scholarly journals Evaluating the Antimicrobial Efficacy of Root Canal Irrigants Against Candida Albicans And Enterococcus Faecalis: in vitro study

2010 ◽  
Vol 31 (1) ◽  
pp. 47-52
Author(s):  
Ilgın Akçay ◽  
Berivan Tuğba Türk ◽  
Beyser Pişkin ◽  
Bilge Hakan Şen ◽  
Tansel Öztürk
Keyword(s):  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antimicrobial Efficacy ◽  
Root Canal Irrigants

scholarly journals Evaluation of Antimicrobial Efficacy of Lyophilized Platelet Lysate against Organisms Isolated from Infected Root Canals: An in vitro Study

2018 ◽  
Vol 1 (11) ◽  
pp. 355-360
Author(s):  
Arvind Kumar ◽  
T L Suganya
Keyword(s):  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
In Vitro Study ◽  
Platelet Lysate ◽  
Vitro Study ◽  
Antimicrobial Efficacy ◽  
Freeze Dried

INTRODUCTION: The technically demanding endodontic treatment sometimes faces failures because of various polymicrobial infections. Common organisms isolated from such infections are Enterococcus faecalis and Candida albicans. Several researches were made with various materials to eradicate such organisms. The study material used here is Lyophilized platelet rich plasma (L-PRP), which has been recently used in dentistry and other medical fields to promote hard and soft tissue regeneration.AIM: The aim of this in vitro study was to evaluate the antimicrobial efficacy of lyophilized platelet lysate (LPL) against microorganisms like Enterococcus Faecalis, Bacillus Subtilis and Candida Albicans.MATERIALS AND METHOD: Discarded blood samples were obtained from blood banks after centrifugation, sterility check and activation. After this, lyophilization of the platelets was carried out. The antimicrobial activity of this freeze dried powder was evaluated by the minimum inhibitory concentration method and then by the Agar disk diffusion method.RESULTS: Lyophilized platelet lysate (LPL) inhibited the growth of Enterococcus faecalis, Bacillus subtilis and Candida albicans which is commonly associated with failed root canal treatment.CONCLUSION: LPL has the potential to be effective against the organisms which causes failure of root canal treatment. This can be of a valuable property in tissue engineering.

scholarly journals Antimicrobial efficacy of a new tri-antibiotic combination against resistant endodontic pathogens: An in-vitro study

2020 ◽  
Vol 23 (4) ◽  
pp. 8p ◽  
Author(s):  
Prasanna T. Dahake ◽  
Sudhindra M Baliga
Keyword(s):  
Root Canal ◽  
Pathogenic Bacteria ◽  
Culture Media ◽  
In Vitro Study ◽  
Vitro Study ◽  
New Combination ◽  
Antimicrobial Efficacy ◽  
E Coli ◽  
Background Removal

Background: Removal of all the pathogenic bacteria from the root canal system is of prime importance for the success of endodontic therapy. Objective: The study aimed to determine the antimicrobial efficacy of three antibiotics and their new combination against selected endodontic pathogens. Methods: In this in-vitro study, we used bacterial strains associated with the refractory endodontic condition and determined MIC and MBC of Clindamycin (C), Metronidazole (M), Doxycycline (D) as well as their combination CMD. We cultured Candida Albicans, Pseudomonas Aeruginosa, Escherichia Coli, Enterococcus Faecalis, Streptococcus Mutans, Bacillus Subtilis subsp. spizizenii, Actinomyces Actinomycetemcomitans on selective culture media. We analyzed the data using paired 't' test, one-way ANOVA, and Tuckey's HSD post hoc test. Results: Clindamycin inhibited the growth of C. Albicans (90%) and S. Mutans (90%) significantly and P. Aeruginosa, E. Coli, E. Faecalis, B. Subtilis, and A. Actinomycetemcomitans were resistant to it. Metronidazole did not inhibit any of the bacteria. Doxycycline inhibited C. Albicans (90%), P. Aeruginosa (90%), and S. Mutans (90%) significantly while E. Coli, E. Faecalis, B. Subtilis, and A. Actinomycetemcomitans were resistant to it. The combination of CMD inhibited all the microbes significantly. However, at bactericidal concentrations of CMD, E. Faecalis (p = 0.024), B. Subtilis (p = 0.021) and A. Actinomycetemcomitans (p = 0.041) were eliminated significantly, while C. Albicans (p = 0.164), P. Aeruginosa (p = 0.489), E. Coli (p = 0.106) and S. Mutans (p = 0.121) showed resistance. Conclusion: Combination CMD can be used against resistant endodontic pathogens to achieve predictable endodontic results.KEYWORDSAntimicrobial agents; Clindamycin; Doxycycline; Metronidazole; Root canal therapy.    

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