scholarly journals Μοριακή επιδημιολογία και μικροβιολογία σεξουαλικώς μεταδιδόμενων νοσημάτων

2021 ◽  
Author(s):  
Χρήστος Παρθένης
Keyword(s):  
Chlamydia Trachomatis ◽  
Odds Ratio ◽  
Ureaplasma Spp ◽  
Mycoplasma Spp

Αντικείμενο Σκοπός: Η διερεύνηση και η διάγνωση των σεξουαλικώς μεταδιδόμενων νοσημάτων (ΣΜΝ) συμπεριλαμβανομένου και του HPV και η παρουσία κυτταρολογικών αλλαγών στον τράχηλο σε μια κοόρτη μελέτη με σεξουαλικώς ενεργές γυναίκες στον ελλαδικό χώρο.Υλικό και μέθοδοι: Πραγματοποιήθηκε κυτταρολογία τραχήλου μήτρας και μοριακή τυποποίηση HPV και άλλων ΣΜΝ για 345 ενεργά σεξουαλικά γυναίκες ηλικίας μεταξύ 18 και 45 ετών (μέσος όρος 33.2±7.2 έτη) που προσήλθαν σε γυναικολογική κλινική για έλεγχο διαλογής ρουτίνας. Μελετήθηκε η συσχέτιση του HPV και άλλων ΣΜΝ με την κυτταρολογική εικόνα.Αποτελέσματα: HPV εντοπίστηκε σε 61 γυναίκες (17.7%) και άλλα ΣΜΝ σε 82 (23.8%). Το Ureaplasma spp ήταν το πιο συχνά εντοπιζόμενο παθογόνο, το οποίο βρέθηκε σε 63 (18.2%) γυναίκες και ακολούθησαν το Mycoplasma spp (21 γυναίκες, 25.6%) και το Chlamydia trachomatis (πέντε γυναίκες, 6.1%). Η HPV θετικότητα μόνο (χωρίς τη συνύπαρξη άλλων ΣΜΝ) συσχετίσθηκε με μη φυσιολογική κυτταρολογία (odds ratio 6.9, p<0.001), ενώ γυναίκες που ήταν αρνητικές τόσο για HPV όσο και για τα άλλα ΣΜΝ είχαν μεγαλύτερη πιθανότητα να εμφανίσουν φυσιολογική κυτταρολογία τραχήλου μήτρας (odds ratio 0.36, p<0.01). Δεκαέξι από τις 63 (25.4%) γυναίκες οι οποίες ελέγχθηκαν θετικές για Ureaplasma spp, είχαν υψηλού ρίσκου HPV τύπο (odds ratio 2.3, p=0.02).Συμπεράσματα: Σε έναν πληθυσμό με αυξημένη εμφάνιση του Ureaplasma spp, υπήρχε συσχέτιση αυτού του συγκεκριμένου παθογόνου με υψηλού κινδύνου (υψηλού ρίσκου) HPV λοίμωξη, εύρημα που χρειάζεται περαιτέρω διερεύνηση και μελέτη.

scholarly journals Monitoring Sexually Transmitted Infections in Cervicovaginal Exfoliative Samples in Mexican Women

Pathogens ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1618
Author(s):  
Fabiola Hernández-Rosas ◽  
Manuel Rey-Barrera ◽  
Ulises Conejo-Saucedo ◽  
Erika Orozco-Hernández ◽  
Liliana Maza-Sánchez ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Sexually Transmitted Infections ◽  
Trichomonas Vaginalis ◽  
Candida Spp ◽  
Sexually Transmitted ◽  
Multiplex Pcr Assay ◽  
Asymptomatic Patients ◽  
Ureaplasma Spp ◽  
Mycoplasma Spp

