scholarly journals Effectiveness of Daphne L. (Thymelaeaceae) in vitro propagation, rooting of microshoots and acclimatization of plants

2012 ◽  
Vol 65 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Ewa Hanus-Fajerska ◽  
Alina Wiszniewska ◽  
Przemysław Czaicki
Keyword(s):  
Plant Material ◽  
In Vitro Propagation ◽  
Shoot Formation ◽  
Mineral Salts ◽  
Shrub Species ◽  
Multiplication Coefficient ◽  
Shoot Propagation ◽  
Propagation Media

In this study, an attempt was made to investigate <i>in vitro</i> morphogenetic competence of three shrub species from the <i>Thymelaeaceae</i> family. The studied plant material originated from Russia, Greece and China, and the effectiveness of <i>in vitro</i> shoot formation and rhizogenesis of <i>Daphne caucasica</i>, <i>D. jasminea</i>, and <i>D. tangutica</i> was verified. The multiplication coefficient was compared for different propagation media. Medium composed of WPM mineral salts, MS microelements and a set of vitamins, supplemented with 1.0 mg dm<sup>-3</sup> 2iP, 0.1 mg dm<sup>-3</sup> NAA, and 0.65 g dm<sup>-3</sup> calcium gluconate, was appropriate for micropropagation of the tested genotypes. Shoot propagation in medium containing B<sub>5</sub> vitamins and microelements was not as effective as on WPM/MS medium. The rooting phase, especially in <i>D. tangutica</i>, needs further optimization in order to reduce the costs associated with acclimatization of microplantlets obtained to <i>in vivo</i> conditions. After stabilization, the plants were successfully cultivated under greenhouse conditions.

scholarly journals In vitro germination and bulblet and shoot propagation for wild edible Eremurus spectabilis M.Bieb.

2020 ◽  
Vol 48 (2) ◽  
pp. 814-825
Author(s):  
Burcu TUNCER
Keyword(s):  
In Vitro Propagation ◽  
Shoot Formation ◽  
Dichlorophenoxyacetic Acid ◽  
In Vitro Germination ◽  
Cut Flower ◽  
Nutrient Media ◽  
The Third ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Shoot Propagation

Eremurus spectabilis M.Bieb is consumed as a vegetable because of its nutritious characteristics. The plants are also used for medicinal purposes, in the cut flower industry as an ornamental geophytes, and in industry as a natural adhesive. The aim of the present study was to improve the in vitro propagation protocol (in vitro germination and bulblet and shoot formation) of E. spectabilis. For this purpose, E. spectabilis seeds were in vitro germinated in four different nutrient media: Murashige and Skoog (MS), Gamborg (B5), White (WH), and Shenk and Hildebrandt (SH). To stimulate bulblet and/or shoot regeneration, hypocotyls of 35-40-day-old in vitro-germinated plantlets were cut into 0.5-1.0 cm pieces, and the resultant explants were cultured in MS media containing 2,4-dichlorophenoxyacetic acid (2,4-D) (0.5, 1.0, 2.0, and 4.0 mg L-1) + Kinetin (0.5 mg L-1), Thidiazuron (TDZ) (0.5, 1.0, 2.0, and 4.0 mg L-1) + 1-Naphthylacetic acid (NAA) (0, 0.1, 0.5, and 1.0 mg L-1), and 6-Benzylaminopurine (BAP) (0.5, 1.0, 2.0, and 4.0 mg L-1) + 2,4-dichlorophenoxyacetic acid (2,4-D) (0, 0.1, 0.5, and 1.0 mg L-1). The best outcomes for germination ratio (57.5%) were obtained from the B5 medium. In the third set of in vitro propagation experiments, 100% bulblet formation was achieved in TDZ (0.5 mg L-1) and NAA (0.5 and 0.1 mg L-1) combinations of MS media, and this was followed by 0.5 mg L-1 BAP-containing medium (81.3%). Shoot formation ratios with the same media combinations varied from 60-70%, and the number of shoots per explant varied from 1.4-2.4 shoots. Further in vitro propagation research is planned with larger bulb sizes to develop a protocol for rooting bulblets and/or shoots.

