scholarly journals Effect of progesterone-carbachol derivative on perfusion pressure and coronary resistance in isolated rat heart: via activation of the M2 muscarinic receptor

2014 ◽  
Vol 158 (1) ◽  
pp. 073-079 ◽  
Author(s):  
Lauro Figueroa-Valverde ◽  
Francisco Diaz-Cedillo ◽  
Elodia Garcia-Cervera ◽  
Eduardo Pool Gomez ◽  
Maria Lopez-Ramos
Keyword(s):  
Muscarinic Receptor ◽  
Rat Heart ◽  
Perfusion Pressure ◽  
Isolated Rat Heart ◽  
Coronary Resistance ◽  
Isolated Rat

scholarly journals CHANGES INDUCED BY ESTRADIOL-ETHYLENEDIAMINE DERIVATIVE ON PERFUSION PRESSURE AND CORONARY RESISTANCE IN ISOLATED RAT HEART: L-TYPE CALCIUM CHANNEL

2011 ◽  
Vol 155 (1) ◽  
pp. 27-32 ◽  
Author(s):  
Lauro Figueroa-Valverde ◽  
Francisco Diaz-Cedillo ◽  
Maria Lopez-Ramos ◽  
Elodia Garcia-Cervera ◽  
Karen Quijano ◽  
...  
Keyword(s):  
Calcium Channel ◽  
Rat Heart ◽  
Perfusion Pressure ◽  
Isolated Rat Heart ◽  
Coronary Resistance ◽  
Isolated Rat

scholarly journals Design and Synthesis of Three Tetracyclic-Dione Derivatives and their Biological Activity on Perfusion Pressure Using an Isolated Rat Heart Model

2021 ◽  
Vol 12 (1) ◽  
pp. 253-263
Keyword(s):  
Biological Activity ◽  
Rat Heart ◽  
Perfusion Pressure ◽  
Isolated Rat Heart ◽  
Coronary Resistance ◽  
Heart Model ◽  
Design And Synthesis ◽  
Second Stage ◽  
Chemical Strategies ◽  
Isolated Rat

Several tetracyclic derivatives have been prepared with different biological activity; however, there are few reports on the effects exerted by the tetracyclic derivatives on the cardiovascular system. The objective of this investigation was to prepare three tetracyclic-dione derivatives (compounds 3 to 5) to evaluate their biological activity on perfusion pressure and coronary resistance. The first stage was achieved by the synthesis of three tetracyclic‐dione analogs using some chemical strategies. The second stage involves evaluating biological activity from tetracyclic‐derivatives on perfusion pressure and coronary resistance using an isolated rat heart model. The results showed that only compound 5 increases perfusion pressure and coronary resistance compared with the control conditions. In conclusion, the biological activity of compound 5 exerted against perfusion pressure and coronary resistance depends on the functional groups involved in their chemical structure.

P561Acute effects of dronedarone and amiodarone on functional, morphological and oxidative stress parameters in isolated rat heart with hypertension

EP Europace ◽  
2020 ◽  
Vol 22 (Supplement_1) ◽  
Author(s):  
S Simovic ◽  
J Jeremic ◽  
G Davidovic ◽  
I Srejovic ◽  
S Mitrovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rat Heart ◽  
Structural Changes ◽  
Perfusion Pressure ◽  
Coronary Perfusion Pressure ◽  
Isolated Rat Heart ◽  
Coronary Perfusion ◽  
Acute Administration ◽  
Isolated Hearts ◽  
Isolated Rat

Abstract Introduction Amiodarone represents the most widely used antiarrhythmic drug, even though it has been shown that it has negative inotropic and lusitropic effect in healthy hears. On the other hand, dronedarone reduces the risk of recurrent atrial fibrillation, but with increased early mortality related to the worsening of heart failure. However, the mechanisms responsible for these fatal outcomes remain unclear and require further examinations.  Purpose To investigate acute, direct effects of Dronedarone and Amiodarone on cardiac contractility, coronary flow and oxidative stress parameters in isolated rat heart with hypertension. Methods  The present study was carried out on 18 isolated hearts of spontaneously hypertensive Wistar Kyoto male rats (6 weeks old, bodyweight 200 ± 10 g). After isolation, all hearts were retrogradely perfused according to Langendorff technique with a gradually increment of coronary perfusion pressure (CPP from 40 to 120 cm H2O) and randomly divided into 3 groups: Control (n = 6), Amiodarone (n = 6, isolated hearts perfused with Amiodarone in dose of 3 umol), Dronedarone (n = 6, isolated hearts perfused with Dronedarone in dose of 1.8 umol). During ex vivo protocol continuously were registered cardiac contractility parameters and coronary flow, while from collected coronary venous effluent markers of oxidative stress were measured. All hearts were then fixated and stained with Hematoxylin/eosin. Results  Dronedarone severely depressed the function of all cardiodynamic parameters of the heart compared with Amiodarone or Control while Amiodarone intensified the function of the isolated rat heart with hypertension compared to Control (dp/dt max mmHg/s at coronary perfusion pressure 120cmH2O Dronedarone vs. Amiodarone vs. Control 579.733 ± 202.27 vs. 3063.65 ± 467.93 vs. 2682.88 ± 368.75; p < 0.001. dp/dt min mmHg/s 120cmH2O -352.13 ± 204.65 vs. 1960 ± 242.21 vs. -1858.83 ± 118.23; p < 0.001. SLVP mmHg at CPP 120cmH20 27.8 ± 3.46 vs. 98.95 ± 11.78 vs. 71.45 ± 7.56; p < 0.001. DLVP mmHg at CPP 120cmH2O 6.32 ± 0.49 vs. 4.83 ± 0.54 vs. 0.85 ± 0.35; p < 0.001). Acute administration of Dronedarone decreased the level of NO2- and increased the level of H2O2 , while Amiodarone heightens O2- levels (O2- nmol/min g wt at coronary perfusion pressure 120cmH2O Dronedarone vs. Amiodarone vs. Control  28.62 ± 2.54 vs. 77.3 ± 8.86 vs. 31.72 ± 4.56; p < 0.001. H2O2 nmol/min g wt at CPP 120cmH2O 92.56 ± 11.65 vs. 48.63 ± 10.11 vs. 42.84 ± 84; p < 0.001. NO2- nmol/min g wt at CPP 120cmH2O 38.61 ± 4.94 vs.  82.28 ± 5.76 vs.  64.71 ± 3.51; p < 0.001). Pathohistological, structural changes were observed in both, experimental groups. Conclusions  Acute administration of Dronedarone depresses cardiac function in isolated, working rat heart with hypertension, with decreasing the NO2- levels, increasing the level of H2O2 and enhanced structural changes when compared to Amiodarone.

