scholarly journals Antimicrobial Effects of Sulfadimethoxine on Salmonella, Escherichia coli and Aerobic plate count (APC) in Small-Scale Broiler Operations

2019 ◽  
Vol 8 (6) ◽  
pp. 147
Author(s):  
Wannee Tangkham ◽  
Frederick LeMieux
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Antibiotic Treatment ◽  
Experimental Treatment ◽  
Control Treatment ◽  
Plate Count ◽  
Small Scale ◽  
Antimicrobial Effects ◽  
E Coli ◽  
Aerobic Plate Count

Poultry is a source of Salmonella and Escherichia coli. Antibiotics can be used to reduce the enumeration and prevalence of these bacteria. The objective of this study was to determine the effects of sulfadimethoxine antibiotic on the enumeration and prevalence of Salmonella, Escherichia coli and aerobic plate count in broilers. Broilers (n = 600) were allotted to two treatments, each with twelve replications. The treatments were control (drinking water without antibiotic) and with antibiotic at 0.05% (wt/vol) sulfadimethoxine. After a six-week period, the enumeration of Salmonella in the experimental treatment was detected at 2.55 log CFU/g. This value is not different (P>0.05) than that detected in the control at 2.81 log CFU/g. With respect to prevalence, there was a difference (P<0.05) between the experimental treatment at 90.0% and the control at 100%. The enumeration of E. coli in the experimental treatment was detected at 3.97 log CFU/g. This value is lower (P<0.05) than that detected in the control treatment at 4.37 log CFU/g. With respect to prevalence, there was no difference (P>0.05) between the experimental treatment at 100% and the control at 100%. The enumeration of aerobic plate count in the antibiotic treatment were detected at 6.62 log CFU/g. This value is lower (P<0.05) than that detected in the control at 7.50 log CFU/g. With respect to prevalence, there was no difference (P>0.05) between treatments. Our overall findings suggest that the use of the antibiotic sulfadimethoxine can reduce the number of E. coli, Salmonella and aerobic plate count in the small-scale broiler operations.

Contamination Revealed by Indicator Microorganism Levels during Veal Processing

2016 ◽  
Vol 79 (8) ◽  
pp. 1341-1347 ◽  
Author(s):  
JOSEPH M. BOSILEVAC ◽  
RONG WANG ◽  
BRANDON E. LUEDTKE ◽  
TOMMY L. WHEELER ◽  
MOHAMMAD KOOHMARAIE
Keyword(s):  
Escherichia Coli ◽  
Plate Count ◽  
Department Of Agriculture ◽  
E Coli ◽  
Veal Calves ◽  
Indicator Microorganism ◽  
Analysis Of Results ◽  
Aerobic Plate Count ◽  
Inspection Service ◽  
The U.S

ABSTRACT During site visits of veal processors, the U.S. Department of Agriculture, Food Safety Inspection Service (FSIS) has reported processing deficiencies that likely contribute to increased levels of veal contamination. Here, we report the results of measuring aerobic plate count bacteria (APC), Enterobacteriaceae, coliforms (CF), and Escherichia coli during eight sample collections at five veal processors to assess contamination during the harvest of bob veal and formula-fed veal before (n = 5 plants) and after (n = 3 plants) changes to interventions and processing practices. Hides of veal calves at each plant had mean log CFU/100 cm2 APC, Enterobacteriaceae, CF, and E. coli of 6.02 to 8.07, 2.95 to 5.24, 3.28 to 5.83, and 3.08 to 5.59, respectively. Preintervention carcasses had mean log CFU/100 cm2 APC, Enterobacteriaceae, CF, and E. coli of 3.08 to 5.22, 1.16 to 3.47, 0.21 to 3.06, and −0.07 to 3.10, respectively, before and 2.72 to 4.50, 0.99 to 2.76, 0.69 to 2.26, and 0.33 to 2.12, respectively, after changes were made to improve sanitary dressing procedures. Final veal carcasses had mean log CFU/100 cm2 APC, Enterobacteriaceae, CF, and E. coli of 0.36 to 2.84, −0.21 to 1.59, −0.23 to 1.59, and −0.38 to 1.45 before and 0.44 to 2.64, −0.16 to 1.33, −0.42 to 1.20, and −0.48 to 1.09 after changes were made to improve carcass-directed interventions. Whereas the improved dressing procedures resulted in improved carcass cleanliness, the changes to carcass-directed interventions were less successful, and veal processors are urged to use techniques that ensure uniform and consistent delivery of antimicrobials to carcasses. Analysis of results comparing bob veal to formula-fed veal found bob veal hides, preintervention carcasses, and final carcasses to have increased (P < 0.05) APC, Enterobacteriaceae, CF, and E. coli (with the exception of hide Enterobacteriaceae; P > 0.05) relative to formula fed veal. When both veal categories were harvested at the same plant on the same day, similar results were observed. Since identification by FSIS, the control of contamination during veal processing has started to improve, but challenges still persist.

