scholarly journals Chitosan biopolymer improves the fruit quality of litchi (Litchi chinensis Sonn.)

2019 ◽  
Vol 72 (2) ◽  
Author(s):  
Md. Shabab Mehebub ◽  
Nur Uddin Mahmud ◽  
Mosaddiqur Rahman ◽  
Musrat Zahan Surovy ◽  
Dipali Rani Gupta ◽  
...  

Chitosan (CHT) is a natural compound that has been used to control postharvest pathogenic diseases due to its capability of eliciting natural defense responses in plants. The aim of this study was to investigate the effect of foliar CHT application on yield and quality of the litchi fruit. Chitosan was applied by spraying on to fruit and foliage just after fruit set four times at 7-day intervals with four varying doses viz. 100, 250, 500, and 1,000 µg L<sup>−1</sup> and a control (0 µg L<sup>−1</sup>). Although the application of CHT had no significant effect on the size of the fruits (length and width), the total contents of phenolics, flavonoids, and ascorbic acid and the antioxidant activity of litchi fruit arils were significantly increased in CHT-treated fruit compared with the untreated control. The highest phenolic, flavonoid, and ascorbic acid contents were 334 µg gallic acid g<sup>−1</sup>, 881 μg quercetin g<sup>−1</sup>, and 178 µg g<sup>−1</sup>, respectively, in fruits treated with 500 µg L<sup>−1</sup> CHT. However, the highest antioxidant activity (622 μg butylated hydroxytoluene g<sup>−1</sup>) was recorded in 250 µg L<sup>−1</sup> CHT-treated fruits. Our findings revealed that the application of low doses of CHT in a litchi orchard might improve fruit quality by increasing the content of antioxidants and antioxidant activities.

2019 ◽  
Vol 15 ◽  
Author(s):  
Aleksandra Kładna ◽  
Paweł Berczyński ◽  
Oya Bozdağ Dündar ◽  
Irena Kruk ◽  
Beyza Torun ◽  
...  

Background: Stilbene phytalexis (1,2-diphenyloethylen) and benzamide are beneficial for human health. To increase the stilbene ring activity, a new series of its derivatives containing benzamide structure was synthesized and evaluated for their in vitro antioxidant power. Methods: 1H nuclear magnetic resonance, mass spectroscopy, and chromatographic analyses were used to confirm the successful synthesis. The antioxidant properties were determined by the elimination of , HO , DPPH , ABTS+ radicals, total antioxidant status (TAS) and the ferric reducing antioxidant activities (TAC) measurements. Results: Stilbenebenzamide compounds showed a wide spectrum of antioxidant ability, however their total antioxidant power was weaker than those of butylated hydroxytoluene (BHT), ascorbic acid, and resveratrol. The highest antiradical activity towards and HO was shown by the compounds with structures containing amine group (SBEBA, SBA) ( : 37.7 – 38.0% and 40.8 – 43.5%, HO : 29.8%, 28.7% inhibition, respectively) at1.25 mM concentration. The antiradical power of SBEBA (0.29) in DPPH assay was lower than those of resveratrol (1.83), ascorbic acid (3.63) and BHT (4.09). The TAS values of the synthesized compounds ranged from 152.9±5.3 to 240.2±6.7µM trolox equivalent/gram (TE/g) and were much lower than those of BHT (1304±43.0), reservatrol (1360±29.0) and ascorbic acid (2782±39.7) µM TE/g. Similarly, the TAC values ranging from 29.7±0.9 to 41.5±1.6 µM TE were weaker than that of resveratrol (239.2 ±6.7 µM TE/g). Conclusion: The results suggest that the presence of hydroxyl group in stilbene ring should be considered in further design of stilbenebenzamide compounds to enhance their antioxidant activity.


2013 ◽  
Vol 67 (2) ◽  
pp. 261-267 ◽  
Author(s):  
Milica Milutinovic ◽  
Slavica Siler-Marinkovic ◽  
Dusan Antonovic ◽  
Katarina Mihajlovska ◽  
Marija Pavlovic ◽  
...  

The importance of coffee waste utilization is based on the fact that it contains a large amount of biologically valuable components. Preparation of espresso coffee produces substantial quantities of polyphenolic acids that have a significant antioxidant activity. In this work, the contents of polyphenols and antioxidant activity of extracts obtained from spent espresso coffee were analyzed using different solvent systems. The 70% solution of methanol, and 70% solution of ethanol and distilled water were used as solvents. The total amounts of polyphenols were determined by the Folin-Ciocalteu method, while the antioxidant activities were determined by DPPH inhibition (2,2-diphenyl-1-picrylhydrazyl) and FRAP (Ferric Reducing Antioxidant Power). Both methods confirmed that the order of antioxidant activity of obtained dried extracts is as follows: water extract < methanol extract < ethanol extract, which was in accordance with their polyphenols content. Mild prooxidant activity was observed in the concentration of 200 ?g/ml while investigating the DPPH inhibition. Prooxidant activity is a characteristic of some polypenolic acids that is considered to be one of the mechanisms of anticancer activity. The inhibition of DPPH radical, expressed by IC50 values, was compared with the results of the commercial antioxidants such are ascorbic acid and BHT (butylated hydroxytoluene). Comparing the IC50 values of ascorbic acid and BHT with the IC50 values of tested extracts it can be concluded that waste coffee extracts could be a good source of natural antioxidants.


