scholarly journals Salinity tolerance at germination of some main cultivated cowpea (Vigna unguiculata) genotypes from Western Cameroon

2017 ◽  
Vol 6 (06) ◽  
pp. 1634
Author(s):  
Ebeny Leonny Tsague ◽  
Eric Bertrand Kouam* ◽  
Christopher Mubeteneh Tankou
Keyword(s):  
Salt Stress ◽  
Vigna Unguiculata ◽  
Germination Percentage ◽  
Randomized Design ◽  
Nacl Concentration ◽  
Mean Germination Time ◽  
The Mean ◽  
Coefficient Of Velocity ◽  
Positive Correlations ◽  
Osmotic Potentials

This study investigates the effect of salt (NaCl) on the germination of seeds of 10 genotypes of cowpea (Vigna unguiculata). The experiment carried out on petri dishes was done using a completely randomized design comprising three replications. The germination of the cultivars was studied using distilled water as control and under osmotic potentials of 50, 100, 150 and 200mM NaCl. The results indicated that the Mean Germination Time (MGT), Time to 50% Germination (T50), Germination Index (GI), Coefficient of Velocity of Germination (CVG), and Germination Percentage (GP), varied between 2.33 and 5.51 days; 2.83 and 14.87 days; 16.66 and 1.63; 0.53 and 0.11; 87.11 and 27.98% respectively. The variables GI, CVG and GP tended to decrease with increasing NaCl concentration while MGT and T50 tended to increase with increasing salt concentrations. For all the germination variables, significant differences at 0.001 probability levels were found among salinity treatments, cowpea genotypes and their interaction. Four genotypes (KEB-CP118, OU59A, OU100 and ICV12) were tolerant to salt stress and performed well under salinity conditions. Four other genotypes were found to be moderately tolerant (ET11, NO1036, MTA22 and KEB-CP009) and two genotypes (KEB-CP098 and NO193) were susceptible to salt stress and were most affected by salinity. At 0.001 probability level, positive correlations were observed between GP and GI (r = 0.792); GP and CVG (r = 0.774); GI and CVG (r = 0.955) and negative correlations were observed between GP and T50 (r = - 0.845); GI and MGT (r = - 0.894); CVG and MGT (r = - 0.895).

scholarly journals Germination of Primed Seed under NaCl Stress in Wheat

ISRN Botany ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Michael P. Fuller ◽  
Jalal H. Hamza ◽  
Hail Z. Rihan ◽  
Mohammad Al-Issawi
Keyword(s):  
Salt Stress ◽  
Germination Rate ◽  
Arable Land ◽  
Nacl Stress ◽  
Germination Percentage ◽  
Randomized Design ◽  
Nacl Concentration ◽  
Mean Germination Time ◽  
Coefficient Of Velocity ◽  
Germination Indices

Soil salinity affects a large and increasing amount of arable land worldwide, and genetic and agronomic solutions to increasing salt tolerance are urgently needed. Experiments were conducted to improve wheat seed performance under salinity stress conditions after priming. An experiment was conducted using a completely randomized design of four replications for germination indices in wheat (Triticum aestivum L. cv. Caxton). Normal and primed seed with PEG6000 at  MPa and five concentrations of NaCl (0, 50, 100, 150, and 200 mM) were tested. Results indicate that priming seed significantly () increased germination percentage at first count and final count, coefficient of velocity of germination, germination rate index, and mean germination time, while increasing of NaCl concentration significantly reduced it. Priming seed improved germination attributes at all NaCl concentration levels. The priming appeared to be able to overcome the effect of salt stress at 50 to 100 mM and reduce the effect of NaCl at higher concentrations up to 200 mM. The primed seed gave both faster germination and led to higher germination when under salt stress. We conclude that using priming techniques can effectively enhance the germination seed under saline condition.

scholarly journals Sensitivity of Seed Germination to Salt Stress in Teff [Eragrostis tef (Zucc.) Trotter]

2019 ◽  
Vol 76 (1) ◽  
pp. 91
Author(s):  
Panayiota PAPASTYLIANOU ◽  
Ilias TRAVLOS ◽  
Ioannis ROUSSIS ◽  
Dimitrios BILALIS
Keyword(s):  
Salt Stress ◽  
Agricultural Development ◽  
Animal Feed ◽  
Moisture Stress ◽  
Germination Percentage ◽  
Limiting Factors ◽  
Eragrostis Tef ◽  
Germination Time ◽  
Randomized Design ◽  
Mean Germination Time

