scholarly journals Feeding Strategy of Ruminants and Its Potential Effect on Methane Emission Reduction

2016 ◽  
Vol 8 (9) ◽  
pp. 199
Author(s):  
B. Suwignyo ◽  
B. Suhartanto ◽  
N. Umami ◽  
N. Suseno ◽  
Z. Bachruddin
Keyword(s):  
Methane Production ◽  
Rumen Fluid ◽  
Feeding Strategy ◽  
Live Weight ◽  
Methanogenic Bacteria ◽  
Potential Effect ◽  
Universal Primers ◽  
Fibrobacter Succinogenes ◽  
Global Environmental ◽  
Environmental Contribution

<p>A study was conducted to determine the potential effect of <em>Leucaena</em><em> </em><em>leucocephala</em>in the diet with 3 levels 0%, 6%, 12% of ration on the population of rumen <em>methanogenic</em> bacteria of cattle and buffalo. Three each ruminally-fistulated (body weight 342 ± 66.93 kg) were used in this experiment. The amount of feed offered was 2.5% of live weight on DM basis. Rumen fluid was collected from each animal before feeding, after 17 days on feed. The rumen fluid was strained it through cheesecloth and stored in freezer prior to analysis. The samples were subjected to DNA extraction and amplification. Three universal primers were used to detect methanogenic bacteria, which had more than one band, ranging from 500 bp and 1.4 kbp. The results indicated that the level of <em>Leucaena</em><em> </em><em>leucocephala</em><em> </em>in the diets reduced the population of <em>methanogenic</em> rumen bacteria of the cattle and enhanced the <em>Fibrobacter succinogenes</em>. Thus, reduction of methane production increases rumen propionate since methane production is inversely proportional with propionate production. <em>Leucaena</em><em> </em><em>leucocephala</em> give many benefit e.g. for ruminant that will have a good impact in the term of ruminant nutrition and global environmental contribution through reducing methanogens in the rumen.</p>

Association of methanogenic bacteria with rumen protozoa

1983 ◽  
Vol 29 (6) ◽  
pp. 676-680 ◽  
Author(s):  
Lee R. Krumholz ◽  
Cecil W. Forsberg ◽  
Douglas M. Veira
Keyword(s):  
Fluorescence Microscopy ◽  
Methane Production ◽  
Urease Activity ◽  
Rumen Fluid ◽  
Methanogenic Bacteria ◽  
Methanogenic Activity ◽  
Rumen Protozoa ◽  
Complex Population ◽  
Gravity Sedimentation

Methanogenic bacteria superficially associated with rumen entodiniomorphid protozoa were observed by fluorescence microscopy. A protozoal suspension separated from strained rumen fluid (SRF) by gravity sedimentation exhibited a rate of methane production six times greater (per millilitre) than SRF. The number of protozoa (per millilitre) in the protozoal suspension was three times greater than that of SRF; however, the urease activity of this fraction was half that of SRF. The methanogenic activity of SRF and the discrete fractions obtained by sedimentation of protozoa correlated with the numbers of protozoa per millilitre in each fraction. Gravity-sedimented protozoa, washed four times with cell-free rumen fluid, retained 67–71% of the recoverable methanogenic activity. Thus it is evident from our observations that many methanogens adhere to protozoa and that the protozoa support methanogenic activity of the attached methanogens. When protozoa-free sheep were inoculated with rumen contents containing a complex population of protozoa, methanogenic activity of the microflora in SRF samples was not significantly enhanced.

scholarly journals Reducing enteric methane production from buffalo (Bubalus bubalis) by garlic oil supplementation in in vitro rumen fermentation system

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Avijit Dey ◽  
Shyam Sundar Paul ◽  
Puran Chand Lailer ◽  
Satbir Singh Dahiya
Keyword(s):  
Environmental Pollution ◽  
Methane Production ◽  
Rumen Fluid ◽  
Bubalus Bubalis ◽  
Rumen Fermentation ◽  
Acetyl Esterase ◽  
Garlic Oil ◽  
Enteric Methane ◽  
In Vitro Rumen Fermentation

