scholarly journals Alcoholic Extract of Wrightia tinctoria leaves and Isolates there of afford DNA Protection in Post Irradiated Sprague Dawley Rats

2016 ◽  
Vol 8 (4) ◽  
pp. 398-405
Author(s):  
Sathianarayanan SN ◽  
Asha Jose ◽  
Ranganathan Balasubramanian ◽  
Diana Thomas ◽  
Ashitha Balakrishnan ◽  
...  
Keyword(s):  
Alcoholic Extract ◽  
Sprague Dawley ◽  
Dna Protection ◽  
Sprague Dawley Rats ◽  
Wrightia Tinctoria

Postcoital interceptive activity of Wrightia tinctoria in Sprague–Dawley rats: a preliminary study

Contraception ◽  
2008 ◽  
Vol 78 (3) ◽  
pp. 266-270 ◽  
Author(s):  
Govind Keshri ◽  
Sudhir Kumar ◽  
Dinesh Kumar Kulshreshtha ◽  
Siron Mani Rajendran ◽  
Man Mohan Singh
Keyword(s):  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Preliminary Study ◽  
Wrightia Tinctoria

scholarly journals Evaluation of Hepato-protective and Nephron-Protective Potential of Euphorbia nivulia Buch.-Ham. Against Carbon Tetrachloride-induced Toxicity in Sprague Dawley Rats

2021 ◽  
pp. 83-100
Author(s):  
Muhammad Younus ◽  
Muhammad Mohtasheemul Hasan ◽  
Khalil Ahmad ◽  
Ihsanul Haq ◽  
Rizwan Ahmad ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Low Dose ◽  
Positive Control ◽  
High Dose ◽  
Alcoholic Extract ◽  
Sprague Dawley ◽  
Pathological Study ◽  
Hepatic Cells ◽  
Sprague Dawley Rats ◽  
Dose Dependent

Background: Euphorbia nivulia Buch.-Ham (En) is one of the members of Euphorbiaceae family that is rich in phytochemicals including flavonoids, triterpenes and polyphenolics. Purpose: To evaluate hepato-nephronprotective potential of Euphorbia nivulia. Study Design: Sprague Dawley rats were used as animal models in the study. Methods: En hydro alcoholic extract was standardized and managed in high dose (300 mgkg−1 body weight (BW) and low dose  (150 mgkg−1 BW) to Sprague Dawley rats, administered with CCl4 (1mlkg−1BW). Silymarin (50 mgkg−1 BW) was taken as positive control. The treatments were given thrice a week. Consequently, blood and hepatic homogenates were collected after 4 weeks of treatment. While the situation of kidney was explored through measurement of serum creatinine, serum urea, sodium and albumin levels. Hepatic and renal samples of rats treated with both 150 and 300 mg/kg of the extract were used for tissue pathological study. Results: En extract revealed dose dependent moderate level of shelter against CCl4 intoxicated hepato-nephrotoxicity as directed from the acquired results. The decrease of the albumin levels by the maximum dose of the extract exceeded similar to that attained with Silymarin, and the protecting effects of the extract against oxidative destruction were evaluated. Examination of serum show significant (p < 0.05) elevation in the level of aspartate transaminase(AST), alkaline phosphatase(ALP), alanine transaminase(ALT), whereas decline were noted for albumin in CCl4 treated rats. Histopathological cuts and damages were seen in hepatic cells and kidney of rats managed by CCl4. But, co-administration of En extract, dose dependently, improved the CCl4-carried hepatic harms in these limits. Conclusion: These effects propose that the phyto-ingredients of En extract with known polyphenols were able to improve the oxidative stress brought along with CCl4 and may be a useful healing mediator to manage oxidative stress related disorders like hepato-nephro toxicity.

scholarly journals Toxicological evaluation of chronic oral administration of Melissa officinalis hydro-ethanol extract in Sprague-Dawley rats

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Mohammad Hashemnia ◽  
Farid Rezaei ◽  
Zahra Nikousefat ◽  
Maral Bahiraei
Keyword(s):  
Mononuclear Cells ◽  
Control Group ◽  
High Dose ◽  
Alcoholic Extract ◽  
Sprague Dawley ◽  
Melissa Officinalis ◽  
Medicinal Uses ◽  
Sprague Dawley Rats ◽  
Term Administration

