Association of methanogenic bacteria with rumen protozoa

1983 ◽  
Vol 29 (6) ◽  
pp. 676-680 ◽  
Author(s):  
Lee R. Krumholz ◽  
Cecil W. Forsberg ◽  
Douglas M. Veira
Keyword(s):  
Fluorescence Microscopy ◽  
Methane Production ◽  
Urease Activity ◽  
Rumen Fluid ◽  
Methanogenic Bacteria ◽  
Methanogenic Activity ◽  
Rumen Protozoa ◽  
Complex Population ◽  
Gravity Sedimentation

Methanogenic bacteria superficially associated with rumen entodiniomorphid protozoa were observed by fluorescence microscopy. A protozoal suspension separated from strained rumen fluid (SRF) by gravity sedimentation exhibited a rate of methane production six times greater (per millilitre) than SRF. The number of protozoa (per millilitre) in the protozoal suspension was three times greater than that of SRF; however, the urease activity of this fraction was half that of SRF. The methanogenic activity of SRF and the discrete fractions obtained by sedimentation of protozoa correlated with the numbers of protozoa per millilitre in each fraction. Gravity-sedimented protozoa, washed four times with cell-free rumen fluid, retained 67–71% of the recoverable methanogenic activity. Thus it is evident from our observations that many methanogens adhere to protozoa and that the protozoa support methanogenic activity of the attached methanogens. When protozoa-free sheep were inoculated with rumen contents containing a complex population of protozoa, methanogenic activity of the microflora in SRF samples was not significantly enhanced.

scholarly journals Feeding Strategy of Ruminants and Its Potential Effect on Methane Emission Reduction

2016 ◽  
Vol 8 (9) ◽  
pp. 199
Author(s):  
B. Suwignyo ◽  
B. Suhartanto ◽  
N. Umami ◽  
N. Suseno ◽  
Z. Bachruddin
Keyword(s):  
Methane Production ◽  
Rumen Fluid ◽  
Feeding Strategy ◽  
Live Weight ◽  
Methanogenic Bacteria ◽  
Potential Effect ◽  
Universal Primers ◽  
Fibrobacter Succinogenes ◽  
Global Environmental ◽  
Environmental Contribution

<p>A study was conducted to determine the potential effect of <em>Leucaena</em><em> </em><em>leucocephala</em>in the diet with 3 levels 0%, 6%, 12% of ration on the population of rumen <em>methanogenic</em> bacteria of cattle and buffalo. Three each ruminally-fistulated (body weight 342 ± 66.93 kg) were used in this experiment. The amount of feed offered was 2.5% of live weight on DM basis. Rumen fluid was collected from each animal before feeding, after 17 days on feed. The rumen fluid was strained it through cheesecloth and stored in freezer prior to analysis. The samples were subjected to DNA extraction and amplification. Three universal primers were used to detect methanogenic bacteria, which had more than one band, ranging from 500 bp and 1.4 kbp. The results indicated that the level of <em>Leucaena</em><em> </em><em>leucocephala</em><em> </em>in the diets reduced the population of <em>methanogenic</em> rumen bacteria of the cattle and enhanced the <em>Fibrobacter succinogenes</em>. Thus, reduction of methane production increases rumen propionate since methane production is inversely proportional with propionate production. <em>Leucaena</em><em> </em><em>leucocephala</em> give many benefit e.g. for ruminant that will have a good impact in the term of ruminant nutrition and global environmental contribution through reducing methanogens in the rumen.</p>

The bacterial numeration and an observation of a new syntrophic association for granular sludge

1997 ◽  
Vol 36 (6-7) ◽  
pp. 133-140 ◽  
Author(s):  
Zhu Jianrong ◽  
Hu Jicui ◽  
Gu Xiasheng
Keyword(s):  
Anaerobic Bacteria ◽  
Granular Sludge ◽  
Methanogenic Bacteria ◽  
Uasb Reactor ◽  
Two Phase ◽  
Methanogenic Activity ◽  
Syntrophic Association ◽  
Group I ◽  
Acetogenic Bacteria ◽  
Fermentative Bacteria