Teff is an annual grass originated in Ethiopia, grown for both human food and animal feed. Salinity and moisture stress are the main limiting factors of agricultural development on arid and semi-arid regions. The aim of this study was to investigate the effect of salinity due to NaCl on germination of teff. A set of experiments was conducted under a completely randomized design with four replications of 200 seeds at two different suboptimal germination temperatures (15 and 25 °C) in the dark for 10 days. In order to create salt stress conditions during the germination process, seven solutions were used (0 as control, 80, 160, 240 and 320, 400 and 480 mM NaCl). The germination performance was evaluated by final germination percentage and mean germination time. The presence of NaCl reduced germination, especially above 240 mM for 15 °C and 400 mM for 25 °C. The mean germination time increased with higher NaCl levels.

scholarly journals RESPOSTA GERMINATIVA DO FEIJÃO ARIGÓ E MANTEIGUINHA (Vinga unguicula L. Walp) SUBMETIDAS A CONDIÇÕES ESTRESSE SALINO

Nativa ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 165
Author(s):  
Kelly Nascimento Leite ◽  
Yasmin Cavalcante de Andrade ◽  
Kecy Dhones Monteiro Marques ◽  
André Luiz Melhorança Filho ◽  
Porfirio Ponciano de Oliveira Júnior ◽  
...  
Keyword(s):  
Vigna Unguiculata ◽  
Dry Matter ◽  
Good Alternative ◽  
Hypocotyl Growth ◽  
Total Period ◽  
Good Tolerance ◽  
Germination Time ◽  
Randomized Design ◽  
Mean Germination Time ◽  
The Mean

Esse trabalho teve o objetivo de avaliar a germinação e crescimento de plântula das sementes crioulas de feijão-caupi, (Vigna unguiculata (L.) Walp), variedades Manteguinha e Arigó, exposta a níveis crescentes de salinidade da região do vale do Juruá, Acre. O experimento foi conduzido em laboratório utilizando 10 tratamentos e quatro repetições distribuídos seguindo um delineamento inteiramente casualizado em esquema fatorial 2 x 5, duas variedades de feijão (Arigó e Manteguinha) e 5 níveis de salinidade, por um período total de 7 dias. Foi avaliado o índice de germinação, o tempo médio de germinação, crescimento do hipocótilo, crescimento da radícula, e matéria seca total. Os resultados mostraram que a germinação (IVG), hipocótilo (CH) e raiz (CR) são afetados com o aumento das concentrações salinas. O tempo médio de germinação para a variedade Arigó não sofreu alteração significativa com a presença dos sais na solução. A matéria seca do hipocótilo e raiz foram afetadas de maneira significativa somente para a variedade Arigó. A variedade Manteguinha apresentou boa tolerância à salinidade na germinação inicial, mostrando-se como uma boa alternativa para programas de seleção de obtenção de cultivares tolerantes ao estresse salino.Palavras-chave: feijão-caupi; salinidade; germinação. GERMINATION RESPONSE OF BEAN ARIGÓ AND MANTEGUINHA (Vigna unguiculata (L.) Walp) SUBMITTED TO SALT STRESS CONDITIONS ABSTRACT: The objective of this work was to evaluate the germination and seedling growth of the Creole seeds of the Juruá Valley, Acre, bean caupi (Vigna unguiculata (L.) Walp) varieties Manteguinha and Arigó, exposed to increasing levels of salinity. The experiment was conducted in a laboratory using 10 treatments and four replicates distributed in a completely randomized design in a 2 x 5 factorial scheme, two varieties of bean (Arigó and Manteguinha) and 5 levels of salinity, for a total period of 7 days. The germination index, mean germination time, hypocotyl growth, root growth, and total dry matter were evaluated. The results showed that germination (IVG), hypocotyl (CH) and root (CR) are affected with the increased salt concentrations. The mean germination time for the Arigó variety did not change significantly with the presence of the salts in the solution. The dry matter of the hypocotyl and root is affected only significantly for the arthropod variety. The variety Manteguinha presented good tolerance to the salinity in the initial germination. The butterbean variety stood out best by showing a good alternative for selection programs for obtaining salt tolerant cultivars.Keywords: cowpea beans; salinity; germination.

scholarly journals Sensitivity of Seed Germination to Salt Stress in Black Cumin (Nigella sativa L.)

