scholarly journals Stimulation of Alkaline Phosphatase Activity by PGE2 through Induction of IGF-1 in Human Dental Pulp Cells

2004 ◽  
Vol 2 (1) ◽  
pp. 33-38 ◽  
Author(s):  
Maki Sakamoto ◽  
Kiyoshi Matsushima ◽  
Muneyoshi Yamazaki
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells ◽  
Stimulation Of

Odontogenic stimulation of human dental pulp cells with bioactive nanocomposite fiber

2014 ◽  
Vol 29 (6) ◽  
pp. 854-866 ◽  
Author(s):  
Ga-Hyun Kim ◽  
Yong-Duk Park ◽  
So-Youn Lee ◽  
Ahmed El-Fiqi ◽  
Jung-Ju Kim ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Nanocomposite Fiber ◽  
Human Dental Pulp ◽  
Pulp Cells ◽  
Stimulation Of

scholarly journals Nanotopological-tailored calcium phosphate cements for the odontogenic stimulation of human dental pulp stem cells through integrin signaling

RSC Advances ◽  
2015 ◽  
Vol 5 (78) ◽  
pp. 63363-63371 ◽  
Author(s):  
So-Youn Lee ◽  
Hyung-Mun Yun ◽  
Roman A. Perez ◽  
Sara Gallinetti ◽  
Maria-Pau Ginebra ◽  
...  
Keyword(s):  
Stem Cells ◽  
Calcium Phosphate ◽  
Dental Pulp ◽  
Integrin Signaling ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Calcium Phosphate Cements ◽  
Human Dental Pulp ◽  
Pulp Cells ◽  
Stimulation Of

Nanotopological-tailored calcium phosphate cements stimulate odontogenesis of human dental pulp cells through integrin-mediated signaling pathways.

scholarly journals Platelet-Rich Plasma Induces Autophagy and Promotes Regeneration in Human Dental Pulp Cells

2021 ◽  
Vol 9 ◽  
Author(s):  
Hanxin Xu ◽  
Fen Xu ◽  
Jiajia Zhao ◽  
Caixia Zhou ◽  
Jiarong Liu
Keyword(s):  
Alkaline Phosphatase ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Platelet Rich Plasma ◽  
Cellular Migration ◽  
Expression Level ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells

Regenerative endodontic procedures using autologous platelet-rich plasma (PRP) can improve the biologic outcome of treatment. However, its mechanism of action on improving pulp regeneration is not fully elucidated. Autophagy was recently shown to be related to tissue repair and osteogenesis. Therefore, the objective of this study was to investigate the effect of PRP in dental pulp regeneration and to elucidate the role of autophagy involved in this process. Human dental pulp cells (hDPCs) were isolated from healthy dental pulp and co-cultured with an increasing concentration of PRP. Cellular migration and proliferation were determined by scratch assay, transwell assay, and cell-counting kit 8 assay. Osteogenic differentiation was clarified by using alkaline phosphatase staining, alizarin red staining, and real-time polymerase chain reaction (RT-PCR) to measure the gene expression levels of alkaline phosphatase, collagen-1, osteocalcin, dentin matrix protein 1, and dentin sialophosphoprotein. Autophagic bodies were observed by transmission electron microscopy and the expression of autophagy marker light chain 3B (LC3B) was determined by immunofluorescence staining. The mRNA and protein expression level of LC3B and Beclin-1 were quantified by qRT-PCR and western blotting. The effect of PRP on cellular migration, proliferation, and osteogenic differentiation was further investigated in the milieu of autophagy activator, rapamycin, and inhibitor, 3-methyladenine. Results showed that PRP promoted cell migration, proliferation, and osteogenic differentiation. Autophagic bodies were strongly activated and the expression level of LC3B and Beclin-1 was significantly promoted by PRP. Autophagy inhibition suppressed PRP-induced hDPCs migration, proliferation, and osteogenic differentiation, whereas autophagy activator substantially augmented PRP-stimulated migration, proliferation, and differentiation. Taken together, these findings suggested that PRP could effectively promote regenerative potentials associated with autophagy.

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