scholarly journals Influence of Light Conditions and Medium Composition on Morphophysiological Characteristics of Stevia rebaudiana Bertoni In Vitro and In Vivo

Horticulturae ◽  
2021 ◽  
Vol 7 (7) ◽  
pp. 195
Author(s):  
Alla A. Shulgina ◽  
Elena A. Kalashnikova ◽  
Ivan G. Tarakanov ◽  
Rima N. Kirakosyan ◽  
Mikhail Yu. Cherednichenko ◽  
...  
Keyword(s):  
Culture Media ◽  
Stevia Rebaudiana ◽  
Adventitious Shoot ◽  
Optimal Growth ◽  
Medium Composition ◽  
Light Spectra ◽  
Stevia Rebaudiana Bertoni ◽  
Multiplication Coefficient

We investigated the influence of different conditions (light composition and plant growth regulators (PGRs) in culture media) on the morphophysiological parameters of Stevia rebaudiana Bertoni in vitro and in vivo. Both PGRs and the light spectra applied were found to significantly affect plant morphogenesis. During the micropropagation stage of S. rebaudiana, optimal growth, with a multiplication coefficient of 15, was obtained in an MS culture medium containing 2,4-epibrassinolide (Epin) and indole-3-acetic acid (IAA) at concentrations of 0.1 and 0.5 mg L−1, respectively. During the rooting stage, we found that the addition of 0.5 mg L−1 hydroxycinnamic acid (Zircon) to the MS medium led to an optimal root formation frequency of 85% and resulted in the formation of strong plants with well-developed leaf blades. Cultivation on media containing 0.1 mg L−1 Epin and 0.5 mg L−1 IAA and receiving coherent light irradiation on a weekly basis resulted in a 100% increase in the multiplication coefficient, better adventitious shoot growth, and a 33% increase in the number of leaves. S. rebaudiana microshoots, cultured on MS media containing 1.0 mg L−1 6-benzylaminopurine (BAP) and 0.5 mg L−1 IAA with red monochrome light treatments, increased the multiplication coefficient by 30% compared with controls (white light, media without PGRs).

scholarly journals Comparative Characteristics of Determination of Antioxidant Activity of Sea Buckthorn Fruits by Various Methods

2019 ◽  
Vol 8 (4) ◽  
pp. 48-52
Author(s):  
O. V. Trineeva
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
Plant Material ◽  
Natural Antioxidants ◽  
Sea Buckthorn ◽  
In Vitro Tests ◽  
Medicinal Plant Material

Introduction. Recently, much attention has been paid to the primary assessment of the pharmacological effect of various drugs using in vivo and in vitro tests. It is known that such a medicinal plant as sea buckthorn, in its phytochemical composition is rich in natural antioxidants: carotenoids, tocopherols, flavonoids, ascorbic acid, etc. In some publications there is information about the antioxidant activity of sea buckthorn and fatty oil based on them. However, information on the comparative characteristics of the use of various methods for determining the antioxidant activity of this type of medicinal plant material and the results obtained are not found in the scientific literature.Aim. The aim of this work was a comparative determination of the antioxidant activity of medicinal plant material of buckthorn fruits of various species of buckthorn.Materials and methods. The total antioxidant activity of water and water-alcohol extracts from the fruits of sea buckthorn fruits was determined using various techniques recommended in the literature. The antioxidant activity of the extracts was determined by permanganometric titration, in vitro inhibition of adrenaline autooxidation, and also in a biological model, Parametium caudatum cell culture.Results and discussion. The effect of the extractant polarity on the value of antioxidant activity was studied. It was found that the highest content of antioxidants in the extraction is observed when using 96 % ethanol as an extractant.Conclusion. Using three methods, the prospects of using sea buckthorn fruits and preparations based on them as a source of antioxidants are shown. 

scholarly journals Effect of Jasmonic Acid and Salicylic Acid on Growth and Biochemical Composition of In-Vitro-Propagated Lavandula angustifolia Mill

Agronomy ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1722
Author(s):  
Ileana Miclea ◽  
Andreea Suhani ◽  
Marius Zahan ◽  
Andrea Bunea
Keyword(s):  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Plant Material ◽  
Culture Media ◽  
Carotenoid Content ◽  
Lavandula Angustifolia ◽  
Beneficial Impact ◽  
Free Media

