scholarly journals Germination of Primed Seed under NaCl Stress in Wheat

ISRN Botany ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Michael P. Fuller ◽  
Jalal H. Hamza ◽  
Hail Z. Rihan ◽  
Mohammad Al-Issawi

Soil salinity affects a large and increasing amount of arable land worldwide, and genetic and agronomic solutions to increasing salt tolerance are urgently needed. Experiments were conducted to improve wheat seed performance under salinity stress conditions after priming. An experiment was conducted using a completely randomized design of four replications for germination indices in wheat (Triticum aestivum L. cv. Caxton). Normal and primed seed with PEG6000 at  MPa and five concentrations of NaCl (0, 50, 100, 150, and 200 mM) were tested. Results indicate that priming seed significantly () increased germination percentage at first count and final count, coefficient of velocity of germination, germination rate index, and mean germination time, while increasing of NaCl concentration significantly reduced it. Priming seed improved germination attributes at all NaCl concentration levels. The priming appeared to be able to overcome the effect of salt stress at 50 to 100 mM and reduce the effect of NaCl at higher concentrations up to 200 mM. The primed seed gave both faster germination and led to higher germination when under salt stress. We conclude that using priming techniques can effectively enhance the germination seed under saline condition.

2017 ◽  
Vol 6 (06) ◽  
pp. 1634
Author(s):  
Ebeny Leonny Tsague ◽  
Eric Bertrand Kouam* ◽  
Christopher Mubeteneh Tankou

This study investigates the effect of salt (NaCl) on the germination of seeds of 10 genotypes of cowpea (Vigna unguiculata). The experiment carried out on petri dishes was done using a completely randomized design comprising three replications. The germination of the cultivars was studied using distilled water as control and under osmotic potentials of 50, 100, 150 and 200mM NaCl. The results indicated that the Mean Germination Time (MGT), Time to 50% Germination (T50), Germination Index (GI), Coefficient of Velocity of Germination (CVG), and Germination Percentage (GP), varied between 2.33 and 5.51 days; 2.83 and 14.87 days; 16.66 and 1.63; 0.53 and 0.11; 87.11 and 27.98% respectively. The variables GI, CVG and GP tended to decrease with increasing NaCl concentration while MGT and T50 tended to increase with increasing salt concentrations. For all the germination variables, significant differences at 0.001 probability levels were found among salinity treatments, cowpea genotypes and their interaction. Four genotypes (KEB-CP118, OU59A, OU100 and ICV12) were tolerant to salt stress and performed well under salinity conditions. Four other genotypes were found to be moderately tolerant (ET11, NO1036, MTA22 and KEB-CP009) and two genotypes (KEB-CP098 and NO193) were susceptible to salt stress and were most affected by salinity. At 0.001 probability level, positive correlations were observed between GP and GI (r = 0.792); GP and CVG (r = 0.774); GI and CVG (r = 0.955) and negative correlations were observed between GP and T50 (r = - 0.845); GI and MGT (r = - 0.894); CVG and MGT (r = - 0.895).


2017 ◽  
Vol 9 (2) ◽  
pp. 242-249 ◽  
Author(s):  
Faride BEHBOUDI ◽  
Zeinalabedin TAHMASEBI SARVESTANI ◽  
Mohamad Zaman KASSAEE ◽  
Seyed Ali Mohamad MODARES SANAVI ◽  
Ali SOROOSHZADEH

Plants such as wheat and barley that are strategically important crops need to be considered to develop a comprehensive toxicity profile for nanoparticles (NPs). The present study was aimed to investigate the effects of chitosan and SiO2 NPs on wheat and barley plants. Two factorial experiments (seeds priming and direct exposure) were performed based on a completely randomized design in four replications. Results showed that the seeds priming with the NPs had not significant effect on germination parameters such as Germination Percentage (GP), Germination Rate (GR), Germination Value (GV), Mean Germination Time (MGT), Pick Value (PV) and Mean Daily Germination (MDG). In contrast, exposure of the seeds to the NPs had significant effects on these parameters. In both experiments, treatments had significant effects on shoot, seedling, root length, fresh and dry weight, as well as vigor indexes as compared to the control. In most traits, the best concentration of NPs was 30 ppm, whereas applications of the NPs with 90 ppm displayed adverse effects on majority of the studied traits. According to these results, selectivity in applications of NPs with suitable concentration and method is essential for different plant species.  