Background. Globally, Sexually Transmitted Infections (STIs) are a major cause of morbidity in sexually active individuals, having complications in reproduction health and quality of life. In concordance with the Sustainable Development Goals (SDG), the study aimed to investigate the prevalence of Candida spp., Ureaplasma spp., Trichomonas vaginalis, Neisseria gonorrhoeae, Chlamydia trachomatis, HSV, and Mycoplasma spp. from cervicovaginal samples and to correlate them with the gynecological history of the patients. Methods. Our analytical, prospective, and cross-sectional study included 377 women who participated in a reproductive health campaign during 2015–2016. Anthropometric and gynecological variables were obtained. Cervicovaginal specimens were collected and analyzed with a multiplex in-house PCR to detect Candida spp., Ureaplasma spp., Trichomonas vaginalis, Neisseria gonorrhoeae, HSV, Mycoplasma spp., and Chlamydia trachomatis. Results. The positive cases were 175/377 (46.4%) to at least one of the microorganisms. The most frequent pathogen detected in this population was Ureaplasma spp. (n = 111, 29.4%), followed by Mycoplasma spp. (n = 56, 14.9%) and Candida spp. (n = 47, 12.5%); 33.7% of the positive cases were single infections, whereas 12.7% had coinfection. The multiplex PCR assay was designed targeting nucleotide sequences. Conclusions. Our data demonstrated that monitoring STIs among asymptomatic patients will encourage target programs to be more precisely and effectively implemented, as well as make these programs more affordable, to benefit society by decreasing the prevalence of STIs.

TRICHOMONAS VAGINALIS E ANORMALIDES CITOPATÓLOGICAS NOS EXAMES CITOPATOLÓGICOS REALIZADOS EM UM LABORATÓRIO CLÍNICO DA PONTIFÍCIA UNIVERSIDADE CATÓLICA DE GOIÁS - GO: ESTUDO DE PREVALÊNCIA

2020 ◽  
Vol 47 (1) ◽  
pp. 6104
Author(s):  
Andrea Alves Ribeiro ◽  
Sara Rosa Peixoto ◽  
Gabriel Henrique Martins de Almeida Araújo ◽  
Carolina Tonello dos Santos Pelá Alcanfor
Keyword(s):  
Herpes Simplex ◽  
Chlamydia Trachomatis ◽  
Odds Ratio ◽  
Trichomonas Vaginalis ◽  
Gardnerella Vaginalis ◽  
Candida Sp

O Trichomonas vaginalis é um protozoário que causa uma das principais infecções sexualmente transmissíveis. O objetivo foi estimar a prevalência de Trichomonas vaginalis em um Laboratório escola de Goiânia-GO. É um estudo retrospectivo utilizando as fichas de requisição dos exames citopatológicos realizados no Laboratório Clínico da Pontifícia Universidade Católica de Goiás (LC-PUCGO) entre janeiro de 2013 a dezembro de 2015. As variáveis foram categorizadas no programa Excel 2013 e tabulados no programa Epi Info™ versão 7, para avaliação da associação entre as variáveis foi realizado o cálculo do Odds Ratio (OR), considerando um intervalo de confiança (IC) de 95% e o valor de significância p< 0,05. Do total dos 4558 exames citopatológicos satisfatórios foi encontrada uma prevalência de Lactobacillus sp., de 46,97% (2141/4558) seguida por bacilos 24,62% (1122/4558), bacilos e cocos de 21,68% (988/4558), cocos de 3,86%(176/4558) e bacilos e lactobacilos de 2,87% (131/4558). Os agentes patogênicos encontrados foram Gardnerella vaginalis 79,6% (1141/1433), Candida sp. 17,7% (253/1433), Trichomonas vaginalis de 2,2% (32/1433), Herpes simplex de 0,4% (6/1433) e Chlamydia trachomatis de 0,1% (1/1433). Dentre os 4558 exames citopatológicos, 14,39% (656/4558) apresentaram anormalidades citológicas. As anormalidades encontradas foram ASC-US 2,57% (117/656), LSIL 1,78 % (81/656), ASC-H 3,52 % (160/656), HSIL 1,08% (48/656), AGC NEO 0,22% (10/656), Carcinoma invasor 0,02 % (1/656). Houve uma associação estatisticamente significante com a presença de Trichomonas vaginalis e o padrão anaeróbio OR 44,89 (IC 95 %: 10.5-271,8 p < 0,000). A detecção da manifestação por Trichomonas vaginalis é bastante sensível por meio do exame citopatológico. A presença de Trichomonas vaginalis, em um percentual relativamente baixo, indica a necessidade de identificação dos fatores de risco e aconselhamento em relação à proteção, transmissão e tratamento dessa infecção.

