scholarly journals Effect of Immunosuppression on Newcastle Disease Virus Persistence in Ducks with Different Immune Status

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Lucy W. Njagi ◽  
Phillip N. Nyaga ◽  
Lilly C. Bebora ◽  
Paul G. Mbuthia ◽  
Uswege M. Minga
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Immune Status ◽  
Experimental Period ◽  
Hemagglutination Inhibition Test ◽  
Viral Excretion ◽  
Antibody Titres ◽  
Antibody Levels ◽  
Chicken Embryo Fibroblasts

This study was carried out to verify the possibility that ducks are sources of Newcastle disease (ND) virus infection for chickens in mixed flocks. Immunosuppressed (IS) and non immunosuppressed (NIS) birds, at three different antibody levels (medium, low and absent) were used; the titres having been induced through vaccination, and Immunosuppression done using dexamethazone. Each of the 3 respective groups was further divided into 2 groups of about 12 ducks each: one challenged with velogenic ND virus; the other not challenged. Selected ducks from all groups had their antibody titres monitored serially using hemagglutination inhibition test, while two birds from each of the challenged groups were killed and respective tissues processed for ND viral recovery, using chicken embryo fibroblasts. In general, antibody titres of IS and NIS challenged ducks were significantly higher than their unchallenged counterparts (P<0.05). Non-challenged pre-immunised ducks had a progressive decrease in antibody levels; non-immunised ducks did not seroconvert. Newcastle disease virus was isolated from livers and kidneys of the challenged ducks throughout the experimental period; indicating a possibility of viral excretion, especially when the birds are stressed. It, therefore, provides another possible model of viral circulation within mixed flocks.

scholarly journals Determination of serum antibody titres and immune status of layer flocks against Newcastle Disease virus at Chittagong district of Bangladesh

1970 ◽  
Vol 1 (2) ◽  
pp. 35-38 ◽  
Author(s):  
MA Kashem ◽  
M Parvej ◽  
MA Hashem ◽  
MM Moula ◽  
ASMG Kibria
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Immune Status ◽  
Haemagglutination Inhibition ◽  
Serum Antibody ◽  
Age Groups ◽  
Serum Samples ◽  
Specific Immunity ◽  
Antibody Titres

A study was conducted to assess the level of serum antibody titres and immune status of layer birds against Newcastle Disease virus by Haemagglutination Inhibition (HI) test in different areas of Chittagong district during November to December, 2010. Sixteen layer flocks were selected based on different ages of birds. A total of 235 serum samples were collected and tested at Microbiology laboratory of CVASU. HI test was performed using commercial Newcastle Disease vaccine (Avinew®) as a source of 4HAU virus antigen. The antibody titre (GMT) levels in 18-26 weeks age group were found to be 70.198, followed by 47.551, 34.776, 17.281 and 18.855 in 27-40, 41-57, 58-73 and >73 weeks age groups, respectively. Moreover, 100% specific immunity against ND was found in 18-26, 27-40 and 41-57 weeks age groups of birds, whereas 93.33 and 94.73% specific immunity was found in 58-73 and >73 weeks age groups, respectively. On an average, 97.87% layer birds showed specific immunity and 2.13% showed nonspecific immunity against NDV. We considered HI titre of 1:8 or above as specific immunity and less than 1:8 as non specific immunity. Highest HI titre was found at the age of 18-26 weeks and lowest titre was at 58-73 weeks of age. The lower level of HI titre seemed to be directly related to some important factors relating to vaccination which have been highlighted in this paper. Key words: Antibody titers; Immune status; HI test; Newcastle disease virus; Layer birds. DOI: http://dx.doi.org/10.3329/ijns.v1i2.8818 International Journal of Natural Sciences (2011), 1(2):35-38

scholarly journals Immune Status Against Newcastle Disease Virus in Backyard Poultry of Punjab Province, Pakistan

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Muhammad Ali Qureshi ◽  
Sami Ullah ◽  
Muhammad Zubair Latif ◽  
Sadia Sarfaraz ◽  
Sohail Ahmed ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Immune Status ◽  
Backyard Poultry ◽  
Punjab Province

scholarly journals Pathogenesis of Velogenic Genotype VII.1.1 Newcastle Disease Virus Isolated from Chicken in Egypt via Different Inoculation Routes: Molecular, Histopathological, and Immunohistochemical Study

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3567
Author(s):  
Yassmin EL-Morshidy ◽  
Walied Abdo ◽  
Ehab Kotb Elmahallawy ◽  
Ghada Allam Abd EL-Dayem ◽  
Ahmed El sawak ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Time Course ◽  
Clinical Signs ◽  
Post Inoculation ◽  
Tissue Samples ◽  
Genetic Characteristics ◽  
Hemagglutination Inhibition Test ◽  
Death Time

