Immune Response of Chickens to Six Strains of Newcastle Disease Virus as Measured by Hemagglutination-Inhibition Test

1969 ◽  
Vol 13 (1) ◽  
pp. 46
Author(s):  
P. W. Chang ◽  
V. J. Yates ◽  
J. A. Pendleton ◽  
T. D. Flanagan
Keyword(s):  
Immune Response ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Hemagglutination Inhibition ◽  
Inhibition Test ◽  
Hemagglutination Inhibition Test

scholarly journals Commission testing of the test system “A kit for the detection of antibodies to Newcastle disease virus in hemagglutination inhibition test”

2019 ◽  
pp. 26-31
Author(s):  
B. T. Stegniy ◽  
D. V. Muzyka ◽  
S. V. Tkachenko ◽  
O. M. Rula ◽  
A. B. Stegniy ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Hemagglutination Inhibition ◽  
Test System ◽  
Inhibition Test ◽  
Hemagglutination Inhibition Test ◽  
System A ◽  
Nervous System Function ◽  
Technical Specifications

Newcastle disease or pseudo-plague is a highly contagious viral bird disease, characterized by damage to the respiratory and digestive organs, as well as impaired central nervous system function. Since the middle of the last century, this disease has become widespread in many European countries. Newcastle disease is common in all continents and is especially dangerous. The article provides information on conducting a round of commission tests of the components of the test system “A Kit for Detection of Antibodies to Newcastle Disease Virus in Hemagglutination Inhibition Test”. When testing encrypted antigens and sera with previously characterized and referent samples, they were active and specific, meeting the requirements of the technical specifications of Ukraine for the specified drug. Thus, the positive antigen had activity in the hemagglutination test of 1:256, reacted only with positive to the Newcastle disease virus serum, and did not delay agglutination in the presence of referent to avian influenza virus subtypes H5 and H7 sera. In the presence of positive sera to the Newcastle disease virus in encrypted form, the positive antigen of the corresponding virus delayed the agglutination of the cock erythrocytes in dilutions 1:256–1:512. The results obtained allowed to make a positive decision on the registration of this preparation in the territory of Ukraine

scholarly journals Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry

2009 ◽  
Vol 63 (1-2) ◽  
pp. 37-44
Author(s):  
Nenad Milic ◽  
Jakov Nisavic ◽  
Marina Radojicic ◽  
Marina Sekler ◽  
Kazimir Matovic ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Blood Serum ◽  
Disease Virus ◽  
Sensitivity And Specificity ◽  
Newcastle Disease ◽  
Hemagglutination Inhibition ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Hemagglutination Inhibition Test ◽  
Serological Diagnostics

Comparative investigations of the sensitivity and specificity of the indirect immunoenzyme probe - iELISA and the hemagglutination inhibition test (HI test) in serological diagnostics of the Newcastle disease in poultry were carried out using samples of blood serum taken from non-vaccinated and vaccinated poultry. A total of 14 samples of blood serum from non-vaccinated poultry were examined using the immunoenzyme probe - iELISA, and nine of these were found to be positive to the presence of specific antigen against the Newcastle disease virus, while two samples were suspect, and no presence of specific antibodies was established in three samples. Examinations of 82 samples of blood serum from vaccinated poultry for the presence of specific antibodies against the Newcastle disease virus established their presence in 80 serum samples, while one sample was suspect and one sample was negative. The values of the titer of specific antibodies in blood serum samples of vaccinated and non-vaccinated poultry established using the hemagglutination inhibition test (HI test) ranged from 1:2 to 1:32.

scholarly journals Studies on immune response to Newcastle disease virus in broiler chickens fed with Lactobacillus reuteri PIA16 isolated from the gut of indigenous chicken of Assam, India

2019 ◽  
Vol 12 (8) ◽  
pp. 1251-1255 ◽  
Author(s):  
Gaichamdinliu Gonmei ◽  
Deben Sapcota ◽  
Girin Kumar Saikia ◽  
Pankaj Deka ◽  
Joga Dev Mahanta ◽  
...  
Keyword(s):  
Immune Response ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Hemagglutination Inhibition ◽  
Broiler Chickens ◽  
Lactobacillus Reuteri ◽  
Production Performance ◽  
Indigenous Chicken ◽  
Mannan Oligosaccharide