scholarly journals Role of Chlamydia trachomatis and emerging Chlamydia-related bacteria in ectopic pregnancy in Vietnam

2014 ◽  
Vol 143 (12) ◽  
pp. 2635-2638 ◽  
Author(s):  
S. HORNUNG ◽  
B. C. THUONG ◽  
J. GYGER ◽  
C. KEBBI-BEGHDADI ◽  
S. VASILEVSKY ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Odds Ratio ◽  
Case Control Study ◽  
Case Control ◽  
Molecular Evidence ◽  
High Seroprevalence ◽  
Independent Association ◽  
Ectopic Pregnancies ◽  
Control Study

SUMMARYIn this case-control study, we investigated the seroprevalence and molecular evidence of Chlamydia trachomatis and Waddlia chondrophila in ectopic pregnancies (EP) and uneventful control pregnancies in 343 women from Vietnam. Whereas presence of C. trachomatis IgG was strongly associated with EP [adjusted odds ratio (aOR) 5·41, 95% confidence interval (CI) 2·58–11·32], its DNA remained undetected in all tubal lesions. We confirmed an independent association between antibodies against Waddlia and previous miscarriage (aOR 1·87, 95% CI 1·02–3·42). Further investigations are needed to understand the clinical significance of Waddlia's high seroprevalence (25·9% in control pregnancies) in this urban population.

Mycoplasma and Chlamydia in Adenoids and Tonsils of Children Undergoing Adenoidectomy or Tonsillectomy

1994 ◽  
Vol 103 (2) ◽  
pp. 135-138 ◽  
Author(s):  
David Huminer ◽  
Rudi Levy ◽  
Silvio Pitlik ◽  
Zmira Samra
Keyword(s):  
Chlamydia Trachomatis ◽  
Chlamydial Infection ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Pathogenetic Role ◽  
Mycoplasma Spp ◽  
Genital Mycoplasmas

The prevalence of mycoplasmal and chlamydial infection was assessed in 83 children undergoing adenoidectomy, tonsillectomy, or both procedures for recurrent adenotonsillitis or obstructive symptoms. Throat smears (surface specimens) and minced adenoids and tonsils (core specimens) were cultured for Mycoplasma spp and for Chlamydia spp. Isolation rates in adenoidal specimens were as follows: Mycoplasma hominis, surface 7.1%, core 2.9%; and Ureaplasma urealyticum, surface 1.4%, core 2.9%. Mycoplasma hominis was also found in tonsillar specimens: surface 14.3%, core 20%. Chlamydia trachomatis was isolated only from a single core adenoidal specimen. The rate of mycoplasma isolation was significantly higher in children with recurrent adenotonsillitis (34.5%) than in those with obstructive symptoms (3.7%). Our findings document colonization of genital mycoplasmas in adenoids and tonsils of children with recurrent adenotonsillitis. Further studies are needed to evaluate the possible pathogenetic role of these microorganisms in adenotonsillar infection.

scholarly journals DISTRIBUTION OF MYCOPLASMA SPP. AND UREAPLASMA SPP. AMONG PREGNANT WOMEN

2021 ◽  
Vol 27 (1) ◽  
pp. 3630-3633
Author(s):  
Anton G. Petrov ◽  
◽  
Vladislav M. Nankov ◽  
Rositsa V. Petrova ◽  
Irena I. Gencheva ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Age Groups ◽  
University Hospital ◽  
Statistical Dependence ◽  
P Value ◽  
Age Group ◽  
Fetal Infection ◽  
Biological Methods ◽  
Ureaplasma Spp ◽  
Mycoplasma Spp