Melissa officinalis is a plant that has been widely used as an herbal medicine in many countries. Unfortunately, despite the prevalent medicinal uses of the plant, there are no reports on the possible toxic effects of M. officinalis. This study was designed to evaluate the effect of long-term administration of hydro-alcoholic extract of M. officinalis on some biochemical and hematological parameters and histopathology of organs. Thirty Sprague-Dawley rats were allocated to three equal groups. The animals in groups A and B received 600 and 1200 mg/kg M. officinalis extract, respectively, for 30 days. The rats in group C were given gavaged saline as control. The animals were euthanized at the end of experiment and the blood samples were collected for biochemical and hematology analysis. Additionally, appropriate tissue samples were collected from kidney, liver, spleen, heart and lung for light microscopic examination. M. officinalis caused a significant increase in the alanine aminotransferase level in the treated rats. Although the increase in creatine phosphokinase and lactate dehydrogenase levels were observed in group A and B, respectively, but there were no significant differences. A significant decrease was observed in the total protein and albumin concentrations in serum of treated rats as compared to the control group. The creatinine concentrations were significantly higher in the group B when compared to the other groups. There were no significant differences in cholesterol, triglyceride and urea concentrations between all groups of rats. The main histopathologic findings in the liver were included hepatocyte degeneration, congestion and dilation of sinusoids, proliferation of bile ducts and infiltration of mononuclear cells around the portal area. Histopathologic examination of the kidneys showed a tubular degeneration and necrosis, tubular and glomerular atrophy and congestion. These lesions were more prominent in the high dose treated rats. The findings suggest that long-term administration of M. officinalis extract even at low doses induces hepatic and renal lesions in rats.

Ultrastructural Investigation of Spontaneous Pituitary Adenomas in Aging Sprague-Dawley Rats

1982 ◽  
Vol 40 ◽  
pp. 216-217
Author(s):  
D. J. McComb ◽  
J. Beri ◽  
F. Zak ◽  
K. Kovacs
Keyword(s):  
Microscopic Study ◽  
Pituitary Adenomas ◽  
Wistar Rats ◽  
Microscopic Level ◽  
Sprague Dawley ◽  
Prolactin Cells ◽  
Light Microscopic Level ◽  
Ultrastructural Investigation ◽  
Sprague Dawley Rats ◽  
Long Evans

Investigation of the spontaneous pituitary adenomas in rat have been limited mainly to light microscopic study. Furth et al. (1973) described them as chromophobic, secreting prolactin. Kovacs et al. (1977) in an ul trastructural investigation of adenomas of old female Long-Evans rats, found that they were composed of prolactin cells. Berkvens et al. (1980) using immunocytochemistry at the light microscopic level, demonstrated that some spontaneous tumors of old Wistar rats could contain GH, TSH or ACTH as well as PRL.

Early Development of Drug-Induced Hepatic Necrosis

1971 ◽  
Vol 29 ◽  
pp. 576-577
Author(s):  
F. G. Zaki ◽  
E. Detzi ◽  
C. H. Keysser
Keyword(s):  
Early Development ◽  
Hepatic Necrosis ◽  
Screening Tests ◽  
Dense Matrix ◽  
Sprague Dawley ◽  
Electron Microscopic ◽  
Drug Induced ◽  
Hepatocellular Damage ◽  
Sprague Dawley Rats ◽  
Microscopic Studies

This study represents the first in a series of investigations carried out to elucidate the mechanism(s) of early hepatocellular damage induced by drugs and other related compounds. During screening tests of CNS-active compounds in rats, it has been found that daily oral administration of one of these compounds at a dose level of 40 mg. per kg. of body weight induced diffuse massive hepatic necrosis within 7 weeks in Charles River Sprague Dawley rats of both sexes. Partial hepatectomy enhanced the development of this peculiar type of necrosis (3 weeks instead of 7) while treatment with phenobarbital prior to the administration of the drug delayed the appearance of necrosis but did not reduce its severity.Electron microscopic studies revealed that early development of this liver injury (2 days after the administration of the drug) appeared in the form of small dark osmiophilic vesicles located around the bile canaliculi of all hepatocytes (Fig. 1). These structures differed from the regular microbodies or the pericanalicular multivesicular bodies. They first appeared regularly rounded with electron dense matrix bound with a single membrane. After one week on the drug, these vesicles appeared vacuolated and resembled autophagosomes which soon developed whorls of concentric lamellae or cisterns characteristic of lysosomes (Fig. 2). These lysosomes were found, later on, scattered all over the hepatocytes.

