scholarly journals Survey of Mycotoxigenic Fungi in Concentrated Poultry Feed in Niger State, Nigeria

2013 ◽  
Vol 2 (2) ◽  
pp. 128 ◽  
Author(s):  
Adeniran Lateef Ariyo ◽  
Makun Hussaini Anthony ◽  
Muhammad Hadiza Lami
Keyword(s):  
Aspergillus Flavus ◽  
Animal Production ◽  
Poultry Feed ◽  
Poultry Feeds ◽  
Commercial Feed ◽  
Mycotoxigenic Fungi ◽  
Vital Component ◽  
Penicillium Spp ◽  
Feed Samples

In Nigeria, concentrated poultry feed is a vital component of animal production and health but there was no reliable mycogram in Niger state, Nigeria. As a result this study determined the incidence of mycotoxigenic fungi in both commercial and privately milled concentrated poultry feeds. A total of 100 poultry feed samples consisting of 52 privately milled poultry feed and 48 commercial feed samples were collected. Mycoflora in the feed was determined. Nine fungi genera were isolated. The most frequently isolated fungi genera in both privately milled and commercial feed was <em>Aspergillus</em> <em>spp </em>which was about 40% of mould isolate. <em>Penicillium spp </em>is 20% in private feed and 13% in commercial feed. A total of 874 fungi were isolated consisting of 458 fungi species in privately milled feed and 416 fungi species found in commercial feed. Mycotoxigenic fungi genera, <em>Aspergillus, Penicillium, Fusarium and Alternaria</em> were isolated. <em>Aspergillus flavus</em> is the commonest isolated fungi species.

scholarly journals An investigation into the microbial infestation of poultry feeds

2016 ◽  
Vol 13 (1) ◽  
pp. 79-86
Author(s):  
MM Rahman ◽  
MS Islam ◽  
MZ Alam ◽  
M Ashrafuzzaman
Keyword(s):  
Aspergillus Niger ◽  
Moisture Content ◽  
Aspergillus Flavus ◽  
Fungal Species ◽  
Fusarium Spp ◽  
Fungal Population ◽  
Fungal Flora ◽  
Poultry Feeds ◽  
Penicillium Spp ◽  
Feed Samples

The Experiments were carried out during the period of July 2001 to December 2002. Infestation of poultry feeds by fungi and aflatoxins content in feeds were determined. Ten feed samples were collected from ten different farms located in three different areas of Bangladesh. Five different fungal species belonging four genera were detected which were Aspergillus flavus, Aspergillus niger, Fusarium spp, Rhizopus stolonifer and Penicillium spp. Among them Aspergillus flavus and Aspergillus niger were found in all the feed samples and the rest were occasionally found. Effect of storage and moisture content of feeds were studied for determining the influence on occurrence of fungal flora in feeds. Higher the storing period of feed along with higher moisture content resulted superior incidences of fungal population in stored feeds. Studied on storage practice with 4 duration of F1 sample revealed that the presence of aflatoxins were 30.52ppb, 33.36ppb, 37.32ppb and 48.35ppb of 5, 10, 15 and 20 days after production of feeds, respectively.J. Bangladesh Agril. Univ. 13(1): 79-86, June 2015

scholarly journals FUNGAL COMPOSITION AND PROXIMATE ANALYSIS OF POULTRY FEEDS SOLD IN BENIN CITY, NIGERIA

2021 ◽  
Vol 2 (2) ◽  
pp. 109-115
Author(s):  
A. S. Ogbebor ◽  
A. A. Imoni ◽  
O. R. Ohiorenoya
Keyword(s):  
Aspergillus Flavus ◽  
Morphological Characteristics ◽  
Proximate Analysis ◽  
Fungal Species ◽  
Control Measures ◽  
Poultry Feed ◽  
Trichoderma Spp ◽  
Poultry Feeds ◽  
Benin City ◽  
Penicillium Spp

