scholarly journals Distribution and structure of internal secretory reservoirs on the vegetative organs of Inula helenium L. (Asteraceae)

2012 ◽  
Vol 60 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Aneta Sulborska
Keyword(s):  
Cross Sections ◽  
Light And Electron Microscopy ◽  
Secretory Cells ◽  
Vascular Bundles ◽  
Vegetative Organs ◽  
Full Flowering ◽  
Secretory Cavities ◽  
Inula Helenium ◽  
Glandular Cells ◽  
Large Central Vacuole

The aim of the study was to investigate the structure and topography of endogenous secretory tissues of <i>Inula helenium</i> L. By using light and electron microscopy, morphological and anatomical observations of stems, leaves and rhizomes were made. It was shown that in the stems secretory cavities were situated in the vicinity of phloem and xylem bundles. The number of the reservoirs reached its maximum value (34) at shoot flowerig termination, whereas the cavities with the largest diameter were observed at full flowering stage (44.6 µm). In the leaf petioles and midribs, the reservoirs also accompanied the vascular bundles, and their number and size increased along with the growth of the assimilation organs. Observations of the cross sections of the rhizomes revealed the presence of several rings of secretory reservoirs. The measurements of the cavities showed that as a rule the reservoirs with a larger dimension were located in the phelloderm, whereas the smallest ones in the xylem area. The secretory cavities located in the stems and leaves developed by schizogenesis, whereas the rhizome reservoirs were probably formed schizolisygenously. The cells lining the reservoirs formed a one - four-layered epithelium. Observed in TEM, the secretory cells of the mature cavities located in the rhizomes were characterised by the presence of a large central vacuole, whereas the protoplast was largely degraded. Fibrous elements of osmophilic secretion and numerous different coloured vesicles could be distinguished in it. The cell walls formed, from the side of the reservoir lumen, ingrowths into the interior of the epithelial cells. Between the cell wall and the plasmalemma of the glandular cells, a brighter periplasmatic zone with secretory vesicles was observed.

scholarly journals Anatomy and Micromorphology of Inula helenium subsp. orgyalis and I. ensifolia (Asteraceae) from Turkey

2017 ◽  
Vol 9 (1) ◽  
pp. 104-109
Author(s):  
Tulay AYTAS AKCIN ◽  
Adnan AKCIN
Keyword(s):  
Glandular Trichomes ◽  
Vascular Bundles ◽  
Secretory Cavities ◽  
Inula Helenium

Inula helenium L. subsp. orgyalis (Boiss.) Grierson and Inula ensifolia L. were investigated anatomically and micromorphologically. The secretory cavities in the leaves and stem of both investigated taxa were located in the neighbourhood of the vascular bundles and in the rhizomes in the secondary cortex. The leaf mesophylls of investigated Inula taxa were homogeneous. Stomata were anomocytic in two species. The distribution and density of the eglandular and glandular trichomes provide information of taxonomical significance. Moreover, the cypselas of I. helenium L. subsp. orgyalis were homomorphic, whereas in I. ensifolia cypselas were heteromorphic. Additionally, the number of ribs, the shape of carpopodium and stylopodium were diagnostic taxonomic characters between the two taxa.

scholarly journals Morphology, anatomy and ultrastructure of yarrow (Achillea millefolium L.) floral nectaries

2012 ◽  
Vol 59 (1) ◽  
pp. 17-28 ◽  
Author(s):  
Aneta Sulborska ◽  
Elżbieta Weryszko-Chmielewska
Keyword(s):  
Cell Ultrastructure ◽  
Achillea Millefolium ◽  
Secretory Cells ◽  
Vascular Bundles ◽  
Cell Nuclei ◽  
Golgi Bodies ◽  
Glandular Cells ◽  
A Cell ◽  
Electron Microscopes ◽  
Big Cell

The studies focused on the morphological and anatomical features as well as those related to the ultrastructure of nectary cells <i>Achillea millefolium</i> Asteraceae family. The nectary presence was confirmed only in the disk flowers at the pistil style base. The micromorphology of nectaries was investigated in SEM, and structure was observed in a light and transmission electron microscopes. A number of layers composing a gland, the size and shape of epidermal and glandular cells were determined. The secretory cell ultrastructure was analyzed. The discoidal nectary gland observed from above had a pentagonal shape, 181.5 µm height and 299.4 µm diameter. It was built of the monolayer epidermis and 6 layers of the secretory cells on average. The glandular cells appeared to be bigger (27 µm) than the epidermal cells (22 µm), a cell shape in both tissues differed as well. The nectar secretion occured through the modified stomata. The stomata cells were at distinguishable greater size and raised above the surface of epidermis. The nectaries were supplied by the vascular bundles running from the pistil style up to the nectary base, not getting into the gland. In the cells of the nectary epidermis observed in TEM the big cell nuclei, numerous plastids, mitochondria and vacuoles with fibrous secretion deposits and vesicular structures were found. In the cells of the nectary secretory tissue there were dense cytoplasm, many plastids, mitochondria, Golgi bodies and the extensive network of the endoplasmic reticulum.

