scholarly journals Respiratory Activity as a Fast Method for Vigor Differentiation of Habanero Pepper Seeds

2019 ◽  
Vol 11 (2) ◽  
pp. 420
Author(s):  
Elise de Matos Pereira ◽  
Aline Silva Freitas ◽  
Heloisa Oliveira dos Santos ◽  
Renato Mendes Guimarães ◽  
Sophia Mangussi Franchi Dutra ◽  
...  

The objective of this study was to evaluate the efficiency and rapidity of the Pettenkofer and Titulation methods in the determination of respiratory activity and its relation with the vigor of habanero pepper seeds. For this, six lots of habanero pepper seeds were evaluated for respiratory activity, through Pettenkofer and Titulation physicochemical methods, and for physiological quality via tests of germination, first germination count, germination speed index, emergence, initial stand, emergence speed index and electrical conductivity. Biochemical analyzes were also carried out using the electrophoresis of the esterase (EC 3.1.1.1.) and alcohol dehydrogenase (EC 1.1.1.2.), and the quantification of the endo-β-mannanase enzyme (EC 3.2.1.78). The tests of physiological quality and the respiratory activity of the seeds allowed the classification of the habanero pepper seed lots at different vigor levels. There was a correlation between the respiratory activity with the tests used to evaluate the physiological quality of the seeds and with the endo-β-mannanase enzyme. It is concluded that the Pettenkofer and Titulation methods are promising for the evaluation of the deterioration level and the discrimination of lots of habanero pepper seeds with different vigor levels.

Author(s):  
Girlânio H. Silva ◽  
Anderson C. José ◽  
Fabrício P. Teixeira ◽  
Luciana M. Gonzaga ◽  
Raul R. Molina ◽  
...  

This work aimed to evaluate the effect of different priming treatments in the longevity of H. serratifolius seeds. Seeds were osmo-conditioned in PEG -1.0 MPa at 10, 15 and 20°C or hydro-primed at 5, 10 and 15°C. Final germination, speed and uniformity of germination were assessed. Priming did not affect the final percentage nor uniformity of germination; however, the germination speed was increased after hydropriming at 15°C and osmo-conditioning at 15°C compared to the control. Primed and not primed seeds were placed into an incubator (25°C, dark, 100% RH) until they reached 15% moisture content. Then, seeds were incubated in a container at 40°C for 0 to 144 hours, so, samples were taken in each period for determination of viability. The results suggest that priming increases longevity of H. serratifolius seeds.


2018 ◽  
Vol 10 (10) ◽  
pp. 343
Author(s):  
Erivanessa C. Sousa ◽  
Moadir S. Leite ◽  
Salvador B. Torres ◽  
Fernando S. Oliveira ◽  
Alek S. Dutra ◽  
...  

Seeds are one of the main inputs used in agriculture, and their quality is directly related to the success of the field crop. Thus, seed companies increasingly need to obtain fast and reliable seed quality results, making it easier to make decisions regarding the use of seed batches. In this sense, the objective was to evaluate the CO2 measurement efficiency in the classification of S. bicolor seed vigor. For this, six batches of cv. Ponta Negra seeds were used, from different national producers. The physiological quality of the seeds was evaluated by means of the following tests: germination, first germination count, germination speed index, accelerated aging, total dry matter of seedlings, electrical conductivity and respiratory activity. The experimental design was completely randomized, with four replications. The Pearson correlation coefficient between the respiratory activity and the germination and vigor tests was determined. The respiratory activity test on S. bicolor seeds was shown to be promising for the identification of differences in vigor between seed batches.


2018 ◽  
Vol 10 (5) ◽  
pp. 312
Author(s):  
Geliandro Anhaia Rigo ◽  
Luis Osmar Braga Schuch ◽  
Willian Silva Barros ◽  
Rodrigo Lamaison de Vargas ◽  
Vinícius Jardel Szareski ◽  
...  