The bacterial numeration and microbial observation were made on granular sludge from laboratory single and two-phase UASB reactors. It was shown that the fermentative bacteria (group I), H2-producing acetogenic bacteria (group II) and methanogenic bacteria (group III) of granular sludge in single UASB reactor were 9.3 × 108−4.3 × 109, 4.3 × 107−4.3 × 108, 2.0−4.3 × 108, respectively, during the granulation process. The sludge of methanogenic reactor exhibited the similar results. That indicates there is no big difference between suspended and granular sludge, and bacterial population for three groups of anaerobic bacteria are similar. The formation of granular sludge depends mainly on the organization and arrangement of bacteria. An observation of granular sludge using electron microscope revealed that the fermentative bacteria and hydrogenotrophic methanogens existed on outer surface of granules, and aceticlastic methanogens and H2-producing acetogenic bacteria occupied the inner layer. A new syntrophic association between Methanosaeta sp. and Syntrophomonas sp. (even plus Methanobrevibacter sp.) was observed. Because Methanosaeta can effectively convert the acetate (the end product of propionate and butyrate) to methane, such a new syntrophic association is supposed to support the degradation of short fatty acids and high methanogenic activity of granular sludge. Based on structural pattern, a hypothesis on mechanism of granulation called “crystallized nuclei formation” is proposed.

scholarly journals Reducing enteric methane production from buffalo (Bubalus bubalis) by garlic oil supplementation in in vitro rumen fermentation system

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Avijit Dey ◽  
Shyam Sundar Paul ◽  
Puran Chand Lailer ◽  
Satbir Singh Dahiya
Keyword(s):  
Environmental Pollution ◽  
Methane Production ◽  
Rumen Fluid ◽  
Bubalus Bubalis ◽  
Rumen Fermentation ◽  
Acetyl Esterase ◽  
Garlic Oil ◽  
Enteric Methane ◽  
In Vitro Rumen Fermentation

AbstractEnteric methane production contributes significantly to the greenhouse gas emission globally. Although, buffaloes are integral part of livestock production in Asian countries, contributing milk, meat and draft power, the contribution of enteric methane to environmental pollution attracts attention. The present study investigated the efficacy of garlic (Allium sativum) oil in reducing enteric methane production from buffaloes (Bubalus bubalis) by in vitro rumen fermentation. Garlic oil (GOL) was tested at four concentrations [0 (Control), 33.33 µl (GOL-1), 83.33 µl (GOL-2) and 166.66 µl (GOL-3) per litre of buffered rumen fluid] in 100-ml graduated glass syringes and incubated at 39℃ for 24 h for in vitro rumen fermentation study. Supplementation of GOL-1 increased (p < 0.05) total gas production in comparison with GOL-3; however, it remained comparable (p > 0.05) with control and GOL-2. Graded doses of garlic oil inclusions reduced (p < 0.001) methane concentration (%) in total gas and total methane production (ml/g DM), irrespective of concentrations. The feed degradability, volatile fatty acids and microbial biomass production (MBP) were not affected (p > 0.05) by GOL-1, but these tended to decrease in GOL-2 with marked reduction (p < 0.01) in GOL-3. The decrease (p < 0.01) in NH3–N concentration in fermentation fluid in the presence of garlic oil, irrespective of concentration, suggests reduced deamination by inhibiting rumen proteolytic bacterial population. The activities of ruminal fibrolytic enzymes (CMCase, xylanase, β-glucosidase, acetyl esterase) were not affected by lower dose (GOL-1) of garlic oil; however, reduction (p < 0.05) of these enzymes activity in rumen liquor was evident at higher doses (GOL-2 and GOL-3) of supplementation. This study shows positive impact of garlic oil supplementation at low dose (33.33 µl/l of rumen fluid) in reducing enteric methane production, thereby, abatement of environmental pollution without affecting feed digestibility.

Modes of transmission of rumen protozoa between mature sheep

2010 ◽  
Vol 50 (6) ◽  
pp. 414 ◽  
Author(s):  
Simon H. Bird ◽  
R. S. Hegarty ◽  
R. Woodgate
Keyword(s):  
Drinking Water ◽  
Rumen Fluid ◽  
Contaminated Water ◽  
Feed Water ◽  
Faecal Material ◽  
Modes Of Transmission ◽  
Rumen Protozoa ◽  
Animal Contact ◽  
Ciliate Protozoa

Three experiments were conducted to evaluate routes by which viable rumen ciliate-protozoa may be transferred between mature sheep. Feed, water and faecal material were tested as possible vectors for protozoal transfer in addition to direct animal to animal contact. In Experiment 1, protozoa-free sheep were either offered or orally dosed with protozoa-contaminated material or allowed contact with faunated animals. The treated sheep were then monitored over a 4-week period for the appearance of protozoa in the rumen. Protozoa were successfully transferred to protozoa-free animals via contaminated water but no transfer occurred via feed or faeces or by direct animal to animal contact. In Experiment 2, the drinking water of penned faunated sheep was found to become contaminated with protozoa within 4–6 h of being placed in the pen. In Experiment 3, nine protozoa-free sheep were grazed in a paddock with a flock of 75 faunated ewes for periods of 1–3 weeks, and protozoa became established in one protozoa-free sheep. The results of these studies suggest that the most likely mode of transfer of protozoal cells from one sheep to another is via water, rather than by rumen fluid contaminating feed, or from faeces of faunated sheep. Further tests are required to demonstrate protozoal transmission via water occur under a range of conditions and inoculum levels.

