ANTÍGENO ESPECÍFICO DA PRÓSTATA EM FLUIDOS BIOLÓGICOS: APLICAÇÃO FORENSE

2004 ◽  
Vol 5 (2) ◽  
Author(s):  
Maria Cristina Toledo Sawaya ◽  
Maria Regina Sawaya Rolim
Keyword(s):  
Prostate Specific Antigen ◽  
Seminal Plasma ◽  
Evolutionary History ◽  
Specific Antigen ◽  
Cancer Biomarker ◽  
Forensic Identification ◽  
Prostatic Tissue

O antígeno específico da próstata (PSA) é uma glicoproteína produzida pelo tecido prostático e secretada em altos níveis no fluido seminal. A PSA é amplamente utilizada como marcador prostático tumoral e como marcador forense. Ela foi descrita, primeiramente, como um antígeno específico da próstata e posteriormente sua presença foi observada em vários fluidos extraprostáticos. Esta revisão relata a evolução histórica da PSA e contém informações sobre os níveis de PSA já descritos em fluidos extraprostáticos. O propósito deste artigo é discutir se esses níveis podem interferir na identificação forense do semen. PROSTATE-SPECIFIC ANTIGEN IN BIOLOGICS FLUIDS: FORENSIC APLICATION Abstract Prostate-specific antigen (PSA) is a glycoprotein produced by prostatic tissue and secreted into the seminal plasma at high levels. PSA is widely used as a cancer biomarker and as a forensic marker. Originally PSA was described as a prostate-specific antigen. Later on PSA was observed in a variety of extraprostatic fluids. In this review we summarize its PSA evolutionary history and available informations about levels of PSA in nonprostatic fluids. This article aims to discuss if these levels ccould impair the forensic identification of semen.

Decreased concentrations of prostate-specific antigen and human glandular kallikrein 2 in malignant versus nonmalignant prostatic tissue

Urology ◽  
2000 ◽  
Vol 56 (3) ◽  
pp. 527-532 ◽  
Author(s):  
Angeliki Magklara ◽  
Andreas Scorilas ◽  
Carsten Stephan ◽  
Glen O Kristiansen ◽  
Steffen Hauptmann ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Prostatic Tissue ◽  
Glandular Kallikrein ◽  
Kallikrein 2

scholarly journals High-throughput glycopeptide profiling of prostate-specific antigen from seminal plasma by MALDI-MS

Talanta ◽  
2021 ◽  
Vol 222 ◽  
pp. 121495
Author(s):  
Wei Wang ◽  
Anna Kałuża ◽  
Jan Nouta ◽  
Simone Nicolardi ◽  
Mirosława Ferens-Sieczkowska ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
High Throughput ◽  
Seminal Plasma ◽  
Specific Antigen ◽  
Maldi Ms

scholarly journals Molecular Forms of Prostate-specific Antigen in Malignant and Benign Prostatic Tissue: Biochemical and Diagnostic Implications

2000 ◽  
Vol 46 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Klaus Jung ◽  
Brigitte Brux ◽  
Michael Lein ◽  
Birgit Rudolph ◽  
Glen Kristiansen ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Gel Filtration ◽  
Tumor Stage ◽  
Prostatic Hyperplasia ◽  
Prostatic Tissue ◽  
Molecular Forms ◽  
Tissue Samples ◽  
Total Psa ◽  
Lower Ratio

Abstract Background: Patients with prostate cancer (PCa) show a lower ratio of free prostate-specific antigen (fPSA) to total PSA (tPSA) in serum than patients with benign prostatic hyperplasia (BPH). The patterns of the intracellular PSA isoforms in malignant and benign prostatic tissue have been studied as potential molecular reasons for this phenomenon. Methods: Prostatic tissue samples were obtained after cystoprostatectomy from patients with bladder cancer (n = 10), from BPH patients (transurethral resection of the prostate, n = 10; adenomectomy, n = 10), and from the cancerous and noncancerous parts of the same prostates removed surgically by prostatectomy because of PCa (n = 20). PSA pattern was characterized by gel filtration, immunoblotting, and immunoassays for tPSA, fPSA, α1-antichymotrypsin-PSA (ACT-PSA), and complexed PSA (Bayer Immuno 1 assay). Comparisons were made with the PSA concentrations in serum. Results: The major portion of tPSA in all tissue samples was fPSA; complexed PSA forms were <2%. Samples from cystoprostatectomy patients had the lowest and those from adenomectomy patients the highest values of tPSA and fPSA. PSA concentrations were lower in cancerous than in the noncancerous parts of the prostate. No significant correlations were found between tumor stage or grade and the amounts of tPSA, fPSA, and ACT-PSA in tissue. Tissue PSA values were not correlated with the serum PSA concentrations nor with the ratios fPSA/tPSA and ACT-PSA/tPSA in sera. Conclusions: The amounts of tPSA and the PSA isoforms in prostatic tissue explain neither the concentrations of tPSA and PSA isoforms in serum nor the behavior of the ratio fPSA/tPSA in patients with BPH and PCa.

Fast and novel purification method to obtain the prostate specific antigen (PSA) from human seminal plasma

The Prostate ◽  
2006 ◽  
Vol 66 (10) ◽  
pp. 1029-1036 ◽  
Author(s):  
Boris Acevedo ◽  
Yasser Perera ◽  
Edel Torres ◽  
David Pentón ◽  
Marta Ayala ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Seminal Plasma ◽  
Specific Antigen ◽  
Human Seminal Plasma ◽  
Purification Method

Two step procedure for purification of enzymatically active prostate-specific antigen from seminal plasma

2004 ◽  
Vol 813 (1-2) ◽  
pp. 113-120 ◽  
Author(s):  
B BINDUKUMAR ◽  
E KAWINSKI ◽  
C CHERRIN ◽  
L GAMBINO ◽  
M NAIR ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Seminal Plasma ◽  
Specific Antigen ◽  
Step Procedure

scholarly journals Paper-based electrochemical immunoassay for rapid, inexpensive cancer biomarker protein detection

2014 ◽  
Vol 6 (22) ◽  
pp. 8878-8881 ◽  
Author(s):  
C. K. Tang ◽  
A. Vaze ◽  
J. F. Rusling
Keyword(s):  
Prostate Specific Antigen ◽  
Filter Paper ◽  
Rapid Detection ◽  
Specific Antigen ◽  
Protein Detection ◽  
Cancer Biomarker ◽  
Electrochemical Immunoassay ◽  
Paper Disk ◽  
Filter Paper Disk

Inexpensive, reusable electrochemical chips were configured as immunosensors by using a filter paper disk equipped with antibodies. Rapid detection of cancer biomarker protein prostate specific antigen (PSA) in serum was achieved with 6 pg mL−1 detection in ∼15 min.

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