Comprehensive approach to pediatric kidney diseases in Guatemala

2015 ◽  
Vol 83 (2015) (S1) ◽  
pp. 82-84 ◽  
Author(s):  
Randall Lou-Meda
Keyword(s):  
Kidney Diseases ◽  
Comprehensive Approach ◽  
Pediatric Kidney Diseases

scholarly journals Enhancing clinical trial development for pediatric kidney diseases

2017 ◽  
Vol 82 (5) ◽  
pp. 727-732 ◽  
Author(s):  
H William Schnaper ◽  
Joseph T Flynn ◽  
Coleman Gross ◽  
Anne B Cropp ◽  
Bastian Dehmel ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Kidney Diseases ◽  
Pediatric Kidney Diseases

Epidemiology of Pediatric Kidney Diseases

1996 ◽  
Vol 25 (5) ◽  
pp. 288-296 ◽  
Author(s):  
Robert J Wyatt ◽  
Lori Kagy ◽  
Stephen B Kritchevsky
Keyword(s):  
Kidney Diseases ◽  
Pediatric Kidney Diseases

scholarly journals Kidney diseases in children – early diagnosis and prevention

PRILOZI ◽  
2016 ◽  
Vol 37 (1) ◽  
pp. 5-7
Author(s):  
Momir Polenakovic ◽  
Zoran Gucev ◽  
Velibor Tasic
Keyword(s):  
Urinary Tract ◽  
Early Diagnosis ◽  
Health Professionals ◽  
Kidney Diseases ◽  
Healthy Life ◽  
The World ◽  
Interactive Lectures ◽  
Healthy Life Style ◽  
The Media ◽  
Pediatric Kidney Diseases

Abstract Pediatric kidney diseases were in the focus of the World Kidney Day 2016. Macedonian pediatric nephrologists gave their contribution with public appearance in kindergartens, primary and secondary schools, with interactive lectures and discussion with the youngest about the kidney function, healthy life style and simple measures to prevent kidney and urinary tract diseases. Besides promotive appearance in the media, series of lectures were presented in front of the health professionals. The aim was to attract the attention of the professionals for early diagnosis and prevention of kidney disease. The action starts in utero, followed by early postnatal imaging and assessment, conservative treatment and in selected cases surgical treatment. The emphasis is on the multidisciplinary and comprehensive approach to children and adolescents with kidney diseases.

Nuclear Imaging in Pediatric Kidney Diseases

2018 ◽  
Vol 55 (7) ◽  
pp. 591-597 ◽  
Author(s):  
Rachita Singh Dhull ◽  
Aparna Joshi ◽  
Abhijeet Saha
Keyword(s):  
Kidney Diseases ◽  
Nuclear Imaging ◽  
Pediatric Kidney Diseases

scholarly journals Expression of Nephrin in Pediatric Kidney Diseases

2001 ◽  
Vol 12 (2) ◽  
pp. 289-296
Author(s):  
JAAKKO PATRAKKA ◽  
VESA RUOTSALAINEN ◽  
ILKKA KETOLA ◽  
CHRISTER HOLMBERG ◽  
MARKKU HEIKINHEIMO ◽  
...  
Keyword(s):  
Focal Segmental Glomerulosclerosis ◽  
Membranous Nephropathy ◽  
Staining Pattern ◽  
Kidney Diseases ◽  
Minimal Change ◽  
Slit Diaphragm ◽  
Segmental Glomerulosclerosis ◽  
Minimal Change Nephrosis ◽  
Pediatric Kidney Diseases

Abstract. Nephrin is a podocyte cell adhesion protein located at the slit diaphragm area of the kidney glomerulus. Mutations in the nephrin gene (NPHS1) lead to congenital nephrosis, suggesting that nephrin is essential for the glomerular filtration barrier. This prompted this study of the expression of nephrin in acquired pediatric kidney diseases usingin situhybridization and immunohistochemistry.In situhybridization for nephrin mRNA was performed in biopsy samples from patients with proteinuria caused by minimal change nephrosis, focal segmental glomerulosclerosis, and membranous nephropathy. The expression of nephrin mRNA was evaluated by grading the signal intensity visually and by counting the number of grains in separate glomeruli. No significant difference was observed in these samples as compared with controls. Immunostaining for nephrin was performed using antibodies directed against extra- and intracellular parts of the molecule. Nephrin staining gave a linear pattern along the glomerular capillary loops. In minimal change nephrosis, focal segmental glomerulosclerosis, and membranous nephropathy, the distribution of nephrin was similar to that in controls. In proliferative forms of glomerulonephritides (Henoch-Schönlein nephritis, IgA nephropathy, postinfectious and membranoproliferative glomerulonephritis), crescents and sclerotic lesions were negative for nephrin, and mesangial proliferation led to a scattered and sparse staining pattern. The staining pattern of nephrin was compared to that of ZO-1, a component of the cytoplasmic face of the slit diaphragm. The distributions of these two proteins in capillary tufts were similar in all disease entities studied. In conclusion, immunohistochemistry andin situhybridization did not reveal major alterations in the expression of nephrin in proteinuric kidney diseases in children. Further studies are needed for more precise evaluation of the role of nephrin in these diseases.

Immunolabelling of glomerular deposits in kidney biopsies routinely fixed in glutaraldehyde

1989 ◽  
Vol 47 ◽  
pp. 910-911
Author(s):  
Ś Lhoták ◽  
I. Alexopoulou ◽  
G. T. Simon
Keyword(s):  
Uv Light ◽  
Kidney Diseases ◽  
Light Chains ◽  
Microscopical Level ◽  
Accurate Identification ◽  
Light Microscopical Level ◽  
Dense Deposits ◽  
Cacodylate Buffer ◽  
Glomerular Deposits ◽  
Prognostic Importance

Various kidney diseases are characterized by the presence of dense deposits in the glomeruli. The type(s) of immunoglobulins (Igs) present in the dense deposits are characteristic of the disease. The accurate Identification of the deposits is therefore of utmost diagnostic and prognostic importance. Immunofluorescence (IF) used routinely at the light microscopical level is unable to detect and characterize small deposits found in early stages of glomerulonephritis. Although conventional TEM is able to localize such deposits, it is not capable of determining their nature. It was therefore attempted to immunolabel at EM level IgG, IgA IgM, C3, fibrinogen and kappa and lambda Ig light chains commonly found in glomerular deposits on routinely fixed ( 2% glutaraldehyde (GA) in 0.1M cacodylate buffer) kidney biopsies.The unosmicated tissue was embedded in LR White resin polymerized by UV light at -10°C. A postembedding immunogold technique was employed

Sign in / Sign up

Export Citation Format

Share Document