scholarly journals DNA Binding Properties of Two Ruthenium(III) Complexes Containing Schiff Bases Derived from Salicylaldehyde: Spectroscopic and Electrochemical Evidence of CT DNA Intercalation

2013 ◽  
Vol 86 (2) ◽  
pp. 215-222 ◽  
Author(s):  
Nevzeta Ljubijankić ◽  
Adnan Zahirović ◽  
Emir Turkušić ◽  
Emira Kahrović
Keyword(s):  
Dna Binding ◽  
Schiff Bases ◽  
Dna Intercalation ◽  
Binding Properties ◽  
Ct Dna ◽  
Dna Binding Properties

scholarly journals DNA binding properties, histidine interaction and cytotoxicity studies of water soluble ruthenium(ii) terpyridine complexes

2016 ◽  
Vol 45 (11) ◽  
pp. 4633-4646 ◽  
Author(s):  
Dejan Lazić ◽  
Aleksandar Arsenijević ◽  
Ralph Puchta ◽  
Živadin D. Bugarčić ◽  
Ana Rilak
Keyword(s):  
Dna Binding ◽  
Competitive Binding ◽  
Water Soluble ◽  
Binding Studies ◽  
Binding Properties ◽  
Cytotoxicity Studies ◽  
Vis Spectroscopy ◽  
Spectroscopy Studies ◽  
Ct Dna ◽  
Dna Binding Properties

UV-Vis spectroscopy studies, viscosity measurements and competitive binding studies with EB have revealed the ability of the complexes to bind to CT DNA covalently through N7 of guanine residues and non-covalently through intercalation.

Synthesis, Characterization, DNA Binding, and Photocleavage of [Ru(bpy)2(MFIP)]2+ and [Ru(phen)2(MFIP)]2+

2008 ◽  
Vol 61 (9) ◽  
pp. 725 ◽  
Author(s):  
Lifeng Tan ◽  
Sheng Zhang ◽  
Xiaohua Liu ◽  
Yue Xiao
Keyword(s):  
Mass Spectrometry ◽  
Dna Binding ◽  
1H Nmr Spectroscopy ◽  
Binding Properties ◽  
Experimental Conditions ◽  
Spectrophotometric Methods ◽  
Calf Thymus ◽  
Pbr322 Dna ◽  
Ct Dna ◽  
Dna Binding Properties

The new ligand 2-(5-methyl-furan-2-yl)imidazo[4,5-f][1, 10]phenanthroline (MFIP) and its complexes [Ru(bpy)2(MFIP)]2+ 1 (bpy = 2,2′-bipyridine) and [Ru(phen)2(MFIP)]2+ 2 (phen = 1,10-phenanthroline) were synthesized and characterized by elemental analysis, mass spectrometry, and 1H NMR spectroscopy. The DNA binding properties of the two complexes were investigated by different spectrophotometric methods and viscosity measurements. The results suggest that both complexes bind to calf thymus DNA (CT-DNA) through intercalation, and both complexes can enantioselectively interact with CT-DNA. The Λ enantiomers of both complexes are slightly predominant for binding to CT-DNA over the Δ enantiomer. When irradiated at 400 nm, the two complexes promote the photocleavage of pBR322 DNA, and complex 2 cleaves DNA more effectively than complex 1 under comparable experimental conditions. Furthermore, mechanism studies reveal that singlet oxygen (1O2) plays a significant role in the photocleavage.

DNA-Binding Properties of Iron(II) Mixed-Ligand Complexes Containing 1,10-Phenanthroline and Dipyrido[3,2-a:2’,3’-c]phenazine

2004 ◽  
Vol 59 (3) ◽  
pp. 310-318 ◽  
Author(s):  
◽  
Karna Wijaya ◽  
Daryono H. Tjahjono ◽  
Naoki Yoshioka ◽  
Hidenari Inoue
Keyword(s):  
Dna Binding ◽  
Bathochromic Shift ◽  
Mixed Ligand Complex ◽  
Visible Region ◽  
Spectrophotometric Titration ◽  
Mixed Ligand ◽  
Ligand Complex ◽  
Binding Properties ◽  
Ct Dna ◽  
Dna Binding Properties

