scholarly journals Culture Medium, Light Regime and Temperature Affect the Development of Sirosporium diffusum

2018 ◽  
Vol 10 (6) ◽  
pp. 310
Author(s):  
Tales Poletto ◽  
Marlove F. B. Muniz ◽  
Vinícius S. Fantinel ◽  
Renata F. Favaretto ◽  
Igor Poletto ◽  
...  
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Fungal Species ◽  
Light Regime ◽  
Leaf Spot Disease ◽  
Light Regimes ◽  
Physical Conditions ◽  
Randomized Design ◽  
Brown Leaf Spot ◽  
Brown Leaf

Sirosporium diffusum is the causal agent of the brown leaf spot disease on pecan trees that seriously damages the foliage of adult plants and seedlings. This fungal species is difficult to grow satisfactorily in a culture medium. Therefore, the aim of this study was to evaluate the effects of different physical conditions on the development of S. diffusum. In the first assay, eight culture media and five light regimes were combined, while in the second, the three treatments that promoted highest sporulation were combined with three temperatures. The trials were conducted in a two-factorial arrangement in a fully randomized design with six replicates. V8, V8CaCO3, and CA media under a 24-h photoperiod produced the highest respective sporulations: 29 × 104, 35 × 104, and 41 × 104 conidia ml-1. The best temperature for sporulation was 20±1 °C for all culture media, especially V8CaCO3 and CA. The best artificial conditions for obtaining good mycelial growth and sporulation consisted of a photoperiod of 24 h, temperature of 20±1 °C and V8CaCO3 or CA culture medium.

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

scholarly journals In vitro growth of Cattleya nobilior Rchb. f.: culture media, sealing systems and irradiance

2021 ◽  
Vol 51 ◽  
Author(s):  
Kaliana Gottschalk de Freitas ◽  
José Carlos Sorgato ◽  
Jackeline Schultz Soares ◽  
Luan Marlon Ribeiro
Keyword(s):  
Plant Height ◽  
Natural Ventilation ◽  
Culture Medium ◽  
Culture Media ◽  
Ventilation System ◽  
Light Condition ◽  
Leaf Length ◽  
In Vitro Growth ◽  
Randomized Design

ABSTRACT Some factors may influence the in vitro culture of native orchids, especially the culture medium, sealing system and light condition. This study aimed to evaluate the addition of banana pulp and peel in the formulation of culture media, sealing system and irradiance, in the in vitro growth of Cattleya nobilior Rchb. f. A completely randomized design was used in a factorial scheme with five culture media [Murashige & Skoog supplemented or not with banana pulp or peel (100 or 200g L-1)], two light conditions [3,000 K LED lamps (43 µmol m-2 s-1 or 86 µmol m-2 s-1)] and two sealing systems (natural and conventional ventilation), with six replicates of one vial each. The plant height, diameter of the largest pseudobulb, length of the largest root and of the largest leaf, fresh weight and number of leaves, roots and shoots were evaluated. The use of both the banana pulp and peel as additives in the culture medium formulation promoted the C. nobilior growth. The natural ventilation system increased the plant height, pseudobulb diameter and leaf length, while the conventional system promoted tillering.

Phosphite-based Products in the In vitro Colletotrichum musae Control

2019 ◽  
pp. 1-9
Author(s):  
Maria Luísa Mendes Rodrigues ◽  
Edson Hiydu Mizobutsi ◽  
Paola Junayra Lima Prates ◽  
Paula Virgínia Leite Duarte ◽  
Regina Cássia Ferreira Ribeiro ◽  
...  
Keyword(s):  
Mycelial Growth ◽  
Culture Medium ◽  
Culture Media ◽  
Petri Dish ◽  
State University ◽  
Randomized Design ◽  
Colletotrichum Musae ◽  
Study Laboratory ◽  
Vitro Effect

Aims: The aim of this study was to evaluate the in vitro effect of different phosphite formulations and concentrations on the development of Colletotrichum musae. Sample: to evaluate the inhibition of germination, mycelial growth and sporulation of Colletotrichum musae. Study Design:  Treatments were conducted in a completely randomized design, with 4 replicates, each replicate consisting of 1 Petri dish. Place and Duration of Study:  Laboratory of Post-Harvest Pathology, State University of Montes Claros, between March and October 2017. Methodology: Three different phosphite formulations were used: FCu1 (4% Cu + 20% P2O5), FCu2 (4% Cu + 22% P2O5) at concentrations of 0.5;1.0; 1.5 and 2.0 mL L-1 and FK (42% P2O5 + 27.7% K2O) at concentrations of 0.5; 1.0; 1.5 and 2.0 mg.L-1. Products were incorporated into the respective culture media. Culture medium alone and culture medium + imazalil were used as controls. Petri dishes were housed in BOD chamber at 25°C under a 12 hours photoperiod. Results: Results were submitted to analysis of variance and regression, and means were compared by the Tukey test (P <0.05). Control was compared to the other treatments by the Dunnet's test (P <0.05). Among the tested phosphite formulations, copper and potassium phosphites were found to reduce the mycelial growth of Colletotrichum musae. FCu2 presents a fungicide-like effect from the concentration of 0.5 m.L-1 in the control of conidia production. As for the FCu1, a fungicide-like effect was observed in the control of germination from the concentration of 1.5 mL.L-1. Conclusion: A significant fungistatic effect was observed between the concentrations of the products in the mycelial growth, sporulation and germination obtaining control of up to 100% of the development of C. musae. Copper phosphites were as effective as fungicide in inhibiting fungal development.