2018 ◽  
Vol 46 (1) ◽  
pp. 202-205 ◽  
Author(s):  
Panayiota PAPASTYLIANOU ◽  
Nikolia-Nonika BAKOGIANNI ◽  
Ilias TRAVLOS ◽  
Ioannis ROUSSIS
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Nigella Sativa ◽  
Germination Percentage ◽  
Oilseed Crop ◽  
Black Cumin ◽  
Germination Time ◽  
Randomized Design ◽  
Mean Germination Time ◽  
Major Factors

Black cumin is an annual oilseed crop, native to the Mediterranean region and widely used for nutritional and medicinal purposes. Reduced seed germination and early seedling growth under saline conditions are considered as major factors limiting the establishment of crops. The objective of this study was to investigate the effect of salinity due to NaCl on germination of black cumin seeds. A set of experiments was conducted under completely randomized design with four replications of 200 seeds at two different suboptimal germination temperatures (15 and 20 °C) in the dark for 16 days. In order to create salt stress conditions during the germination process, five solutions were used (0 as control, 80, 160, 240 and 320 mM NaCl). The germination performance was evaluated by final germination percentage and mean germination time. The effect of salinity on seed germination percentage and mean germination time was significant at p<0.01. Germination of the controls was 96% and 95% at 15 °C and 20 °C, respectively. The presence of NaCl, regardless of temperature, reduced germination. This reduction was more severe at 15 °C (15%, and 19% germination in cases of 80 and 160 mM concentration respectively) than at 20 °C (65% at 80 mM salinity level) and no germination was observed from 240 mM onwards. The mean germination time increased with increased NaCl levels, especially at the lower temperature of 15 °C. The overall results of this experiment showed the inhibitory effects of salt stress on seed germination parameters of black cumin.

scholarly journals GERMINATION AND VIGOUR IN SEEDS OF THE COWPEA IN RESPONSE TO SALT AND HEAT STRESS

2019 ◽  
Vol 32 (1) ◽  
pp. 143-151 ◽  
Author(s):  
Luma Rayane de Lima Nunes ◽  
Paloma Rayane Pinheiro ◽  
Charles Lobo Pinheiro ◽  
Kelly Andressa Peres Lima ◽  
Alek Sandro Dutra
Keyword(s):  
Salt Stress ◽  
Heat Stress ◽  
High Temperature ◽  
Low Temperature ◽  
Vigna Unguiculata ◽  
Salt Concentration ◽  
Dry Weight ◽  
Germination Percentage ◽  
Different Types ◽  
Different Temperatures

ABSTRACT Salinity is prejudicial to plant development, causing different types of damage to species, or even between genotypes of the same species, with the effects being aggravated when combined with other types of stress, such as heat stress. The aim of this study was to evaluate the tolerance of cowpea genotypes (Vigna unguiculata L. Walp.) to salt stress at different temperatures. Seeds of the Pujante, Epace 10 and Marataoã genotypes were placed on paper rolls (Germitest®) moistened with different salt concentrations of 0.0 (control), 1.5, 3.0, 4.5 and 6.0 dS m-1, and placed in a germination chamber (BOD) at temperatures of 20, 25, 30 and 35°C. The experiment was conducted in a completely randomised design, in a 3 × 4 × 5 scheme of subdivided plots, with four replications per treatment. The variables under analysis were germination percentage, first germination count, shoot and root length, and total seedling dry weight. At temperatures of 30 and 35°C, increases in the salt concentration were more damaging to germination in the Epace 10 and Pujante genotypes, while for the Marataoã genotype, damage occurred at the temperature of 20°C. At 25°C, germination and vigour in the genotypes were higher, with the Pujante genotype proving to be more tolerant to salt stress, whereas Epace 10 and Marataoã were more tolerant to high temperatures. Germination in the cowpea genotypes was more sensitive to salt stress when subjected to heat stress caused by the low temperature of 20°C or high temperature of 35°C.

scholarly journals Phytotoxicity of Chitosan and SiO2 Nanoparticles to Seed Germination of Wheat (Triticum aestivum L.) and Barley (Hordeum vulgare L.) Plants

