scholarly journals Mutagen Identification and Molecular Analysis of Pro-vitamin A Carotenoid Genes in Cassava (Manihot esculenta Crantz)

Author(s):  
Lovina I. Udoh ◽  
Adenubi Adesoye ◽  
Melaku Gedil
2015 ◽  
Vol 5 (1) ◽  
pp. 1-10
Author(s):  
Eka Herlina ◽  
Farida Nuraeni

Diversifikasi produk pangan merupakan salah satu cara untuk menunjang ketahanan pangan. Ubi kayu (Manihot esculenta Crantz) dapat digunakan sebagai bahan pangan alternatif pengganti beras yang diolah menjadi flakes. Salah satu komponen bioaktif pada ubikayu yaitu skopoletin suatu senyawa fenolik yang mempunyai aktivitas antioksidan. Penelitian ini dilakukan dengan cara mensubstitusi tepung ubi kayu pada pembuatan flakesubi kayu menggunakan tepung kacang merah dengan berbagai perbandingan tepung ubikayu : tepung kacang merah yaitu 5:0, 4:1, 3:2, 2:3 dan 1:4. Produk olahan dianalisiskandungan vitamin C, A, E, tingkat penerimaan dengan uji organoleptik dan uji aktivitasantioksidan dengan metode DPPH (1,1-difenil-2-pikrilhidrazil). Analisis kadar vitamin Cmenggunakan metode spektrofotometri, sedangkan vitamin A dan E dengan metode HPLC.Hasil penelitian menunjukkan flakes ubi kayu dengan penambahan tepung kacang merahpada formula flakes 3:2 merupakan formulasi yang lebih disukai oleh panelis, dengankandungan vitamin C 5,23 ppm, vitamin A 166,05 IU/100 gram, nilai IC50397,06 ppm, dantidak mengandung vitamin E.


2010 ◽  
Vol 7 (1) ◽  
pp. 105-110
Author(s):  
Madalena Madalena ◽  
Heriyanto Heriyanto ◽  
Susanti Pudji Hastuti ◽  
Leenawaty Limantara

Cassava and ceara-rubber leaves are leavy vegetables that can not be consumed in raw form because they contained cyanide, therefore cooking process is needed to remove the cyanide.  However cooking process cause the changes of  the content of pigments and vitamin A. The aims of the research are to know and to compare the effect of heating time to the content of pigments and vitamin A in cassava and ceara-rubber leaves. Content of chlorophyll and carotenoid was analized base of Porra and Lichtenthaler equations, respectively, while pheophytin content was analyzed base on HPLC. The result shown that the content of chlorophylls, carotenoids and vitamin A of cassava and ceara-rubber leaves were reduced, while the content of pheophytin was increased during heating. Pheophytin was the main product degradation of chlorophyll during heating of cassava and ceara-rubber leaves.   Keywords: heating process, cassava, pigment, vitamin A.


2019 ◽  
Vol 48 (2) ◽  
pp. 148-155
Author(s):  
Jamilah Syafawati Yaacob ◽  
Nur Asniyati Abdul Halim ◽  
Sharmilla Ashokhan ◽  
Hanisah Ali ◽  
Rashidi Othman