AbstractEnteric methane production contributes significantly to the greenhouse gas emission globally. Although, buffaloes are integral part of livestock production in Asian countries, contributing milk, meat and draft power, the contribution of enteric methane to environmental pollution attracts attention. The present study investigated the efficacy of garlic (Allium sativum) oil in reducing enteric methane production from buffaloes (Bubalus bubalis) by in vitro rumen fermentation. Garlic oil (GOL) was tested at four concentrations [0 (Control), 33.33 µl (GOL-1), 83.33 µl (GOL-2) and 166.66 µl (GOL-3) per litre of buffered rumen fluid] in 100-ml graduated glass syringes and incubated at 39℃ for 24 h for in vitro rumen fermentation study. Supplementation of GOL-1 increased (p < 0.05) total gas production in comparison with GOL-3; however, it remained comparable (p > 0.05) with control and GOL-2. Graded doses of garlic oil inclusions reduced (p < 0.001) methane concentration (%) in total gas and total methane production (ml/g DM), irrespective of concentrations. The feed degradability, volatile fatty acids and microbial biomass production (MBP) were not affected (p > 0.05) by GOL-1, but these tended to decrease in GOL-2 with marked reduction (p < 0.01) in GOL-3. The decrease (p < 0.01) in NH3–N concentration in fermentation fluid in the presence of garlic oil, irrespective of concentration, suggests reduced deamination by inhibiting rumen proteolytic bacterial population. The activities of ruminal fibrolytic enzymes (CMCase, xylanase, β-glucosidase, acetyl esterase) were not affected by lower dose (GOL-1) of garlic oil; however, reduction (p < 0.05) of these enzymes activity in rumen liquor was evident at higher doses (GOL-2 and GOL-3) of supplementation. This study shows positive impact of garlic oil supplementation at low dose (33.33 µl/l of rumen fluid) in reducing enteric methane production, thereby, abatement of environmental pollution without affecting feed digestibility.

Effect of offering three amounts of maize stover to dairy cows on intake and selection

1997 ◽  
Vol 1997 ◽  
pp. 85-85
Author(s):  
J. N. Methu ◽  
E. Owen ◽  
A. Abate ◽  
M. Scarr ◽  
J. Tanner
Keyword(s):  
Dairy Cows ◽  
Crude Protein ◽  
Feeding Strategy ◽  
Latin Square ◽  
Live Weight ◽  
Leaf Sheath ◽  
Barley Straw ◽  
Maize Stover ◽  
Ad Libitum ◽  
Friesian Cows

Several studies with barley straw (e.g. Wahed et al, 1990) and sorghum stover (e.g. Osafo, 1993) have shown improvements in intake with increasing ‘ad libitum’ amounts offered. Furthermore, these studies have demonstrated that sheep and goats offered barley straw or sorghum stover in long, unprocessed form, increase intake by selecting for leaf and leaf sheath, and against stem. This phenomenon offers a simple feeding strategy to use selective feeding behaviour to improve intake and hence production. The aim of the present experiment was to study the effect of offering increasing amounts of long maize stover on intake and selection by dairy cows.Six, early- to mid-lactation Ayrshire and Friesian cows (live weight, M, 430 kg) were used in a double 3x3 Latin square design with 24-day (d) feeding periods. Cows were offered long (unchopped) maize stover at low, medium or high ‘ad libitum’ rates, i.e. 31, 59 or 87 g dry matter (DM)/kg M.d. Cows also received 3.2 kg DM/d of cotton seed cake (339 g/kg DM crude protein) in two meals at milking (0700 h and 1800 h). Stover was offered in one meal daily, at 0700 h, after collecting refusals from the previous day. Amounts of stover offered and refused were weighed daily. Samples of offered stover (0.5 kg) and refused stover (0.5 kg) were analysed for DM daily. All refused stover and 4.0 kg samples of offered stover were botanically fractionated, daily, into stem (S), leaf (L), sheath (Sh) and husk (H). Milk yield was recorded daily and cows weighed at the start and end of each period.