The aim of this work was to correlate macronutrient content of soybean seeds to physiological quality of different cultivars. The work was developed in the Federal University of Pelotas, in the facilities of the Seed Science and Technology Graduate Program. The experimental design was randomized blocks in arranged in four replicates. The following soybean cultivars were used: BMX Apolo RR (12 lots), BMX Ativa RR (13 lots), BMX Energia RR (26 lots), BMX Força RR (24 lots), BMX Impacto RR (35 lots), BMX Magna RR (16 lots), BMX Turbo RR (44 lots), BMX Potência RR (82 lots) and NA 5909 RR (28 lots), with seeds produced in northwest Rio Grande do Sul. The determination of nutritional contents found in plant tissues of soybean seeds, were measured: Nitrogen (N), Phosphorus (P), Potassium (K), Magnesium (Mg), Calcium (Ca), Sulfur (S). The macronutrient contents of the seeds vary according to genetic characteristics of the cultivars, with higher oscillations of nitrogen, phosphorus, magnesium and sulfur contents present in soybean seeds. Potassium and calcium are defined as the most stable nutrients for the cultivars and seed lots analyzed. High concentrations of nitrogen, phosphorus and calcium are determinants for the physiological quality of soybean seeds.


Author(s):  
Marcelo Laranjeira Pimentel ◽  
Henara Valéria Miranda Castro ◽  
Mike Kirixi Munduruku ◽  
Larissa Conceição Cunha Ponte ◽  
Deyvielen Maria Ramos Alves ◽  
...  

The use of fungi in seed treatment can improve the plant's physiological characteristics. The aim of this study was to evaluate the influence of the use of Trichoderma harzianum on the physiological quality of bean seeds. The experiment was set up and conducted in the forest seed laboratory of the Federal University of Western Para, in a randomized design, using 4 procedures on the basis of a colony forming unit (CFU): T1 (0 CFU), T2 (4 x 109 CFU), T3 (8 x 109 CFU) and T4 (12 x 109 CFU), with 5 repetitions. Germination percentage (G%), germination speed index (GSI), hypocotyl length (HL) and radicle length (RL) were evaluated as variables. The data were analyzed by variance analysis and the averages were compared using the Tukey test (p≤0.05), in addition to the regression analysis, using the Minitab© version 18 statistical software. There were differences between the germination percentage tests, in which T2 obtained 100% of germinated seeds, for GSI it was observed that T2 and T3 were equal and superior to the other treatments, while for RL T1 and T2 obtained the best results, however, for HL there were no statistical differences between treatments. T2 was more efficient in the physiological quality of seeds to germination percentage and germination speed index


2016 ◽  
Vol 46 (6) ◽  
pp. 1014-1018 ◽  
Author(s):  
Charline Zaratin Alves ◽  
Lennis Afraire Rodrigues ◽  
Carlos Henrique Queiroz Rego ◽  
Josué Bispo da Silva

ABSTRACT: Crambe is a rapeseed with high oil content and can be used as a winter cover or as a source of raw material for the production of biodiesel, however espite the growing interest in the culture, research on the subject is still incipient, especially concerning the seed production and analysis technology. The purpose of this study is to evaluate the physiological quality of crambe seeds, 'FMS Brilhante' cultivar, by testing the pH of exudate. Five seed lots were submitted to the determination of water content and the tests of germination and vigor (first count, emergence and tetrazolium). In the conduction of pH exudate test, temperatures (25 and 30oC), and periods of seed imbibition in water (15, 30 and 45 minutes) were tested. The experiment was conducted in a completely randomized manner, with four replicates, and the mean values were compared by the Tukey test at 5% probability; Pearson correlation between the pH of the exudate and initial tests was also made. Testing the pH of exudate is promising for separating lots of crambe seeds and the following combinations of 25°C/30 minutes or 30°C/45 minutes can be used.


2005 ◽  
Vol 62 (4) ◽  
pp. 319-324 ◽  
Author(s):  
Mário Lúcio de Carvalho Bittencourt ◽  
Denise Cunha Fernandes dos Santos Dias ◽  
Luiz Antônio dos Santos Dias ◽  
Eduardo Fontes Araújo

The osmotic conditioning or priming is considered a promising technique to speed up germination and to improve seed performance. Four seed lots of asparagus, Mary Washington cultivar, were primed at 25°C for seven or 14 days using PEG 6000 at -1.0 or -1.2 MPa, or sea water at -3.3 MPa; or for three days in distilled water. The physiological quality of the seeds was evaluated by standard germination, first count germination, speed of seedling emergence, and germination percentage and seedling fresh and dry weights after controlled deterioration test. Primed seeds presented higher germination speed, independently of their initial physiological quality. Beneficial effects of priming on germination and vigour were more expressive in the seed lot of low physiological quality. Priming in PEG 6000 at -1.0 MPa for 14 days was the most beneficial treatment to improve asparagus seeds performance.


