scholarly journals Biocompatibility of quantum dots (CdSe/ZnS ) in human amniotic membrane-derived mesenchymal stem cells in vitro

2015 ◽  
Vol 159 (2) ◽  
pp. 227-233 ◽  
Author(s):  
Gongping Wang ◽  
Guangwei Zeng ◽  
Caie Wang ◽  
Huasheng Wang ◽  
Bo Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Quantum Dots ◽  
Mesenchymal Stem Cells ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane

scholarly journals An In vitro porcine study of repearing articular cartilage with human amniotic membrane epithelial and mesenchymal stem cells

2012 ◽  
Vol 20 ◽  
pp. S273
Author(s):  
E. Muiños-López ◽  
S.M. Díaz-Prado ◽  
T. Hermida-Gómez ◽  
E. Rendal-Vázquez ◽  
I. Fuentes-Boquete ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Articular Cartilage ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane

Mesenchymal Stem Cells from Human Amniotic Membrane and Umbilical Cord Can Diminish Immunological Response in an in vitro Allograft Model

2016 ◽  
Vol 82 (3) ◽  
pp. 267-275 ◽  
Author(s):  
Filip A. Dabrowski ◽  
Anna Burdzinska ◽  
Agnieszka Kulesza ◽  
Marcin Chlebus ◽  
Beata Kaleta ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Amniotic Membrane ◽  
Immunological Response ◽  
Human Amniotic Membrane

scholarly journals Study of Human Amniotic Membrane Mesenchymal Stem Cells Using Gelatin and Alginate as Nontoxic Scaffolds

2019 ◽  
Vol 5 ◽  
pp. 1
Author(s):  
Nike Hendrijantini ◽  
Poedjo Hartono ◽  
Helen Susilowati ◽  
Cindy K. Hartono ◽  
Reni P. Daniati ◽  
...  
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Mesenchymal Stem Cells ◽  
Amniotic Membrane ◽  
Colorimetric Assay ◽  
Control Group ◽  
Human Amniotic Membrane ◽  
Average Percentage ◽  
Significant Difference

Perinatal mesenchymal stem cells (MSCs), for example, from amniotic membrane, have advantages over adult sources, such as bone marrow, in terms of ease of availability, cell naivety, tissue stem cell abundance, high capacity of proliferation, and less donor site morbidity, because it does not require invasive procedures. Natural polymer scaffolds, such as gelatin and alginate, are biocompatible. Combination of stem cells from amniotic membrane (hAMSCs) and gelatin or alginate as scaffold can be promising. However, cytotoxicity comparison of gelatin and alginate to hAMSCs has not been widely studied. This study was aimed to compare cytotoxicity of gelatin and alginate on hAMSCs in vitro. Isolation and culture were performed on hAMSCs of the healthy full-term pregnancy. In passage 4, Flow Cytometry CD90, CD105, and CD73 phenotype characterization was done. Colorimetric assay of 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) was performed to measure the cytotoxicity. There were three sample groups: (control group) hAMSCs with alpha-minimum essential medium (α-MEM) solution as control; (gelatin group) hAMSCs with gelatin; (alginate group) hAMSCs with alginate. Each group consisted of 12 samples. Flow cytometry of hAMSCs expressed 28.78% CD90, 36.95% CD105, and 44.41% CD73 surface markers. No sample depicted toxicity in either gelatin or alginate group, and this is indicated by the average percentage of living cells in gelatin 97.26% and in alginate 98.43%. No statistically significant difference (ρ=0.057) of cytotoxicity was found between gelatin and alginate to hAMSCs. Gelatin and alginate were nontoxic to hAMSCs in vitro.

scholarly journals Anti-Inflammatory and Anti-Fibrotic Effects of Human Amniotic Membrane Mesenchymal Stem Cells and Their Potential in Corneal Repair