Purpose The purpose of this study is to evaluate the pattern of carotenoids distribution in three underutilized Malaysian ‘ulam’ or traditional vegetables in Malaysia (Averrhoa carambola, Manihot esculenta and Ipomoea batatas) and their valuable pro-vitamin A activities. These assessments will yield valuable knowledge and insight into the importance of these underutilized traditional vegetables and highlight their potential for applications in medicinal and functional colorant industries. Design/methodology/approach In this study, the authors have evaluated the distribution of carotenoid compounds in aerial organs of three underutilized traditional vegetables (Averrhoa carambola, Manihot esculenta and Ipomoea batatas). The content of the individual carotenoids were quantified using high-performance liquid chromatography (HPLC) and the organ with the highest accumulation of these compounds were identified. Their valuable pro-vitamin A activities were also reported to indicate their medicinal potential which can further be exploited as pharmacologically active natural colorants and in other applications. Findings In total, three major chromatographic peaks corresponding to lutein, violaxanthin and β-carotene were observed through HPLC. Among the compounds detected, lutein and β-carotene were the most abundant carotenoids found in both shoots and petioles of all three species. Violaxanthin was only detected in I. batatas shoots. Overall, carotenoid content was observed to be higher in the shoots than in the petioles, where I. batatas contained the highest amount of total carotenoid, followed by M. esculenta and A. carambola. The opposite trend was observed in the petioles, where A. carambola petioles had the highest carotenoid content, while I. batatas contained the least. Research limitations/implications The distribution and abundance of these individual carotenoids suggested that the petioles contained the highest amount of carotenoid, contributing to its high pro-vitamin A activity, and could be potentially useful for medicinal application, as it can act as storage site that is not as prone to natural drying or degradation during harvest and sample storage. Future research work should include improvements in the extraction and purification procedures as well as robust identification methods which may lead to better detection and identification of other compounds that could attribute to its bioactivity, to complement the findings of the current study. Practical implications This analysis provides valuable information on the importance of underutilized traditional vegetables as important biofactories for sustainable production of valuable pigments (such as carotenoids) with medicinal benefits and can further be exploited in various industries, such as in formulation of functional natural colorants. This study also highlights the importance of petiole as a storage site of pharmacologically active compounds that is not as prone to natural drying or degradation during harvest and sample storage. Originality/value To date, there is no previous report found on comparative analysis of carotenoid content and quantification of individual carotenoid concentration in the edible aerial parts of Averrhoa carambola, Manihot esculenta and Ipomoea batatas, although they have been traditionally consumed as “ulam” in Malaysia. Therefore, the results reported in this study provide new insights on carotenoid accumulation in the selected ‘ulam’ species.


2015 ◽  
Vol 12 (4) ◽  
pp. 27-38
Author(s):  
Rodolfo Moresco ◽  
Virgílio G. Uarrota ◽  
Aline Pereira ◽  
Maíra Tomazzoli ◽  
Eduardo da C. Nunes ◽  
...  

Summary In this study, the metabolomics characterization focusing on the carotenoid composition of ten cassava (Manihot esculenta) genotypes cultivated in southern Brazil by UV-visible scanning spectrophotometry and reverse phase-high performance liquid chromatography was performed. Cassava roots rich in β-carotene are an important staple food for populations with risk of vitamin A deficiency. Cassava genotypes with high pro-vitamin A activity have been identified as a strategy to reduce the prevalence of deficiency of this vitamin. The data set was used for the construction of a descriptive model by chemometric analysis. The genotypes of yellow-fleshed roots were clustered by the higher concentrations of cis- β-carotene and lutein. Inversely, cream-fleshed roots genotypes were grouped precisely due to their lower concentrations of these pigments, as samples rich in lycopene (redfleshed) differed among the studied genotypes. The analytical approach (UV-Vis, HPLC, and chemometrics) used showed to be efficient for understanding the chemodiversity of cassava genotypes, allowing to classify them according to important features for human health and nutrition.


Author(s):  
Egle Machado de Almeida Siqueira ◽  
Sandra Fernandes Arruda ◽  
Rodrigo Martins de Vargas ◽  
Elizabeth Maria Talá de Souza

2018 ◽  
Vol 9 (9) ◽  
pp. 4822-4835 ◽  
Author(s):  
Ingrid J. Aragón ◽  
Hernán Ceballos ◽  
Dominique Dufour ◽  
Mario G. Ferruzzi

The effects of fermentation and thermal processing on the stability and bioaccessibility of pVAC from elite selections of biofortified cassava roots were assessed to determine the potential for genotype x processing effects.


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