Author(s):  
Panayiota PAPASTYLIANOU ◽  
Ilias TRAVLOS ◽  
Ioannis ROUSSIS ◽  
Dimitrios BILALIS

Teff is an annual grass originated in Ethiopia, grown for both human food and animal feed. Salinity and moisture stress are the main limiting factors of agricultural development on arid and semi-arid regions. The aim of this study was to investigate the effect of salinity due to NaCl on germination of teff. A set of experiments was conducted under a completely randomized design with four replications of 200 seeds at two different suboptimal germination temperatures (15 and 25 °C) in the dark for 10 days. In order to create salt stress conditions during the germination process, seven solutions were used (0 as control, 80, 160, 240 and 320, 400 and 480 mM NaCl). The germination performance was evaluated by final germination percentage and mean germination time. The presence of NaCl reduced germination, especially above 240 mM for 15 °C and 400 mM for 25 °C. The mean germination time increased with higher NaCl levels.


2015 ◽  
Vol 9 (1) ◽  
pp. 6-11
Author(s):  
Abolfazl Masoumi Zavariyan ◽  
Mojtaba Yousefi Rad ◽  
Mohsen Asghari

For investigation of the effect of seed priming by kinetin on germination indices and proline activity of Silybum marianum  L. under drought stress, factorial experiment based on completely randomized design with two factor includingdifferent concentrations of kinetin (0, 10 and 20 ppm) and different levels of drought stress including 0, -6 and -12 barat three replicates was carried out. According to the results, drought stress and kinetin individualy cause to decreases of germination percentage, germination rate, seedling length, seed vigor and seedling dry weight but uses of kinetin in drought stress condition improvment of them. Also, drought stress cause to increases of proline content and catalase activity. Therefore, seed priming with 10 ppm kinetin at drought stress was improved the germination indices of Silybum marianum L.DOI: http://dx.doi.org/10.3126/ijls.v9i1.11919 International Journal of Life Sciences Vol.9(1) 2015 6-11


2012 ◽  
Vol 47 (8) ◽  
pp. 1181-1184 ◽  
Author(s):  
Badar‑uz‑Zaman ◽  
Arshad Ali ◽  
Syed Ishtiaq Hyder ◽  
Muhammad Arshadullah ◽  
Saqib Umar Bhatti

The objective of this work was to determine if KCl could be a useful nutrient primer for safe seed germination in maize crop under salt stress conditions. Seed priming was done using 50 mmol L‑1 of muriate of potash, and germination and seedling growth were evaluated after salt stress with NaCl up to 50 mmol L‑1. Another set of seeds was tested under the same salt stress conditions without priming. Under salinity stress, germination percentage, germination rate index, germination coefficient, and seedling vigor indexes were higher in primed seeds. In unprimed seeds, mean germination time increased, while the germination rate index and the fresh and dry matter mass decreased more sharply with salinity stress. The Na/K ratio was higher in unprimed seeds.


2018 ◽  
Vol 36 (0) ◽  
Author(s):  
J.M.L. AZEVEDO ◽  
C.G.G. SANTOS ◽  
C.S. CAIRES ◽  
J.C. ARAÚJO NETO ◽  
R.C. SOUZA

ABSTRACT: The goal of this work was to characterize morphometrically diaspores from three species of mistletoes (Passovia pyrifolia, Struthanthus marginatus e Phoradendron mucronatum), as well as to study its germination behavior according to temperature and luminosity. In germination tests, the completely randomized design was used, in factor scheme 4 x 2, with 4 replications of 15 diaspores. The factors for the first test were different temperatures and two diaspore conditions (intact and without epicarp). In the second test, factors were different light qualities and two diaspore conditions. The analyzed variables were: germination percentage, germination rate index (GRI) and mean germination time (MGT). Mistletoe’s diaspores present an elliptic shape with viscin layer, an essential characteristic for the dispersion of the species. In addition to this, they present a distinction as for the moisture content when compared to the Loranthaceae and Viscaceae, where the latter presents the highest content. Germination is greater at the alternated temperature of 20-30 oC for P. pyrifolia and P. mucronatum diaspores and 20 oC for S. marginatus. Luminosity interfered in the germination of P. mucronatum and S. marginatus but did not influence P. pyrifolia. The diaspore condition influenced the germination of the three studied species, observing a significant increase in their germination when the epicarp was removed. Temperature and luminosity interfere directly in the GRI and MGT of the three mistletoes species.