scholarly journals Phenolic Compounds Produced by Secretory Structures in Plants: A Brief Review

2008 ◽  
Vol 3 (8) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
M. Castro Marilia De ◽  
Diego Demarco
Keyword(s):  
Phenolic Compounds ◽  
Pollen Tube ◽  
Pollen Germination ◽  
Chemical Defence ◽  
Complex Mixtures ◽  
Secretory Cells ◽  
Vascular Bundles ◽  
Secretory Structures ◽  
Vegetative Organs ◽  
Pollen Tube Elongation

The purpose of this brief review has been to provide more recent data regarding the production of phenolic compounds by secretory structures. Although morphology and histochemistry of glands are well documented, meagre information concerning phenolics is available in the surveyed literature. Two major groups of glands are found regarding phenolic compounds synthesis: 1. secretory cells producing mainly phenolics, 2. secretory cells producing phenolics coupled with other compounds. In the former group, phenolic compounds remain in mature organs, and prevail in the material produced by epidermis, hypodermis, idioblasts, and sheath encircling vascular bundles and ducts. The latter group is constituted of trichomes, cavities, ducts, laticifers, colleters, nuptial nectaries, osmophores and stigma system, which synthesize complex mixtures of terpenes, phenolic compounds, polysaccharides and other compounds. In vegetative organs, the secretion of these glands might provide chemical defence against damage by UV radiation, against pathogen activities, and play a role in the herbivory deterrence. Additional functions ascribed to phenolics produced by floral glands are associated with pollination, pollen germination and pollen-tube elongation.

The Response of Testis Cells to Low Dose X-Irradiation

1981 ◽  
Vol 39 ◽  
pp. 542-543
Author(s):  
D. E. Philpott ◽  
W. Sapp ◽  
C. Williams ◽  
Joann Stevenson ◽  
S. Black
Keyword(s):  
Electron Microscopy ◽  
Stem Cell ◽  
Light Microscopy ◽  
Dose Level ◽  
Cross Sections ◽  
Low Dose ◽  
Light And Electron Microscopy ◽  
Cellular Responses ◽  
Post Irradiation ◽  
X Irradiation

The response of spermatogonial cells to X-irradiation is well documented. It has been shown that there is a radiation resistent stem cell (As) which, after irradiation, replenishes the seminiferous epithelium. Most investigations in this area have dealt with radiation dosages of 100R or more. This study was undertaken to observe cellular responses at doses less than 100R of X-irradiation utilizing a system in which the tissue can be used for light and electron microscopy.Brown B6D2F1 mice aged 16 weeks were exposed to X-irradiation (225KeV; 15mA; filter 0.35 Cu; 50-60 R/min). Four mice were irradiated at each dose level between 1 and 100 rads. Testes were removed 3 days post-irradiation, fixed, and embedded. Sections were cut at 2 microns for light microscopy. After staining, surviving spermatogonia were identified and counted in tubule cross sections. The surviving fraction of spermatogonia compared to control, S/S0, was plotted against dose to give the curve shown in Fig. 1.

scholarly journals Morphological and phytochemical analysis of leaves and stems of Tetrapterys multiglandulosa CAV.

2020 ◽  
pp. 959-964
Author(s):  
Gilberto Gonçalves Facco ◽  
Eloty Justina Schleder ◽  
Natalia Yoshioka de Vidis ◽  
Maristela Halverson ◽  
Rosemary Matias ◽  
...  
Keyword(s):  
Cross Sections ◽  
Clinical Signs ◽  
Single Layer ◽  
Anatomical Study ◽  
Phytochemical Analysis ◽  
Native Plant ◽  
Circular Section ◽  
Vegetative Organs ◽  
Ethanol Extracts ◽  
Columnar Cells

This study aimed to carry out morphoanatomical, histochemical and phytochemical analysis of leaves and stems of Tetrapterys multiglandulosa Cav. (Malpighiaceae), a native plant responsible for abortion and sudden death in beef cattle. Plant specimens underwent anatomical study, in which leaves and stems were dried and ground with aqueous and ethanol extracts subjected to histochemical and phytochemical analysis. Anatomical observation of vegetative organs detected the presence of simple columnar cells in the adaxial epidermis, palisade mesophyll, parenchyma with a layer of cells and paracitic type stomata. Stem cross-sections presented circular section, single layer (uniseriate) epidermis with thick cuticle and Malpighi’s trichomes, the phelogen was in differentiation. The cortex showed angular collenchyma, parenchyma with exogenous channels and druse shaped calcium oxalate crystals. Phytochemical tests of aqueous and ethanol extracts of leaves and stems indicated the presence of phenolic compounds, tannins, flavonoids, saponins, alkaloids and cardiotonic heterosides. Potentially phytotoxic secondary metabolites evidenced in the leaves and stems, including saponins and cardiotonic heterosides were correlated with clinical signs observed in intoxicated animals.