Poultry farming contributes significantly to poverty alleviation by providing employment opportunities to Nigerians. Feed quality is the most prominent challenge of the poultry sector. This study was aimed at assessing the fungal and proximate qualities of poultry feeds sold in Benin City, Nigeria. Poultry feed samples (starter, grower and finisher) were obtained from five stores in Benin City. Enumeration and isolation of fungi was carried out using the spread plate method on potato dextrose agar and Aspergillus flavus and parasiticus agar (AFPA). Identification of fungal isolates was based on cultural and morphological characteristics.  Proximate composition was determined by standard methods. The total fungal counts of starter, grower and finisher feeds ranged from 0.10 – 8.50 x 105 sfu/g. Fungal species isolated were Aspergillus flavus, Aspergillus niger, Penicillium spp., Saccharomyces spp., Trichoderma spp., Rhizopus spp. and Mucor spp. The results from proximate analysis were as follows: 36.50±0.92 - 70.92±0.51% (protein), 3.53±0.04 - 8.45±0.16% (fibre), 11.61±0.72 - 13.91±0.27% (fat), 2.70 - 8.60% (moisture content) and 3.65 - 37.83% (carbohydrate). The temperature and relative humidity of storage ranged from 33.90oC - 35.20oC and 69% - 75% respectively. Results showed that poultry feeds sampled have diverse fungi present in them. Control measures should be adhered to in poultry industries and stores to prevent microbial contamination.

scholarly journals Evaluation of microbial quality and pathogenic potentiality of enterobacteria in poultry feeds

2019 ◽  
Vol 28 ◽  
pp. 59-68
Author(s):  
S Islam ◽  
N Tanjila ◽  
MF Begum
Keyword(s):  
Escherichia Coli ◽  
Morphological Characteristics ◽  
Coliform Bacteria ◽  
Poultry Feed ◽  
Plate Count ◽  
Test Results ◽  
Poultry Feeds ◽  
E Coli ◽  
Aerobic Plate Count ◽  
Feed Samples

The present investigation has been carried out to assess the microbial safety and pathogenic potentialities of enterobacteria in poultry feeds. From the results it was observed that total aerobic plate count of poultry feeds samples were recorded as 2.8 × 105 to 5.8 × 109 cfu/g and 100% samples contained ≥106 cfu/g while the highest mean of cfu was counted as log10 8.797/gm. Large number of coliforms were recorded in different poultry feed samples and the ranges of cfu were counted as 1.2 × 104 to 5.2 × 107/g while average 75% samples were contaminated with coliform bacteria with ≥104 cfu/g and the highest mean of cfu was counted as log10 6.103/g. The ranges of cfu of Escherichia coli were 1.03 × 102 to 1.09 × 105/g and 70% samples contained ≥102 cfu/g while the highest mean of cfu was counted as log10 4.493/gm. But the ranges of cfu of total Salmonella sp. were recorded as 1.02 × 101 to 5.25 × 104/g and 50% samples contained ≥102 cfu/g and the highest mean of cfu was counted as log10 3.665/g. Total 29 enterobacterial isolates were isolated from the feed by using selected media. On the basis of morphological characteristics and biochemical test results the isolates were identified as Salmonella sp., Shigella sp., Klebsiella sp., Citrobacter sp., Proteus sp., Enterobacter sp. and Escherichia coli. These isolates were tested on blood agar medium and only seven isolates showed positive β-hemolytic activity.In virulence efficacy test, only hemolytic positive isolates were ingested to chicken and observed that E. coli (SGE-1), Klebsiella sp. (SSE-6) and Salmonella sp. (JSS-9) isolates were highly toxic because the experimental chickens were died after 3 days of ingestion of the bacteria, two isolates showed loose motion symptom after 15 days while other isolates showed little sickness. All the selected isolates showed positive hem-agglutination reactivity in poultry RBC. The results indicate that the poultry feeds were highly contaminated with pathogenic enterobacteria which are risk to public health. J. bio-sci. 28: 59-68, 2020

scholarly journals The presence of potentially toxigenic fungi in poultry feed

2008 ◽  
Vol 24 (5-6) ◽  
pp. 87-93 ◽  
Author(s):  
Vesna Krnjaja ◽  
Lj. Stojanovic ◽  
R. Cmiljanic ◽  
S. Trenkovski ◽  
D. Tomasevic
Keyword(s):  
Animal Husbandry ◽  
Poultry Feed ◽  
Fungal Contamination ◽  
Poultry Feeds ◽  
Toxigenic Fungi ◽  
Total Fungi ◽  
Feed Samples

In Serbia, commercial feedstuffs are an important component in modern animal husbandry, but there is no information available about fungal contamination. Because of that the aim of this study was to determine the mycoflora incidence in poultry feeds. A total of 230 samples of poultry feeds were examined for total fungi count and the presence of potential toxigenic fungi genera. Total fungi count were 1-9 x 104 CFU g-1 in the most of investigated poultry feed samples (38.26%). The most prevalent fungi genera were Fusarium (56.09%) and Aspergillus (54.35%), followed by Rhizopus (40%), Penicillium (30.87%), Mucor (30.04%) and the least frequency species were from genus Alternaria (3.48%).

