scholarly journals Effect of pioglitazone, quercetin and hydroxy citric acid on inflammatory markers in experimentally induced non alcoholic steatohepatitis (NASH).

2015 ◽  
Vol 49 (2) ◽  
pp. 152-157
Author(s):  
Surapaneni Krishna Mohan ◽  
Mallika Jainu
Keyword(s):  
Citric Acid ◽  
Inflammatory Markers ◽  
Alcoholic Steatohepatitis ◽  
Experimentally Induced

scholarly journals Biochemical alterations of inflammatory markers in experimentally induced diabetes in rats

2016 ◽  
Vol 30 (1) ◽  
pp. 29-38
Author(s):  
El-Regily F.Y. ◽  
El-Hindawy M. ◽  
Sadek M. ◽  
Abd El-Maksoud A.
Keyword(s):  
Inflammatory Markers ◽  
Biochemical Alterations ◽  
Experimentally Induced

Antrodia Cinnamomea Attenuates Non-Alcoholic Steatohepatitis by Suppressing NLRP3 Inflammasome Activation In Vitro and In Vivo

2020 ◽  
Vol 48 (08) ◽  
pp. 1859-1874
Author(s):  
I-Chuan Yen ◽  
Jung-Chun Lin ◽  
Yu Chen ◽  
Qian-Wen Tu ◽  
Shih-Yu Lee
Keyword(s):  
Nlrp3 Inflammasome ◽  
Inflammatory Markers ◽  
Inflammasome Activation ◽  
In Vivo Models ◽  
Antrodia Cinnamomea ◽  
Alcoholic Steatohepatitis ◽  
Stress Markers ◽  
Nlrp3 Inflammasome Activation

Blockade of the NOD-like receptor protein 3 (NLRP3) inflammasome has been shown to be effective in halting the progression of non-alcoholic steatohepatitis (NASH). Antrodia cinnamomea is a well-known indigenous medicine used by Taiwanese aboriginal tribes. However, its effect on NASH remains unclear. This study aimed to examine the mechanistic insight of Antrodia cinnamomea extract (ACE) in both in vitro and in vivo models of NASH. Murine RAW264.7 macrophages and human hepatocellular carcinoma HepG2 cells were treated with the indicated concentration of ACE 30 minutes prior to stimulation with lipopolysaccharide (LPS) for 24 h. Levels of inflammatory markers, NLRP3 inflammasome, components, and endoplasmic reticulum (ER) stress markers were analyzed by Western blotting. For the in vivo experiments, male C57BL/6 mice weighing 21–25 g were fed a methionine/choline deficient (MCD) diet along with vehicle or ACE (100 mg/kg) for 10 consecutive days. The serum glutamate pyruvate transaminase (SGPT) levels of the mice were measured. The liver tissues from the mice underwent histological analysis (hematoxylin and eosin staining), and the levels of inflammatory markers, NLRP3 inflammasome components, and autophagy-related proteins were evaluated. ACE significantly inhibited NLRP3 inflammasome activation in vitro and in vivo. In addition, ACE attenuated the severity of MCD-induced steatohepatitis, reduced the levels of oxidative stress markers, and ameliorated inflammatory responses, but restored autophagic flux. Based on these findings, Antrodia cinnamomea could be developed into an anti-non-alcoholic fatty liver disease/NASH agent.

Experimental Amyloidosis Induced by Immune Complex

1971 ◽  
Vol 29 ◽  
pp. 582-583
Author(s):  
A. Kawaoi
Keyword(s):  
Immune Complex ◽  
Systemic Amyloidosis ◽  
Human Diseases ◽  
Experimental Amyloidosis ◽  
Freund’S Complete Adjuvant ◽  
Freund's Complete Adjuvant ◽  
Immunological Approach ◽  
Experimentally Induced

Numbers of immunological approach have been made to the amyloidosis through the variety of predisposing human diseases and the experimentally induced animals by the greater number of agents. The results suggest an important role of impaired immunity involving both humoral and cell-mediated aspects.Recently the author has succeeded in producing amyloidosis in the rabbits and mice by the injections of immune complex of heat denatured DNA.The aim of this report is to demonstrate the details of the ultrastructure of the amyloidosis induced by heterologous insoluble immune complex. Eleven of twelve mice, dd strain, subcutaneously injected twice a week with Freund's complete adjuvant and four of seven animals intraperitonially injected developed systemic amyloidosis two months later from the initial injections. The spleens were electron microscopically observed.

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