Identification of Anchovy (Engraulis encrasicholusL.) and Gilt Sardine (Sardinella aurita) by Polymerase Chain Reaction, Sequence of Their Mitochondrial Cytochrome b Gene, and Restriction Analysis of Polymerase Chain Reaction Products in Semipreserves

2001 ◽  
Vol 49 (3) ◽  
pp. 1194-1199 ◽  
Author(s):  
Paola Sebastio ◽  
Paola Zanelli ◽  
Tauro Maria Neri
Keyword(s):  
Polymerase Chain Reaction ◽  
Restriction Analysis ◽  
Mitochondrial Cytochrome ◽  
Reaction Products ◽  
Reaction Sequence ◽  
Chain Reaction ◽  
Mitochondrial Cytochrome B ◽  
Mitochondrial Cytochrome B Gene ◽  
Polymerase Chain ◽  
Sardinella Aurita

Detection of Intraspecific.DNA Sequence Variation in the Mitochondrial Cytochrome b Gene of Atlantic Cod (Gadus morhua) by the Polymerase Chain Reaction

1991 ◽  
Vol 48 (1) ◽  
pp. 48-52 ◽  
Author(s):  
Steven M. Carr ◽  
H. Dawn Marshall
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequence ◽  
Cytochrome B ◽  
Gadus Morhua ◽  
Atlantic Cod ◽  
Mitochondrial Cytochrome ◽  
Chain Reaction ◽  
Mitochondrial Cytochrome B ◽  
Mitochondrial Cytochrome B Gene ◽  
Polymerase Chain

We determined the DNA sequence of a portion of the mitochondrial cytochrome b gene for 55 Atlantic cod (Gadus morhua) from Norway and from 10 locations within the Northern Cod complex and adjacent stocks off Newfoundland. DNA was prepared for sequencing by the polymerase chain reaction (PCR). Eleven variable nucleotide positions within a 298 base region defined 12 genotypes. Genotype proportions differed significantly between Newfoundland and Norwegian populations: the majority genotype among Newfoundland populations was present in a minority of Norwegian cod. Newfoundland cod showed less genotypic diversity than those from the eastern Atlantic: nine genotypes were found among all 10 Newfoundland populations, as compared with seven genotypes within the single Norwegian population. An exception was an overwintering, inshore Newfoundland population that showed four genotypes among five fish. As in other vertebrates, third position synonymous transitions predominate over other types of nucleotide changes. However, two amino acid replacement substitutions occur among cod, and the ratio of purine transitions to pyrimidine transitions is significantly higher than in other species. The existence of DNA sequence polymorphism permits the various hypotheses of the distribution and differentiation of Newfoundland cod stocks to be tested, and points to the utility of PCR technology in fishery genetics.

scholarly journals Foreign Eel Species in the Natural Waters of Japan Detected by Polymerase Chain Reaction of Mitochondrial Cytochrome b Region

1999 ◽  
Vol 65 (5) ◽  
pp. 684-686 ◽  
Author(s):  
Huan Zhang ◽  
Naomi Mikawa ◽  
Yoshiaki Yamada ◽  
Noriyuki Horie ◽  
Akihiro Okamura ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cytochrome B ◽  
Natural Waters ◽  
Mitochondrial Cytochrome ◽  
Chain Reaction ◽  
Mitochondrial Cytochrome B ◽  
Polymerase Chain

Identification of Thunnus Tuna Species by the Polymerase Chain Reaction and Direct Sequence Analysis of their Mitochondrial Cytochrome b Genes

1991 ◽  
Vol 48 (2) ◽  
pp. 309-317 ◽  
Author(s):  
Sylvia E. Bartlett ◽  
William S. Davidson
Keyword(s):  
Polymerase Chain Reaction ◽  
Cytochrome B ◽  
Genetic Relationships ◽  
Bluefin Tuna ◽  
Water Soluble ◽  
Mitochondrial Cytochrome ◽  
Chain Reaction ◽  
Species Identity ◽  
Mitochondrial Cytochrome B ◽  
Polymerase Chain

Four commercially important tuna species in the genus Thunnus are caught off the east coast of Canada. The harvest of bluefin tuna (T. thynnus) is regulated, but that of bigeye (T. obesus), yellowfin (T. albacares), or albacore (T. alalunga) is not. Enforcement of the regulations governing the biuefin fishery has been difficult because of the close genetic relationships among these species and the ease with which morphological characters may be removed once a fish has been landed. Isoelectric-focusing of water-soluble muscle proteins does not resolve these four tuna species beyond two groups: one consisting of bluefin and yellowfin and the other comprising bigeye and albacore. The polymerase chain reaction was used to amplify a 307 base pair segment of the mitochondrial cytochrome b gene from members of these species. There is intraspecific variation at this locus in each of the species. More importantly, there are differences between the four species and these genetic markers can be used to determine the species identity of an individual tuna with a high degree of confidence. This methodology should prove very useful for enforcing the regulations governing Canada's bluefin tuna fishing industry.

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