scholarly journals Non-structural carbohydrates in the nutrition of high-yielding dairy cows during a transition period

2010 ◽  
Vol 55 (No. 11) ◽  
pp. 468-478 ◽  
Author(s):  
K. Poláková ◽  
V. Kudrna ◽  
A. Kodeš ◽  
B. Hučko ◽  
Z. Mudřík
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Body Condition ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Live Weight ◽  
Dry Matter Intake ◽  
Control Group ◽  
Maize Starch ◽  
Milk Performance

The main aim of this study was to investigate experimentally the effect of different composition of non-structural carbohydrates (NFC) in prepartum feed rations administered to high-yielding dairy cows at a high concentration of NFC in the diet on dry matter intake both before and after parturition and on subsequent milk performance, body condition and physiological traits of rumen fluid and blood. Thirty-six high-yielding dairy cows were allocated into one of the three well-balanced groups (K, O, and C), and each group received a different feeding rations. Feeding rations differed in non-structural carbohydrate (NFC) structure. The "K" (control) group received a feeding ration with NFC in the form of maize starch in particular, while the feeding rations of the other two (experimental) groups contained either (besides maize starch) saccharose from dried sugar beet (the "O" group) or a dominant amount of NFC was in the form of saccharose (the "C" group). After calving, all dairy cows were given the same feeding ration from the first day after parturition. The experiment was conducted for 21 days before and 50 days after calving. FR in the form of total mixed ration was offered ad libitum. Dry matter intake, milk performance, body condition, live weight, and blood and rumen parameters were recorded for the duration of the experiment. Average daily dry matter intake before calving was highest in the "K" group (14.32 kg per head). Differences among groups were statistically significant (P &lt; 0.05). Prepartum dry matter consumption dropped as the rate of saccharose in the diet of cows increased. Dry matter consumption levelled off after calving. Milk yield was also highest in the "K" group (43.71 kg/head/day), but fatness of milk and thus the production of fat corrected milk were lowest in this group. The highest milk fat content (4.10%) and fat corrected milk production (44.03 kg/head/day) were recorded in the "C" group, whereas the highest milk protein concentration was found in the milk of the "O" group. The composition of NFC affected dry matter intake before parturition, but these concentrations did not significantly affect dry matter intake, milk yield, milk composition, live weight, body condition or blood serum and rumen fluid parameters after calving

An initial investigation on rumen fermentation pattern and methane emission of sheep offered diets containing urea or nitrate as the nitrogen source

2012 ◽  
Vol 52 (7) ◽  
pp. 653 ◽  
Author(s):  
L. Li ◽  
J. Davis ◽  
J. Nolan ◽  
R. Hegarty
Keyword(s):  
Fatty Acid ◽  
Methane Production ◽  
Volatile Fatty Acid ◽  
Rumen Fluid ◽  
Calcium Nitrate ◽  
Rumen Fermentation ◽  
Molar Ratio ◽  
Volatile Fatty Acid Concentration ◽  
Enteric Methane ◽  
Fermentation Pattern