Burden of Aflatoxin in Poultry Feeds in Selected Chicken Rearing Villages of Bishoftu-ethiopia

2020 ◽  
Author(s):  
Yoseph Cherinet Megerssa ◽  
Tadesse Sisay Kassaw ◽  
Fanos Tadesse Woldemariyam
Keyword(s):  
Human Health ◽  
Aflatoxin Contamination ◽  
Poultry Feed ◽  
Preventive Strategies ◽  
Poultry Industry ◽  
Sectional Study ◽  
Cattle Feed ◽  
Poultry Feeds ◽  
The Mean ◽  
Feed Samples

Abstract Background: Aflatoxins are major contaminants of feed used in poultry industry that negatively affect animal and human health. In Ethiopia, previous studies on aflatoxins mainly considered cattle feed and milk, but scarce information exists for poultry feeds.Method: The aim of this study was to determine the burden of aflatoxin in poultry feed in bishoftu.Cross sectional study was conducted from December, 2018 to May, 2019and 33 compound poultry feed samples were randomly collected from chicken rearing villages of Bishoftuand analyzed for G2, G1, B2 , B1 and total aflatoxins using HPLC.Results: The result indicated thatfrom a total of 33 samples 31(94%) samples were contaminated with aflatoxin. The mean level of aflatoxin G2, G1, B2, B1 and total aflatoxinswere 18.00 µg/g, 88.5499 µg/g, 13.50µg/g, 70.11µg/g and 190.18µg/g respectively. This study curtained the level of aflatoxinin 25 (72.75%) samples for AFT and 22 (66.67%) samples for AFB1 were above the limit of FDA regulatory levels of 20µg/g for poultry feed. Conclusion: The study showed the high contamination of aflatoxins in poultry feed. The study warrants the need for preventive strategies of aflatoxin contamination including implementation of regulatory legislation in poultry feeds in Bishoftu.

scholarly journals Detection of Mycotoxigenic Fungi and Mycotoxins in Poultry Feed of Balochistan Pakistan

2021 ◽  
Vol 72 (1) ◽  
pp. 2741
Author(s):  
FA MAJEED ◽  
A AKBAR ◽  
M SHAFEE ◽  
H SADIA ◽  
S IQBAL ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Aflatoxin Production ◽  
Poultry Feed ◽  
Direct Examination ◽  
Toxic Metabolites ◽  
Mycotoxigenic Fungi ◽  
Poultry Farmers ◽  
Layer Chromatography ◽  
Feed Samples

Mycotoxins are secondary toxic metabolites of fungi, commonly associated to agriculture commodities. Aspergillus, Fusarium, Penicillium and Claviceps genera are the most common mycotoxigenic fungi and the most important types of mycotoxin are Aflatoxins, Zearalenone, Ochratoxin, Fumonisins and Trichothecenes Mycotoxins can be carcinogenic, cytotoxic, mutagenic, teratogenic, neurotoxic, nephrotoxic, estrogenic and immunosuppressant. This study was conducted to examine the presence of mycotoxigenic fungi and mycotoxins in poultry feed used by the poultry farmers of Balochistan. Samples (n=100) were analyzed and found contaminated with fungi such as Aspergillus, Mucor, Rhizopus and Fusarium genera, amongst them Aspergillus species was observed highly prevalent (89%). Aflatoxin production in culture plates were initially screened with the help of ammonia hydroxide vapor test, and subsequently analyzed with the help of AgraStrip®. The Aflatoxins (77%) and Fumonisins (92%) were found in direct examination of feed samples. Toxins presence was also confirmed with the help of ELISA using AgraQuant® kits. Thin Layer Chromatography (TLC) and High-Performance Liquid Chromatography (HPLC) were used for characterization and quantification of aflatoxins. It was concluded that the feed samples were abundantly contaminated with aflatoxins and fumonisins and the fungi responsible for their production. This contaminated feed is one of the reasons for economic lossesfor poultry farmers of the region.

