scholarly journals Assessment of efficacy and effectiveness of some extracted bio-chemicals as bio-fungicides on wood

2019 ◽  
Vol 70 (4) ◽  
pp. 337-350 ◽  
Author(s):  
Mohamed Z. M. Salem ◽  
Safa Abd El-Kader Mohamed Hamed ◽  
Maisa M. A. Mansour

The present study investigates in-vitro the antifungal activity of two extracts (ethyl ether extracts of Schinus terebinthifolius ripened fruits and Pinus rigida heartwood) and two essential oils (Thymus vulgaris and Origanum majorana leaves) against two species of fungi; Trichoderma harzianum and Aspergillus niger. The results clearly show that O. majorana oil and P. rigida wood extract had the highest activity against both fungi and were chosen for the application on four wood species; Weeping-Wreath Wattle (Acacia saligna), Beech (Fagus sylvatica), Black Walnut (Juglans nigra) and Pitch Pine (Pinus rigida). Additionally, their impact on the wood structure was examined by FTIR, SEM and colorimetry. The study suggests that O. majorana oil appears to show the best results and could be used as friendly bio-fungicides to protect wood objects without changing their structures.

2011 ◽  
Vol 13 (3) ◽  
pp. 342-348
Author(s):  
F. Rodrigues ◽  
H.H.C. Carvalho ◽  
J.M. Wiest

A partir da atividade antibacteriana in vitro, predeterminada em doze plantas com indicativo etnográfico condimentar, testou-se este atributo in loco no modelo caldo com frango cozido. Primeiramente, procedeu-se ao treinamento de 10 avaliadores, segundo a legislação vigente quanto ao Consentimento Livre e Esclarecido, oportunizando conhecimentos prévios sobre as plantas salsa (Petroselinum sativum), manjerona branca (Origanum X aplii), manjerona preta (Origanum majorana), manjericão (Ocimum basilicum), sálvia (Salvia officinalis), tomilho (Thymus vulgaris), anis verde (Ocimum selloi), alfavaca (Ocimum gratissimum), alho nirá (Allium tuberosum), alho poró (Allium porrum), cúrcuma (Curcuma longa) e pimenta dedo-de-moça (Capsicum baccatum). Realizou-se, através da adição individualizada desses condimentos ao caldo com frango cozido, um Teste de Aceitação tipo escala hedônica, selecionando, dentre os doze condimentos, quatro deles que se destacaram sensorialmente, a pimenta dedo-de-moça, o alho nirá, o alho poró e o tomilho. Foi feito, então, um Teste de Aceitação de concentrações denominadas pequena, média e grande destes quatro condimentos, para determinação da intensidade sensorialmente melhor aceita. As quantidades eleitas (0,5 g de pimenta dedo-de-moça, 15 g de alho nirá, 15 g de alho poró e 5 g de tomilho) foram acrescidas ao caldo com frango cozido, sendo estes desafiados frente a Escherichia coli (ATCC 11229) em concentração final de 10 UFC mL-1, limite tolerado pela legislação, tendo como grupo-controle o caldo com frango cozido sem condimentos. O crescimento bacteriano foi aferido a cada duas horas após a inoculação, até completar 24 horas de confronto, utilizando-se meio seletivo para coliformes termo-resistentes e incubação constante a 25ºC em DBO, sendo atribuídos valores arbitrários às variações logarítmicas de crescimento. Comparados ao controle, todos os tratamentos condimentados apresentaram, individualmente, atividade antibacteriana significativa, mesmo que sem significância quando comparados entre si. Contudo, em relação ao tempo de início da atividade antibacteriana, destacou-se a pimenta dedo-de-moça, enquanto que, em relação ao prolongamento dessa ação no tempo, destacou-se o alho nirá. As 12 plantas condimentares em estudo tiveram atestada a sensorialidade, sendo que as quatro plantas com destaque tiveram a atividade anti-coliforme termo-resistente comprovada in loco. Diferentes condimentos vegetais foram capazes de fornecer qualificação sensorial e sanitária em caldo com frango cozido, em condições domésticas de manuseio.