The effects of dietary nitrate and of urea on rumen fermentation pattern and enteric methane production were investigated using 4-month-old ewe lambs. Ten lambs were allocated into two groups (n = 5) and each group was offered one of two isonitrogenous and isoenergetic diets containing either 1.5% urea (T1) or 3% calcium nitrate (T2). Methane production was estimated using open-circuit respiration chambers after 6 weeks of feeding. No difference in nitrogen (N) balance, apparent digestibility of N or microbial N outflow existed between treatments (P > 0.05). Animals offered the T2 diet lost less energy through methane than did those fed the T1 diet (P < 0.05). Total volatile fatty acid concentration, molar proportion of propionate, and the molar ratio of acetate to propionate in rumen fluid were not affected by dietary N source. Compared with urea inclusion, nitrate inclusion caused a significantly higher acetate and lower butyrate percentage in rumen volatile fatty acid. Nitrate supplementation tended to lower methane production by ~7.7 L/day relative to urea supplementation (P = 0.06). Methane yield (L/kg DM intake) was reduced (P < 0.05) by 35.4% when 1.5% urea was replaced by 3% calcium nitrate in the diet. Emission intensity (L methane/kg liveweight gain) was ~17.3% lower in the nitrate-supplemented sheep when compared with urea-fed sheep; however, the reduction was not statistically significant (P > 0.05). This study confirms that the presence of nitrate in the diet inhibits enteric methane production. As no clinical symptoms of nitrite toxicity were observed and sheep receiving nitrate-supplemented diet had similar growth to those consuming urea-supplemented diet, it is concluded that 3% calcium nitrate can replace 1.5% urea as a means of meeting ruminal N requirements and of reducing enteric methane emissions from sheep, provided animals are acclimated to nitrate gradually.

scholarly journals Genomic predictions for enteric methane production are improved by metabolome and microbiome data in sheep (Ovis aries)

2020 ◽  
Vol 98 (10) ◽  
Author(s):  
Elizabeth M Ross ◽  
Ben J Hayes ◽  
David Tucker ◽  
Jude Bond ◽  
Stuart E Denman ◽  
...  
Keyword(s):  
Methane Production ◽  
Prediction Accuracy ◽  
Live Weight ◽  
Ovis Aries ◽  
Prediction Algorithm ◽  
Genomic Relationship ◽  
Time Points ◽  
Genomic Relationships ◽  
Genomic Predictions ◽  
Time Point

Abstract Methane production from rumen methanogenesis contributes approximately 71% of greenhouse gas emissions from the agricultural sector. This study has performed genomic predictions for methane production from 99 sheep across 3 yr using a residual methane phenotype that is log methane yield corrected for live weight, rumen volume, and feed intake. Using genomic relationships, the prediction accuracies (as determined by the correlation between predicted and observed residual methane production) ranged from 0.058 to 0.220 depending on the time point being predicted. The best linear unbiased prediction algorithm was then applied to relationships between animals that were built on the rumen metabolome and microbiome. Prediction accuracies for the metabolome-based relationships for the two available time points were 0.254 and 0.132; the prediction accuracy for the first microbiome time point was 0.142. The second microbiome time point could not successfully predict residual methane production. When the metabolomic relationships were added to the genomic relationships, the accuracy of predictions increased to 0.274 (from 0.201 when only the genomic relationship was used) and 0.158 (from 0.081 when only the genomic relationship was used) for the two time points, respectively. When the microbiome relationships from the first time point were added to the genomic relationships, the maximum prediction accuracy increased to 0.247 (from 0.216 when only the genomic relationship was used), which was achieved by giving the genomic relationships 10 times more weighting than the microbiome relationships. These accuracies were higher than the genomic, metabolomic, and microbiome relationship matrixes achieved alone when identical sets of animals were used.

Effect of diets differing in the ratio of nolassed sugar beet feed to barley and yeast culture on growth and rumen metabolism of sheep

1993 ◽  
Vol 1993 ◽  
pp. 179-179
Author(s):  
Y. Rouzbehan ◽  
J.A. Rooke ◽  
H. Galbraith ◽  
J.G. Perrott
Keyword(s):  
Weight Gain ◽  
Sugar Beet ◽  
Physical Properties ◽  
Rumen Fluid ◽  
Live Weight ◽  
Partial Substitution ◽  
Yeast Culture ◽  
Growth Responses ◽  
Rumen Metabolism ◽  
Metabolisable Energy

