scholarly journals Application of real-time polymerase chain reaction using species specific primer targeting on mitochondrial cytochrome-b gene for analysis of pork in meatball products

2019 ◽  
Vol 6 (2) ◽  
pp. 260 ◽  
Author(s):  
Salmah Orbayinah ◽  
Hari Widada ◽  
Adam Hermawan ◽  
Sismindari Sudjadi ◽  
Abdul Rohman
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Cytochrome B ◽  
Mitochondrial Cytochrome ◽  
Specific Primer ◽  
Chain Reaction ◽  
Mitochondrial Cytochrome B ◽  
Mitochondrial Cytochrome B Gene ◽  
Polymerase Chain ◽  
Species Specific

Detection of Intraspecific.DNA Sequence Variation in the Mitochondrial Cytochrome b Gene of Atlantic Cod (Gadus morhua) by the Polymerase Chain Reaction

1991 ◽  
Vol 48 (1) ◽  
pp. 48-52 ◽  
Author(s):  
Steven M. Carr ◽  
H. Dawn Marshall
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequence ◽  
Cytochrome B ◽  
Gadus Morhua ◽  
Atlantic Cod ◽  
Mitochondrial Cytochrome ◽  
Chain Reaction ◽  
Mitochondrial Cytochrome B ◽  
Mitochondrial Cytochrome B Gene ◽  
Polymerase Chain

We determined the DNA sequence of a portion of the mitochondrial cytochrome b gene for 55 Atlantic cod (Gadus morhua) from Norway and from 10 locations within the Northern Cod complex and adjacent stocks off Newfoundland. DNA was prepared for sequencing by the polymerase chain reaction (PCR). Eleven variable nucleotide positions within a 298 base region defined 12 genotypes. Genotype proportions differed significantly between Newfoundland and Norwegian populations: the majority genotype among Newfoundland populations was present in a minority of Norwegian cod. Newfoundland cod showed less genotypic diversity than those from the eastern Atlantic: nine genotypes were found among all 10 Newfoundland populations, as compared with seven genotypes within the single Norwegian population. An exception was an overwintering, inshore Newfoundland population that showed four genotypes among five fish. As in other vertebrates, third position synonymous transitions predominate over other types of nucleotide changes. However, two amino acid replacement substitutions occur among cod, and the ratio of purine transitions to pyrimidine transitions is significantly higher than in other species. The existence of DNA sequence polymorphism permits the various hypotheses of the distribution and differentiation of Newfoundland cod stocks to be tested, and points to the utility of PCR technology in fishery genetics.

A new Real Time PCR with species-specific primers from Plasmodium malariae/P. brasilianum mitochondrial cytochrome b gene

2020 ◽  
Vol 76 ◽  
pp. 102069
Author(s):  
Emilly Henrique dos Santos ◽  
Lidia Yamamoto ◽  
Wilson Domingues ◽  
Silvia Maria di Santi ◽  
Kelly Aparecida Kanunfre ◽  
...  
Keyword(s):  
Real Time ◽  
Cytochrome B ◽  
Real Time Pcr ◽  
Plasmodium Malariae ◽  
Cytochrome B Gene ◽  
Mitochondrial Cytochrome ◽  
Specific Primers ◽  
Mitochondrial Cytochrome B ◽  
Mitochondrial Cytochrome B Gene ◽  
Species Specific

scholarly journals Foreign Eel Species in the Natural Waters of Japan Detected by Polymerase Chain Reaction of Mitochondrial Cytochrome b Region

1999 ◽  
Vol 65 (5) ◽  
pp. 684-686 ◽  
Author(s):  
Huan Zhang ◽  
Naomi Mikawa ◽  
Yoshiaki Yamada ◽  
Noriyuki Horie ◽  
Akihiro Okamura ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cytochrome B ◽  
Natural Waters ◽  
Mitochondrial Cytochrome ◽  
Chain Reaction ◽  
Mitochondrial Cytochrome B ◽  
Polymerase Chain
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