2017 ◽  
Vol 9 (2) ◽  
pp. 242-249 ◽  
Author(s):  
Faride BEHBOUDI ◽  
Zeinalabedin TAHMASEBI SARVESTANI ◽  
Mohamad Zaman KASSAEE ◽  
Seyed Ali Mohamad MODARES SANAVI ◽  
Ali SOROOSHZADEH
Keyword(s):  
Germination Rate ◽  
Dry Weight ◽  
Sio2 Nanoparticles ◽  
Triticum Aestivum L ◽  
Germination Percentage ◽  
Hordeum Vulgare L ◽  
Randomized Design ◽  
Direct Exposure ◽  
Contrast Exposure ◽  
Mean Germination Time

Plants such as wheat and barley that are strategically important crops need to be considered to develop a comprehensive toxicity profile for nanoparticles (NPs). The present study was aimed to investigate the effects of chitosan and SiO2 NPs on wheat and barley plants. Two factorial experiments (seeds priming and direct exposure) were performed based on a completely randomized design in four replications. Results showed that the seeds priming with the NPs had not significant effect on germination parameters such as Germination Percentage (GP), Germination Rate (GR), Germination Value (GV), Mean Germination Time (MGT), Pick Value (PV) and Mean Daily Germination (MDG). In contrast, exposure of the seeds to the NPs had significant effects on these parameters. In both experiments, treatments had significant effects on shoot, seedling, root length, fresh and dry weight, as well as vigor indexes as compared to the control. In most traits, the best concentration of NPs was 30 ppm, whereas applications of the NPs with 90 ppm displayed adverse effects on majority of the studied traits. According to these results, selectivity in applications of NPs with suitable concentration and method is essential for different plant species.  

scholarly journals Temperatures and substrates for germination and vigor of Erythroxylum pauferrense Plowman seeds

2019 ◽  
Vol 41 ◽  
pp. e46030
Author(s):  
João Everthon da Silva Ribeiro ◽  
Arliston Pereira Leite ◽  
Jackson Silva Nóbrega ◽  
Edna Ursulino Alves ◽  
Riselane de Lucena Alcântara Bruno ◽  
...  
Keyword(s):  
Dry Mass ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Randomized Design ◽  
Temperature Regimes ◽  
Seedling Length ◽  
Physiological Quality ◽  
Mean Germination Time ◽  
Completely Randomized Design ◽  
Understory Plant

Erythroxylum pauferrense is an endemic understory plant species of the Northeast Region of Brazil. The species is of great importance to the region and so ecophysiological studies are needed for its preservation. The objective of the present study was to determine the best substrates and temperatures for testing germination and seed vigor of E. pauferrense. An experiment was performed comprising a completely randomized design in a 5 x 4 factorial scheme, with five temperature regimes (20, 25, 30, 35°C constant and 20-30°C alternating) and four types of substrates (paper, vermiculite, sand and commercial substrate). The following characteristics were evaluated: germination percentage, first germination count, germination speed index, mean germination time, seedling length and dry mass (root and shoot). Paper and vermiculite substrates combined with constant temperatures of 20, 25, 30 and alternated between 20-30°C, provide greater seed germination and vigor while 35°C reduces seed physiological quality.

scholarly journals Physiological quality of osmoprimed gherkin seeds

2013 ◽  
Vol 35 (3) ◽  
pp. 368-373 ◽  
Author(s):  
Rodrigo de Góes Esperon Reis ◽  
Humberto Pereira da Silva ◽  
José Maria Gomes Neves ◽  
Renato Mendes Guimarães
Keyword(s):  
Electrical Conductivity ◽  
Room Temperature ◽  
Dry Weight ◽  
Potassium Nitrate ◽  
Germination Percentage ◽  
Initial Profile ◽  
Randomized Design ◽  
Physiological Quality ◽  
Osmotic Potentials

It was aimed to verify the effect of different methodologies of osmopriming on physiological quality of gherkin seeds. Prior, it has been characterized the initial profile of the gherkin seeds. Then, the seeds were osmoprimed in gerboxes containing two blotter papers wetted with osmotic solutions in a volume equal to three times the paper dry weight and kept in BOD at 15 °C. After the priming, the seeds were dried at room temperature for 48 hours. Then, the following variables were evaluated: seeds moisture content, percentage of germination, percentage of emergence, emergence speed index and electrical conductivity. The analyses of variances were realized according to a randomized design in a factorial scheme 3 x 2 x 4: three solutes (polyetilene glycol 6000 - PEG, potassium nitrate - KNO3 and PEG + KNO3), two osmotic potentials (-0.55 and -1.10 MPa) and four times of priming (24, 48, 72 and 96 hours), followed by analyses of regression. It is concluded that the priming has no effect on germination and affects positively the vigor of the gherkin seeds lots; osmopriming with potassium nitrate is effective in improving the physiological quality of gherkin seeds lot.