Ultrastructural investigation of spontaneous pituitary gonadotroph cell adenomas in aging Sprague-Dawley rats

1983 ◽  
Vol 41 ◽  
pp. 702-703
Author(s):  
D. J. McComb ◽  
J. Beri ◽  
F. Zak ◽  
K. Kovacs
Keyword(s):  
Electron Microscopy ◽  
Animal Model ◽  
Epoxy Resin ◽  
Immunoelectron Microscopy ◽  
Tumor Type ◽  
Gonadal Function ◽  
Sprague Dawley ◽  
Male And Female ◽  
Reticulin Fibers ◽  
Sprague Dawley Rats

Gonadotroph cell adenomas of the pituitary are infrequent in human patients and are not invariably associated with altered gonadal function. To date, no animal model of this tumor type exists. Herein, we describe spontaneous gonadotroph cell adenomas in old male and female Sprague-Dawley rats by histology, immunocytology and electron microscopy.The material consisted of the pituitaries of 27 male and 38 female Sprague Dawley rats, all 26 months of age or older, removed at routine autopsy. Sections of formal in-fixed, paraffin-embedded tissue were stained with hematoxylin-phloxine-saffron (HPS), the PAS method and the Gordon-Sweet technique for the demonstration of reticulin fibers. For immunostaining, sections were exposed to anti-rat β-LH, anti-ratβ-TSH, anti-rat PRL, anti-rat GH and anti-rat ACTH 1-39. For electron microscopy, tissue was fixed in 2.5% glutaraldehyde, postfixed in 1% OsO4 and embedded in epoxy-resin. Tissue fixed in 10% formalin, embedded in epoxy resin without osmification, was used for immunoelectron microscopy.

Freeze-etch studies of epiphyseal growth plate cartilage reveal matrix vesicles associated with calcification

1978 ◽  
Vol 36 (2) ◽  
pp. 670-671
Author(s):  
Russell N. A. Cecil ◽  
H. Clarke Anderson
Keyword(s):  
Chromic Acid ◽  
Light And Electron Microscopy ◽  
Matrix Vesicles ◽  
Matrix Vesicle ◽  
Glycerol Solution ◽  
Sprague Dawley ◽  
Epiphyseal Growth Plate ◽  
Growth Plates ◽  
Sprague Dawley Rats ◽  
Tibial Epiphyseal

Unfixed proximal tibial epiphyseal growth plates were studied by freeze-etch to confirm the presence of extracellular calcifying matrix vesicles and to determine the substructure of matrix vesicle membranes as compared to plasma and other membranes of intact chondrocytes. Growth plates from 6-10 week old Sprague-Dawley rats were cut into 1x3 mm blocks whose long dimension was oriented either perpendicular or parallel to the long axis of the tibia. Some blocks were fixed at pH 7. 0 in 0. 2M cacodylate - buffered 2. 5% glutaraldehyde for 1 hour at 4ÅC. The blocks were immersed in 30% glycerol solution at 4ÅC for 1 hour, frozen in liquid nitrogen, and then fractured, etched for 2 minutes, and coated with platinum, carbon and 0. 2% Formvar solution. The replicas were cleaned with chromic acid, floated onto Formvar coated grids, and examined with a Phillips EM 300 electron microscope.Fixed and unfixed specimens appeared similar in ultrastructure. Chondrocytes, matrix, and matrix vesicles were identified. In specimens fractured parallel to the long axis of the tibia, the reserve, proliferative, hypertrophic, and calcifying zones could be discerned as described by light and electron microscopy.

Sarcolemmal permeability changes during early myocardial anoxia

1978 ◽  
Vol 36 (2) ◽  
pp. 642-643
Author(s):  
M. Ashraf ◽  
L. Landa ◽  
L. Nimmo ◽  
C. M. Bloor
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Coronary Artery Occlusion ◽  
Artery Occlusion ◽  
Cell Membrane Permeability ◽  
Enzyme Release ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Sarcolemmal Permeability ◽  
Membrane Changes

Following coronary artery occlusion, the myocardial cells lose intracellular enzymes that appear in the serum 3 hrs later. By this time the cells in the ischemic zone have already undergone irreversible changes, and the cell membrane permeability is variably altered in the ischemic cells. At certain stages or intervals the cell membrane changes, allowing release of cytoplasmic enzymes. To correlate the changes in cell membrane permeability with the enzyme release, we used colloidal lanthanum (La+++) as a histological permeability marker in the isolated perfused hearts. The hearts removed from sprague-Dawley rats were perfused with standard Krebs-Henseleit medium gassed with 95% O2 + 5% CO2. The hypoxic medium contained mannitol instead of dextrose and was bubbled with 95% N2 + 5% CO2. The final osmolarity of the medium was 295 M osmol, pH 7. 4.

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