scholarly journals An aryl hydrocarbon receptor agonist suppresses the growth of human umbilical vein endothelial cells in vitro: Potent effect with polyunsaturated fatty acids

2020 ◽  
Vol 101 (6) ◽  
pp. 248-263
Author(s):  
Masayoshi Yamaguchi ◽  
Oliver Hankinson
Keyword(s):  
Fatty Acids ◽  
Endothelial Cells ◽  
Aryl Hydrocarbon Receptor ◽  
Receptor Agonist ◽  
Umbilical Vein ◽  
Potent Effect ◽  
Aryl Hydrocarbon ◽  
Aryl Hydrocarbon Receptor Agonist ◽  
Umbilical Vein Endothelial Cells

ω-3 Fatty acids suppress monocyte adhesion to human endothelial cells: role of endothelial PAF generation

2002 ◽  
Vol 283 (2) ◽  
pp. H811-H818 ◽  
Author(s):  
Konstantin Mayer ◽  
Martina Merfels ◽  
Marion Muhly-Reinholz ◽  
Stephanie Gokorsch ◽  
Simone Rosseau ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Endothelial Cells ◽  
Adhesion Molecule ◽  
Inflammatory Diseases ◽  
Umbilical Vein ◽  
Platelet Activating Factor ◽  
Surface Expression ◽  
Intercellular Adhesion Molecule ◽  
Umbilical Vein Endothelial Cells

Monocyte-endothelium interaction is a fundamental process in many acute and chronic inflammatory diseases. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are fish oil-derived alternative (ω-3) precursor fatty acids implicated in the suppression of inflammatory events. We investigated their influence on rolling and adhesion of monocytes to human umbilical vein endothelial cells (HUVEC) under laminar flow conditions in vitro. Exposure of HUVEC to tumor necrosis factor (TNF-α) strongly increased 1) surface expression of intercellular adhesion molecule (ICAM-1), vascular cell adhesion molecule (VCAM-1), and E-selectin, 2) platelet-activating factor (PAF) synthesis as assessed by thrombin challenge, and 3) rate of rolling and adhesion of monocytes. Preincubation of HUVEC with EPA or DHA markedly suppressed PAF synthesis, monocyte rolling, and adherence, whereas expression of endothelial adhesion molecules was unchanged. Also, PAF receptor antagonists markedly suppressed the adhesion rate of monocytes, and EPA or DHA revealed no additional inhibitory capacity. In contrast, arachidonic acid partially reversed the effect of the antagonist. We conclude that ω-3 fatty acids suppress rolling and adherence of monocytes on activated endothelial cells in vitro by affecting endothelial PAF generation.

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