scholarly journals Paper-based electrochemical immunoassay for rapid, inexpensive cancer biomarker protein detection

2014 ◽  
Vol 6 (22) ◽  
pp. 8878-8881 ◽  
Author(s):  
C. K. Tang ◽  
A. Vaze ◽  
J. F. Rusling
Keyword(s):  
Prostate Specific Antigen ◽  
Filter Paper ◽  
Rapid Detection ◽  
Specific Antigen ◽  
Protein Detection ◽  
Cancer Biomarker ◽  
Electrochemical Immunoassay ◽  
Paper Disk ◽  
Filter Paper Disk

Inexpensive, reusable electrochemical chips were configured as immunosensors by using a filter paper disk equipped with antibodies. Rapid detection of cancer biomarker protein prostate specific antigen (PSA) in serum was achieved with 6 pg mL−1 detection in ∼15 min.

Colorimetric switchable linker-based bioassay for ultrasensitive detection of prostate-specific antigen as a cancer biomarker

The Analyst ◽  
2019 ◽  
Vol 144 (14) ◽  
pp. 4439-4446 ◽  
Author(s):  
Jungwoo Hahn ◽  
Eunghee Kim ◽  
Youngsang You ◽  
Young Jin Choi
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Detection Limits ◽  
Protein Detection ◽  
Critical Issue ◽  
Cancer Biomarker ◽  
Ultrasensitive Detection ◽  
Diagnostic Approaches

The use of colorimetric bioassays for protein detection is one of the most interesting diagnostic approaches, but their relatively poor detection limits have been a critical issue.

Electrochemical immunoassay for the prostate specific antigen using ceria mesoporous nanospheres

2014 ◽  
Vol 181 (13-14) ◽  
pp. 1505-1512 ◽  
Author(s):  
Juan Peng ◽  
Ying-Di Zhu ◽  
Xing-Hua Li ◽  
Li-Ping Jiang ◽  
E. S. Abdel-Halim ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Electrochemical Immunoassay

scholarly journals Assay of prostate-specific antigen from whole blood spotted on filter paper and application to prostate cancer screening

1996 ◽  
Vol 42 (4) ◽  
pp. 536-544 ◽  
Author(s):  
B R Hoffman ◽  
H Yu ◽  
E P Diamandis
Keyword(s):  
Prostate Cancer ◽  
Prostate Specific Antigen ◽  
Filter Paper ◽  
Whole Blood ◽  
Large Scale ◽  
Prostate Cancer Screening ◽  
Specific Antigen ◽  
Blood Concentrations ◽  
Hot Climate ◽  
Plasma Fraction

Abstract We report the measure of prostate-specific antigen (PSA) from extracts of blood dried on filter paper. Five 3-mm (diameter) paper discs containing approximately 25 microL of dried whole blood were punched from the filter paper and extracted with 500 microL of buffer. Recovery of PSA was > 92%. Imprecision of the filter paper procedure was <10% when corresponding whole-blood concentrations were >0.35 micrograms/L. PSA recovery was unaffected whether blood was applied to the filter as one 85-microL aliquots, two 43-microL aliquots, or three 28-microL aliquots. PSA is contained in the plasma fraction. Variation in hematocrit from 0.61 to 0.31 caused <+/-10% change in filter paper PSA. Regression analysis showed: filter paper PSA = 0.86 whole-blood PSA - 0.02; Sy/x = 0.44. Men (153) without prostate cancer gave a 95th percentile of 4.8 micrograms /L. PSA in filter paper dried blood was stable for >1 month at -20 to 37 degrees C and showed no loss of recovery after being mailed to a hot climate. We conclude that the filter paper procedure can reliably distinguish normal from increased concentrations of PSA and that it could facilitate screening to detect occult prostate cancer in large-scale mail-in programs to centralized laboratories.

scholarly journals Rational design of Au nanorods assemblies for highly sensitive and selective SERS detection of prostate specific antigen

RSC Advances ◽  
2015 ◽  
Vol 5 (48) ◽  
pp. 38354-38360 ◽  
Author(s):  
An-qi Yang ◽  
Dong Wang ◽  
Xiang Wang ◽  
Yu Han ◽  
Xue-bin Ke ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Rapid Detection ◽  
Rational Design ◽  
Specific Antigen ◽  
Early Diagnostics ◽  
Au Nanorods ◽  
Highly Sensitive ◽  
Sers Detection

A simple SERS immunosensor based on AuNRs assembly was developed for rapid detection of specific antigen in early diagnostics.

scholarly journals Breeding technique of Selenothrips rubrocinctus (Giard, 1901) (Thysanoptera: Thripidae) on roses leaflets

2021 ◽  
Vol 3 ◽  
pp. ec03054
Author(s):  
Jailma R. Dos Santos ◽  
Brigida Souza ◽  
Marvin M. Pec Hernandez ◽  
Letícia G. de Souza ◽  
Luis Claudio P. Silveira
Keyword(s):  
Population Density ◽  
Filter Paper ◽  
Abiotic Factors ◽  
Petri Dish ◽  
Entire Period ◽  
Distilled Water ◽  
Breeding Technique ◽  
Microcentrifuge Tube ◽  
Paper Disk ◽  
Filter Paper Disk

The development of adequate methods for maintaining populations of arthropod organisms in the laboratory has been a challenge due to the characteristics of each species. This work has aimed to define a method for breeding Selenothrips rubrocinctus (Giard, 1901) in rose leaflets in order to study this species in the laboratory. A condition which could maintain the leaflets turgor for a longer time was sought, in order to guarantee both the survival and multiplication of the insects, and less influence of abiotic factors. Four types of substrates were tested: a) a filter paper disk moistened with distilled water covering the bottom of a Petri dish and; b) a vegetable sponge moistened with distilled water surrounding the base of the leaflet; c) a potato, dextrose and agar (BDA) in a microcentrifuge tube surrounding the base of the leaflet; and d) hydrogel in a microcentrifuge tube surrounding the base of the leaflet. The filter paper moistened with distilled water allowed 65% of the leaflets to remain turgid over a 10-day period and was the most suitable substrate for thrips breeding. With the results at hand, we described S. rubrocinctus breeding in the laboratory. The adopted methodology provided the population density stability of the bred insects, as well as the obtainment of specimens of S. rubrocinctus in quantity and quality throughout the entire period of development of studies on the biology of the species.

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