2013 ◽  
Vol 41 (1) ◽  
pp. 136 ◽  
Author(s):  
Violeta NOUR ◽  
Ion TRANDAFIR ◽  
Mira E. IONICA

Ten indeterminate tomato cultivars differing in fruit shape and typology, grown simultaneously in a greenhouse from Oltenia (Southwestern Romania) and harvested at red ripe stage, were investigated for the contents in ascorbic acid, lycopene, β-carotene, total phenolics, minerals (K, Na, Ca and Mg) and trace elements (Fe, Cu, Mn, Cr, Zn and B). In addition, their antioxidant activities were determined. Significant differences were found among tomato cultivars in all studied antioxidant compounds, as well as in the antioxidant activity. Ascorbic acid ranged from 91.9 to 329.7 mg kg-1 fw, lycopene ranged from 19.7 to 49.0 mg kg-1 fw, while total phenolic compounds varied between 300.2 and 557.8 mg kg-1 fw. Antioxidant activity ranged from 0.81 mmol Trolox kg-1 fw to 1.74 mmol Trolox kg-1 fw and it was significantly correlated to total phenolics content (r = 0.91; p< 0.05). The cocktail type cultivar ‘Tiger’ and the cherry type cultivar ‘Belle’ proved to be the most powerful in antioxidant activity and phenolic compounds while the rectangular plum shaped cultivar ‘Porto’ recorded the highest average lycopene content. A valuable cultivar proved to be the brownish red ‘Sacher’ which registered among the highest contents of phenolics, lycopene and ascorbic acid. Mineral and trace elements contents were also significantly affected by cultivars. Values recorded for K, Ca and Mg ranged from 2139.6 to 3056.9 mg kg-1, 137.7 to 325.8 mg kg-1 and 27.3 to 168.7 mg kg-1 respectively.


Author(s):  
M. Suleman Stephen ◽  
E. A. Adelakun ◽  
J. H. Kanus ◽  
Meshack M. Gideon

The presence of natural antioxidant in plants is well known. Plant phenolics constitute one of the major groups of components that act as antioxidant and free radical terminator. Hence, this study focused on investigating the antioxidant activity of Celery plant (Apium graveolens L). The fresh leaves were collected, crushed and extracted with ethanol and acetone by maceration. The radical scavenging properties of the extracts were determined by measuring changes in absorbance of DPPH radical at a wave lenght of 517 nm by UV and ascorbic acid is used as the standard. It showed that the crude ethanolic extract has higher antioxidant activity compared to ascorbic acid and acetone extract with less scavenging activity. The values were (IC50 114.6 µg/mL) for ascorbic acid, (IC50 112 µg/mL) for the crude ethanolic extract and (IC50172 µg/mL) for crude acetone extract. The result shows that Celery plant grown in Jos possess good antioxidant properties which may be linked to the presence of phenolics and flavonoids in the plant, which justifies its use as a medicinal plant. This can be further investigated for the isolation and identification of active compounds of medicinal utilities.


2010 ◽  
Vol 5 (2) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Gábor Janicsák ◽  
István Zupkó ◽  
Imre Máthé ◽  
Judit Hohmann

The lipid peroxidation-inhibiting activities of aqueous methanolic extracts of eleven Salvia species (Fam. Lamiaceae) were evaluated in an enzyme-independent biological system. The total polyphenol contents and the amounts of the most abundant phenoloids of the genus, caffeic and rosmarinic acids, were also determined. The EC50 values of the extracts displayed substantial differences. All of the investigated species except S. jurisicii (EC50 191.2 μg/mL) exhibited higher activities than that of ascorbic acid (EC50 123.8 μg/mL), the reference compound. Among the studied species, S. scabiosifolia (EC50 5.4 μg/mL) demonstrated the highest effect, followed in sequence by S. dumetorum, S. transsylvanica, S. officinalis l albiflora, S. nemorosa l albiflora and S. recognita (EC50 6.5 – 10.2 μg/mL). The close correlation was confirmed between the antioxidant activities and the total phenol contents of the extracts. For caffeic and rosmarinic acids, the correlation was much weaker, indicating the important role of other polyphenols in the antioxidant activity.


Agriculture ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 153 ◽  
Author(s):  
Ratna Suthar ◽  
Cun Wang ◽  
M. Nunes ◽  
Jianjun Chen ◽  
Steven Sargent ◽  
...  