scholarly journals Evaluation of microbial quality and pathogenic potentiality of enterobacteria in poultry feeds

2019 ◽  
Vol 28 ◽  
pp. 59-68
Author(s):  
S Islam ◽  
N Tanjila ◽  
MF Begum
Keyword(s):  
Escherichia Coli ◽  
Morphological Characteristics ◽  
Coliform Bacteria ◽  
Poultry Feed ◽  
Plate Count ◽  
Test Results ◽  
Poultry Feeds ◽  
E Coli ◽  
Aerobic Plate Count ◽  
Feed Samples

The present investigation has been carried out to assess the microbial safety and pathogenic potentialities of enterobacteria in poultry feeds. From the results it was observed that total aerobic plate count of poultry feeds samples were recorded as 2.8 × 105 to 5.8 × 109 cfu/g and 100% samples contained ≥106 cfu/g while the highest mean of cfu was counted as log10 8.797/gm. Large number of coliforms were recorded in different poultry feed samples and the ranges of cfu were counted as 1.2 × 104 to 5.2 × 107/g while average 75% samples were contaminated with coliform bacteria with ≥104 cfu/g and the highest mean of cfu was counted as log10 6.103/g. The ranges of cfu of Escherichia coli were 1.03 × 102 to 1.09 × 105/g and 70% samples contained ≥102 cfu/g while the highest mean of cfu was counted as log10 4.493/gm. But the ranges of cfu of total Salmonella sp. were recorded as 1.02 × 101 to 5.25 × 104/g and 50% samples contained ≥102 cfu/g and the highest mean of cfu was counted as log10 3.665/g. Total 29 enterobacterial isolates were isolated from the feed by using selected media. On the basis of morphological characteristics and biochemical test results the isolates were identified as Salmonella sp., Shigella sp., Klebsiella sp., Citrobacter sp., Proteus sp., Enterobacter sp. and Escherichia coli. These isolates were tested on blood agar medium and only seven isolates showed positive β-hemolytic activity.In virulence efficacy test, only hemolytic positive isolates were ingested to chicken and observed that E. coli (SGE-1), Klebsiella sp. (SSE-6) and Salmonella sp. (JSS-9) isolates were highly toxic because the experimental chickens were died after 3 days of ingestion of the bacteria, two isolates showed loose motion symptom after 15 days while other isolates showed little sickness. All the selected isolates showed positive hem-agglutination reactivity in poultry RBC. The results indicate that the poultry feeds were highly contaminated with pathogenic enterobacteria which are risk to public health. J. bio-sci. 28: 59-68, 2020

Comparison of Brands of Media for Isolating Bacteria from Poultry, Beef and Shrimp

1984 ◽  
Vol 47 (5) ◽  
pp. 394-397 ◽  
Author(s):  
H. S. LILLARD ◽  
N. A. COX ◽  
J. S. BAILEY ◽  
J. E. THOMSON
Keyword(s):  
Escherichia Coli ◽  
Practical Significance ◽  
Plate Count ◽  
E Coli ◽  
Plate Counts ◽  
Aerobic Plate Count ◽  
Raw Shrimp

Five brands of media (BBL, Difco, Gibco, Oxoid and Scott) were evaluated for enumerating microorganisms by the aerobic plate count and by Enterobacteriaceae, Escherichia coli, and coliform counts, and for determining Salmonella incidence. Microbiological evaluations were done on raw chickens, raw beef and raw shrimp, except that Salmonella incidence was not determined on shrimp samples. There were statistically significant differences in total plate counts (with chicken, beef and shrimp), Enterobacteriaceae counts (with shrimp) coliforms (with chicken) and E. coli counts (with chicken) by the five brands of media, but these differences were too small to be of practical significance. It was concluded that no differences of practical significance were found among the five brands of media.

