scholarly journals Characterization of adherent primary cell lines from fresh human glioblastoma tissue, defining glial fibrillary acidic protein as a reliable marker in establishment of glioblastoma cell culture

2020 ◽  
Author(s):  
Susanne Grube ◽  
Diana Freitag ◽  
Rolf Kalff ◽  
Christian Ewald ◽  
Jan Walter
Keyword(s):  
Cell Culture ◽  
Glial Fibrillary Acidic Protein ◽  
Cell Lines ◽  
Acidic Protein ◽  
Primary Cell ◽  
Glioblastoma Cell ◽  
Human Glioblastoma ◽  
Primary Cell Lines ◽  
Reliable Marker

scholarly journals TMOD-01. CHARACTERIZATION OF PATIENT-DERIVED PRIMARY CELL LINES AND XENOGRAFTS FOR GLIOBLASTOMA

2019 ◽  
Vol 21 (Supplement_6) ◽  
pp. vi262-vi262 ◽  
Author(s):  
Noriyuki Kijima ◽  
Daisuke Kanematsu ◽  
Tomoko Shofuda ◽  
Masahiro Nonaka ◽  
Ryoichi Iwata ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Surface ◽  
Cell Lines ◽  
Primary Cell Culture ◽  
Surface Marker ◽  
Primary Cell ◽  
Cell Surface Marker ◽  
High Expression ◽  
Glioblastoma Patient ◽  
Primary Cell Lines

Abstract Patient-derived primary cell culture and xenograft are essential tools for translational research for glioblastoma. However, characteristics of each patient derived cell line and xenograft is not extensively studied. In this study, we aim to analyze the characteristics of our glioblastoma patient-derived cell lines and xenografts based on cell surface markers and their differentiation patterns. We have established 20 glioblastoma primary cell culture lines by serum free medium containing EGF and bFGF and found that primary cell culture lines could be classified based on the expression of CD133 and CD44. Four cell lines had high expression of both CD133 and CD44. Eleven cell lines had high expression of only CD44, three cell lines had high expression of only CD133, two cell lines had low expression of both CD133 and CD44. In addition when we induce differentiation, these cell lines showed differentiation to both glial and neuronal differentiation, but differentiation patterns were different depending on each cell line. Four cell lines showed predominant neuronal differentiation and others showed predominant glial differentiation. We next investigated in vivo characteristics of glioblastoma patient derived xenografts from these established cell lines. We have injected these cell lines into NOD/Shi-scid IL2Rγ KO mouse and histopathologically analyzed characteristics of xenografts. Each xenograft well recapitulated histological features of original patients’ tumors and tumor cells remarkably invade through subventricular zone. These results suggest that glioblastoma patient derived primary cell lines and xenografts have different characteristics of cell surface marker expressions and differentiation patterns, thus can classify these cell lines depending on cell surface marker expressions and differentiation patterns. Further analysis is needed to examine the biological importance of the differences in cell surface marker expressions and differentiation patterns.

In vitro and in vivo characterization of a novel hedgehog signaling antagonist in human glioblastoma cell lines

2012 ◽  
Vol 131 (2) ◽  
pp. E33-E44 ◽  
Author(s):  
Pietro Ferruzzi ◽  
Federica Mennillo ◽  
Antonella De Rosa ◽  
Cinzia Giordano ◽  
Marco Rossi ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hedgehog Signaling ◽  
Glioblastoma Cell ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cell ◽  
In Vivo Characterization ◽  
Glioblastoma Cell Lines

Establishment and Characterization of Human Glioblastoma Cell Lines in vitro and their Xenografts in Nude Mice

1989 ◽  
Vol 12 (4) ◽  
pp. 169-174 ◽  
Author(s):  
H. Fischer ◽  
W.J. Zeller ◽  
K. Schwechheimer ◽  
K.-J. Hutter ◽  
B. Wowra ◽  
...  
Keyword(s):  
Cell Lines ◽  
Nude Mice ◽  
Glioblastoma Cell ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cell ◽  
Glioblastoma Cell Lines

scholarly journals Isolation of Cancer Stem Cells from Three Human Glioblastoma Cell Lines: Characterization of Two Selected Clones

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e105166 ◽  
Author(s):  
Fortunata Iacopino ◽  
Cristiana Angelucci ◽  
Roberto Piacentini ◽  
Filippo Biamonte ◽  
Annunziato Mangiola ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Cell Lines ◽  
Glioblastoma Cell ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cell ◽  
Glioblastoma Cell Lines

scholarly journals Derivation and basic characterization of colorectal carcinoma primary cell lines

2017 ◽  
Vol 161 (4) ◽  
pp. 360-368 ◽  
Author(s):  
Lukas Krbal ◽  
Jiri Soukup ◽  
Stanislav John ◽  
Veronika Hanusova
Keyword(s):  
Colorectal Carcinoma ◽  
Cell Lines ◽  
Primary Cell ◽  
Primary Cell Lines

scholarly journals Development of Primary Cell Lines from Gill, Kidney, Spleen and Caudal Fin of Common Carp (Cyprinus carpio)