Ultrastructure of scent glands in larvae of Apateticus bracteatus (Hemiptera: Pentatomidae) and chemical composition of the secretion

1980 ◽  
Vol 58 (11) ◽  
pp. 2105-2115 ◽  
Author(s):  
Jean Percy ◽  
J. A. MacDonald ◽  
J. Weatherston
Keyword(s):  
Chemical Composition ◽  
Interstitial Cells ◽  
Secretory Cells ◽  
Scent Glands ◽  
Volatile Constituents ◽  
Dorsal Wall ◽  
Anterior Dorsal ◽  
Glandular Cells ◽  
Gland Function ◽  
Dorsal Abdominal Glands

The three dorsal abdominal glands in larvae of Apateticus bracteatus (Pentatomidae) secrete a mixture of compounds. Major volatile constituents of the secretion are identified, herein, as tridecane and 2-octenal. There are also trace amounts of 2-hexenal and two other unidentified compounds.Each of the glands has paired orifices that are located between tergites 3/4, 4/5, and 5/6, but only the most anterior gland is paired. In anterior glands of midinstar larvae, glandular cells associated with ducts, and interstitial glandular cells are distributed along the ventral walls of the reservoirs. In posterior glands, columnar glandular cells are located in the anterior dorsal wall of the reservoirs; secretory cells associated with ducts, and nonglandular interstitial cells are distributed throughout the ventral and posterior walls of the reservoirs. The interstitial glandular cells of the anterior gland and the columnar glandular cells of the middle and posterior glands contain cytoplasmic organelles characteristic of lipid-producing cells. In all glands the secretory cells associated with ducts secrete lipids. Evidence indicating the importance of Golgi and ER in secretion synthesis is presented. The reservoirs and ducts have a thin cuticular lining.The bearing of the results on present ideas of gland function in Heteroptera is discussed.

Experimental Data on the Function of the Interstitium of the Gonads: Experiments with Cockerels

1947 ◽  
Vol s3-88 (2) ◽  
pp. 135-150
Author(s):  
J. W. SLUITER ◽  
G. J. VAN OORDT
Keyword(s):  
Experimental Data ◽  
Connective Tissue ◽  
Leydig Cells ◽  
Cell Types ◽  
Sex Hormone ◽  
Secretory Cells ◽  
Secretory Function ◽  
Glandular Cells ◽  
Male Sex ◽  
Secondary Function

1. The relative volumes of the testes and their components of 31 cockerels, 2-200 days old, were calculated and compared with the size of their increasing head appendages (Text-figs. 1a-d, 2); in addition, the effect of gestyl-administration on testes of cockerels of this age was investigated. 2. Several types of interstitial testis-cells could be distinguished morphologically and physiologically (Text-figs. 3-6 and Pl. 1); these cell-types were studied with different techniques and counted separately. 3. The main types of the interstitial cells are: (a) Lipoid cells, totally packed with lipoid globules. These cells, which are considered by many authors as fully developed Leydig cells, are not directly connected with the production of the male sex hormone; perhaps they have a secondary function in this respect, as cholesterolderivatives are stored in these cells (Pl. 1, Text-fig. 3a). (b) Secretory cells, characterized by the absence of lipoid vacuoles and the presence of numerous granular and filamentous mitochondria. These secretory cells, which produce the male sex hormone, can be divided into secretory cells A (Text-fig. 6a) without, and secretory cells B with, one large vacuole (Text-figs. 6b, 6c, 6d). 4. A considerable and partly intercellular storage of lipoids may take place at any age in the intertubular connective tissue (Text-figs. 3-4 and Pl. 1). 5. The number of the lipoid cells depends on the nutritive conditions of the animal and the development of its testes (Text-fig. 7). 6. In older cockerels most of the glandular cells lose their secretory function and pass over into lipoid storing cells. 7. Therefore we agree with Benoit, when he denies the occurrence of a ‘secretion de luxe’, but we cannot accept the presence of a ‘parenchyme de luxe’ in the testes of older cockerels.

scholarly journals Struktur Anatomi dan Uji Histokimia Terpenoid dan Fenol Dua Varietas Sirih Hijau (Piper betle L.)

