scholarly journals Optical coherence tomography: a novel modality for scrotal imaging

2013 ◽  
Vol 3 (4) ◽  
pp. 319 ◽  
Author(s):  
Cole Davis ◽  
Wayne Kuang
Keyword(s):  
Optical Coherence Tomography ◽  
Testicular Biopsy ◽  
Prise En Charge ◽  
Optical Coherence ◽  
Nonobstructive Azoospermia ◽  
Sperm Retrieval ◽  
Tomographic Images ◽  
Conventional Histology ◽  
Scrotal Imaging ◽  
Tubule Diameter

Background: For patients with nonobstructive azoospermia,sperm retrieval rates remain modest. We describe the use ofoptical coherence tomography to improve retrieval rates and todecrease tissue destruction.Methods: Four patients underwent diagnostic testicular biopsyand imaging with the Niris optical coherence tomography de -vice. We performed a descriptive comparison between optic alcoherence tomographic images and conventional histology.Results: The measured seminiferous tubule diameter differed by16 μm between comparative imaging from optical coherencetomography and conventional histology using hematoxylin andeosin staining.Conclusion: We illustrate the usefulness of optical coherencetomography in the setting of testicular biopsy and the managementof nonobstructive azoospermia.Contexte : Chez les patients atteints d'azoospermie non obstructive,les taux de collecte de spermatozoïdes demeurent modestes.Nous décrivons le recours à une tomographie optiquecohérente pour améliorer les taux de collecte et réduire ladestruction tissulaire.Méthodes : Quatre patients ont subi une biopsie testiculaire diagnostiqueet une épreuve d'imagerie à l'aide d'un appareil Nirisde tomographie optique cohérente. Une comparaison descriptivea été effectuée entre les images obtenues par tomographieoptique cohérente et les résultats des épreuves histologiquesstandard.Résultats : La différence dans le diamètre des tubules séminifèresmesuré par tomographie optique cohérente et par coloration histologiqueà l'hématoxyline-éosine n'était que de 16 μm.Conclusion : Nous présentons une étude descriptive illustrant l’uti -lité de la tomographie optique cohérente pendant une biopsietesticulaire en vue de la prise en charge d'une azoospermie nonobstructive.

scholarly journals Optical Coherence Tomography as a Tool for Ocular Dynamics Estimation

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Damian Siedlecki ◽  
Waldemar Kowalik ◽  
Henryk Kasprzak
Keyword(s):  
Heart Rate ◽  
Optical Coherence Tomography ◽  
Spectral Analysis ◽  
High Speed ◽  
Anterior Segment ◽  
Power Spectra ◽  
Optical Coherence ◽  
Tomographic Images ◽  
Noninvasive Estimation ◽  
Ocular Hemodynamics

Purpose. The aim of the study is to demonstrate that the ocular dynamics of the anterior chamber of the eye can be estimated quantitatively by means of optical coherence tomography (OCT).Methods. A commercial high speed, high resolution optical coherence tomographer was used. The sequences of tomographic images of the iridocorneal angle of three subjects were captured and each image from the sequence was processed in MATLAB environment in order to detect and identify the contours of the cornea and iris. The data on pulsatile displacements of the cornea and iris and the changes of the depth of the gap between them were retrieved from the sequences. Finally, the spectral analysis of the changes of these parameters was performed.Results. The results of the temporal and spectral analysis manifest the ocular microfluctuation that might be associated with breathing (manifested by 0.25 Hz peak in the power spectra), heart rate (1–1.5 Hz peak), and ocular hemodynamics (3.75–4.5 Hz peak).Conclusions. This paper shows that the optical coherence tomography can be used as a tool for noninvasive estimation of the ocular dynamics of the anterior segment of the eye, but its usability in diagnostics of the ocular hemodynamics needs further investigations.

Sperm Retrieval in the Obstructive, Nonobstructive Azoospermic and Aspermic Patients

2019 ◽  
Author(s):  
Ryan Flannigan ◽  
Peter N. Schlegel ◽  
E. Darracott Vaughan Jr.
Keyword(s):  
Key Words ◽  
Testicular Biopsy ◽  
Obstructive Azoospermia ◽  
Male Factor Infertility ◽  
Nonobstructive Azoospermia ◽  
Male Factor ◽  
Sperm Retrieval ◽  
Diagnostic Investigation ◽  
Work Up ◽  
Defective Spermatogenesis

Sperm retrieval includes essential procedures in the treatment and management of male factor infertility. Appropriate diagnostic investigation is necessary to correctly identify the etiology of azoospermia among obstructive, nonobstructive (defective spermatogenesis), and aspermia. In this chapter, we discuss the necessary work-up of an individual presenting with azoospermia along with the relevant medical and surgical management to optimize success with surgical sperm retrieval. This review contains 7 figures, 2 tables, and 68 references.  Key Words: anejaculation, azoospermia, MESA, microTESE, nonobstructive azoospermia, obstructive azoospermia, PESA, TESE, testicular biopsy