Effect of cholesterol feeding and gender on the response of the isolated rat heart to hypoxia

1982 ◽  
Vol 60 (12) ◽  
pp. 1526-1532
Author(s):  
Margaret P. Moffat ◽  
Morris Karmazyn ◽  
Naranjan S. Dhalla
Keyword(s):  
Rat Heart ◽  
Control Diet ◽  
Dietary Cholesterol ◽  
Early Period ◽  
Isolated Rat Heart ◽  
Female Rats ◽  
Male Rats ◽  
Substantial Contribution ◽  
Coronary Resistance ◽  
Isolated Rat

The effect of hypoxic perfusion on isolated hearts from male and female rats fed either a control or 2% cholesterol supplemented diet was studied. Initiation of hypoxia produced a rapid decline in rate of hearts from either male or female animals irrespective of diet regimen. However, hearts from male rats fed a control diet exhibited a significantly higher (P < 0.01) resting rate during normoxic perfusion when compared with hearts from cholesterol-fed animals. Hypoxia also produced a rapid loss of myocardial contractile force and this effect was influenced by neither diet treatment nor animal gender. Similarly, there was little change in time to peak height of developed tension due to diet. Contracture development during hypoxic perfusion was significantly higher in hearts from female rats fed a cholesterol-supplemented diet compared with hearts from control diet fed female animals, whereas no changes were observed due to diet in hearts from male animals. Coronary resistance was increased as a result of hypoxic perfusion. In hearts from cholesterol-fed male rats this effect was substantially attenuated. Conversely, cholesterol supplementation resulted in a higher (P < 0.05) coronary resistance in hearts from female animals either during normoxic or the early period of hypoxic perfusion. Prostacyclin synthesis as measured by immunoreactive 6-keto-PGF1α efflux did not differ between hearts from either male or female rats during normoxic perfusion. Hypoxia significantly reduced 6-keto-PGF1α efflux from hearts of cholesterol-fed male rats whereas it produced no effect in any other treatment category. Our results demonstrate a substantial contribution of dietary cholesterol and animal gender in the response of the isolated rat heart to hypoxic perfusion.

scholarly journals Glucagon Effects on Ischemic Vasodilatation in the Isolated Rat Heart

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Mirko Rosic ◽  
Suzana Pantovic ◽  
Gvozden Rosic ◽  
Aleksandra Tomic-Lucic ◽  
Tatjana Labudovic ◽  
...  
Keyword(s):  
Rat Heart ◽  
Perfusion Pressure ◽  
Coronary Perfusion Pressure ◽  
Isolated Rat Heart ◽  
Myocardial Reperfusion ◽  
Coronary Perfusion ◽  
Vasoactive Substances ◽  
Significant Attenuation ◽  
Biphasic Release ◽  
Isolated Rat

The myocardial reperfusion following ischemia leads to the ischemic vasodilation by affecting the release of various vasoactive substances, such as free radicals, NO, and histamine. In addition, some evidences suggest that glucagon itself may alter the release of those substances. In this study, we investigated the ischemic vasodilation of the isolated rat heart, as well as the concentrations of NO, TBARS, and histamine in the coronary venous effluent either in the presence or in the absence of glucagon. Our results showed that in the presence of glucagon, there was a faster restoration of coronary perfusion pressure during ischemic vasodilation compared to the absence of glucagon ( versus  s) with no apparent changes in TBARS concentration. The glucagon's administration leads to the decreased release of histamine by approximately 35%. Biphasic release of NO in the presence of glucagon initially showed augmentation by 60%, followed by the significant attenuation of 45%.

Effect of Hydrogen Sulfide on Reactions of Isolated Rat Heart under Volume Load and Ischemia-Reperfusion

2013 ◽  
Vol 4 (3) ◽  
pp. 201-212
Author(s):  
Tetyana V Shimanskaya ◽  
Yulia V. Goshovska ◽  
Olena M. Semenykhina ◽  
Vadim F. Sagach
Keyword(s):  
Hydrogen Sulfide ◽  
Rat Heart ◽  
Ischemia Reperfusion ◽  
Isolated Rat Heart ◽  
Volume Load ◽  
Isolated Rat
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