenggara has been attacking by shoot hopper, which formerly known as Lawana sp. or L. Candida. The naming was incorrect as there was not supported by accurate taxonomic study. The research was aimed at identifying the insect based on its morphological characteristics and studying its biological aspects in the ield. Identification was carried out in Balittro (Research Institute for Spice and Medicinal Crops), Bogor and Biological Research and Development Centre LIPI, Cibinong from September Nopember 2002, while ield observation was carried out in Lombok, NTB duing 2001-2002. Identification result showed that the shoot hopper is Sanurus indecora Jacobi. Some important characteristics of the insect arc the form and venation of tegmen, number of metatibial spina, form of carina on the frons and Ihe aedeagus. Results of the ield observation showed that the insect spreads in Lombok and Sumbawa. The alternative host plants are mango, citrus, Crolalaria, Ricmus commums, rambutan, Bougenville and Jackfruit. Its life cycle is 3 -4 weeks, female could lay 80 eggs or more. They have some natural enemies such as egg parasitoid, Aphanomerus sp. and entomopathogen fungi. Synnematium sp. or Hirsulella cilnformts.</p><p>Key words : Shool hopper, Anacardium occidentale. Sanurus indecora</p>


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