Background and Aim: The chicken gut harbors microflora which impacts the health, production performance and immune response against pathogens. Assam local chickens reared under natural conditions are known to possess high immunocompetence which may be attributable to its gut microbiota make-up. This study aimed to investigate the individual effect of two strains of Lactobacillus reuteri PIA16 isolated separately from cecum and jejunum of Assam indigenous chicken on the immunity of broiler chickens against Newcastle disease virus (NDV) when fed singly and in combination with a prebiotic. Materials and Methods: A total of 240 birds (48 per group) were vaccinated with Lasota strain of NDV on the 5th and 21st day of age. Blood samples were collected before and after immunization against ND for the detection of humoral antibody response by hemagglutination inhibition test. The cell-mediated immune (CMI) response was estimated through response to phytohemagglutinin-P (PHA-P) and expressed as web index. Results: A significant influence on the immune response to NDV was observed in all the L. reuteri PIA16 as well as mannan oligosaccharide (MOS) supplemented groups revealing higher antibody titer than the control counterpart. The CMI response revealed a better cutaneous basophilic hypersensitivity response to PHA-P in the treated groups than the control. Conclusion: Enhancement in immunity was perceived in the broilers fed with L. reuteri PIA16 and in combination with MOS due to the stimulation of the host's humoral and CMI response by the probiotics and prebiotics used. Keywords: hemagglutination inhibition, Lactobacillus reuteri PIA16, mannan oligosaccharide, Newcastle disease, phytohemagglutinin-P, probiotics.

scholarly journals Optimization of Human Immunodeficiency Virus Gag Expression by Newcastle Disease Virus Vectors for the Induction of Potent Immune Responses

2008 ◽  
Vol 83 (2) ◽  
pp. 584-597 ◽  
Author(s):  
Elena Carnero ◽  
Wenjing Li ◽  
Antonio V. Borderia ◽  
Bruno Moltedo ◽  
Thomas Moran ◽  
...  
Keyword(s):  
Immune Response ◽  
Human Immunodeficiency Virus ◽  
Immune Responses ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Insertion Site ◽  
Gag Protein ◽  
Immunodeficiency Virus

ABSTRACT One attractive strategy for the development of a human immunodeficiency virus (HIV) vaccine is the use of viral vectors with a proven safety profile and an absence of preexisting immunity in humans, such as Newcastle disease virus (NDV). Several NDV vaccine vectors have been generated, and their immunogenicities have been investigated with different animal models. However, a systematic study to evaluate the optimal insertion site of the foreign antigens into NDV that results in enhanced immune responses specific to the antigen has not yet been conducted. In this article, we describe the ability of NDV expressing HIV Gag to generate a Gag-specific immune response in mice. We also have determined the optimal insertion site into the NDV genome by generating recombinant NDV-HIVGag viruses in which HIV gag was located at different transcriptional positions throughout the NDV viral genome. All recombinant viruses were viable, grew to similar titers in embryonated chicken eggs, and expressed Gag in a stable manner. Our in vivo experiments revealed that higher HIV Gag protein expression positively correlates with an enhanced CD8+ T-cell-mediated immune response and protective immunity against challenge with vaccinia virus expressing HIV Gag. We also inserted a codon-optimized version of HIV gag in the described best location, between the P and M genes. Virus expressing the codon-optimized version of HIV gag induced a higher expression of the protein and an enhanced immune response against HIV Gag in mice. These results indicate that strategies directed toward increasing antigen expression by NDV result in enhanced immunogenicity and vaccine efficacy.

scholarly journals Effect of Immunosuppression on Newcastle Disease Virus Persistence in Ducks with Different Immune Status

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Lucy W. Njagi ◽  
Phillip N. Nyaga ◽  
Lilly C. Bebora ◽  
Paul G. Mbuthia ◽  
Uswege M. Minga
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Immune Status ◽  
Experimental Period ◽  
Hemagglutination Inhibition Test ◽  
Viral Excretion ◽  
Antibody Titres ◽  
Antibody Levels ◽  
Chicken Embryo Fibroblasts

