Effect of cholesterol feeding and gender on the response of the isolated rat heart to hypoxia

1982 ◽  
Vol 60 (12) ◽  
pp. 1526-1532
Author(s):  
Margaret P. Moffat ◽  
Morris Karmazyn ◽  
Naranjan S. Dhalla
Keyword(s):  
Rat Heart ◽  
Control Diet ◽  
Dietary Cholesterol ◽  
Early Period ◽  
Isolated Rat Heart ◽  
Female Rats ◽  
Male Rats ◽  
Substantial Contribution ◽  
Coronary Resistance ◽  
Isolated Rat

The effect of hypoxic perfusion on isolated hearts from male and female rats fed either a control or 2% cholesterol supplemented diet was studied. Initiation of hypoxia produced a rapid decline in rate of hearts from either male or female animals irrespective of diet regimen. However, hearts from male rats fed a control diet exhibited a significantly higher (P < 0.01) resting rate during normoxic perfusion when compared with hearts from cholesterol-fed animals. Hypoxia also produced a rapid loss of myocardial contractile force and this effect was influenced by neither diet treatment nor animal gender. Similarly, there was little change in time to peak height of developed tension due to diet. Contracture development during hypoxic perfusion was significantly higher in hearts from female rats fed a cholesterol-supplemented diet compared with hearts from control diet fed female animals, whereas no changes were observed due to diet in hearts from male animals. Coronary resistance was increased as a result of hypoxic perfusion. In hearts from cholesterol-fed male rats this effect was substantially attenuated. Conversely, cholesterol supplementation resulted in a higher (P < 0.05) coronary resistance in hearts from female animals either during normoxic or the early period of hypoxic perfusion. Prostacyclin synthesis as measured by immunoreactive 6-keto-PGF1α efflux did not differ between hearts from either male or female rats during normoxic perfusion. Hypoxia significantly reduced 6-keto-PGF1α efflux from hearts of cholesterol-fed male rats whereas it produced no effect in any other treatment category. Our results demonstrate a substantial contribution of dietary cholesterol and animal gender in the response of the isolated rat heart to hypoxic perfusion.

scholarly journals Effect of progesterone-carbachol derivative on perfusion pressure and coronary resistance in isolated rat heart: via activation of the M2 muscarinic receptor

2014 ◽  
Vol 158 (1) ◽  
pp. 073-079 ◽  
Author(s):  
Lauro Figueroa-Valverde ◽  
Francisco Diaz-Cedillo ◽  
Elodia Garcia-Cervera ◽  
Eduardo Pool Gomez ◽  
Maria Lopez-Ramos
Keyword(s):  
Muscarinic Receptor ◽  
Rat Heart ◽  
Perfusion Pressure ◽  
Isolated Rat Heart ◽  
Coronary Resistance ◽  
Isolated Rat

scholarly journals CHANGES INDUCED BY ESTRADIOL-ETHYLENEDIAMINE DERIVATIVE ON PERFUSION PRESSURE AND CORONARY RESISTANCE IN ISOLATED RAT HEART: L-TYPE CALCIUM CHANNEL

2011 ◽  
Vol 155 (1) ◽  
pp. 27-32 ◽  
Author(s):  
Lauro Figueroa-Valverde ◽  
Francisco Diaz-Cedillo ◽  
Maria Lopez-Ramos ◽  
Elodia Garcia-Cervera ◽  
Karen Quijano ◽  
...  
Keyword(s):  
Calcium Channel ◽  
Rat Heart ◽  
Perfusion Pressure ◽  
Isolated Rat Heart ◽  
Coronary Resistance ◽  
Isolated Rat

scholarly journals Design and Synthesis of Three Tetracyclic-Dione Derivatives and their Biological Activity on Perfusion Pressure Using an Isolated Rat Heart Model

2021 ◽  
Vol 12 (1) ◽  
pp. 253-263
Keyword(s):  
Biological Activity ◽  
Rat Heart ◽  
Perfusion Pressure ◽  
Isolated Rat Heart ◽  
Coronary Resistance ◽  
Heart Model ◽  
Design And Synthesis ◽  
Second Stage ◽  
Chemical Strategies ◽  
Isolated Rat

Several tetracyclic derivatives have been prepared with different biological activity; however, there are few reports on the effects exerted by the tetracyclic derivatives on the cardiovascular system. The objective of this investigation was to prepare three tetracyclic-dione derivatives (compounds 3 to 5) to evaluate their biological activity on perfusion pressure and coronary resistance. The first stage was achieved by the synthesis of three tetracyclic‐dione analogs using some chemical strategies. The second stage involves evaluating biological activity from tetracyclic‐derivatives on perfusion pressure and coronary resistance using an isolated rat heart model. The results showed that only compound 5 increases perfusion pressure and coronary resistance compared with the control conditions. In conclusion, the biological activity of compound 5 exerted against perfusion pressure and coronary resistance depends on the functional groups involved in their chemical structure.

scholarly journals The effects of lidocaine on bupivacaine-induced cardiotoxicity in the isolated rat heart

2010 ◽  
pp. S65-S69
Author(s):  
I Křikava ◽  
J Jarkovský ◽  
P Štourač ◽  
M Nováková ◽  
P Ševčík
Keyword(s):  
Rat Heart ◽  
Constant Pressure ◽  
Isolated Rat Heart ◽  
Male Rats ◽  
Interval Prolongation ◽  
Sensory Blockade ◽  
Intraventricular Conduction ◽  
Isolated Hearts ◽  
Long Acting ◽  
Isolated Rat