Fate of Enterohemorrhagic Escherichia coli O157: H7 in Commercial Mayonnaise

1994 ◽  
Vol 57 (9) ◽  
pp. 780-783 ◽  
Author(s):  
TONG ZHAO ◽  
MICHAEL P. DOYLE
Keyword(s):  
Escherichia Coli ◽  
Enterohemorrhagic Escherichia Coli ◽  
Health Concern ◽  
Plate Count ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Initial Ph ◽  
Contaminated Food ◽  
Aerobic Plate Count ◽  
Mold And Yeast

The fate of enterohemorrhagic Escherichia coli O157:H7 was determined in three different lots of commercial mayonnaise, including four different samples from a lot implicated in an outbreak of E. coli O157:H7 infection. The initial pH of the products ranged from 3.6 to 3.9. Products were inoculated with 6.5 × 103 E. coli O157:H7/g and incubated at 5 or 20°C. Escherichia coli O157:H7 did not grow at either temperature but survived for 34 to 55 days at 5°C and for 8 to 21 days at 20°C, depending on the lot. Survival was greatest in real mayonnaise purchased at retail among six mayonnaise samples which included a reduced calorie mayonnaise. Escherichia coli O157:H7 populations decreased between 2- and 100-fold by 3 weeks at 5°C, and between 10- and 1,000-fold by 7 days at 20°C. There was little or no change in pH (<0.1 unit), aerobic plate count, mold and yeast count or Lactobacillus count (< 1 log10 CFU/g) for the duration of the study. Commercial mayonnaise manufactured under good manufacturing practices is not a public health concern. Abusive handling of mayonnaise resulting in cross-contamination with E. coli O157:H7-contaminated food or contamination by an infected foodhandler is the principal basis for concern.

scholarly journals PENGARUH PENCELUPAN KARKAS AYAM PEDAGING DALAM LARUTAN ASAM SITRAT DAN ASAM ASETAT TERHADAP ANGKA LEMPENG TOTAL Escherichia coli (The Immersion Effect of Broiler Carcass in Citric Acid and Acetic Acid on the Total Plates Count of Escherichia Coli)

2015 ◽  
Vol 9 (2) ◽  
Author(s):  
Nurliana Nurliana ◽  
Sandy Cakra Yuda ◽  
Faisal Jamin ◽  
Teuku Reza Ferasyi ◽  
M. Isa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Acetic Acid ◽  
Citric Acid ◽  
Acid Solution ◽  
Observation Time ◽  
Chicken Meat ◽  
Control Treatment ◽  
Plate Count ◽  
E Coli ◽  
Chicken Carcass

This study aimed to determine the immersion effect of broiler carcasses in 3% citric acid, 3% acetic acid, and combination of citric acid and acetic acid with concentration of 1.5% each on the total plate count of bacteria E. coli. Eight broilers carcasses (dissected into 2 parts) were divided into 4 treatment groups with 2 times repetition and 3 times observation at 0, 4, and 8 hours after dipped. The control treatment (K0) without dipped into acid solution. Broiler carcasses in the first (K1), second (K2), and the third (K3) treatments were dipped into 3% acetic acid, 3% citric acid, and combination of 1.5% acetic acid and 1.5% citric acid respectively. Data were analyzed using analysis of variance. The average of E. coli count in K0 at 0, 4, and 8 hours post dipping were 5.2±0.061, 6.8±0.99, and 7.0±0.107 log 10 cfu/g of chicken meat respectively. In similar observation time with K0, the average of total E. coli count were 5.5±0.373, 6.0±0.023, and 6.0±0.242 log 10 cfu/g of chicken meat in K1, 5.3±0.166, 6.0±0.143, and 6.0±0.084 log 10 cfu/g of chicken meat in K2, 5.7±0.041, 5.9±0.92, and 5.8±0.058 log 10 cfu/g of chicken meat in K3 at 0,4, and 8 hours post dipping respectively. Statistical analysis showed that the immersion of broiler carcass in acetic acid and citric acid solution were significantly (P <0.05) decrease the number of E. coli. There were interaction between immersion of broiler carcass in organic acids and observation time which significantly decrease the number of E. coli (p <0.05). In conclusion, the immersion of carcass chicken in 3 % acetic acid, 3% citric acid, and their combination reduce the amount of E. coli. Key words: chicken carcass , citric acid 3%, acetic acid 3% , Escherichia coli

The Aerobic Plate Count, Coliform and Escherichia coli Content of Raw Ground Beef at the Retail Level