The efficiency of conversion of Metabolisable Energy into live weight gain by growing sheep may be improved by the partial substitution of fibrous diets with barley. This effect is associated with a reduction in the ratio of acetate to propionate in rumen fluid. Previous studies (Galbraith et al. 1988, 1989) have investigated responses of lambs to combinations of molassed sugar beet feed (MSBF) and barley and found a ratio of 0·75:0·25 MSBF:barley to be as effective as 0·25:0·75 when the diets were ground and pelleted.The incorporation of the yeast culture product, Yea-sacc, has been associated with increases in the concentrations of propionate in the rumen and variable growth responses in ruminants. The objectives of the present study were to investigate diets for finishing lambs which differed in the ratios of pelleted shreds of MSBF and barley and the effects of supplementation with Yea-sacc on growth, rumen metabolites and the physical properties of the diets.

scholarly journals In vitro fermentation, digestibility and methane production of tropical perennial grass species

2014 ◽  
Vol 65 (5) ◽  
pp. 479 ◽  
Author(s):  
Belete Shenkute Gemeda ◽  
Abubeker Hassen
Keyword(s):  
Organic Matter ◽  
Methane Production ◽  
Rumen Fluid ◽  
Perennial Grass ◽  
Gas Production ◽  
Automated System ◽  
Grass Species ◽  
Tropical Conditions ◽  
Acid Detergent Fibre

This study characterised 16 tropical perennial grass species in terms of in vitro methane output and related their digestibility and rumen fermentation with methane output. The grass samples were collected, dried in a forced oven, and ground and analysed for nutrient composition. In vitro gas production and organic matter digestibility (IVOMD) were determined using rumen fluid collected, strained and anaerobically prepared. A semi-automated system was used to measure gas production through in vitro incubation at 39°C. Anthephora argentea and Stipagrostis ciliate produced the highest concentration of methane in terms of g kg–1 digestible dry matter (DDM) and g kg–1 digestible organic matter (IVOMD). Cenchrus ciliaris, Setaria verticillata and Panicum coloratum produced the lowest (P < 0.05) methane when expressed in terms of g kg–1 DDM and g kg–1 IVOMD. Ash, ether extract, non-fibrous carbohydrate, neutral and acid detergent insoluble nitrogen, and crude protein were negatively correlated with methane production. Methane production positively correlated with neutral and acid detergent fibre, cellulose and hemicellulose. It is important to focus on screening and selecting perennial grass with higher nitrogen content and low methane production to mitigate methane production under tropical conditions.

scholarly journals Effects of Geraniol and Camphene on in Vitro Rumen Fermentation and Methane Production

2017 ◽  
Vol 48 (2) ◽  
pp. 63-69
Author(s):  
M. Joch ◽  
V. Kudrna ◽  
B. Hučko
Keyword(s):  
Methane Emission ◽  
Methane Production ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Positive Control ◽  
Rumen Fermentation ◽  
Dry Matter Digestibility

AbstractThe objective of this study was to determine the effects of geraniol and camphene at three dosages (300, 600, and 900 mg l-1) on rumen microbial fermentation and methane emission in in vitro batch culture of rumen fluid supplied with a 60 : 40 forage : concentrate substrate (16.2% crude protein, 33.1% neutral detergent fibre). The ionophore antibiotic monensin (8 mg/l) was used as positive control. Compared to control, geraniol significantly (P < 0.05) reduced methane production with increasing doses, with reductions by 10.2, 66.9, and 97.9%. However, total volatile fatty acids (VFA) production and in vitro dry matter digestibility were also reduced (P < 0.05) by all doses of geraniol. Camphene demonstrated weak and unpromising effects on rumen fermentation. Camphene did not decrease (P > 0.05) methane production and slightly decreased (P < 0.05) VFA production. Due to the strong antimethanogenic effect of geraniol a careful selection of dose and combination with other antimethanogenic compounds may be effective in mitigating methane emission from ruminants. However, if a reduction in total VFA production and dry matter digestibility persisted in vivo, geraniol would have a negative effect on animal productivity.

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