scholarly journals Avaliação da micoflora e ocorrência de micotoxinas em cascas de amendoim em diferentes estágios de maturação da vagem

2008 ◽  
Vol 32 (5) ◽  
pp. 1380-1386 ◽  
Author(s):  
Edlayne Gonçalez ◽  
Tiago Noel de Souza ◽  
Maria Helena Rossi ◽  
Joana D'arc Felicio ◽  
Benedito Corrêa
Keyword(s):  
Arachis Hypogaea ◽  
Aspergillus Flavus ◽  
Sao Paulo ◽  
São Paulo ◽  
Fusarium Spp ◽  
Arachis Hypogaea L ◽  
Penicillium Spp

As cascas de amendoim (Arachis hypogaea L.) são de grande importância para confecção de cama de frangos, de gado de leite e como fonte de fibras para ruminantes, portanto a elucidação dos mecanismos de contaminação por fungos toxigênicos e por micotoxinas em amendoim é imprescindível, especialmente para que medidas preventivas possam ser tomadas. Realizou-se, este trabalho, em Junqueirópolis, Estado de São Paulo, Brasil. Os principais fungos isolados nas cascas de amendoim foram Fusarium ssp. (78,75 %), Rhizopus ssp. (14,1 %) e A. flavus (11,75 %). No solo foram isolados Penicillium spp., Fusarium spp. e Aspergillus flavus, entre outros. Aflatoxinas foram detectadas em amostras de cascas de amendoim a partir do estágio de granação em concentrações que variaram de 5,42 μg/kg a 218,52 μg/kg. Ácido ciclopiazônico e fumonisinas B1 e B2 não foram detectadas. A presença de A. flavus e aflatoxinas nas amostras, revela a importância de um controle das cascas de amendoim antes de sua utilização. Boas práticas agrícolas são indicadas para região, uma vez que a contaminação das vagens ocorreu antes da colheita.

scholarly journals Antifungal activities of thiosemicarbazones and semicarbazones against mycotoxigenic fungi

2014 ◽  
Vol 38 (6) ◽  
pp. 531-537 ◽  
Author(s):  
Rojane de Oliveira Paiva ◽  
Lucimar Ferreira Kneipp ◽  
Carla Marins Goular ◽  
Mariana Almeida Albuquerque ◽  
Aurea Echevarria
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Flavus ◽  
Fungicidal Activity ◽  
Fusarium Verticillioides ◽  
Animal Health ◽  
Fungistatic Activity ◽  
Mycotoxigenic Fungi ◽  
Quality Of Food ◽  
Chelating Effect

Mycotoxigenic fungi can compromise the quality of food, exposing human and animal health at risk. The antifungal activity of eight thiosemicarbazones (1-8) and nine semicarbazones (9-17) was evaluated against Aspergillus flavus, A. nomius, A. ochraceus, A. parasiticus and Fusarium verticillioides. Thiosemicarbazones had MIC values of 125-500 µg/ml. The thiosemicarbazones 1 and 2 exerted fungistatic activity against Aspergillus spp., and thiosemicarbazone 2 exerted fungicidal activity against F. verticillioides. Compound 2 showed an iron chelating effect of 63%. The ergosterol content of A. parasiticus had a decrease of 28 and 71% for the 31.2 and 62.5 µg/ml concentrations of thiosemicarbazone 2 compared to the control. The obtained results of antifungal activity revealed that thiosemicarbazone class was more active when compared to semicarbazone class and, the thiosemicarbazone 2 was the most active compound, specially, against Aspergillus spp.

Aflatoxin-producing potential of Aspergillus flavus strains isolated from Spanish poultry feeds

1986 ◽  
Vol 95 (3) ◽  
pp. 129-132 ◽  
Author(s):  
Miguel A. Moreno Romo ◽  
Guillermo Su�rez Fern�ndez
Keyword(s):  
Aspergillus Flavus ◽  
Poultry Feeds

scholarly journals Aspergillus flavus Producing Aflatoxins Isolated from Materials of Commercial Feed in Japan

2010 ◽  
Vol 44 (4) ◽  
pp. 421-427
Author(s):  
Ryuichi UEGAKI ◽  
Takao TSUKIBOSHI ◽  
Yimin CAI
Keyword(s):  
Aspergillus Flavus ◽  
Commercial Feed
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