scholarly journals Changes in structure of red pepper (Capsicum annuum L.) seedlings shoots under aluminum stress conditions

2012 ◽  
Vol 60 (1) ◽  
pp. 85-93 ◽  
Author(s):  
Agata Konarska
Keyword(s):  
Cell Wall ◽  
Capsicum Annuum ◽  
Red Pepper ◽  
Outer Cell ◽  
Aluminum Stress ◽  
Water Culture ◽  
Anatomical Features ◽  
Parenchyma Cells ◽  
Al Treatment ◽  
Outer Cell Wall

The seedlings of the red pepper (<i>Capsicum annuum</i> L.) cv. Trapez grown in water culture for a period of 14 days with Al (0, 10, 20 and 40 mg·dm<sup>-3</sup> AlCl<sub>3</sub>·6 H<sub>2</sub>O). Some morphological and anatomical features of red pepper shoots were analyzed. Reduction in height and diameter of stems as well as decrease in fresh mass of shoots were observed after Al-treatment. In the hypocotyl the thickness of cortex parenchyma layer and the size of their cells were reduced. The aluminum treatment resulted in the increased in thickness of the epidermis outer cell wall. Under Al stress in the cotrex and the central cylinder parenchyma cells were present numerous enlarge plastids which contained large grains of starch and dark little bodies which were possible aluminum deposits. They weren`t observed in control seedlings.

A freeze-etch study of plant cell walls for ectodesmata

1974 ◽  
Vol 52 (9) ◽  
pp. 2033-2036 ◽  
Author(s):  
N. C. Lyon ◽  
W. C. Mueller
Keyword(s):  
Cell Wall ◽  
Cell Walls ◽  
Leaf Tissue ◽  
Physicochemical Characteristics ◽  
Outer Cell ◽  
Plantago Major ◽  
Plant Cell Walls ◽  
Phaseolus Vulgaris L ◽  
Epidermal Cell Wall ◽  
Outer Cell Wall

Leaf tissue of Phaseolus vulgaris L. and Plantago major L. was prepared by the freeze-etch technique and examined in the electron microscope for the presence of ectodesmata. No structures analagous to ectodesmata observed with light microscopy could be found in freeze-etched preparations of chemically unfixed material or in material fixed only in glutaraldehyde. Objects appearing as broad, shallow, granular areas in the epidermal cell wall beneath the cuticle were observed in leaf replicas after fixation in complete sublimate fixative, the acid components of the sublimate fixative, or mercuric chloride alone. Because of their distribution and location, these objects can be considered analagous to ectodesmata observed by light microscopists. Because these areas occur only in chemically fixed walls and are localized within the walls in discrete areas, their presence supports the contention that ectodesmata are sites in the outer cell wall with defined physicochemical characteristics.

scholarly journals Spreading of a mycobacterial cell-surface lipid into host epithelial membranes promotes infectivity

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
CJ Cambier ◽  
Steven M Banik ◽  
Joseph A Buonomo ◽  
Carolyn R Bertozzi
Keyword(s):  
Cell Wall ◽  
Cell Surface ◽  
Immune Activation ◽  
Cholesterol Synthesis ◽  
Membrane Lipids ◽  
Preventive Therapy ◽  
Outer Cell ◽  
Surface Lipid ◽  
Epithelial Membranes ◽  
Outer Cell Wall

Several virulence lipids populate the outer cell wall of pathogenic mycobacteria. Phthiocerol dimycocerosate (PDIM), one of the most abundant outer membrane lipids, plays important roles in both defending against host antimicrobial programs and in evading these programs altogether. Immediately following infection, mycobacteria rely on PDIM to evade Myd88-dependent recruitment of microbicidal monocytes which can clear infection. To circumvent the limitations in using genetics to understand virulence lipids, we developed a chemical approach to track PDIM during Mycobacterium marinum infection of zebrafish. We found that PDIM's methyl-branched lipid tails enabled it to spread into host epithelial membranes to prevent immune activation. Additionally, PDIM’s affinity for cholesterol promoted this phenotype; treatment of zebrafish with statins, cholesterol synthesis inhibitors, decreased spreading and provided protection from infection. This work establishes that interactions between host and pathogen lipids influence mycobacterial infectivity and suggests the use of statins as tuberculosis preventive therapy by inhibiting PDIM spread.