scholarly journals Morphology and Molecular Characteristics of Alternaria sonchi Causing Brown Leaf Spot on Sonchus asper in Korea

2021 ◽  
Vol 27 (3) ◽  
pp. 107-114
Author(s):  
Huan Luo ◽  
Myung Soo Park ◽  
Jun Myoung Yu
Keyword(s):  
Rna Polymerase Ii ◽  
Leaf Spot ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Leaf Spot Disease ◽  
Molecular Characteristics ◽  
Internal Transcribed Spacer Region ◽  
Sonchus Asper ◽  
Brown Leaf Spot ◽  
Brown Leaf

During a disease survey on weeds and minor cultivated crops in Korea, a brown leaf spot disease was observed on Sonchus asper. Leaf lesions were round or irregular in shape, and grayish brown to brown with a purple margin. In severe infection, lesions enlarged and coalesced, resulting in blighting of the leaves. The isolates from these leaf lesions were identified as Alternaira sonchi based on morphological characteristics and phylogenetic analyses of Internal transcribed spacer region, Alternaria allergen a1, glyceraldehyde 3-phosphate dehydrogenase, RNA polymerase II, and translation elongation factor genes. This study provides a comprehensive description of the morphological characteristics and phylogenetical traits of A. sonchi causing brown leaf spot on S. asper in Korea.

scholarly journals First Report of Alternaria Alternata Causing Brown Leaf Spot on Apricot (Prunus Armeniaca) in Karbala Province of Iraq

2021 ◽  
Vol 910 (1) ◽  
pp. 012080
Author(s):  
Zeinab L. Hameed ◽  
Adnan A. Lahuf ◽  
Mundher T. Jasim ◽  
Hassan M. Mohsen ◽  
Bahaa J. Kadim ◽  
...  
Keyword(s):  
Alternaria Alternata ◽  
Leaf Spot ◽  
Causative Factor ◽  
Prunus Armeniaca ◽  
Leaf Spot Disease ◽  
First Report ◽  
Spot Disease ◽  
Brown Leaf Spot ◽  
Rdna Sequencing ◽  
Brown Leaf

Abstract During a survey in season 018, leaf spot symptoms were commonly observed on apricot (Prunus armeniaca) trees in the orchards of Al-Hussainiya district in Karbala Province of Iraq. The symptomatic leaves were gathered, the associated fungus was isolated and characterized relied on its morphological features and ITS-rDNA sequencing. The causative factor was found to be the fungus Alternaria alternata that caused distinguishable leaf spot symptoms on the inoculated leaves of apricot. Based on a review of previous references relatedof this disease in Iraq, this is the first report of the brown leaf spot disease caused by A. alternata in Karbala province of Iraq.

scholarly journals Domestication of Red Seaweed (Gelidium latifolium) in Different Culture Media

2020 ◽  
Vol 25 (1) ◽  
pp. 39-44
Author(s):  
Andri Wijayanto ◽  
Ita Widowati ◽  
Tjahjo Winanto
Keyword(s):  
Research Laboratory ◽  
Culture Medium ◽  
Culture Media ◽  
Raw Material ◽  
Growth Media ◽  
Red Seaweed ◽  
Randomized Design ◽  
Gelidium Latifolium ◽  
Domestication Process ◽  
Completely Randomized Design

Gelidium latifolium is one of red seaweed types potentially can be developed as an industrial raw material. Since Gelidium is currently taken from ocean, the availability of seaweed from aquaculture is necessary to overcome the small number of its availability in nature. In Indonesia, G. latifolium cultivation has not been carried out so that domestication is required. The use of macro and micro nutrients in growth media is essentially needed for the domestication process. Domestication requires fast media and place for growth. The purpose of this study is to determine the growth of biomass and the survival of G. latifolium in different culture media. The study was conducted in a semi-outdoor research laboratory. The method used in this research is laboratory experimental method and Completely Randomized Design (CRD) with the treatment applied using 3 types of culture media (Urea: Za: TSP) by comparison (A) 100: 50: 50% (2 g.L-1), (B) 75: 75: 50% (2 g.L-1) and (C) 75: 50: 75% (2 g.L-1), with 3 replications. The seaweed was kept in 10 L of water in aeration equipped aquarium and filled with 10 g of G. latifolium on each treatment. The best growth rate of G. latifolium biomass is 5.67± 0.58 g and 100±0% are survived in C culture medium with a concentration of 75% Urea: 50% ZA: 75% TSP (2 g.L-1).

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