The purpose of the present study is to determine the prevalence of genital mycoplasmas (M. genitalium, M. hominis, U. parvum, U. urealyticum) in pregnant women by molecular biological methods. Material/Methods: A prospective epidemiological study of 107 pregnant women hospitalized in the Clinic of Obstetrics and Gynecology, University Hospital-Pleven, Bulgaria, was conducted. Vaginal secretion samples were taken from all 107 pregnant women. A Polymerase chain reaction (PCR) assay was used to detect the genomic DNA of the bacteria in pregnant women. Results: The highest is the relative share of women in the age group from 20 to 35 years - 66 (64.68%), followed by women under 20 years - 27 (25.23%) and women over 35 years - 14 (13.08%). Detection of bacterial DNA was found in 85 (79.44%) of the cases, with present Ureaplasma spp. Colonization in 42 women (39.25%). Although no statistical dependence was found on open bacteria and age groups (p-value = 0.4688), it is noteworthy that the prevalence of Mycoplasma spp. and Ureaplasma spp. as a whole in the age group from 20 to 35 years, which has the highest birth rate, is more than twice higher than the group of up to 20 years and more than five times higher compared to the group over 35 years. Conclusions: Studies on the incidence of Mycoplasma spp. and Ureaplasma spp. in pregnant women is important for controlling the pregnancy, predicting the risk of developing maternal-fetal infection and discussing the options for timely treatment.

scholarly journals HUBUNGAN INFEKSI Chlamydia trachomatis DENGAN KEJADIAN ABORTUS SPONTAN DI RSUD DR. RASIDIN DAN RSIA SITI HAWA PADANG

2018 ◽  
Vol 7 ◽  
pp. 15
Author(s):  
Wenny Nursa Octarina ◽  
Andani Eka Putra ◽  
Puja Agung Antonius
Keyword(s):  
Chlamydia Trachomatis ◽  
Odds Ratio ◽  
Case Control ◽  
Exact Test ◽  
Fisher's Exact Test ◽  
Consecutive Sampling ◽  
Fisher’S Exact Test

Abortus merupakan salah satu penyebab dari morbiditas dan mortalitas maternal. Abortus adalah berakhirnya kehamilan pada atau sebelum kehamilan tersebut berusia 22 minggu. Banyak faktor yang mempengaruhi abortus, salah satunya adalah infeksi yang disebabkan oleh Chlamydia trachomatis, selain itu faktor resiko lain yang mempengaruhi terjadinya abortus adalah usia maternal, paritas, riwayat kejadian abortus pada kehamilan sebelumnya, dan jarak kehamilan. Tujuan penelitian ini adalah untuk mengetahui hungan infeksi Chlamydia trachomatis dengan kejadian abortus spontan. Penelitian ini merupakan studi analitik dengan desain case control yaitu untuk mengetahui infeksi C. trachomatis dengan kejadian abortus spontan. Populasi penelitian adalah semua ibu hamil dengan abortus spontan di RS dr. Rasidin padang dan RSIA Siti Hawa Padang. Teknik pengambilan sampel dengan consecutive sampling yaitu setiap penderita yang memenuhi kriteria inklusi dimasukkan dalam subyek penelitian sampai jumlah sampel tercapai  yaitu sebanyak 50 sampel. Hasil penelitian didapatkan Proporsi kejadian abortus pada sampel positif C. trachomatis yaitu 44,0%. Analisa statistik dengan uji Fisher’s Exact Test menunjukan, terdapat hubungan yang bermakna antara infeksi C.trachomatis dengan kejadian abortus (p<0.05) dengan nilai Odds Ratio (OR) 5,7. Dari hasil penelitian dapat disimpulkan terdapat hubungan antara infeksi C.trachomatis dengan kejadian abortus. Diharapkan untuk penelitian berikutnya melakukan penelitian lebih lanjutan mengenai hubungan faktor resiko maternal dan  infeksi Chlamydia trachomatis  terhadap kejadian abortus spontan dengan metode penelitian yang berbeda dan jumlah sampel yang lebih banyak.

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