As a soil amendment, biochar can significantly improve soil quality and crop growth. Few studies, however, have explored biochar effects on crop quality. This study investigated the amendment effects of bamboo biochar pyrolyzed at different temperatures on plant growth and fruit quality of tomato (Solanum lycopersicum L.). Tomato ‘Micro-Tom’ plants were grown in a sand medium amended with 0, 1, and 3% of biochars produced at 300 °C, 450 °C, and 600 °C, respectively. Plant growth was monitored, and fruit harvested at the red stage was analyzed for color, texture, soluble solids content, sugars, ascorbic acid, and acidity. Results showed that biochars produced at 300 °C and amended at 3% or pyrolyzed at 450 °C and amended at 1% increased plant growth index. Contents of glucose, fructose, soluble solids, ascorbic acid, and sugar-to-acid ratios of fruits produced from the two treatments were significantly higher than the other treatments. The improved plant growth and fruit quality were related to the higher concentrations of NO3, P, Ca, and Mg in the growing media. Our results suggest that optimizing biochar use can be achieved by targeting biochar production conditions and application rate, which resulted in desirable amendment and fruit quality effects.


Metabolites ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 183 ◽  
Author(s):  
Yong Sung Kwon ◽  
Sunmin Lee ◽  
Seung Hwa Lee ◽  
Hae Jin Kim ◽  
Choong Hwan Lee

Many ethnic fermented soybean products (FSPs) have long been consumed as seasoning and protein sources in East Asia. To evaluate the quality of various FSPs in East Asia, non-targeted metabolite profiling with multivariate analysis of six traditional FSPs (Natto; NT, Cheonggukjang; CG, Doenjang; DJ, Miso; MS, Doubanjiang; DB, Tianmianjiang; TM) was performed. Six FSPs could be clearly distinguished by principle component analysis (PCA) and partial least square-discriminant analysis (PLS-DA). Amino acid contents were relatively higher in NT and CG, sugar and sugar alcohol contents were relatively higher in MS and TM, isoflavone glycoside contents were relatively highest in CG, isoflavone aglycon contents were the highest in DJ, and soyasaponin contents were the highest in CG. Antioxidant activity and physicochemical properties were determined to examine the relationships between the FSPs and their antioxidant activities. We observed a negative correlation between isoflavone aglycon contents and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) activity. Furthermore, the order of ABTS activity of FSPs has a positive correlation with the order of soybean content in the six FSPs. Herein it was found that primary metabolites were affected by the main ingredients and secondary metabolites were most influenced by the fermentation time, and that soybean content contributed more to antioxidant activity than fermentation time.


Author(s):  
Resmi Mustarichie

Objective: The aim of this study is to investigate antioxidant activity and phytochemical screening of ethanol extract, fractions of water, ethyl acetate, and n-hexane from mistletoe tea (Scurrula atropurpurea Bl. Dans).Methods: Simplicia extracted using soxhlet equipment with 96% ethanol. Fractionation was conducted using liquid-liquid extraction using a solvent of water, ethyl acetate and n-hexane. Screening of phytochemical and antioxidant activity was performed against these fractions. Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl method using ultraviolet-visible spectrophotometry with ascorbic acid as standard. Phytochemical screening was conducted based on the method of Farnsworth.Results: The IC50 values of ethanol extract, water fraction, fraction of ethyl acetate, and n-hexane fraction were 21.92 ppm, 89.57 ppm, 14.08 ppm, and 162.09 ppm, respectively, whereas for ascorbic acid was 4.41 ppm. The ethanol extract and ethyl acetate fraction contained compounds were the same group, polyphenolic, tannins, flavonoids, monoterpenoid, steroids, triterpenoids, and quinones. Fraction of water contained compounds such as polyphenolic group, flavonoids, monoterpenoids, sesquiterpenoids, steroids, and triterpenoids. n-hexane fraction compounds contained steroids and triterpenoids.Conclusion: The ethanol extract, water fraction, ethyl acetate fraction, and n-hexane fraction showed antioxidant activities. The ethanol extract, fractions of water, and ethyl acetate fraction contained flavonoids and polyphenolic potential as antioxidants.Keywords: Mistletoe tea, Scurrula atropurpurea, Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Ascorbic acid.


2014 ◽  
Vol 67 (2) ◽  
pp. 99-108 ◽  
Author(s):  
Iwona Szot ◽  
Tomasz Lipa ◽  
Alina Basak

The chemical composition of strawberry fruit depends mainly on cultivar, fruit maturity degree and climatic conditions in the growing period. Fruit quality is also determined by a cultivation technology. The influence of two biostimulants (Atonik SL and Betokson Super 050 SL), which were used separately, in combination with each other or with the liquid fertilizer InsolCa, on yielding and fruit quality of two strawberry cultivars: ‘Senga Sengana’ and ‘Kent’, was tested. Betokson Super 050 SL + InsolCa tended to improve the yield of both cultivars. Significant differences compared to the control were evident especially in 2001. Fruits of ‘Senga Sengana’ started ripening later and had more ascorbic acid, anthocyanins and acidity than ‘Kent’. The use of Betokson Super 050 SL + InsolCa improved the content of ascorbic acid, anthocyanins and sugar as well as acidity in strawberries of both cultivars. The use of Betokson Super 050 SL + Atonik SL significantly increased the content of ascorbic acid and sugar in fruits of both cultivars. ‘Kent’ strawberries also responded to the treatment with an increase in anthocyanin content and acidity.


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