Silver nanoparticles (AgNPs) internalization and passage through the Lactuca sativa (Asteraceae) outer cell wall

2021 ◽  
Author(s):  
Sergimar Kennedy de Paiva Pinheiro ◽  
Thaiz Batista Azevedo Rangel Miguel ◽  
Marlos de Medeiros Chaves ◽  
Francisco Claudio de Freitas Barros ◽  
Camila Pessoa Farias ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cell Wall ◽  
Lactuca Sativa ◽  
Outer Cell ◽  
Outer Cell Wall

Production of Monoclonal Antibodies Against the Outer Cell Wall ofClostridium tyrobutyricum

Hybridoma ◽  
1994 ◽  
Vol 13 (1) ◽  
pp. 45-51 ◽  
Author(s):  
FRANCOISE TALBOT ◽  
GEORGES ROBREAU ◽  
FRANCOISE GUEGUEN ◽  
ROGER MALCOSTE
Keyword(s):  
Cell Wall ◽  
Monoclonal Antibodies ◽  
Outer Cell ◽  
Outer Cell Wall

scholarly journals Conidial Hydrophobins of Aspergillus fumigatus

2003 ◽  
Vol 69 (3) ◽  
pp. 1581-1588 ◽  
Author(s):  
Sophie Paris ◽  
Jean-Paul Debeaupuis ◽  
Reto Crameri ◽  
Marilyn Carey ◽  
Franck Charlès ◽  
...  
Keyword(s):  
Cell Wall ◽  
Aspergillus Fumigatus ◽  
Amorphous Layer ◽  
Wall Layer ◽  
Host Cells ◽  
Outer Cell ◽  
Host Immune System ◽  
Wild Type ◽  
Cell Wall Layer ◽  
Outer Cell Wall

ABSTRACT The surface of Aspergillus fumigatus conidia, the first structure recognized by the host immune system, is covered by rodlets. We report that this outer cell wall layer contains two hydrophobins, RodAp and RodBp, which are found as highly insoluble complexes. The RODA gene was previously characterized, and ΔrodA conidia do not display a rodlet layer (N. Thau, M. Monod, B. Crestani, C. Rolland, G. Tronchin, J. P. Latgé, and S. Paris, Infect. Immun. 62:4380-4388, 1994). The RODB gene was cloned and disrupted. RodBp was highly homologous to RodAp and different from DewAp of A. nidulans. ΔrodB conidia had a rodlet layer similar to that of the wild-type conidia. Therefore, unlike RodAp, RodBp is not required for rodlet formation. The surface of ΔrodA conidia is granular; in contrast, an amorphous layer is present at the surface of the conidia of the ΔrodA ΔrodB double mutant. These data show that RodBp plays a role in the structure of the conidial cell wall. Moreover, rodletless mutants are more sensitive to killing by alveolar macrophages, suggesting that RodAp or the rodlet structure is involved in the resistance to host cells.

The effects of chlorpromazine on the outer cell wall of Salmonella typhimurium in ensuring resistance to the drug

2000 ◽  
Vol 14 (3) ◽  
pp. 225-229 ◽  
Author(s):  
Leonard Amaral ◽  
Jette E Kristiansen ◽  
Villy Frølund Thomsen ◽  
Bogooljub Markovich
Keyword(s):  
Cell Wall ◽  
Salmonella Typhimurium ◽  
Outer Cell ◽  
Outer Cell Wall

scholarly journals Anatomical features of leaves of sweet pepper (Capsicum annuum L.) fed with calcium using foliar nutrition

2012 ◽  
Vol 62 (2) ◽  
pp. 155-164 ◽  
Author(s):  
Elżbieta Weryszko-Chmielewska ◽  
Zenia Michałojć
Keyword(s):  
Capsicum Annuum ◽  
Sweet Pepper ◽  
Epidermal Cells ◽  
Outer Cell ◽  
Foliar Nutrition ◽  
Abaxial Epidermis ◽  
Major Barrier ◽  
Anatomical Features ◽  
Nutrient Supplements ◽  
Pepper Leaves

The effect of three foliar-applied Ca-containing preparations on the anatomical features of leaves of sweet pepper (<i>Capsicum annuum</i> L.) was studied. The following preparations were used: Ca(NO<sub>3</sub>)<sub>2</sub>, Librel Ca and Wapnowit, applied at the respective concentrations of 0.5%, 1%, 1%, which corresponded to a content of 2000 mg Ca × dm<sup>-3</sup>. Light and scanning electron microscopy were used in the study. It was demonstrated that in amphistomatic bifacial pepper leaves numerous specialised cells occurred which accumulated calcium oxalate crystals in the form of crystalline sand. Anisocytic stomata were found with a much greater density in the abaxial epidermis. They were characterized by very well-developed outer cuticular ledges. It was found that in the leaves of the plants sprayed with the nutrient supplements with increased Ca content there was a much smaller number of epidermal cells per 1 mm<sup>2</sup> than in the control plants. These cells were distinguished by an increased size. In the case of the application of the nutrient supplements Librel Ca and Wapnowit, the number of stomata also decreased. However, the application of the calcium supplements resulted in an increase in the value of the stomatal index compared to the control, which is attributable to a significant reduction in the number of epidermal cells not belonging to the stomata. The plants additionally supplied with Ca were marked by a larger number of colenchyma layers and an increased volume of leaf parenchyma cells. In the case of pepper leaves, the thin cuticle and the outer cell wall are not a major barrier to the Ca-containing preparations applied for spray treatment. Nevertheless, the decrease in the number of stomata may restrict the possibility of Ca uptake by this way, which compensates the increase in surface area of particular epidermal cells that will be the main way of Ca penetration into the internal leaf tissues.

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