Mesenchymal Stem Cells from Human Amniotic Membrane and Umbilical Cord Can Diminish Immunological Response in an in vitro Allograft Model

2016 ◽  
Vol 82 (3) ◽  
pp. 267-275 ◽  
Author(s):  
Filip A. Dabrowski ◽  
Anna Burdzinska ◽  
Agnieszka Kulesza ◽  
Marcin Chlebus ◽  
Beata Kaleta ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Amniotic Membrane ◽  
Immunological Response ◽  
Human Amniotic Membrane

scholarly journals An In vitro porcine study of repearing articular cartilage with human amniotic membrane epithelial and mesenchymal stem cells

2012 ◽  
Vol 20 ◽  
pp. S273
Author(s):  
E. Muiños-López ◽  
S.M. Díaz-Prado ◽  
T. Hermida-Gómez ◽  
E. Rendal-Vázquez ◽  
I. Fuentes-Boquete ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Articular Cartilage ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane

scholarly journals Biocompatibility of quantum dots (CdSe/ZnS ) in human amniotic membrane-derived mesenchymal stem cells in vitro

2015 ◽  
Vol 159 (2) ◽  
pp. 227-233 ◽  
Author(s):  
Gongping Wang ◽  
Guangwei Zeng ◽  
Caie Wang ◽  
Huasheng Wang ◽  
Bo Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Quantum Dots ◽  
Mesenchymal Stem Cells ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane

scholarly journals Study of Human Amniotic Membrane Mesenchymal Stem Cells Using Gelatin and Alginate as Nontoxic Scaffolds

2019 ◽  
Vol 5 ◽  
pp. 1
Author(s):  
Nike Hendrijantini ◽  
Poedjo Hartono ◽  
Helen Susilowati ◽  
Cindy K. Hartono ◽  
Reni P. Daniati ◽  
...  
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Mesenchymal Stem Cells ◽  
Amniotic Membrane ◽  
Colorimetric Assay ◽  
Control Group ◽  
Human Amniotic Membrane ◽  
Average Percentage ◽  
Significant Difference

Perinatal mesenchymal stem cells (MSCs), for example, from amniotic membrane, have advantages over adult sources, such as bone marrow, in terms of ease of availability, cell naivety, tissue stem cell abundance, high capacity of proliferation, and less donor site morbidity, because it does not require invasive procedures. Natural polymer scaffolds, such as gelatin and alginate, are biocompatible. Combination of stem cells from amniotic membrane (hAMSCs) and gelatin or alginate as scaffold can be promising. However, cytotoxicity comparison of gelatin and alginate to hAMSCs has not been widely studied. This study was aimed to compare cytotoxicity of gelatin and alginate on hAMSCs in vitro. Isolation and culture were performed on hAMSCs of the healthy full-term pregnancy. In passage 4, Flow Cytometry CD90, CD105, and CD73 phenotype characterization was done. Colorimetric assay of 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) was performed to measure the cytotoxicity. There were three sample groups: (control group) hAMSCs with alpha-minimum essential medium (α-MEM) solution as control; (gelatin group) hAMSCs with gelatin; (alginate group) hAMSCs with alginate. Each group consisted of 12 samples. Flow cytometry of hAMSCs expressed 28.78% CD90, 36.95% CD105, and 44.41% CD73 surface markers. No sample depicted toxicity in either gelatin or alginate group, and this is indicated by the average percentage of living cells in gelatin 97.26% and in alginate 98.43%. No statistically significant difference (ρ=0.057) of cytotoxicity was found between gelatin and alginate to hAMSCs. Gelatin and alginate were nontoxic to hAMSCs in vitro.

scholarly journals Microvesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Stimulated by Hypoxia Promote Angiogenesis Both In Vitro and In Vivo

2012 ◽  
Vol 21 (18) ◽  
pp. 3289-3297 ◽  
Author(s):  
Hong-Chao Zhang ◽  
Xin-Bin Liu ◽  
Shu Huang ◽  
Xiao-Yun Bi ◽  
Heng-Xiang Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Human Umbilical Cord

Taurine Promotes the Cartilaginous Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Vitro

2017 ◽  
Vol 42 (8) ◽  
pp. 2344-2353 ◽  
Author(s):  
Xiuhua Yao ◽  
Huiling Huang ◽  
Zhou Li ◽  
Xiaohua Liu ◽  
Weijia Fan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Human Umbilical Cord
Sign in / Sign up

Export Citation Format

Share Document