An iron(II) mixed-ligand complex with 1,10-phenanthroline (phen) and dipyrido[3,2-a:2’,3’- c]phenazine (dppz), [Fe(phen)2(dppz)]2+, has been synthesized. The DNA-binding properties of the mixed-ligand complex have been studied in terms of equilibrium binding constant, thermodynamic parameter, thermal denaturation as well as Pfeiffer effect upon binding to DNA. The spectrophotometric titration of [Fe(phen)2(dppz)]2+ with calf thymus DNA (ct-DNA) has shown that the iron(II) mixed-ligand complex binds effectively to ct-DNA in an intercalation mode as indicated by remarkable hypochromicity (ca. 36%) and moderate bathochromic shift (8 nm) of the absorption spectra. This intercalative mode is supported by a significant increase (Δ Tm = 21 °C) in the melting temperature (Tm) of ct-DNA at R([complex]/[ct-DNA]) = 1.5. The binding of [Fe(phen)2(dppz)]2+ to ct-DNA is entropically driven as characterized by a positive enthalpy change and a large negative TΔ S term. An intense CD signal in the UV and visible region develops upon addition of ct-DNA to the racemate solution of [Fe(phen)2(dppz)]2+. This has revealed that a shift in diastereomeric inversion equilibrium takes place in the solution to yield an excess of one enantiomer of the DNA-iron(II) complex (Pfeiffer effect). The striking resemblance of the CD spectral profiles to those of the corresponding Δ -enantiomer indicates that Δ -[Fe(phen)2(dppz)]2+ is preferentially bound to ct-DNA

DNA-Binding and Photocleavage Behaviour of [Ru(bpy)2(MDPZ)]2+

2008 ◽  
Vol 61 (5) ◽  
pp. 376 ◽  
Author(s):  
Lifeng Tan ◽  
Hui Chao ◽  
Junjie Fei ◽  
Guojun Su ◽  
Sheng Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Nmr Spectroscopy ◽  
Dna Binding ◽  
1H Nmr Spectroscopy ◽  
Luminescent Probe ◽  
Binding Properties ◽  
Spectrophotometric Methods ◽  
Molecular Light Switch ◽  
Ct Dna ◽  
Dna Binding Properties

The new ligand MDPZ (MDPZ = 7,7′-methylenedioxyphenyldipyrido[3,2-a:2′,3′-c]-phenazine and its RuII complex [Ru(bpy)2(MDPZ)]2+ (phen = 1,10-phenanthroline) was synthesized and characterized by elemental analysis, mass spectrometry, and 1H NMR spectroscopy. The DNA-binding properties of [Ru(bpy)2(MDPZ)]2+ were investigated by different spectrophotometric methods and viscosity measurements. The results suggest that the complex binds to CT-DNA through intercalation, and can enantioselectively interact with CT-DNA. Although the [Ru(bpy)2(MDPZ)]2+ complex does not show the characteristics of a molecular ‘light switch’ for DNA, [Ru(phen)2(MDPZ)]2+ may serve as a sensitive luminescent probe for DNA. Also, when irradiated at 365 nm, the complex promotes the photocleavage of pBR 322 DNA.

scholarly journals Synthesis of 10-O-aryl-substituted berberine derivatives by Chan–Evans–Lam coupling and investigation of their DNA-binding properties

2021 ◽  
Vol 17 ◽  
pp. 991-1000
Author(s):  
Peter Jonas Wickhorst ◽  
Mathilda Blachnik ◽  
Denisa Lagumdzija ◽  
Heiko Ihmels
Keyword(s):  
Dna Binding ◽  
Coupling Reaction ◽  
Spectroscopic Studies ◽  
Binding Properties ◽  
Quadruplex Dna ◽  
G4 Dna ◽  
Fluorescence Light ◽  
Berberine Derivatives ◽  
Ct Dna ◽  
Dna Binding Properties

Eleven novel 10-O-aryl-substituted berberrubine and berberine derivatives were synthesized by the Cu2+-catalyzed Chan–Evans–Lam coupling of berberrubine with arylboronic acids and subsequent 9-O-methylation. The reaction is likely introduced by the Cu2+-induced demethylation of berberrubine and subsequent arylation of the resulting 10-oxyanion functionality. Thus, this synthetic route represents the first successful Cu-mediated coupling reaction of berberine substrates. The DNA-binding properties of the 10-O-arylberberine derivatives with duplex and quadruplex DNA were studied by thermal DNA denaturation experiments, spectrometric titrations as well as CD and LD spectroscopy. Fluorimetric DNA melting analysis with different types of quadruplex DNA revealed a moderate stabilization of the telomeric quadruplex-forming oligonucleotide sequence G3(TTAG3)3. The derivatives showed a moderate affinity towards quadruplex DNA (K b = 5–9 × 105 M−1) and ct DNA (K b = 3–5 × 104 M−1) and exhibited a fluorescence light-up effect upon complexation to both DNA forms, with slightly higher intensity in the presence of the quadruplex DNA. Furthermore, the CD- and LD-spectroscopic studies revealed that the title compounds intercalate into ct DNA and bind to G4-DNA by terminal stacking.

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