scholarly journals Effect of a 50-Hz Magnetic Field on the Degradation of Plasmid in the Presence of Cu(II) Ions and Hydrogen Peroxide

2017 ◽  
Vol 9 (4) ◽  
pp. 40 ◽  
Author(s):  
Yuuichi Kawashima ◽  
Honoka Fujii ◽  
Maresuke Nakayama ◽  
Masahiko Hasegawa ◽  
Satoru Shimanuki ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Hydrogen Peroxide ◽  
Gel Electrophoresis ◽  
Dna Strand Breaks ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Strand Breaks ◽  
Line Frequency ◽  
Dna Strand ◽  
The Magnetic Field

Power-line frequency (50/60 Hz) magnetic fields enhance DNA strand breaks in the cell. In order to understand the molecular mechanism underlying this phenomenon, we analyzed the conversion of plasmid from the supercoiled to the linear form. This conversion is promoted by hydroxyl radical generated by the reaction between Cu2+ and H2O2. The plasmid pHSG298 was incubated with 1 mM Cu2+ and 1 mM H2O2 and exposed to a 1.2 mT, 50-Hz magnetic field for 30, 60, and 90 min. The conversion of supercoiled DNA to linear DNA was analyzed by agarose gel electrophoresis. We found that exposure to the magnetic field for 90 min significantly enhanced the degree of conversion.

Generation of free radical and hydrogen peroxide from 2-hydroxyemodin, a direct-acting mutagen, and dna strand breaks by active oxygen

1987 ◽  
Vol 37 (2) ◽  
pp. 149-156 ◽  
Author(s):  
M. Kodama ◽  
Y. Kamioka ◽  
T. Nakayama ◽  
C. Nagata ◽  
N. Morooka ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Free Radical ◽  
Dna Strand Breaks ◽  
Active Oxygen ◽  
Strand Breaks ◽  
Dna Strand ◽  
Direct Acting

Effect of 2.45 mT sinusoidal 50 Hz magnetic field onSaccharomyces cerevisiaestrains deficient in DNA strand breaks repair

2010 ◽  
Vol 86 (7) ◽  
pp. 602-611 ◽  
Author(s):  
Miguel J. Ruiz-Gómez ◽  
Francisco Sendra-Portero ◽  
Manuel Martínez-Morillo
Keyword(s):  
Magnetic Field ◽  
Dna Strand Breaks ◽  
Strand Breaks ◽  
Dna Strand
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