2010 ◽  
Vol 9 (2) ◽  
pp. 60
Author(s):  
J.S. Al-Hussaini, and A. M. G. Al-Mohana

The aim of this study was to evaluate the antifungal activity of the ethanolic extract of three local plants ( Elettaria cardamomum, Aloe vera, Thyme Vulgaris) against the growth of pathogenic Candida albicans in culture media. The antifungal activity was carried out by using agar well diffusion method. Ethanolic extracts of Elettaria cardamomum and Aloe vera inhibited the growth of Candida albicans isolates at all concentrations which tested in the present study (25, 50, 100, 150, 200, and 400) mg/ml, while the extract of Thymus vulgaris showed no activity against tested Candida albicans


2021 ◽  
Vol 28 ◽  
pp. 100452 ◽  
Author(s):  
Maryam Moazeni ◽  
Amirhossein Davari ◽  
Shafigheh Shabanzadeh ◽  
Javad Akhtari ◽  
Majid Saeedi ◽  
...  

Author(s):  
Christian Diehl ◽  
Natalia Reznichenko ◽  
Rodolfo Casero ◽  
Laura Faenza ◽  
Cecilia Cuffini ◽  
...  

Quassia amara is a plant of the family Simaroubaceae of Northern Brazilian origin.Its use in folk medicine is widespread, especially as an antiparasitic, antifungal and antibacterial agent. Our purpose was testing a Quassia amara ethanol wood extract (QWE) on various parasites, fungi and bacteria which had not been previously screened for this ingredient. QWE was found to have a strong antiparasitic effect on Demodex spp by counting the number of mites extracted from biopsies of pustules of patients with erythematotelangiectatic and papulopustular subtypes of rosacea along a topical treatment with 4% QWE, these numbers reaching their physiological value after a 42-day course. In Vitro testing of this extract on cultures of Trichomonas vaginalis collected from symptomatic patients showed a rapid inhibition of the growth of the trophozoites after 48 hours of contact. QWE also showed a marked antifungal activity on Candida spp (namely C. albicans, C. parapsilosis, C. glabrata and C. krusei, the latest at a lesser extent, and Malassezia furfur isolated from samples of infected patients, inhibiting the growth of fungi in both a time- and dose-dependent manner.The antibacterial activity of QWE was demonstrated in cultures of P. acnes and coagulase- positive Staphylococci where the growth of the bacteria was reduced in a significant manner (p<0.05) and at a lesser extent in cultures of coagulase-negative Staphylococci where the growth inhibition was not statistically significant. Contrarily, QWE had no effect on the growth of Chlamydia trachomatis, but uniquely altered the morphology and quantity of chlamydial inclusions.To the best of our knowledge, this is the first time that QWE is shown to have antiparasitic activity on Trichomonas vaginalis and Demodex spp, an antifungal activity on Malassezia furfur and Candida spp and an antibacterial activity on P. acnes


2008 ◽  
Vol 54 (11) ◽  
pp. 950-956 ◽  
Author(s):  
Patrícia Pozzatti ◽  
Liliane Alves Scheid ◽  
Tatiana Borba Spader ◽  
Margareth Linde Atayde ◽  
Janio Morais Santurio ◽  
...  