This study assessed the effect of jasmonic acid (JA) and salicylic acid (SA) on the in vitro development and production of Lavandula angustifolia Mill. plant material, and the accumulation of polyphenols, chlorophylls, and carotenoids in explants. Results were compared with explants grown in control media and with in-vivo-grown mature and young L. angustifolia plants. After 21 days of incubation, all explants propagated on low-SA-concentration or elicitor-free media produced a greater number of shoots than explants cultivated on media with higher elicitor concentrations. Shoots grew taller when activated charcoal (AC) was added to the elicitor-supplemented media, while AC negatively affected or had no effect on the phytochemical composition of plants. Explants grown in the presence of elicitors had higher polyphenolic and chlorophyll content than the controls, demonstrating the beneficial impact of elicitors on the secretion of secondary metabolites. Lutein and β-carotene were the dominating carotenoids in all samples. Culture media supplemented with 0.5 mg/L JA and 1.5 mg/L SA + AC proved the most suitable to produce plant material with high polyphenol and carotenoid content, comparable with in-vivo-grown plants.

scholarly journals GIS-Facilitated Effective Propagation Protocols of the Endangered Local Endemic of Crete Carlina diae (Rech. f.) Meusel and A. Kástner (Asteraceae): Serving Ex Situ Conservation Needs and Its Future Sustainable Utilization as an Ornamental

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1465
Author(s):  
Katerina Grigoriadou ◽  
Virginia Sarropoulou ◽  
Nikos Krigas ◽  
Eleni Maloupa ◽  
Georgios Tsoktouridis
Keyword(s):  
Added Value ◽  
Ex Situ ◽  
Sustainable Utilization ◽  
Ms Medium ◽  
Sustainable Exploitation ◽  
Local Endemic ◽  
Species Specific ◽  
Shoot Propagation

Conservation and sustainable exploitation of threatened endemic plants with medicinal and/or horticultural/ornamental value can be achieved through the development of effective propagation protocols. After unveiling the bioclimatic preferences of Carlina diae (Asteraceae) with geographic information systems (GIS), four propagation trials were conducted using seeds of this endangered local Cretan endemic for in vivo and in vitro germination, as well as seasonal vegetative propagation trials (softwood cuttings) and micropropagation (nodal explants). Seed germination was accomplished at a level of 77–90% in vivo (30 days) and 96% in vitro (10 days) using an MS medium with 2.9 μM gibberellic acid (GA3). The optimum treatments for cuttings’ rooting were 1000 and 2000 ppm indole-3-butyric acid (IBA) (11–16 roots, 2–3 cm long, 100% rooting) within 40 days in mist. In vitro shoot propagation exhibited a 2.8 proliferation rate after six successive subcultures on an MS medium with 2.9 μM GA3. Both ex vitro rooting and acclimatization were successful in 40 days, with 96% microshoot rooting and an equal survival rate. The GIS-facilitated effective species-specific propagation protocols developed in this study can consolidate the perspective of successful re-introduction of ex situ-raised material of C. diae into wild habitats and may serve its sustainable exploitation for high-added value ornamental products.

scholarly journals 882 PB 276 IN VITRO PROPAGATION AND CHIMERAL TRAITS OF Cryptanthus `Marian Oppenheimer' (WIDE LEAF CLONE)

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 560c-560
Author(s):  
Yong Cheong Koh ◽  
Fred T. Davies
Keyword(s):  
In Vitro Propagation ◽  
Callus Formation ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Induction Medium ◽  
Ms Medium ◽  
Periclinal Chimera ◽  
Adventitious Shoot Formation ◽  
L1 And L2

