scholarly journals Partial characterization of alkaline proteases

2014 ◽  
Vol 11 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Bogusław Narzymski ◽  
Jadwiga Chmielnicka
Keyword(s):  
Culture Filtrate ◽  
Proteolytic Enzymes ◽  
Defined Medium ◽  
Partial Characterization ◽  
Alkaline Proteases ◽  
Chemically Defined Medium

When <i>Mucor Microsporus</i> Nam. was grown in a chemically defined medium alkaline proteases produced by this organisms appeares in the culture filtrate. Crude proteolytic enzymes preparations isolated from these filtrates show two optima for thedigestion of casein, namely at pH 8 and 11.

Fractionation and Partial Characterization of the Proteolytic Enzymes of Stem Bromelain*

Biochemistry ◽  
1963 ◽  
Vol 2 (3) ◽  
pp. 476-481 ◽  
Author(s):  
Mohamed El-Gharbawi ◽  
John R. Whitaker
Keyword(s):  
Proteolytic Enzymes ◽  
Partial Characterization ◽  
Stem Bromelain

scholarly journals Physiological Study of Lactobacillus delbrueckii subsp. bulgaricus Strains in a Novel Chemically Defined Medium

2000 ◽  
Vol 66 (12) ◽  
pp. 5306-5311 ◽  
Author(s):  
Christian Chervaux ◽  
S. Dusko Ehrlich ◽  
Emmanuelle Maguin
Keyword(s):  
Carbon Sources ◽  
Streptococcus Thermophilus ◽  
Defined Medium ◽  
Lactobacillus Delbrueckii ◽  
Protein Labeling ◽  
Chemically Defined Medium ◽  
Phosphotransferase System ◽  
Nutritional Conditions ◽  
Labeling Method

ABSTRACT We developed a chemically defined medium called milieu proche du lait (MPL), in which 22 Lactobacillus delbrueckii subsp.bulgaricus (L. bulgaricus) strains exhibited growth rates ranging from 0.55 to 1 h−1. MPL can also be used for cultivation of other lactobacilli and Streptococcus thermophilus. The growth characteristics of L. bulgaricus in MPL containing different carbon sources were determined, including an initial characterization of the phosphotransferase system transporters involved. For the 22 tested strains, growth on lactose was faster than on glucose, mannose, and fructose. Lactose concentrations below 0.4% were limiting for growth. We isolated 2-deoxyglucose-resistant mutants from strains CNRZ397 and ATCC 11842. CNRZ397-derived mutants were all deficient for glucose, fructose, and mannose utilization, indicating that these three sugars are probably transported via a unique mannose-specific-enzyme-II-like transporter. In contrast, mutants of ATCC 11842 exhibited diverse phenotypes, suggesting that multiple transporters may exist in that strain. We also developed a protein labeling method and verified that exopolysaccharide production and phage infection can occur in MPL. The MPL medium should thus be useful in conducting physiological studies ofL. bulgaricus and other lactic acid bacteria under well controlled nutritional conditions.

Partial characterization of alkaline proteases from viscera of vermiculated sailfin catfish Pterygoplichthys disjunctivus Weber, 1991

2011 ◽  
Vol 77 (4) ◽  
pp. 697-705 ◽  
Author(s):  
Ana Gloria Villalba-Villalba ◽  
Ramón Pacheco-Aguilar ◽  
Juan Carlos Ramirez-Suarez ◽  
Elisa Miriam Valenzuela-Soto ◽  
Francisco Javier Castillo-Yáñez ◽  
...  
Keyword(s):  
Partial Characterization ◽  
Alkaline Proteases

scholarly journals Purification and partial characterization of the gastric ulcer inhibitory substance from culture filtrate of Bacillus subtilis H.

1977 ◽  
Vol 25 (10) ◽  
pp. 2770-2774 ◽  
Author(s):  
TSUTOMU MIMURA ◽  
NORIO MUTO ◽  
HIROSHI TSUJIBO ◽  
NOBORU ONISHI ◽  
SHIGERU AONUMA
Keyword(s):  
Bacillus Subtilis ◽  
Gastric Ulcer ◽  
Culture Filtrate ◽  
Partial Characterization ◽  
Inhibitory Substance
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