In the present study, the antifungal activity of selected essential oils obtained from plants used as spices was evaluated against both fluconazole-resistant and fluconazole-susceptible Candida spp. The Candida species studied were Candida albicans , Candida dubliniensis , Candida tropicalis , Candida glabrata , and Candida krusei. For comparison purposes, they were arranged in groups as C. albicans, C. dubliniensis, and Candida non-albicans. The essential oils were obtained from Cinnamomum zeylanicum Breyn, Lippia graveolens HBK, Ocimum basilicum L., Origanum vulgare L., Rosmarinus officinalis L., Salvia officinalis L., Thymus vulgaris L., and Zingiber officinale . The susceptibility tests were based on the M27-A2 methodology. The chemical composition of the essential oils was obtained by gas chromatography – mass spectroscopy and by retention indices. The results showed that cinnamon, Mexican oregano, oregano, thyme, and ginger essential oils have different levels of antifungal activity. Oregano and ginger essential oils were found to be the most and the least efficient, respectively. The main finding was that the susceptibilities of fluconazole-resistant C. albicans, C. dubliniensis, and Candida non-albicans to Mexican oregano, oregano, thyme, and ginger essential oils were higher than those of the fluconazole-susceptible yeasts (P < 0.05). In contrast, fluconazole-resistant C. albicans and Candida non-albicans were less susceptible to cinnamon essential oil than their fluconazole-susceptible counterparts (P < 0.05). A relationship between the yeasts’ susceptibilities and the chemical composition of the essential oils studied was apparent when these 2 parameters were compared. Finally, basil, rosemary, and sage essential oils did not show antifungal activity against Candida isolates at the tested concentrations.


2020 ◽  
Vol 18 (1) ◽  
pp. 108-118 ◽  
Author(s):  
Tomasz Baj ◽  
Anna Biernasiuk ◽  
Rafał Wróbel ◽  
Anna Malm

AbstractThe purpose of this research was to investigate the chemical composition of essential oils (EOs) from: Origanum vulgare L., Satureja hortensis L., Thymus serpyllum L. and Thymus vulgaris L. (Lamiaceae) cultivated in Poland, and to study their antifungal activity towards clinical isolates of oral Candida spp. The hydrodistilled essential oils were analyzed using the GC-MS method. The antifungal activity was evaluated in vitro against oral isolates and reference strains of Candida albicans and C. glabrata, using the broth microdilution method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) and Clinical and Laboratory Standards Institute (CLSI) guidelines, allowing for estimation of minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC). GC-MS analysis revealed that carvacrol was the main EO compound in oregano and summer savory, while thymol and linalool were the major ingredients of thyme and wild thyme oils, respectively. The EOs possessed fungicidal activity against C. albicans and C. glabrata, including oral isolates, with MIC = 125 – 2000 mg/L, MFC = 250 – 4000 mg/L and MFC/MIC = 1 – 4, depending on the yeast and plant species. The most active was thyme oil – with MIC = 125 – 500 mg/L, MFC = 250 – 500 mg/L and MFC/MIC = 1 – 2.


2020 ◽  
Vol 88 (3) ◽  
pp. 33 ◽  
Author(s):  
Mohamed Nadjib Boukhatem ◽  
Noureldien H. E. Darwish ◽  
Thangirala Sudha ◽  
Siham Bahlouli ◽  
Dahbia Kellou ◽  
...  

The aim of this study is to investigate the Thymus vulgaris essential oil (TVEO) as an antifungal agent in aromatherapy and/or as an active ingredient in the prevention or management of topical inflammatory diseases. The chemical composition of TVEO was determined with gas chromatography and revealed the presence of 25 compounds. Carvacrol was found to be the major component (56.8%). Antifungal action of TVEO was determined in vitro by using different methods. By the disc diffusion method, TVEO showed more potent antifungal activity against Candida strains than the positive control. The diameter of inhibition zone (DIZ) varied from 34 to 60 mm for Candida yeasts. Significantly higher antifungal activity was observed in the vapor phase at lower quantities. Candida albicans and C. parapsilosis were the most susceptible strains to the oil vapor with DIZ varying from 35 to 90 mm. The minimum inhibitory concentrations (MIC) of yeast were determined with an agar dilution method and revealed that MIC varied from 0.3 to 0.15 µL/mL for yeast species. The topical anti-inflammatory potential of TVEO was also explored in vivo with the croton oil-induced ear edema assay. TVEO exhibited a potent anti-inflammatory effect at all doses (100, 10 and 2 mg/kg), which were statistically similar (p > 0.05) to the positive control. This activity was also confirmed at the cellular level with histopathology analysis. Our results suggest the potential application of this carvacrol-rich TVEO in the prevention and management of fungal infections and topical inflammation and deserve further investigation for clinical applications. Furthermore, while the mode of action remains mainly undetermined and should be studied.


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