Techniques for sperm recovery in assisted reproduction

1999 ◽  
Vol 7 (2) ◽  
pp. 131-139 ◽  
Author(s):  
Sarah K Girardi ◽  
Peter N Schlegel
Keyword(s):  
Assisted Reproduction ◽  
Testicular Biopsy ◽  
Surgical Reconstruction ◽  
Significant Heterogeneity ◽  
Obstructive Azoospermia ◽  
Donor Insemination ◽  
Nonobstructive Azoospermia ◽  
Sperm Retrieval ◽  
Donor Sperm ◽  
Sperm Aspiration

During the past decade, few fields in medicine have changed as dramatically as reproductive medicine and the treatment of male infertility. Whereas previously only men with obstructive azoospermia were candidates for treatment, either through surgical reconstruction or sperm aspiration, now even men with nonobstructive azoospermia are able to achieve pregnancies without having to resort to donor sperm. The extraordinary success of assisted reproduction after sperm retrieval for azoospermic men is the result of three important discoveries. First is the clinical observation that epididymal transit of sperm is not required for successful fertilization. Second is the recognition that significant heterogeneity in testicular biopsy specimens exists. Lastly is the advent of intracytoplasmic sperm injection (ICSI), which has enabled fertilization regardless of the degree of sperm impairment or retrieval source as long as sperm are viable. These three discoveries have enabled fertilizations and pregnancies for men previously referred for donor insemination or adoption, and have therefore broadened the indications for sperm retrieval. This review is intended to describe in detail the available techniques for the recovery of sperm, with emphasis on the latest technique, testicular microdissection for sperm retrieval in nonobstructive azoospermia.

scholarly journals In VivoNoninvasive Imaging of Healthy Lower Lip Mucosa: A Correlation Study between High-Definition Optical Coherence Tomography, Reflectance Confocal Microscopy, and Histology

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Alejandra García-Hernández ◽  
Rodrigo Roldán-Marín ◽  
Pablo Iglesias-Garcia ◽  
Josep Malvehy
Keyword(s):  
Optical Coherence Tomography ◽  
Confocal Microscopy ◽  
Morphological Changes ◽  
Imaging Techniques ◽  
Reflectance Confocal Microscopy ◽  
Optical Coherence ◽  
High Definition ◽  
Lower Lip ◽  
Conventional Histology

In recent years, technology has allowed the development of new diagnostic techniques which allow real-time,in vivo, noninvasive evaluation of morphological changes in tissue. This study compares and correlates the images and findings obtained by high-definition optical coherence tomography (HD-OCT) and reflectance confocal microscopy (RCM) with histology in normal healthy oral mucosa. The healthy lip mucosa of ten adult volunteers was imaged with HD-OCT and RCM. Each volunteer was systematically evaluated by RCM starting in the uppermost part of the epithelium down to the lamina propia. Afterwards, volunteers were examined with a commercially available full-field HD-OCT system using both the “slice” and the “en-face” mode. A “punch” biopsy of the lower lip mucosa was obtained and prepared for conventional histology. The architectural overview offered by “slice” mode HD-OCT correlates with histologic findings at low magnification. In the superficial uppermost layers of the epithelium, RCM imaging provided greater cellular detail than histology. As we deepened into the suprabasal layers, the findings are in accordance with physiological cellular differentiation and correlate with the images obtained from conventional histology. The combined use of these two novel non-invasive imaging techniques provides morphological imaging with sufficient resolution and penetration depth, resulting in quasihistological images.

scholarly journals Fourier spectrum analysis of full-field optical coherence tomography for tissue imaging

2015 ◽  
Vol 471 (2179) ◽  
pp. 20150099 ◽  
Author(s):  
Wanrong Gao
Keyword(s):  
Optical Coherence Tomography ◽  
Fourier Spectrum ◽  
Fractal Model ◽  
Tissue Imaging ◽  
Optical Coherence ◽  
Spatial Correlation Function ◽  
Full Field ◽  
Tomographic Images ◽  
En Face ◽  
Fourier Spectrum Analysis

We propose a model of the full-field optical coherence tomography (FFOCT) technique for tissue imaging, in which the fractal model of the spatial correlation function of the refractive index of tissue is employed to approximate tissue structure. The results may be helpful for correctly interpreting en face tomographic images obtained with FFOCT.

1086: Cystoscopic Optical Coherence Tomography in Diagnosis and Management of Clinical Bladder Tumors

2007 ◽  
Vol 177 (4S) ◽  
pp. 358-359 ◽  
Author(s):  
Christopher S. Lee ◽  
Alek Mishail ◽  
Jason M. Kim ◽  
Alexander Kirshenbaum ◽  
Howard L. Adler ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence ◽  
Bladder Tumors ◽  
Diagnosis And Management
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