2021 ◽  
pp. 76-82
Author(s):  
Getachew Shumye Adilu ◽  
Yohannes Gedamu Gebre

Salinity adversely affects 20-30% of the irrigated area in the world. Tomato is sensitive to salinity. It is one of the most severe abiotic factors of many agricultural crops and it becoming the main problem in Ethiopia. This study was conducted to evaluate the effects of different salinity levels on the seed germination parameters of tomato varieties. It was laid out in a completely randomized design with three replicates. The treatment included four tomato varieties (Sirinka, Weyno, ARP D2, and Roma VF) and five salinity levels (1 dS m-1, 2 dS m-1, 3 dS m-1, 4 dS m-1, and control). Fifty seeds were placed in a Petri dish over a moistened germination paper for germination and seedlings and allowed to grow for 14 days. The germination rate, speed and energy of tomato seeds were significantly (p < 0.001) affected by the combined effect of variety and salinity. The shortest mean germination time, the highest mean germination rate, and the highest speed of germination were recorded in the ARP D2 variety in the control treatment. The lowest first and last days of germination, and the uncertainty of germination were recorded from ARP D2. However, an increase in the days of germination and in the uncertainty of germination, and a decrease in the germination index and total germination percentage trends were observed with increasing salinity levels. The highest level of salinity (4 dS m-1) affected the germination of tomato varieties. Among the four tested tomato varieties, ARP D2 and Roma VF were tolerant to salinity.


2014 ◽  
Vol 6 (3) ◽  
pp. 374-380
Author(s):  
Yousef NASIRI ◽  
Parisa FEYZI ◽  
Abdollah JAVANMARD

Salinity is an abiotic stress which has harmful effects on germination of many plants. Therefore, high germination rate and vigorous early growth under salty soils is preferred. Seed priming is a way to increase salt tolerance of plants. An experiment was conducted to investigate the effect of seed priming on germination of milk thistle under salinity condition. The treatments were 4 levels of seed priming (no priming, distilled water as hydro priming and 0.5 and 1.0 mM salicylic acid) and 5 levels of salinity (0, 40 and 80 mM NaCl and 40 and 80 mM CaCl2). The experiment arranged as a factorial in a completely randomized design (CRD) with three replications. Results showed that salinity decreased germination percentage and germination rate to about 16 and 32% in 80 mM CaCl2 level compared to control, respectively. The highest mean germination time (5.7 day) were belonged to 80 mM CaCl2. Radicle and plumule length significantly decreased by 80 mM NaCl and 40 and 80 mM CaCl2. The lowest seedling weight and seed stamina observed in 80 mM CaCl2. 0.5 mM salicylic acid improved all traits except mean germination time as compared to control.  Salicylic acid (0.5 mM) improved radicle length under 0, 40 and 80 mM NaCl salinity levels as well as increased plumule length at the 0 and 40 mM NaCl salinity conditions.


2015 ◽  
Vol 61 (1) ◽  
pp. 5-18 ◽  
Author(s):  
Majid Dashti ◽  
Mohammad Kafi ◽  
Hossein Tavakkoli ◽  
Mahdi Mirza

Summary The focus of this study is based on the examination of the germination traits and the development of thermal models of the medicinal plant Salvia leriifolia Benth. A laboratory experiment was carried out at constant temperatures ranging from 0 to 35°C, at 5°C intervals in a completely randomized design with eight replications. To describe the germination rate response to temperature, three regression models, namely Intersected-Lines (ISL), Quadratic Polynomial (QPN) and Five-Parameters Beta (FPB) were used. The highest Germination Percentage (GP) (92.8%) occurred in 15°C, but GP in the range of 10-25°C was not significant (p≤0.05). The germination process stopped at 0°C and at above 30°C. The results indicated that the highest Germination Rate (GR), the lowest Mean Germination Time (MGT) and also times to 50% germination (D50) were obtained at 20°C. Seeds did not reach to their 50% germination level in temperatures higher than 25°C. The FPB model had the best realistic estimation for cardinal temperatures. Based on models estimation, Base (Tb), Optimum (To) and Ceiling (Tc) temperatures were in the ranges of (1-1.9°C), (18.1-20.8°C) and (34.5-38.7°C), respectively.


2014 ◽  
Vol 67 (1) ◽  
pp. 115-123 ◽  
Author(s):  
H. Saeb ◽  
M. Khayyat ◽  
A. Zarezadeh ◽  
F. Moradinezhad ◽  
A. Samadzadeh ◽  
...  

Abstract The present research was conducted to evaluate the effects of salinized water with NaCl on seed germination of Periwinkle and Corn Poppy. Treatments were: A) H2O- distilled water (control); B) sodium chloride solutions (5, 10, 20, 40 and 80 mM). Application of the highest NaCl concentration (80mM) significantly reduced germination percentage and mean germination time of both species, although increased the day of 50% germination. Increment of salinity concentration was led to the reduction of radicle length in Periwinkle. The seedling fresh weight and water content and radicle length of Corn Poppy were decreased in both levels of 20 and 80 mM, and seedling dry weight was unaffected by treatments. It is concluded that both species are tolerant to NaCl salinity up to 80 mM during germination stage.


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