1976 ◽  
Vol 39 (3) ◽  
pp. 175-178 ◽  
Author(s):  
J. M. GOEPFERT
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
New York ◽  
Ground Beef ◽  
The United States ◽  
Plate Count ◽  
Raw Meat ◽  
E Coli ◽  
Step Procedure ◽  
Aerobic Plate Count

Nine hundred fifty-five samples of raw ground beef obtained from supermarkets throughout the United States were examined for coliforms, Escherichia coli, and Aerobic Plate Count (APC). The results were compared with existent standards for E. coli in raw meat in New York and Oregon. Lack of homogenious distribution of E. coli in fresh ground beef was demonstrated. Observations were made that indicate that a 2 day-two step procedure will detect the same number of E. coli as the more time consuming four step MPN procedure 98% of the time. A comparison of the APC obtained by incubating plates at 20 C and 35 C showed there to be an average 10-fold difference with the 20 C incubation always higher. Questions are raised about the necessity of microbial standards for raw meat and the validity of incorporating E. coli in such standards.

Assessment of Microbiological Safety and Quality of Marinades Used To Treat Beef and That Were Collected over a 12-Month Period from Specialty Retailers Near Raleigh, North Carolina

2018 ◽  
Vol 81 (3) ◽  
pp. 490-496 ◽  
Author(s):  
Yangjin Jung ◽  
Christopher L. Rupert ◽  
Benjamin Chapman ◽  
Anna C. S. Porto Fett ◽  
John B. Luchansky
Keyword(s):  
Escherichia Coli ◽  
North Carolina ◽  
Real Time ◽  
Shiga Toxin ◽  
Real Time Pcr ◽  
Plate Count ◽  
Pcr Assay ◽  
E Coli ◽  
Aerobic Plate Count

ABSTRACT In total, 115 marinade samples (58 fresh marinades and 57 spent marinades) were collected over 12 months from specialty retailers (four individual stores) near Raleigh, NC. These marinades were screened for total mesophilic aerobic plate count (M-APC), total psychrotrophic aerobic plate count (P-APC), and Enterobacteriaceae. These marinades were also screened for the seven regulated serogroups of Shiga toxin–producing Escherichia coli. Stores A and B used immersion to marinade raw beef cuts, whereas stores C-1 and C-2 used vacuum tumbling. In general, marinade temperatures at the stores ranged from 1.8 to 6.6°C, and beef cuts were marinated from a few minutes to up to 3 days. Regardless of the process used to marinade meat, levels of M-APC and P-APC in fresh marinades ranged from 3.4 to 4.7 and 1.4 to 1.8 log CFU/mL, respectively, whereas Enterobacteriaceae were not detected in any fresh marinades, even after enrichment. However, levels of M-APC, P-APC, and Enterobacteriaceae in spent marinades collected from stores C-1 and C-2 (ca. 3.6 to 7.1 log CFU/mL) were significantly higher (P &lt; 0.05) compared with levels of these same types of bacteria enumerated from spent marinades collected at stores A and B (ca. ≤0.7 to 4.9 log CFU/mL). None of the 115 marinade samples tested positive for Shiga toxin–producing E. coli by using a BAX system real-time PCR assay. No significant (P &gt; 0.05) association was observed between microbial levels (i.e., M-APC, P-APC, and Enterobacteriaceae) and the temperature or duration of the marination process. Levels of M-APC, P-APC, and Enterobacteriaceae in spent marinades were significantly affected by the marination method (P &lt; 0.05), with levels, in general, being higher in marinades used for tumbling. Thus, retailers must continue to keep marinade solutions and meat at a safe temperature (i.e., ≤4°C) and to properly and frequently sanitize the equipment and environment in both the processing area and deli case.

Bacterial Contamination of Ready-to-Eat Foods and Fresh Products in Retail Shops and Food Factories

1999 ◽  
Vol 62 (6) ◽  
pp. 644-649 ◽  
Author(s):  
KEN-ICHI KANEKO ◽  
HIDEKI HAYASHIDANI ◽  
YOSHIMITSU OHTOMO ◽  
JUNKO KOSUGE ◽  
MASAHIKO KATO ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacterial Contamination ◽  
Plate Count ◽  
E Coli ◽  
Cooked Rice ◽  
Two Samples ◽  
Cooked Food ◽  
Aerobic Plate Count ◽  
Fresh Products

