Geosmithia species associated with fir-infesting beetles in Poland
<em>Geosmithia</em> species (Ascomycota: Hypocreales) are common ectosymbionts of scolytine bark and ambrosia beetles that feed on coniferous and deciduous trees in different forest ecosystems. <em>Geosmithia morbida</em> is the canker pathogen that causes extensive mortality of <em>Juglans nigra</em>. Because little is known regarding the <em>Geosmithia</em> species on European silver fir (<em>Abies alba</em>), we have investigated the diversity and abundance of these fungi associated with insects infesting European silver fir in Poland. Samples associated with eight beetle species were collected from three fir forests. Fungi were isolated from beetles and galleries. Isolates were identified based on morphological characteristics, DNA sequence comparison for three gene regions (ITS, ßT, TEF1-α), and phylogenetic analyses. <em>Geosmithia</em> was detected in 33% of the total 531 beetle samples obtained from <em>A. alba</em>. Two undescribed species of <em>Geosmithia</em> were distinguished, <em>Geosmithia</em> sp. 9 and <em>Geosmithia</em> sp. 16. Associations of <em>Pityokteines</em> spp. with <em>Geosmithia</em> fungi were recorded for the first time. <em>Pityokteines vorontzowi</em> and <em>Pityophthorus pityographus</em> appear to be regular vectors for <em>Geosmithia</em> sp. 9 and <em>Geosmithia</em> sp. 16, respectively. <em>Pityokteines curvidens</em> and <em>Cryphalus piceae</em> were associated with <em>Geosmitha</em> sp. 9 at lower frequencies.