scholarly journals Derivation and basic characterization of colorectal carcinoma primary cell lines

2017 ◽  
Vol 161 (4) ◽  
pp. 360-368 ◽  
Author(s):  
Lukas Krbal ◽  
Jiri Soukup ◽  
Stanislav John ◽  
Veronika Hanusova
2012 ◽  
Vol 187 (6) ◽  
pp. 2236-2242 ◽  
Author(s):  
Carsten Maik Naumann ◽  
Jan Sperveslage ◽  
Moritz F. Hamann ◽  
Ivo Leuschner ◽  
Linda Weder ◽  
...  

2020 ◽  
Vol 9 (3) ◽  
pp. 1698-1710
Author(s):  
Feiling Feng ◽  
Chuncui Huang ◽  
Mingjia Xiao ◽  
Huizhen Wang ◽  
Qingxiang Gao ◽  
...  

2019 ◽  
Vol 68 (3) ◽  
Author(s):  
David A. Trejo-Remigio ◽  
Luis F. Jacinto-Alemán ◽  
Elba R. Leyva-Huerta ◽  
Bogdan R. Navarro-Bustos ◽  
Javier Portilla-Robertson

1985 ◽  
Vol 5 (4) ◽  
pp. 642-648 ◽  
Author(s):  
J A Small ◽  
D G Blair ◽  
S D Showalter ◽  
G A Scangos

Two plasmids, one containing the simian virus 40 (SV40) genome and the mouse metallothionein I gene and one containing the v-myc gene of avian myelocytomatosis virus MC29, were coinjected into mouse embryos. Of the 13 surviving mice, one, designated M13, contained both myc and SV40 sequences. This mouse developed a cranial bulge identified as a choroid plexus papilloma at 13 weeks and was subsequently sacrificed; tissue samples were taken for further analysis. Primary cell lines derived from these tissues contained both myc and SV40 DNA. No v-myc mRNA could be detected, although SV40 mRNA was present in all of the cell lines tested. T antigen also was expressed in all of the cell lines analyzed. These data suggest that SV40 expression was involved in the abnormalities of mouse M13 and was responsible for the transformed phenotype of the primary cell lines. Primary cell lines from this mouse were atypical in that the population rapidly became progressively more transformed with time in culture based on the following criteria: morphology, growth rate, and the ability to grow in soft agar and in serum-free medium. The data also suggest that factors present in the mouse regulated the ability of SV40 to oncogenically transform most cells and that in vitro culture of cells allowed them to escape those factors.


Author(s):  
Pınar Arslan ◽  
Begum Yurdakok-Dikmen ◽  
Saniye Cevher Ozeren ◽  
Ozgur Kuzukiran ◽  
Ayhan Filazi

Author(s):  
V Rey Vázquez ◽  
L Fernández Nevado ◽  
S Tirados Menéndez ◽  
Ó Estupiñán Sánchez ◽  
R Rodríguez González

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