The leaves of vegetative stolons of greenhouse grown Cryptanthus `Marian Oppenheimer' (wide leaf clone) were cultured in modified MS media to induce adventitious shoot formation via callus formation. The best callus induction medium was basal MS medium with 10 μM NAA, IBA and BA. Pure green (843), maroon (3), striped (2) and albino plantlets were obtained. Most of the albino plantlets were stunted, tightly clumped together and impossible to score. The medium which produced the highest average number of non-albino plantlets was basal MS medium with 0.3 μM NAA, IBA and BA All non-albino plantlets were rooted in MS medium with 5.4 μM NAA and transplanted ex vitro with a survival rate of 96.7%. The maroon plantlets became green two weeks after transplanting. Histological studies revealed that C. `Marian Oppenheimer' (wide leaf clone) has two tunicas (L1 and L2) and a corpus (L3). Callus on the leaf explant arose mainly from the L2 and L3. Apparently C. `Marian Oppenheimer' (wide leaf clone) is a GWG periclinal chimera.

scholarly journals ECONOMICAL STUDY OF IN VIVO AND IN VITRO PROPAGATION OF RUSSELIA EQUISETIFORMIS

2018 ◽  
Vol 5 (1) ◽  
pp. 67-87
Author(s):  
M. Meshaal ◽  
K. Emam ◽  
F. Ghareb ◽  
E. Khella
Keyword(s):  
In Vitro Propagation

scholarly journals IN VITRO BREEDING AND NURSING TAQUA BANANA IN TRA VINH PROVINCE

2019 ◽  
Vol 1 (26) ◽  
pp. 102-108
Author(s):  
Tam Thi Thanh Dinh ◽  
Nam Quoc Phan ◽  
Trai Ngoc Nguyen ◽  
An Van Vo ◽  
Phuong Minh Nguyen
Keyword(s):  
In Vitro Propagation ◽  
Ms Medium ◽  
Dark Condition ◽  
Bud Regeneration ◽  
Adenine Sulfate ◽  
Good For ◽  
Optimal Medium ◽  
Propagation Media

The goal of this study is to determine in vitro propagation media of Taqua banana. The results showed that: the optimal medium for banana bud regeneration was MS medium (Murashine & Skoog 1962) supplemented 0.1 mg/l NAA, 100 mg/l adenine sulfate, 30g/l sucrose, 8 g/l Agar, 5 mg/l BAP, 10% volume coconut juice and kept in completely dark condition. The medium, which is similar to bud generation media except for supplementing BAP 7 mg/l, was also good for bud replication with  6,33 shoots per sample after 4 weeks. (2)The medium, which is similar to bud generation media except for non-added BAP and an increase of NAA from 0.1 to 1 mg/l, was the best for the banana rooting.

scholarly journals The In Vitro and In Vivo Effects of Hypoxis hemerocallidea on Indinavir Pharmacokinetics: Modulation of Efflux

Planta Medica ◽  
2018 ◽  
Vol 84 (12/13) ◽  
pp. 895-901
Author(s):  
Kaylee Havenga ◽  
Efrem Abay ◽  
Lubbe Wiesner ◽  
Alvaro Viljoen ◽  
Dewald Steyn ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Plant Material ◽  
Commercial Product ◽  
In Vivo Model ◽  
Cell Monolayers ◽  
Reference Plant ◽  
The Impact ◽  
Hypoxis Hemerocallidea

Abstract Hypoxis hemerocallidea (African potato) is a popular medicinal plant that has been used traditionally for the treatment of various disorders. Some HIV/AIDS patients use this traditional medicine together with their antiretroviral therapy. This study aimed to determine the impact of selected H. hemerocallidea materials (i.e., a commercial product, an aqueous extract, and biomass reference plant material) on the bidirectional permeability of indinavir across Caco-2 cell monolayers as well as the bioavailability of indinavir during an acute, single administration study in Sprague-Dawley rats. All of the selected H. hemerocallidea test materials demonstrated inhibition effects on indinavir efflux across Caco-2 cell monolayers, albeit to different extents. An increase in the bioavailability of indinavir was obtained in vivo when administered concomitantly with the H. hemerocallidea materials, albeit not statistically significantly. The change in bioavailability directly correlated with the in vitro permeability results. It can therefore be concluded that the change in permeability and bioavailability of indinavir was caused by efflux inhibition and this effect was dependent on the type of H. hemerocallidea material investigated, which was found to be in the following order: commercial product > aqueous extract > reference plant material. The clinical significance of the combined effect of efflux and metabolism inhibition by H. hemerocallidea should be determined in another in vivo model that expresses the cytochrome P450 3A4 enzyme.

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