scholarly journals Aryl Hydrocarbon Receptor Mediates Indoxyl Sulfate-Induced Cellular Senescence in Human Umbilical Vein Endothelial Cells

2014 ◽  
Vol 21 (9) ◽  
pp. 904-916 ◽  
Author(s):  
Masayuki Koizumi ◽  
Junko Tatebe ◽  
Ippei Watanabe ◽  
Junichi Yamazaki ◽  
Takanori Ikeda ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Aryl Hydrocarbon Receptor ◽  
Cellular Senescence ◽  
Umbilical Vein ◽  
Indoxyl Sulfate ◽  
Human Umbilical Vein ◽  
Aryl Hydrocarbon ◽  
Umbilical Vein Endothelial Cells

Fructated apolipoprotein A-I exacerbates cellular senescence in human umbilical vein endothelial cells accompanied by impaired insulin secretion activity and embryo toxicity

2016 ◽  
Vol 94 (4) ◽  
pp. 337-345 ◽  
Author(s):  
Ki-Hoon Park ◽  
Jae-Yong Kim ◽  
Inho Choi ◽  
Jae-Ryong Kim ◽  
Kyu Chang Won ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Insulin Secretion ◽  
Cellular Senescence ◽  
Metabolic Diseases ◽  
Umbilical Vein ◽  
Density Lipoprotein ◽  
Human Umbilical Vein ◽  
Lysine Residues ◽  
Artery Disease ◽  
Umbilical Vein Endothelial Cells

Glycation of apolipoproteins is a major feature of the production of dysfunctional high-density lipoprotein (HDL), which is associated with the incidence of several metabolic diseases such as coronary artery disease and diabetes. In this report, fructated apoA-I (fA-I) induced by fructose treatment showed a covalently multimerized band without cross-linking, and lysine residues were irreversibly modified to prevent crosslinking. Using pancreatic β-cells, insulin secretion was impaired by fA-I in the lipid-free and reconstituted HDL (rHDL) states, by up to 35%, and 40%, respectively, under hyperglycemic conditions (25 mmol/L glucose). Treatment of human umbilical vein endothelial cells (HUVECs) with fA-I and HDL from elderly patients caused a 1.8-fold and 1.5-fold increased cellular senescence, respectively, along with increased lysosomal enlargement. In the lipid-free and rHDL states, fA-I increased embryo death by 1.5-fold and 2.5-fold, respectively, along with the production of oxidized species. Furthermore, rHDL containing fA-I (fA-I-rHDL) showed a higher isoelectric point (pI, approximately 8.5), whereas rHDL containing nA-I (nA-I-rHDL) showed a narrow band range with lower pI (around 8.0) as well as a much smaller particle size than that of nA-I-rHDL. In conclusion, fructose-mediated apoA-I fructation resulted in the severe loss of several beneficial functions of apoA-I and HDL, including anti-senescence and insulin secretion activities, accompanied with increased susceptibility to protein degradation and structural modification.

Indoxyl sulfate accelerates cellular senescence through aryl hydrocarbon receptor in endothelial cells

2015 ◽  
Vol 9 (4) ◽  
pp. e10
Author(s):  
Toshisuke Morita ◽  
Junko Tatebe ◽  
Ippei Watanabe ◽  
Masayuki Koizumi ◽  
Takanori Ikeda
Keyword(s):  
Endothelial Cells ◽  
Aryl Hydrocarbon Receptor ◽  
Cellular Senescence ◽  
Indoxyl Sulfate ◽  
Aryl Hydrocarbon
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