Detection of Genotoxic Insult as DNA strand breaks in fish blood cells by agarose gel electrophoresis

1994 ◽  
Vol 13 (7) ◽  
pp. 1023-1031 ◽  
Author(s):  
Christopher W. Theodorakis ◽  
Steven J. D'Surney ◽  
Lee R. Shugart
Keyword(s):  
Blood Cells ◽  
Gel Electrophoresis ◽  
Dna Strand Breaks ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Strand Breaks ◽  
Fish Blood ◽  
Dna Strand ◽  
Genotoxic Insult

scholarly journals Effect of a 50-Hz Magnetic Field on the Degradation of Plasmid in the Presence of Cu(II) Ions and Hydrogen Peroxide

2017 ◽  
Vol 9 (4) ◽  
pp. 40 ◽  
Author(s):  
Yuuichi Kawashima ◽  
Honoka Fujii ◽  
Maresuke Nakayama ◽  
Masahiko Hasegawa ◽  
Satoru Shimanuki ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Hydrogen Peroxide ◽  
Gel Electrophoresis ◽  
Dna Strand Breaks ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Strand Breaks ◽  
Line Frequency ◽  
Dna Strand ◽  
The Magnetic Field

Power-line frequency (50/60 Hz) magnetic fields enhance DNA strand breaks in the cell. In order to understand the molecular mechanism underlying this phenomenon, we analyzed the conversion of plasmid from the supercoiled to the linear form. This conversion is promoted by hydroxyl radical generated by the reaction between Cu2+ and H2O2. The plasmid pHSG298 was incubated with 1 mM Cu2+ and 1 mM H2O2 and exposed to a 1.2 mT, 50-Hz magnetic field for 30, 60, and 90 min. The conversion of supercoiled DNA to linear DNA was analyzed by agarose gel electrophoresis. We found that exposure to the magnetic field for 90 min significantly enhanced the degree of conversion.

Positive Association Between DNA Strand Breaks in Peripheral Blood Mononuclear Cells and Polyunsaturated Fatty Acids in Red Blood Cells From Women

2007 ◽  
Vol 59 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Audur Y. Thorlaksdottir ◽  
Jon J. Jonsson ◽  
Laufey Tryggvadottir ◽  
Gudrun V. Skuladottir ◽  
Anna L. Petursdottir ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Peripheral Blood Mononuclear Cells ◽  
Blood Cells ◽  
Mononuclear Cells ◽  
Positive Association ◽  
Dna Strand Breaks ◽  
Peripheral Blood Mononuclear ◽  
Strand Breaks ◽  
Blood Mononuclear Cells ◽  
Dna Strand

Detection of DNA strand breakage in a marine amphipod by agarose gel electrophoresis: exposure to X-rays and copper

Biomarkers ◽  
2002 ◽  
Vol 7 (6) ◽  
pp. 451-463 ◽  
Author(s):  
Filipe O. Costa ◽  
Teresa Neuparth ◽  
M. Helena Costa ◽  
Christopher W. Theodorakis ◽  
Lee R. Shugart
Keyword(s):  
Gel Electrophoresis ◽  
Agarose Gel ◽  
X Rays ◽  
Agarose Gel Electrophoresis ◽  
Marine Amphipod ◽  
Strand Breakage ◽  
Dna Strand ◽  
Dna Strand Breakage
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