scholarly journals Sensitivity of Seed Germination to Temperature and Light in Helichrysum amorginum

2020 ◽  
Vol 77 (1) ◽  
pp. 7
Author(s):  
Sofia ARGYRIOU ◽  
Panayiota PAPASTYLIANOU ◽  
George STAVROPOULOS ◽  
Angeliki KOUSTA ◽  
Ioanna KAKABOUKI ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seedling Survival ◽  
Germination Percentage ◽  
Effect Of Temperature ◽  
Germination Time ◽  
Randomized Design ◽  
Perennial Shrub ◽  
Mean Germination Time ◽  
Lower Temperature ◽  
Seed Propagation

Helichrysum amorginum is a perennial shrub, endemic on the Greek island of Amorgos and used for medicinal, cosmetic and ornamental purposes. Information concerning germination behavior and seedling survival is considered essential for the establishment and cultivation of wild species. The objective of this study was to investigate the effect of temperature and light on germination of Helichrysum amorginum seeds. A set of experiments was conducted under completely randomized design with four replications of 200 seeds at three different suboptimal germination temperatures (15, 20 and 25°C) in light and dark conditions for 10 days. Seed material was collected from two different fields of the island (Aegiali and Katapola). The germination performance was evaluated by final germination percentage and mean germination time. The effect of temperature on seed germination percentage and mean germination time was significant in all cases. The presence of light enhanced germination, especially at the optimum temperature of 20°C. In most cases the mean germination time increased at the lower temperature of 15°C. Seeds from Aegiali exhibited higher germination ability than the seed lot from Katapola. Knowledge of seed germination behaviour is of major importance in order to establish effective protocols for seed propagation in Helichrysum amorginum.

scholarly journals GERMINATION OF CREOLE MAIZE AND FAVA BEAN SEEDS UNDER SALT STRESS

2020 ◽  
Vol 33 (3) ◽  
pp. 853-859
Author(s):  
CARLA DE SOUZA ALMEIDA ◽  
HUGO ROLDI GUARIZ ◽  
MARÍLIA ALVES BRITO PINTO ◽  
MARINEIDE FERREIRA DE ALMEIDA
Keyword(s):  
Salt Stress ◽  
Rural Areas ◽  
Zea Mays L ◽  
Germination Percentage ◽  
Phaseolus Lunatus ◽  
Fava Bean ◽  
Randomized Experimental Design ◽  
Salinity Levels ◽  
Osmotic Potentials ◽  
Arid And Semiarid

ABSTRACT Salt stress negatively affects plant development, mainly in arid and semiarid regions, promoting changes in their physiology and productivity. The objective of this study was to evaluate the germinative potential of creole maize (Zea mays L.) and fava bean (Phaseolus lunatus L.) seeds under different salt stress conditions. The seeds were collected in rural areas of the municipalities of Guanambi, Candiba, and Brumado, which are within the Serra Geral region in the state of Bahia, Brazil. A completely randomized experimental design was used; the treatments consisted of three salts (CaCl2, MgCl2, and NaCl) and four salinity levels (osmotic potentials of -0.3, -0.9, and -1.2 MPa). Seed physical analyses included moisture and purity, and physiological analyses included germination percentage, germination speed index, average germination time, and relative germination frequency. The storage of creole maize and fava bean seeds in plastic bottles and under adequate humidity ensures that seeds will have moisture content and physical purity within ideal values. Salt stresses induced by NaCl and CaCl2 salts at osmotic potentials of -0.3 to -1.2 MPa hinder the creole maize and fava bean seed germination, and their germination are null for both salts at osmotic potentials lower than -0.3 MPa. The creole maize and fava bean seeds show germination of 70% under salt stress induced by MgCl2 at osmotic potential of -0.3 MPa, making the germination of both species viable.

Sign in / Sign up

Export Citation Format

Share Document