2016 ◽  
Vol 11 (12) ◽  
pp. 1102-1109 ◽  
Author(s):  
Oliveira dos Santos Heloisa ◽  
Mangussi Franchi Dutra Sophia ◽  
Walace Pereira Rucyan ◽  
Maria De Oliveira Pires Raquel ◽  
Vilela De Resende Von Pinho Eacute dila ◽  
...  

1959 ◽  
Vol 32 (3) ◽  
pp. 898-906
Author(s):  
R. V. Uzina ◽  
L. S. Gromova ◽  
S. A. Vasil'eva

Abstract In selecting methods for determination of rubber to cord bond strength it is necessary to consider the factors which are subject to variation in the system— the type of cord, the composition of the vulcanized rubber, or the composition of the impregnant. The selection (composition) of the methods of assessment of bond strength was carried out in the present study while keeping the type of cord and the rubber compositions constant ; only the composition of the impregnant was varied. Up to the present there has been no single laboratory method for evaluating rubber to cord bond strength for the determination of the service quality of rubber-fabric structures. The bond strength in such a system is assessed in the majority of cases by the use of a series of methods. The existing methods may be systematized according to the nature of the deformation (static or dynamic), the nature of the specimen (with single thread of cord or with cord fabric) and so on. We adopted the following classification of methods: 1). Determination of bond strength of a single thread of cord with the rubber; 2). Determination of bond strength of rubber to fabric model specimens. Determination of bond strength of a single thread of cord with rubber is an exceptionally widely used type of testing in the rubber industry. It is based either on the principle of stripping of a single thread of cord from the rubber under pressure, or on the principle of pull-out of a thread of cord from a rubber specimen.


2018 ◽  
Vol 182 ◽  
pp. 02003
Author(s):  
Łukasz Muślewski ◽  
Leszek Knopik ◽  
Bogdan Landowski ◽  
Oleh Polishchuk

The problems discussed in the study are connected with evaluation of complex technical systems functioning, in particular, transport systems. It was assumed that evaluation of their functioning depends on fulfilment degree of selected criteria. Therefore, it is important to determine a set of criteria including their type, number and importance. Since the research object is a public city transport system considered to be a sociotechnical system of the type: human (driver) –machine (vehicle) and the environment <H-M-E>, the criteria to be used for the assessment must include behavior of humans, operation of transport means, and the environmental impact. Thus, selection and determination of importance of significant, time variable, measurable and independent characteristics whose values, in a given time moment or a given time interval, define fulfillment degree of the criteria provide the basis for evaluation of such systems functioning. The quality of technical systems functioning in time is assessed on the basis of the criteria fulfilment degree or comparison and classification of different systems of the same type. It needs to be emphasized that the choice of optimal methods for selection of relevant criteria and determination of their impact on the analyzed system functioning is the research subject of study.


2021 ◽  
Vol 29 ◽  
pp. 28-35
Author(s):  
Letícia Betânia Xavier Dias ◽  
Pedro Afonso de Melo Queiroz ◽  
Thaís Cardoso de Castro ◽  
Marco Antonio Moreira de Freitas ◽  
Érica Fernandes Leão-Araújo ◽  
...  

Our objective was to evaluate the occurrence of mechanical damage in chickpea seeds with the combination of two harvester speeds (2.5 and 3.5 km.h-1) and three adjustments for rotor rotation speeds (500, 700, and 850 rpm). Harvesting was carried out in a seed production field. Seeds were evaluated for purity, germination (G), first count (FC), germination speed index (GSI), hypochlorite, electrical conductivity, and tetrazolium tests. There was an effect of harvester speeds on seed physiological quality for the first count (FC), germination (G), germination speed index (GSI), especially when combining with high rotor rotation speed. In these cases, the speed of 2.5 km.h-1 resulted in lower values. This harvester speed also had worse results when combining with 850 rpm for purity and hypochlorite tests. The tetrazolium test was not efficient in identifying differences in seed quality. There was no significant interaction between harvester speeds and rotor rotation speeds for the conductivity test. Evaluating the harvester speed’s isolated effect (3.5 km.h-1), we identified problems in seed vigor due to the higher value of exudates in the electrical conductivity test. Low harvester speed (2.5 km.h-1) associated with high rotor rotation speeds (700 and 850 rpm) causes a reduction of the physical and physiological quality of seeds.


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