2018 ◽  
Vol 7 (12) ◽  
pp. 906-917 ◽  
Author(s):  
Alejandro Navas ◽  
Fátima Sofía Magaña-Guerrero ◽  
Alfredo Domínguez-López ◽  
César Chávez-García ◽  
Graciela Partido ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Anti Inflammatory

Stable and Non-Disruptive In Vitro/In Vivo Labeling of Mesenchymal Stem Cells by Internalizing Quantum Dots

2008 ◽  
Vol 0 (ja) ◽  
pp. 081201062920099
Author(s):  
YOSHIMI OHYABU ◽  
ZEENIA KAUL ◽  
TOMOKAZU YOSHIOKA ◽  
KAZUKI INOUE ◽  
SHINSUKE SAKAI ◽  
...  
Keyword(s):  
Stem Cells ◽  
Quantum Dots ◽  
Mesenchymal Stem Cells ◽  
In Vivo Labeling

scholarly journals FGF-2-Induced Human Amniotic Mesenchymal Stem Cells Seeded on a Human Acellular Amniotic Membrane Scaffold Accelerated Tendon-to-Bone Healing in a Rabbit Extra-Articular Model

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Jun Zhang ◽  
Ziming Liu ◽  
Yuwan Li ◽  
Qi You ◽  
Jibin Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Amniotic Membrane ◽  
Bone Healing ◽  
Chondrogenic Differentiation ◽  
Biomechanical Analysis ◽  
Specific Marker ◽  
Amniotic Mesenchymal Stem Cells

Background. FGF-2 (basic fibroblast growth factor) has a positive effect on the proliferation and differentiation of many kinds of MSCs. Therefore, it represents an ideal molecule to facilitate tendon-to-bone healing. Nonetheless, no studies have investigated the application of FGF-2-induced human amniotic mesenchymal stem cells (hAMSCs) to accelerate tendon-to-bone healing in vivo. Objective. The purpose of this study was to explore the effect of FGF-2 on chondrogenic differentiation of hAMSCs in vitro and the effect of FGF-2-induced hAMSCs combined with a human acellular amniotic membrane (HAAM) scaffold on tendon-to-bone healing in vivo. Methods. In vitro, hAMSCs were transfected with a lentivirus carrying the FGF-2 gene, and the potential for chondrogenic differentiation of hAMSCs induced by the FGF-2 gene was assessed using immunofluorescence and toluidine blue (TB) staining. HAAM scaffold was prepared, and hematoxylin and eosin (HE) staining and scanning electron microscopy (SEM) were used to observe the microstructure of the HAAM scaffold. hAMSCs transfected with and without FGF-2 were seeded on the HAAM scaffold at a density of 3×105 cells/well. Immunofluorescence staining of vimentin and phalloidin staining were used to confirm cell adherence and growth on the HAAM scaffold. In vivo, the rabbit extra-articular tendon-to-bone healing model was created using the right hind limb of 40 New Zealand White rabbits. Grafts mimicking tendon-to-bone interface (TBI) injury were created and subjected to treatment with the HAAM scaffold loaded with FGF-2-induced hAMSCs, HAAM scaffold loaded with hAMSCs only, HAAM scaffold, and no special treatment. Macroscopic observation, imageological analysis, histological assessment, and biomechanical analysis were conducted to evaluate tendon-to-bone healing after 3 months. Results. In vitro, cartilage-specific marker staining was positive for the FGF-2 overexpression group. The HAAM scaffold displayed a netted structure and mass extracellular matrix structure. hAMSCs or hAMSCs transfected with FGF-2 survived on the HAAM scaffold and grew well. In vivo, the group treated with HAAM scaffold loaded with FGF-2-induced hAMSCs had the narrowest bone tunnel after three months as compared with other groups. In addition, macroscopic and histological scores were higher for this group than for the other groups, along with the best mechanical strength. Conclusion. hAMSCs transfected with FGF-2 combined with the HAAM scaffold could accelerate tendon-to-bone healing in a rabbit extra-articular model.

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