Immunohistochemical Localization And Monoclonal Antibodies Quantitative Measurement Of Carcinoembryonic Antigen (CEA) In Cervical Neoplasia

10.5580/fb0 ◽  
2003 ◽  
Vol 2 (1) ◽  
Keyword(s):  
Monoclonal Antibodies ◽  
Carcinoembryonic Antigen ◽  
Quantitative Measurement ◽  
Cervical Neoplasia ◽  
Immunohistochemical Localization

Conformational epitopes specific to carcinoembryonic antigen defined by monoclonal antibodies raised against colon cancer xenografts

1989 ◽  
Vol 42 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Yoichi Sakurai ◽  
Setsuo Hirohashi ◽  
Takashi Ohishi ◽  
Yukio Shimosato ◽  
Susumu Kodaira ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Monoclonal Antibodies ◽  
Carcinoembryonic Antigen ◽  
Conformational Epitopes

Immunohistochemical Localization of Cytochromes P450 with Polyclonal and Monoclonal Antibodies

1989 ◽  
Vol 8 (2) ◽  
pp. 61-94 ◽  
Author(s):  
Lucy M. Anderson ◽  
Jerrold M. Ward ◽  
Sang S. Park ◽  
Jerry M. Rice
Keyword(s):  
Monoclonal Antibodies ◽  
Cytochromes P450 ◽  
Immunohistochemical Localization

scholarly journals Preparation of Hybridomas Producing Monoclonal Antibodies against Aflatoxin B1 as a Tool to Control Hepatocellular Carcinoma

2019 ◽  
Vol 13 ◽  
pp. 1-12
Author(s):  
Manal M.E. Ahmed ◽  
Rafik Soliman ◽  
Jakeen Eljakee ◽  
Ahmed El-Sanousi ◽  
Haitham Amer ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Aflatoxin B1 ◽  
Quantitative Measurement ◽  
Myeloma Cell ◽  
Selective Medium ◽  
Immunization Schedule ◽  
Spleen Cells ◽  
Myeloma Cell Line ◽  
High Affinity ◽  
Multiple Uses

Hybridomas that secreted antibodies against aflatoxin B1 for multiple uses were prepared using a unique immunization schedule. Aflatoxin B1-BSA conjugate was used for immunization of Balb/c mice. Spleen cells were harvested from the hyper immunized mice to be fused with myeloma cell line (P3NS1) using polyethylene glycol 3000, 50% concentration as a fusogenic agent. The produced hybridomas were selected using HAT selective medium that was replaced by complete HT medium. From the 10thday after fusion, wells that contain colonies of hybridomas covering 30% or greater of the wells surface were screened for production of monoclonal antibodies against aflatoxin B1 using ELISA. 21 hybridomas were found to be reactive to aflatoxin B1. All were found to belong to IgG2aisotype except one was found to belong to IgM isotype. The prepared monoclonal antibodies and their application to immunoassays represents a useful and rapid quantitative measurement with high affinity and low detection limits in order to purify environmentally occurring levels of this carcinogen specially in areas at high risk for liver cancer.

Tumor Specificity of Monoclonal Antibodies to Carcinoembryonic Antigen

Tumor Biology ◽  
1990 ◽  
Vol 11 (1-2) ◽  
pp. 5-19 ◽  
Author(s):  
Fairouz Zoubir ◽  
Jan Zeromski ◽  
Jan Sikora ◽  
Jan Szmeja ◽  
Anders Hedin ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Carcinoembryonic Antigen ◽  
Tumor Specificity
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