Bupivacaine is a widely used long-acting local anaesthetic. In clinical practice, a mixture of bupivacaine and lidocaine is often used in order to combine the faster onset of sensory blockade of lidocaine with more profound and longer duration of blockade by bupivacaine. The aim of this study was to compare the cardiotoxicity of large doses of bupivacaine and mixture of bupivacaine with lidocaine in the isolated rat heart and to estimate whether or not the addition of lidocaine in clinically relevant concentration increases bupivacaine-induced toxicity. Experiments were performed on 21 adult male rats divided into three groups: B (6 µg/ml bupivacaine), BL (6 µg/ml bupivacaine and 12 µg/ml lidocaine) and L (12 µg/ml lidocaine). The experiment consisted of three 30 min periods: stabilisation, perfusion and washout. The isolated hearts were perfused according to Langendorff with Krebs-Henseleit solution at constant pressure (80 mmHg) and 37 °C (CaCl2 1.25 mM) and the heart rate (based on RR interval assessment), PQ and QRS intervals were measured. The present study shows that the mixture of tested anaesthetics – bupivacaine and lidocaine – impairs the intraventricular conduction parameters (QRS interval prolongation) to a lesser extent than bupivacaine itself, and that this effect is marked mainly at the beginning of perfusion.

Effect of Hydrogen Sulfide on Reactions of Isolated Rat Heart under Volume Load and Ischemia-Reperfusion

2013 ◽  
Vol 4 (3) ◽  
pp. 201-212
Author(s):  
Tetyana V Shimanskaya ◽  
Yulia V. Goshovska ◽  
Olena M. Semenykhina ◽  
Vadim F. Sagach
Keyword(s):  
Hydrogen Sulfide ◽  
Rat Heart ◽  
Ischemia Reperfusion ◽  
Isolated Rat Heart ◽  
Volume Load ◽  
Isolated Rat

The effect of dexamethasone on mucosal immunity of uterine tissue during pregnancy in rat

2019 ◽  
Vol 20 (1) ◽  
pp. 75-84
Keyword(s):  
Early Pregnancy ◽  
Histopathological Examination ◽  
Early Period ◽  
Interleukin 10 ◽  
Treated Group ◽  
Female Rats ◽  
Male Rats ◽  
Mrna Levels ◽  
Uterine Tissue ◽  
Leukocytic Infiltration

Disturbances in early pregnancy immunity affect embryo development, endometrial receptivity, placental development, fetal growth and lead to subfertility, dexamethasone is a synthetic glucocorticoid used for treatment of various complications. Immune cells and cytokines were examined during the early pregnancy in twenty-four female rats and six male rats for mating. Rats were grouped into two group control and dexamethasone treated by a dose of 50µgm/kgm body weight daily starting from one week before mating and persisted for one week after pregnancy. Blood samples were collected from each rat at 5hrs and at 1,3,7 day of pregnancy. Extracted RNA was subjected to real time PCR to determine mRNA levels for immune related genes interleukin1a(IL1A) and interleukin 10(IL10). Histopathological examination was done to uterus in order to detect leukocyte infiltration in uterine tissue. Results showed that significant increase in white blood cell count mainly eosinophil at 5hrs and lymphocyte at three and seven day of pregnancy of dexamethasone treated group. Moreover, TNF, C-reactive protein and progesterone were increased mainly at seven day of pregnancy of dexamethasone treated group. Similarly, interleukin 1alpha and interleukin 10 significantly increased at 5hrs and one day of pregnancy of dexamethasone treated group. In contrast, serum levels of total antioxidant capacity and estrogen were decreased significantly at 5hrs and seven day in dexamethasone treated group. Histopathological examination of uterus revealed leukocytic infiltration especially neutrophil and few eosinophils at five hours and one day of gestation then eosinophil become absent at 3day and seven day of dexamethasone group. Epithelial height and uterine gland diameter significantly increased at 5hrs, three day and seven days of gestation of dexamethasone treated group. The present investigation demonstrated that using of dexamethasone by dose of 50µgm/kgm during early pregnancy had a conflicting impact on some immune cytokines and parameters and may reflect a harmful response of immune system toward early period of pregnancy

‘Cross Talk’ Between Opioid Peptide and Adrenergic Receptor Signaling in Isolated Rat Heart

Circulation ◽  
1997 ◽  
Vol 95 (8) ◽  
pp. 2122-2129 ◽  
Author(s):  
Salvatore Pepe ◽  
Rui-Ping Xiao ◽  
Charlene Hohl ◽  
Ruth Altschuld ◽  
Edward G. Lakatta
Keyword(s):  
Cross Talk ◽  
Rat Heart ◽  
Adrenergic Receptor ◽  
Opioid Peptide ◽  
Isolated Rat Heart ◽  
Receptor Signaling ◽  
Isolated Rat

Comparison of Polarized and Depolarized Arrest in the Isolated Rat Heart for Long-term Preservation

Circulation ◽  
1997 ◽  
Vol 96 (9) ◽  
pp. 3148-3156 ◽  
Author(s):  
A. K. Snabaitis ◽  
M. J. Shattock ◽  
D. J. Chambers
Keyword(s):  
Rat Heart ◽  
Isolated Rat Heart ◽  
Long Term Preservation ◽  
Isolated Rat
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