2020 ◽  
Vol 12 (1) ◽  
pp. 73
Author(s):  
Insariani Insariani ◽  
Trisniaty Trisniaty ◽  
Freddy Riatmono ◽  
Abdul Ghani
Keyword(s):  
Cell Culture ◽  
Cell Lines ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Primary Cell ◽  
Fish Cell ◽  
Caudal Fin ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio ◽  
Primary Cell Lines

HighlihgtDevelop primary cultures derived From  tissue tails fins, gills, kidney and spleen from local Indonesian carp (Cyprinus carpio).Primary culture cell with L15  Mediacell cultures  consist of two type Fibroblast-like and epithelial –like cell AbstractThe fish cell lines technology have been developed for the interests of the fisheries world. This study aimed at developing a primary cell line from gill, kidney, spleen, and caudal fin of a common carp (Cyprinus carpio). A healthy common carp weighing 20 g (~1 month) was collected from the Cijeruk Fish Seed Center, Bogor. The development of primary cell lines from the gill, fin, tail, kidney and spleen tissue was performed in cell culture medium Leibovitz’s L-15 supplemented with 20% serum fetal bovine, 250 IU Penicillin, 250 µg / ml kanamycin sulfate and 2Mm L-Glutamine, and incubated at 28°C. Primary cell lines of caudal fin and gill began to form a monolayer on day 17 after culture. While the development of cell lines from kidney and spleen, although the initiation of cells and cells spread on the surface into a monolayer, was not perfect; therefore, the passage was unable to be done. Microscopic observations and Giemsa staining showed primary cell lines of caudal fin and gill based on cell morphology consisted of two cell types, fibroblast-like cells and epithelial-like cells. The first passage was done on day 17 when the confluence was more than 50%. The next passage was carried out every 3 weeks when confluence reached 70% -80%. The primary cell culture of gill was successfully passaged as much as 72 and the caudal fin was successfully passed as much as 89 times over 7 years. These new cell lines can be further used to propagate fish viruses and other biotechnology assays.

Establishment and Characterization of Primary Cell Lines of Squamous Cell Carcinoma of the Penis and its Metastasis

2012 ◽  
Vol 187 (6) ◽  
pp. 2236-2242 ◽  
Author(s):  
Carsten Maik Naumann ◽  
Jan Sperveslage ◽  
Moritz F. Hamann ◽  
Ivo Leuschner ◽  
Linda Weder ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Lines ◽  
Squamous Cell ◽  
Primary Cell ◽  
Primary Cell Lines

scholarly journals Neural differentiation of glioblastoma cell lines via a herpes simplex virus thymidine kinase/ganciclovir system driven by a glial fibrillary acidic protein promoter

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0253008
Author(s):  
Elizabeth Wei-Chia Luo ◽  
Meng-Lin Liao ◽  
Chung-Liang Chien
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Glial Fibrillary Acidic Protein ◽  
Thymidine Kinase ◽  
Cell Lines ◽  
Neural Differentiation ◽  
Acidic Protein ◽  
Glioblastoma Cell ◽  
Glioblastoma Cells ◽  
Simplex Virus

Glioblastoma is a malignant brain tumor with poor prognosis that rapidly acquires resistance to available clinical treatments. The herpes simplex virus thymidine kinase/ganciclovir (HSVtk/GCV) system produces the selective elimination of HSVtk-positive cells and is a candidate for preclinical testing against glioblastoma via its ability to regulate proliferation and differentiation. Therefore, in this study, we aimed to establish a plasmid encoding the HSVtk/GCV system driven by a glial fibrillary acidic protein (GFAP) promoter and verify its possibility of neural differentiation of glioblastoma cell line under the GCV challenge. Four stable clones—N2A-pCMV-HSVtk, N2A-pGFAP-HSVtk, U251-pCMV-HSVtk, and U251-pGFAP-HSVtk—were established from neuronal N2A and glioblastoma U251 cell lines. In vitro GCV sensitivity was assessed by MTT assay for monitoring time- and dosage-dependent cytotoxicity. The capability for neural differentiation in stable glioblastoma clones during GCV treatment was assessed by performing immunocytochemistry for nestin, GFAP, and βIII-tubulin. Under GFAP promoter control, the U251 stable clone exhibited GCV sensitivity, while the neuronal N2A clones were nonreactive. During GCV treatment, cells underwent apoptosis on day 3 and dying cells were identified after day 5. Nestin was increasingly expressed in surviving cells, indicating that the population of neural stem-like cells was enriched. Lower levels of GFAP expression were detected in surviving cells. Furthermore, βIII-tubulin-positive neuron-like cells were identified after GCV treatment. This study established pGFAP-HSVtk-P2A-EGFP plasmids that successfully ablated GFAP-positive glioblastoma cells, but left neuronal N2A cells intact. These data suggest that the neural differentiation of glioblastoma cells can be promoted by treatment with the HSVtk/GCV system.

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