Raw vegetables cut for salad, cooked salad, cooked rice, boiled noodles, bean curd, and cooked Japanese foods were purchased in 27 retail shops in Tokyo. Intact vegetables before being processed and ready-to-eat fresh salad products were obtained from two food factories located in the suburbs of Tokyo. Two hundred thirty-eight retail samples, 137 samples of intact vegetables, and 159 samples of fresh products were examined for aerobic plate count (APC), coliforms, Escherichia coli, Listeria spp., Staphylococcus aureus, and Bacillus cereus. The APC of retail foods were 2.1 to 5.7 log CFU/g, and the range for the coliforms was 0.1 to 2.3 log CFU/g. The APC and coliform values showed that the raw vegetables cut for salad were the most heavily contaminated among the six kinds of ready-to-eat foods examined. Although L. monocytogenes was not detected, two samples of raw vegetables and five kinds of cooked foods yielded Listeria spp. S. aureus was detected in one sample of Japanese cooked food. The APC of the intact vegetables were 2.9 to 7.3 log CFU/g upon arrival and 2.2 to 7.2 log CFU/g after 3 days storage at 10°C. The APC of the fresh products were 3.4 to 7.6 log CFU/g upon arrival and 4.7 to 8.7 log CFU/g after 3 days storage at 10°C. The isolation rates for coliforms were 6.1 to 50% for intact vegetables and 50 to 66.7% for fresh products. E. coli was detected only in the fresh products. B. cereus was isolated from 20.1% (17 of 81) of the intact vegetables and 9.2% (8 of 87) of the fresh products.

Microbiological Load and Zoonotic Agents in Beef Mortadella from Addis Ababa City Supermarkets

2015 ◽  
Vol 78 (5) ◽  
pp. 1043-1045 ◽  
Author(s):  
ADEM HIKO ◽  
GOBENA AMENI ◽  
NINA LANGKABEL ◽  
REINHARD FRIES
Keyword(s):  
Escherichia Coli ◽  
Shelf Life ◽  
Microbial Contamination ◽  
Addis Ababa ◽  
Plate Count ◽  
E Coli ◽  
Aerobic Plate Count

Processing changes the meat ecosystem and, hence, has an impact on the microbiological load and quality of the product. Microbial contamination decreases shelf life and, if a pathogen is present, increases the risk of zoonoses in humans. The aim of this study was to assess the microbiological load, including zoonotic agents, in ready-to-eat beef mortadella from Ethiopian supermarkets. A total of 119 samples from 8 supermarkets in Addis Ababa City (Ethiopia) were examined for aerobic plate count, Enterobacteriaceae, Salmonella, and Escherichia coli. An overall mean of 4.0 log for aerobic plate count and 2.2 log for Enterobacteriaceae was observed. The Salmonella prevalence was 0.8%. An overall E. coli prevalence of 29% (range, 13 to 53%) was observed at individual supermarkets.

Microbiology of the Swine Head Meat Deboning Process

1998 ◽  
Vol 61 (2) ◽  
pp. 249-252 ◽  
Author(s):  
CHERYL LAUBACH ◽  
JEFFREY RATHGEBER ◽  
ALAN OSER ◽  
SAMUEL PALUMBO
Keyword(s):  
Escherichia Coli ◽  
Oral Cavity ◽  
Dna Hybridization ◽  
Plate Count ◽  
Salmonella Spp ◽  
E Coli ◽  
Aerobic Plate Count

The microbiology of swine head meat was evaluated by comparing the levels of aerobic plate count (APC), coliforms, and Escherichia coli as well as the incidence and levels of Salmonella spp. in swine head meat harvested by either the old or a new improved procedure. Based on 144 samples (72 by each procedure), the levels of APC, coliforms, and E. coli were 4.52 ± 0.26, 2.37 ± 0.42, and 2.25 ± 0.42 log10 CFU/g respectively, regardless of the procedure used for harvesting the meat. The incidence (27/72 versus 28/72) and MPN levels (4 to 93 versus 4 to 1100) of Salmonella spp. determined by the Bacteriological Analytical Manual (BAM) methods also were the same for meat obtained using either the old or the new procedure. The BAM method detected a higher incidence of Salmonella spp. (55/144) than other methods, PCR (38/144; BAX, Du Pont) or DNA hybridization (41/144; Gene-Trak). Time of harvesting during the processing day or site of origin of the head meat (cheek versus tongue versus back of head) had no effect on the incidence of Salmonella spp. The data in this study indicate that the levels of bacteria encountered in swine head meat are a reflection of the harvesting procedures themselves, as well as levels in and around the head and oral cavity of swine.

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