HIV ile Enfekte Erkeklerde Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma spp. ve Mycoplasma spp. Varlığının Aranmasında İmmünokromatografik (IK) ve Gerçek Zamanlı Polimeraz Zincir Reaksiyonu (Rt-PCR) Testlerinin Kıyaslanması

2019 ◽  
Vol 53 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Pınar Zarakolu ◽  
Sıla Çetik ◽  
Ahmet Çağkan İnkaya ◽  
Serhat Ünal
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Rt Pcr ◽  
Ureaplasma Spp ◽  
Mycoplasma Spp

scholarly journals Monitoring Sexually Transmitted Infections in Cervicovaginal Exfoliative Samples in Mexican Women

Pathogens ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1618
Author(s):  
Fabiola Hernández-Rosas ◽  
Manuel Rey-Barrera ◽  
Ulises Conejo-Saucedo ◽  
Erika Orozco-Hernández ◽  
Liliana Maza-Sánchez ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Sexually Transmitted Infections ◽  
Trichomonas Vaginalis ◽  
Candida Spp ◽  
Sexually Transmitted ◽  
Multiplex Pcr Assay ◽  
Asymptomatic Patients ◽  
Ureaplasma Spp ◽  
Mycoplasma Spp

Background. Globally, Sexually Transmitted Infections (STIs) are a major cause of morbidity in sexually active individuals, having complications in reproduction health and quality of life. In concordance with the Sustainable Development Goals (SDG), the study aimed to investigate the prevalence of Candida spp., Ureaplasma spp., Trichomonas vaginalis, Neisseria gonorrhoeae, Chlamydia trachomatis, HSV, and Mycoplasma spp. from cervicovaginal samples and to correlate them with the gynecological history of the patients. Methods. Our analytical, prospective, and cross-sectional study included 377 women who participated in a reproductive health campaign during 2015–2016. Anthropometric and gynecological variables were obtained. Cervicovaginal specimens were collected and analyzed with a multiplex in-house PCR to detect Candida spp., Ureaplasma spp., Trichomonas vaginalis, Neisseria gonorrhoeae, HSV, Mycoplasma spp., and Chlamydia trachomatis. Results. The positive cases were 175/377 (46.4%) to at least one of the microorganisms. The most frequent pathogen detected in this population was Ureaplasma spp. (n = 111, 29.4%), followed by Mycoplasma spp. (n = 56, 14.9%) and Candida spp. (n = 47, 12.5%); 33.7% of the positive cases were single infections, whereas 12.7% had coinfection. The multiplex PCR assay was designed targeting nucleotide sequences. Conclusions. Our data demonstrated that monitoring STIs among asymptomatic patients will encourage target programs to be more precisely and effectively implemented, as well as make these programs more affordable, to benefit society by decreasing the prevalence of STIs.

scholarly journals Μοριακή επιδημιολογία και μικροβιολογία σεξουαλικώς μεταδιδόμενων νοσημάτων

2021 ◽  
Author(s):  
Χρήστος Παρθένης
Keyword(s):  
Chlamydia Trachomatis ◽  
Odds Ratio ◽  
Ureaplasma Spp ◽  
Mycoplasma Spp