Sludge accumulation and methanogenic activity in an anaerobic lagoon

2000 ◽  
Vol 42 (10-11) ◽  
pp. 247-255 ◽  
Author(s):  
J. Paing ◽  
B. Picot ◽  
J. P. Sambuco ◽  
A. Rambaud
Keyword(s):  
Anaerobic Digestion ◽  
Methane Production ◽  
Anaerobic Degradation ◽  
Volatile Fatty Acids ◽  
Anaerobic Fermentation ◽  
Methanogenic Activity ◽  
Start Up ◽  
Anaerobic Lagoon ◽  
Methane Production Rate ◽  
Sludge Accumulation

Sludge accumulation and the characteristics of anaerobic digestion in sludge had been investigated in a primary anaerobic lagoon. Methanogenic potential of sludge was evaluated by an anaerobic digestion test which measured the methane production rate. Sludge was sampled at several points in the lagoon to determine spatial variations and with a monthly frequency from the start-up of the lagoon to observe the development of anaerobic degradation. Maximum amounts of sludge accumulated near the inlet. The mean methane production of sludge was 2.9 ml gVS–1 d–1. Sludge near the outlet presented a greater methanogenic activity and a lesser concentration of volatile fatty acids than near the inlet. The different stages of anaerobic degradation were spatially separated, acidogenesis near the inlet and methanogenesis near the outlet. This staged distribution seemed to increase efficiency of anaerobic fermentation compared with septic tanks. Methane release at the surface of the lagoon was estimated to be very heterogeneous with a mean of 25 l m–2 d–1. The development of performance and sludge characteristics showed the rapid beginning of methanogenesis, three months after the start-up of the anaerobic lagoon. Considering the volume of accumulated sludge, it could however be expected that methanogenic activity would further increase.

Analysis of environmental factors affecting methane production from high-solids organic waste

1997 ◽  
Vol 36 (6-7) ◽  
pp. 493-500 ◽  
Author(s):  
J. J. Lay ◽  
Y. Y. Li ◽  
T. Noike ◽  
J. Endo ◽  
S. Ishimoto
Keyword(s):  
Moisture Content ◽  
Methane Production ◽  
Organic Waste ◽  
High Solids ◽  
Methanogenic Activity ◽  
Factors Affecting ◽  
Bacterial System ◽  
Sludge Cake ◽  
Production Curve ◽  
Ph Range

A simple model developed from the Gompertz equation was used to describe the cumulative methane production curve in the batch culture. By using this model, three key parameters, namely methane production rate, potential and lagphase time, in a cumulative methane production curve were exactly estimated based on the experimental data. The results indicate that each gram of dry organic waste of a sludge cake, meat, carrot, rice, potato and cabbage had a methane production potential of 450, 424, 269, 214, 203 and 96 mL, respectively. The methanogenic activity of these digesters decreased with a decrease in the moisture content. The moisture content threshold limit, at which the methanogenic activity dropped to zero, was found to be 56.6% for the sludge cake, but greater than 80% for meat, carrot and cabbage. In the high-solids sludge digestion, the relative methanogenic activity dropped from 100% to 53% when the moisture content decreased from 96% to 90%. The rate of methane production at moisture contents of 90% to 96% functioned in a pH range between 6.6 and 7.8, but optimally at pH 6.8, and the process may fail if the pH was lower than 6.1 or higher than 8.3. On the other hand, the methanogenic activity was dependent on the level of ammonium, NH4+, but not free ammonia, NH3, indicating that the NH4+ was the more significant factor rather than the NH3 in affecting the methanogenic activity of a well-acclimatized bacterial system. In the wide pH range of 6.5 to 8.5, the methanogenic activity decreased with the increase in the NH4+; dropped 10% at the NH4+-N concentration of 1670-3720 mg·L−1, 50% at 4090-5550 mg·L−1 and dropped to zero at 5880-6600 mg·L−1. However, the lagphase time was dependent on the NH3 level, but not on NH4+, and when NH3-N was higher than 500 mg·L−1, a notable shock was observed. This suggests that the NH3 level was the more sensitive factor than the NH4+ level for an unacclimatized bacterial system.

scholarly journals Tailoring Carbon Nanotubes to Enhance their Efficiency as Electron Shuttle on the Biological Removal of Acid Orange 10 Under Anaerobic Conditions

Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2496
Author(s):  
Ana Rita Silva ◽  
O. Salomé G.P. Soares ◽  
M. Fernando R. Pereira ◽  
M. Madalena Alves ◽  
Luciana Pereira
Keyword(s):  
Carbon Nanotubes ◽  
Methane Production ◽  
Carbon Nanomaterials ◽  
Reduction Rate ◽  
Granular Sludge ◽  
Biological Reduction ◽  
Methanogenic Activity ◽  
Acid Orange ◽  
Biological Removal ◽  
Acid Orange 10

Anaerobic treatments have been described for the biodegradation of pollutants. However, the reactions proceed slowly due to the recalcitrant nature of these compounds. Carbon nanomaterials (CNM) intermediate in, and favor, the electron transfer, accelerating the anaerobic reduction of pollutants, which act as final electron acceptors. In the present work, different carbon nanotubes (CNT) with modified surface chemistry, namely CNT oxidized with HNO3 (CNT_HNO3) and CNT doped with nitrogen in a ball milling process (CNT_N_MB) were prepared using commercial CNT as a starting material. The new CNM were tested as redox mediators (RM), 0.1 g L−1, in the biological reduction of the azo dye, Acid Orange 10 (AO10), with an anaerobic granular sludge, over 48 h of reaction. Methane production was also assessed to verify the microorganism’s activity and the CNM’s effect on the methanogenic activity. An improvement in the biological removal of AO10 occurred with all CNM (above 90%), when compared with the control without CNM (only 32.4 ± 0.3%). The best results were obtained with CNT_N_MB, which achieved 98.2 ± 0.1% biological AO10 removal, and an 11-fold reduction rate increase. In order to confer magnetic properties to the CNM, tailored CNT were impregnated with 2% of iron-samples: CNT@2%Fe, CNT@2%Fe_N_MB, and CNT@2%Fe_HNO3. The better performance of the CNT doped with nitrogen was confirmed with CNT@2%Fe_N_MB, and the magnetic character facilitated its recovery after treatment, and did not affect its good catalytic properties. No dye removal was observed in the abiotic assays, so the removal was not due to adsorption on the CNM. Furthermore, the microorganism’s viability was maintained during the assay and methane production was not affected by the presence of the CNM. Despite the toxic character of the aromatic amines formed, detoxification was observed after the biological process with thermally treated CNT.

An initial investigation on rumen fermentation pattern and methane emission of sheep offered diets containing urea or nitrate as the nitrogen source

2012 ◽  
Vol 52 (7) ◽  
pp. 653 ◽  
Author(s):  
L. Li ◽  
J. Davis ◽  
J. Nolan ◽  
R. Hegarty
Keyword(s):  
Fatty Acid ◽  
Methane Production ◽  
Volatile Fatty Acid ◽  
Rumen Fluid ◽  
Calcium Nitrate ◽  
Rumen Fermentation ◽  
Molar Ratio ◽  
Volatile Fatty Acid Concentration ◽  
Enteric Methane ◽  
Fermentation Pattern

The effects of dietary nitrate and of urea on rumen fermentation pattern and enteric methane production were investigated using 4-month-old ewe lambs. Ten lambs were allocated into two groups (n = 5) and each group was offered one of two isonitrogenous and isoenergetic diets containing either 1.5% urea (T1) or 3% calcium nitrate (T2). Methane production was estimated using open-circuit respiration chambers after 6 weeks of feeding. No difference in nitrogen (N) balance, apparent digestibility of N or microbial N outflow existed between treatments (P > 0.05). Animals offered the T2 diet lost less energy through methane than did those fed the T1 diet (P < 0.05). Total volatile fatty acid concentration, molar proportion of propionate, and the molar ratio of acetate to propionate in rumen fluid were not affected by dietary N source. Compared with urea inclusion, nitrate inclusion caused a significantly higher acetate and lower butyrate percentage in rumen volatile fatty acid. Nitrate supplementation tended to lower methane production by ~7.7 L/day relative to urea supplementation (P = 0.06). Methane yield (L/kg DM intake) was reduced (P < 0.05) by 35.4% when 1.5% urea was replaced by 3% calcium nitrate in the diet. Emission intensity (L methane/kg liveweight gain) was ~17.3% lower in the nitrate-supplemented sheep when compared with urea-fed sheep; however, the reduction was not statistically significant (P > 0.05). This study confirms that the presence of nitrate in the diet inhibits enteric methane production. As no clinical symptoms of nitrite toxicity were observed and sheep receiving nitrate-supplemented diet had similar growth to those consuming urea-supplemented diet, it is concluded that 3% calcium nitrate can replace 1.5% urea as a means of meeting ruminal N requirements and of reducing enteric methane emissions from sheep, provided animals are acclimated to nitrate gradually.

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