Newcastle disease virus (NDV) remains a constant threat to the poultry industry. There is scarce information concerning the pathogenicity and genetic characteristics of the circulating velogenic Newcastle disease virus (NDV) in Egypt. In the present work, NDV was screened from tracheal swabs collected from several broiler chicken farms (N = 12) in Dakahlia Governorate, Egypt. Real-time reverse transcriptase polymerase chain reaction (RRT-PCR) was used for screening of velogenic and mesogenic NDV strains through targeting F gene fragment amplification, followed by sequencing of the resulting PCR products. The identified strain, namely, NDV-CH-EGYPT-F42-DAKAHLIA-2019, was isolated and titrated in the allantoic cavity of 10 day old specific pathogen-free (SPF) embryonated chicken eggs (ECEs), and then their virulence was determined by mean death time (MDT) and intracerebral pathogenicity index (ICPI). The pathogenicity of the identified velogenic NDV strain was also assessed in 28 day old chickens using different inoculation routes as follows: intraocular, choanal slit, intranasal routes, and a combination of both intranasal and intraocular routes. In addition, sera were collected 5 and 10 days post inoculation (pi) for the detection of NDV antibodies by hemagglutination inhibition test (HI), and tissue samples from different organs were collected for histopathological and immunohistochemical examination. A series of different clinical signs and postmortem lesions were recorded with the various routes. Interestingly, histopathology and immunohistochemistry for NDV nucleoprotein displayed widespread systemic distribution. The intensity of viral nucleoprotein immunolabeling was detected within different cells including the epithelial and endothelium lining, as well as macrophages. The onset, distribution, and severity of the observed lesions were remarkably different between various inoculation routes. Collectively, a time-course comparative pathogenesis study of NDV infection demonstrated the role of different routes in the pathogenicity of NDV. The intranasal challenge was associated with a prominent increase in NDV lesions, whereas the choanal slit route was the route least accompanied by severe NDV pathological findings. Clearly, the present findings might be helpful for implementation of proper vaccination strategies against NDV.

scholarly journals Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry

2009 ◽  
Vol 63 (1-2) ◽  
pp. 37-44
Author(s):  
Nenad Milic ◽  
Jakov Nisavic ◽  
Marina Radojicic ◽  
Marina Sekler ◽  
Kazimir Matovic ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Blood Serum ◽  
Disease Virus ◽  
Sensitivity And Specificity ◽  
Newcastle Disease ◽  
Hemagglutination Inhibition ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Hemagglutination Inhibition Test ◽  
Serological Diagnostics

Comparative investigations of the sensitivity and specificity of the indirect immunoenzyme probe - iELISA and the hemagglutination inhibition test (HI test) in serological diagnostics of the Newcastle disease in poultry were carried out using samples of blood serum taken from non-vaccinated and vaccinated poultry. A total of 14 samples of blood serum from non-vaccinated poultry were examined using the immunoenzyme probe - iELISA, and nine of these were found to be positive to the presence of specific antigen against the Newcastle disease virus, while two samples were suspect, and no presence of specific antibodies was established in three samples. Examinations of 82 samples of blood serum from vaccinated poultry for the presence of specific antibodies against the Newcastle disease virus established their presence in 80 serum samples, while one sample was suspect and one sample was negative. The values of the titer of specific antibodies in blood serum samples of vaccinated and non-vaccinated poultry established using the hemagglutination inhibition test (HI test) ranged from 1:2 to 1:32.

Newcastle disease virus and antibody levels in matched sera, ovules and mature eggs of indigenous village hens

2009 ◽  
Vol 32 (1) ◽  
Author(s):  
LW Njagi ◽  
PN Nyaga ◽  
PG Mbuthia ◽  
LC Bebora ◽  
JN Michieka ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Antibody Levels

Titration of Newcastle disease virus and its neutralizing antibodies in microplates by a modified hemadsorption and hemadsorption inhibition method

1976 ◽  
Vol 3 (3) ◽  
pp. 227-232
Author(s):  
Israel Institute for Biological Research, Ness-Ziona, Israel
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Chicken Embryo ◽  
Neutralizing Antibodies ◽  
Inhibition Test ◽  
Virus Infectivity ◽  
Embryo Fibroblasts ◽  
Microneutralization Test ◽  
Chicken Embryo Fibroblasts

Using the microtiter system, titration of Newcastle disease virus infectivity and neutralizing antibodies was carried out in chicken embryo fibroblasts grown in "U" or flat-bottomed plates. Infectivity was detected by a combined hemadsorption-hemagglutination method. Inhibition of that reaction indicated the presence of neutralizing antibodies. A 24-h microneutralization test was developed and compared to the plaque neutralization and microhemagglutination inhibition test. Reproducibility of the microneutralization test was statistically analyzed.

Sign in / Sign up

Export Citation Format

Share Document