BIOSCIENTIAE ◽  
2021 ◽  
Vol 17 (2) ◽  
pp. 1
Author(s):  
Gusti Puspa Dewi ◽  
Evi Mintowati Kuntorini ◽  
Eny Dwi Pujawati
Keyword(s):  
Copper Acetate ◽  
Anatomical Structure ◽  
Secretory Cells ◽  
Cell Diameter ◽  
Piper Betle ◽  
Vascular Bundles ◽  
Palisade Parenchyma ◽  
Betel Leaf ◽  
Epidermal Tissue ◽  
Pith Parenchyma

This study aims to determine the anatomical structure and histochemical test of terpenoid and phenol compounds in two varieties of green betel plants (Piper betle). Making leaves anatomical structure preparations using the fresh method, testing terpenoid compounds with 5% copper acetate, testing phenol with ferric trichloride 10% and some grains of sodium carbonate. The observations of the anatomical structure of green betel leaf varieties 1 and varieties 2 have similarities consisting of the upper epidermis, upper hypodermis, palisade parenchyma, parenchymal sponges, vascular bundles (xylem and phloem), sclerenchyma, cholenchyma, lower epidermis, lower hypodermis, secretory cells, trichoma, stoma and calcium oxalate crystals, and in varieties 2 look more trichomes. The anatomical structure of the variety 1 betel stem and varieties 2 are arranged from the outside in the direction of the epidermal tissue, colenchymal tissue, cortical bundles, sclerenchyma, cortex, medullary and peripheral vascular files, pith, the central part of the stem is a secretory gland. Phenol in betel vine varieties 1 and varieties 2 is positive in the secretion cell part which is spread in the parenchymal tissue of the mother's leaf bone and lamina, whereas in the stem is spread around the cortex and pith parenchyma. Positive secretion cells contain phenol not as much as secretory cells containing terpenoids. Based on quantitative observations the size of oil cell density and secretion cell diameter, the essential oils contained in the cell secretions in the leaves of variety 1 are more than varieties 2 while in the varieties 2, there are more varieties 1.

scholarly journals The anatomy as a tool for the identification of the bark of Pterocarpus angolensis and Terminalia sericea

2020 ◽  
Vol 7 (1) ◽  
pp. 925
Author(s):  
Teresa Quilhó ◽  
Fernanda Bessa ◽  
Ana Isabel Ribeiro-Barros ◽  
Natasha Ribeiro
Keyword(s):  
Plant Material ◽  
Light And Electron Microscopy ◽  
Secretory Cells ◽  
Correct Identification ◽  
Large Crystal ◽  
African Species ◽  
Fibre Bundles ◽  
Anatomical Features ◽  
Pterocarpus Angolensis ◽  
Crystal Cells

Pterocarpus angolensis and Terminalia sericea are two African species with medicinal potential. Despite the importance of their bark as a powerful astringent to treat various diseases it is poor described. In order to provide referential information for correct identification and standardization of the plant material, bark samples from each two species were collected and analyzed under light and electron microscopy. Some important anatomical features to identification were: the sclerenchyma tissue mostly in form of fibre-sclereids and the large secretory cells arranged in conspicuous rows or tangential bands in the conducting phloem in P. angolensis; the crystalliferous cells arranged in very regular tangential rows (druses) and the occurrence of large crystal cells near or including the tangential fibre bundles in T. sericea bark.The results obtained show that the anatomy of the bark can be used as an important subsidy in identification and standardization of the studied species contributing the scientific knowledge for more effective forms of scrutiny in preventing commercial adulteration of species.

scholarly journals Micromorphology and ultrastructure of trichomes of Libyan Salvia fruticosa Mill.

2013 ◽  
Vol 65 (1) ◽  
pp. 239-246 ◽  
Author(s):  
Sheef Al ◽  
Sonja Duletic-Lausevic ◽  
Dusica Janosevic ◽  
Snezana Budimir ◽  
Marija Marin ◽  
...  
Keyword(s):  
Glandular Trichomes ◽  
Light And Electron Microscopy ◽  
Stalk Cell ◽  
Secretory Cells ◽  
Salvia Fruticosa ◽  
Short Stalk ◽  
Capitate Trichomes ◽  
Leaf Surfaces ◽  
Large Round ◽  
Peltate Trichomes

Micromorphological and ultrastructural analyses of the leaf trichomes of Salvia fruticosa Mill. were performed by light and electron microscopy. The leaves bear numerous non-glandular unbranched trichomes, and peltate, capitate and digitiform glandular trichomes. Very elongated flagelliform non-glandular trichomes densely covered the leaf surfaces, with especially abundance on the leaf margins. Peltate trichomes consist of a basal epidermal cell, a very short stalk cell and a large round head of eight secretory cells arranged in a circle. Capitate trichomes can be divided into two main types, short-stalked and long-stalked, and further into five subtypes according to the number of stalk cells, morphology and number of glandular head cells. Digitiform trichomes consist of one basal cell, one or two stalk cells and one apical secretory cell, which are of similar diameter and approximately equal length.

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