This study was carried out to verify the possibility that ducks are sources of Newcastle disease (ND) virus infection for chickens in mixed flocks. Immunosuppressed (IS) and non immunosuppressed (NIS) birds, at three different antibody levels (medium, low and absent) were used; the titres having been induced through vaccination, and Immunosuppression done using dexamethazone. Each of the 3 respective groups was further divided into 2 groups of about 12 ducks each: one challenged with velogenic ND virus; the other not challenged. Selected ducks from all groups had their antibody titres monitored serially using hemagglutination inhibition test, while two birds from each of the challenged groups were killed and respective tissues processed for ND viral recovery, using chicken embryo fibroblasts. In general, antibody titres of IS and NIS challenged ducks were significantly higher than their unchallenged counterparts (P<0.05). Non-challenged pre-immunised ducks had a progressive decrease in antibody levels; non-immunised ducks did not seroconvert. Newcastle disease virus was isolated from livers and kidneys of the challenged ducks throughout the experimental period; indicating a possibility of viral excretion, especially when the birds are stressed. It, therefore, provides another possible model of viral circulation within mixed flocks.

scholarly journals Comparative safety and efficacy of two bivalent vaccines containing Newcastle disease LaSota and avian influenza H9N2 Sidrap isolate formulated with different oil adjuvants

2020 ◽  
Vol 13 (11) ◽  
pp. 2493-2501
Author(s):  
Jossie Intan Cahyani ◽  
Sitarina Widyarini ◽  
Michael Haryadi Wibowo
Keyword(s):  
Immune Response ◽  
Avian Influenza ◽  
Newcastle Disease ◽  
Hemagglutination Inhibition ◽  
Mineral Oil ◽  
Economic Losses ◽  
Poultry Industry ◽  
Bivalent Vaccine ◽  
Hemagglutination Inhibition Test ◽  
White Mineral

Background and Aim: Newcastle disease (ND) and avian influenza (AI) are two devastating diseases of poultry, which cause great economic losses to the poultry industry and disrupt food security in our country. The use of ND-AI inactive bivalent vaccine is very effective and economical to prevent and control ND and AI disease. Bivalent ND LaSota-AI H9N2 vaccine is not yet available in Indonesia. The inactivated vaccines used in poultry industry often require oil adjuvant to elicit a sufficient immune response. This study aimed to develop the bivalent inactive vaccines containing ND LaSota and AI H9N2 Sidrap isolate which are local isolates as poultry vaccine candidates, and formulated with two different commercial adjuvants, then compared. Materials and Methods: Two vaccines bivalent were prepared by emulsifying inactivated Newcastle disease virus (LaSota strain) and AI H9N2 Sidrap isolate viruses with Marcol white mineral oil and Montanide ISA70 adjuvants. Both of bivalent vaccines were tested for safety (physical and histopathological at the injection site) and efficacy in specific-pathogen-free chickens. Parameters used for the evaluation of the efficacy were immunogenicity by hemagglutination inhibition and protection percentage. Results: Both bivalent vaccines are safe to use. Post-vaccination (PV) immune response was observed using a hemagglutination inhibition test at 2, 3, 4, 5, 6, 7, and 8 weeks of PV. The bivalent vaccine B gives a better immune response to ND at 2, 3, and 4 weeks of PV (p<0.05) compared to the bivalent vaccine A, but in 5, 6, 7, and 8 weeks, the PV does not show differences in the immune response. The immune response to AI H9N2 showed differences at weeks 2 and 3 PV (p<0.05) with the bivalent vaccine B indicated higher immunity. A single immunization with both bivalent vaccines induces 100% protection in chickens that have been vaccinated against the deadly challenge with the virulent ND virus. Conclusion: Both of bivalent vaccines are safe to use and provide good efficacy against virulent ND viruses, but bivalent vaccine B (with Montanide ISA70 adjuvant) shows better immune response than bivalent vaccine A (Marcol white mineral oil adjuvant).

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