Αντικείμενο Σκοπός: Η διερεύνηση και η διάγνωση των σεξουαλικώς μεταδιδόμενων νοσημάτων (ΣΜΝ) συμπεριλαμβανομένου και του HPV και η παρουσία κυτταρολογικών αλλαγών στον τράχηλο σε μια κοόρτη μελέτη με σεξουαλικώς ενεργές γυναίκες στον ελλαδικό χώρο.Υλικό και μέθοδοι: Πραγματοποιήθηκε κυτταρολογία τραχήλου μήτρας και μοριακή τυποποίηση HPV και άλλων ΣΜΝ για 345 ενεργά σεξουαλικά γυναίκες ηλικίας μεταξύ 18 και 45 ετών (μέσος όρος 33.2±7.2 έτη) που προσήλθαν σε γυναικολογική κλινική για έλεγχο διαλογής ρουτίνας. Μελετήθηκε η συσχέτιση του HPV και άλλων ΣΜΝ με την κυτταρολογική εικόνα.Αποτελέσματα: HPV εντοπίστηκε σε 61 γυναίκες (17.7%) και άλλα ΣΜΝ σε 82 (23.8%). Το Ureaplasma spp ήταν το πιο συχνά εντοπιζόμενο παθογόνο, το οποίο βρέθηκε σε 63 (18.2%) γυναίκες και ακολούθησαν το Mycoplasma spp (21 γυναίκες, 25.6%) και το Chlamydia trachomatis (πέντε γυναίκες, 6.1%). Η HPV θετικότητα μόνο (χωρίς τη συνύπαρξη άλλων ΣΜΝ) συσχετίσθηκε με μη φυσιολογική κυτταρολογία (odds ratio 6.9, p<0.001), ενώ γυναίκες που ήταν αρνητικές τόσο για HPV όσο και για τα άλλα ΣΜΝ είχαν μεγαλύτερη πιθανότητα να εμφανίσουν φυσιολογική κυτταρολογία τραχήλου μήτρας (odds ratio 0.36, p<0.01). Δεκαέξι από τις 63 (25.4%) γυναίκες οι οποίες ελέγχθηκαν θετικές για Ureaplasma spp, είχαν υψηλού ρίσκου HPV τύπο (odds ratio 2.3, p=0.02).Συμπεράσματα: Σε έναν πληθυσμό με αυξημένη εμφάνιση του Ureaplasma spp, υπήρχε συσχέτιση αυτού του συγκεκριμένου παθογόνου με υψηλού κινδύνου (υψηλού ρίσκου) HPV λοίμωξη, εύρημα που χρειάζεται περαιτέρω διερεύνηση και μελέτη.

scholarly journals No widespread dissemination of Chlamydia trachomatis diagnostic-escape variants and the impact of Neisseria gonorrhoeae positivity on the Aptima Combo 2 assay

2021 ◽  
pp. sextrans-2021-054988
Author(s):  
Michelle Jayne Cole ◽  
Grahame S Davis ◽  
Helen Fifer ◽  
John Michael Saunders ◽  
Magnus Unemo ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
False Negative ◽  
23S Rrna ◽  
Rrna Gene ◽  
Rt Pcr ◽  
New Variant ◽  
The Impact ◽  
Ct Dna ◽  
Widespread Dissemination

ObjectivesA Finnish Chlamydia trachomatis (CT) new variant was detected in 2019 that escaped detection in the Hologic Aptima Combo 2 (AC2) assay due to a C1515T mutation in the CT 23S rRNA target region. Reflex testing of CT-negative/CT-equivocal specimens as well as those positive for Neisseria gonorrhoeae (NG) with the Hologic Aptima CT (ACT) assay was recommended to identify any CT variants.MethodsFrom June to October 2019, specimens with discrepant AC2/ACT CT results were submitted to Public Health England and screened for detectable CT DNA using an inhouse real-time (RT)-PCR. When enough DNA was present, partial CT 23S rRNA gene sequencing was performed. Analysis of available relative light units and interpretative data was performed.ResultsA total of 317 discordant AC2/ACT specimens were collected from 315 patients. Three hundred were tested on the RT-PCR; 53.3% (n=160) were negative and 46.7% (n=140) were positive. Due to low DNA load in most specimens, sequencing was successful for only 36 specimens. The CT 23S rRNA wild-type sequence was present in 32 specimens, and two variants with C1514T or G1523A mutation were detected in four specimens from three patients. Of the discordant specimens with NG interpretation, 36.6% of NG-negative/CT-negative AC2 specimens had detectable CT DNA on the inhouse RT-PCR vs 53.3% of NG-positive/CT-negative specimens.ConclusionsNo widespread dissemination of AC2 diagnostic-escape CT variants has occurred in England. We however identified the impact of NG positivity on the discordant AC2/ACT specimens; a proportion appeared due to NG positivity and the associated NG signal, rather than any diagnostic-escape variants or low DNA load. Several patients with gonorrhoea may therefore receive false-negative AC2 CT results. Single diagnostic targets and multiplex diagnostic assays have their limitations such as providing selection pressure for escape mutants and potentially reduced sensitivity, respectively. These limitations must be considered when establishing diagnostic pathways.

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